##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043315_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2484207
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27597458665884	33.0	31.0	34.0	31.0	34.0
2	32.600544157552086	34.0	31.0	34.0	31.0	34.0
3	32.70130508447968	34.0	31.0	34.0	31.0	34.0
4	36.20780514667256	37.0	37.0	37.0	35.0	37.0
5	36.20157982003915	37.0	37.0	37.0	35.0	37.0
6	36.30835634872617	37.0	37.0	37.0	35.0	37.0
7	36.24875463276611	37.0	37.0	37.0	35.0	37.0
8	36.17447499342849	37.0	37.0	37.0	35.0	37.0
9	37.770505034403335	39.0	38.0	39.0	35.0	39.0
10	37.65136681444018	39.0	37.0	39.0	35.0	39.0
11	37.48508276484206	39.0	37.0	39.0	35.0	39.0
12	37.5113575479016	39.0	37.0	39.0	35.0	39.0
13	37.537668962369075	39.0	37.0	39.0	35.0	39.0
14	38.94116673852058	41.0	38.0	41.0	35.0	41.0
15	38.99393689817314	41.0	38.0	41.0	35.0	41.0
16	38.94382070415227	41.0	38.0	41.0	35.0	41.0
17	38.90095873653041	41.0	38.0	41.0	35.0	41.0
18	38.633166640300104	40.0	38.0	41.0	35.0	41.0
19	38.34777335383082	40.0	37.0	41.0	35.0	41.0
20	38.05719893712561	40.0	35.0	41.0	35.0	41.0
21	38.00123459921013	40.0	35.0	41.0	35.0	41.0
22	37.9501680012978	40.0	35.0	41.0	34.0	41.0
23	37.92484482975855	40.0	35.0	41.0	34.0	41.0
24	37.818860103042944	40.0	35.0	41.0	34.0	41.0
25	37.789713981161796	40.0	35.0	41.0	34.0	41.0
26	37.75852092840894	40.0	35.0	41.0	34.0	41.0
27	37.73063879137286	40.0	35.0	41.0	34.0	41.0
28	37.63133990041893	39.0	35.0	41.0	34.0	41.0
29	37.57248208381991	39.0	35.0	41.0	34.0	41.0
30	37.44231982278449	39.0	35.0	41.0	34.0	41.0
31	37.265654995739084	39.0	35.0	41.0	33.0	41.0
32	36.97966876351286	39.0	35.0	41.0	33.0	41.0
33	36.58214713991225	39.0	35.0	41.0	31.0	41.0
34	36.17395732320213	39.0	35.0	41.0	30.0	41.0
35	35.96022674438966	39.0	35.0	41.0	30.0	41.0
36	35.81799705097039	39.0	35.0	41.0	29.0	41.0
37	35.63525100766562	38.0	35.0	41.0	28.0	41.0
38	35.49656248452725	38.0	35.0	41.0	27.0	41.0
39	35.43276264820122	38.0	35.0	41.0	27.0	41.0
40	35.35004208586483	38.0	35.0	40.0	26.0	41.0
41	35.25402110210622	38.0	35.0	40.0	26.0	41.0
42	35.17476361671954	38.0	35.0	40.0	25.0	41.0
43	35.059003537144854	38.0	35.0	40.0	24.0	41.0
44	34.92520832603724	37.0	35.0	40.0	24.0	41.0
45	34.879062413075886	37.0	35.0	40.0	23.0	41.0
46	34.787955673581145	37.0	35.0	40.0	23.0	41.0
47	34.65415120398582	37.0	35.0	40.0	23.0	41.0
48	34.58132756247768	37.0	35.0	40.0	23.0	41.0
49	34.51102746268729	36.0	34.0	40.0	24.0	41.0
50	34.44314906124973	36.0	34.0	40.0	24.0	41.0
51	34.025089294088616	36.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	10.0
14	22.0
15	28.0
16	83.0
17	169.0
18	400.0
19	844.0
20	1500.0
21	2599.0
22	4123.0
23	6586.0
24	11339.0
25	21435.0
26	36696.0
27	48146.0
28	50883.0
29	48337.0
30	45782.0
31	49020.0
32	55858.0
33	71039.0
34	140657.0
35	214834.0
36	238995.0
37	231956.0
38	379991.0
39	822707.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.723773824000983	16.257018839412336	21.95561803022051	33.063589306366175
2	38.57887044034575	18.609882348773674	22.213929837569896	20.597317373310677
3	25.472595480167314	19.579487538679345	35.63845524950216	19.309461731651187
4	21.788079656807987	22.283046461104085	34.29617580177498	21.632698080312952
5	20.518016413286013	30.347913841318373	31.43494080807276	17.699128937322854
6	74.3478703666804	1.9933524058180336	20.444471817364658	3.214305410136917
7	73.48908524933712	1.9547082831664189	19.21905058636418	5.337155881132289
8	64.1234405989517	3.427411644842801	24.977507912987928	7.471639843217575
9	36.34681006856514	14.933336875711243	30.681138890599698	18.03871416512392
10	25.093359772353914	15.698208724152215	38.71001893159467	20.498412571899202
11	22.7746721589626	12.533617367634822	42.704251296288916	21.987459177113664
12	19.30930071447347	13.175834380951345	45.739103061862394	21.775761842712786
13	15.755007533591204	16.78181407588015	44.79445553450256	22.66872285602609
14	15.239873327786292	17.47173242809476	45.43751788800209	21.85087635611686
15	18.176544869248012	14.157918402129935	45.43755814229652	22.227978586325538
16	18.08118244574627	18.44894567964747	41.43378550982265	22.03608636478361
17	18.43888210604028	19.96121901274733	40.605794927717376	20.99410395349502
18	19.140232677872657	25.033662653716053	37.09344672162988	18.73265794678141
19	19.717761040042152	20.556741044526483	36.41878474700378	23.30671316842759
20	20.318636892980336	24.797852996952347	35.82012288025918	19.063387229808143
21	17.68338950820121	19.943466868904242	36.441005117528455	25.932138505366098
22	18.457882133010656	22.408720368310693	35.329946337000095	23.803451161678556
23	19.177830188869123	19.7807992651176	38.528230537954364	22.51314000805891
24	22.392055090417184	18.51729747158751	35.8377945155134	23.252852922481903
25	18.02764423415601	18.71534860017704	38.68244473991096	24.574562425755985
26	18.079652782557975	17.57281096140539	38.82808477715424	25.519451478882395
27	21.227619115476287	18.823350872129417	38.90621836264047	21.042811649753826
28	21.213852146781647	20.670258154815603	34.516890098127895	23.598999600274855
29	20.990400558407572	18.434051590708826	37.83867447438961	22.73687337649399
30	22.800837450341298	18.099216369650357	35.2832111011683	23.816735078840047
31	22.575212130068063	17.37588695305987	34.389042459022136	25.659858457849932
32	20.78152102461671	18.49588218695141	35.54731952691543	25.175277261516456
33	17.99379037254142	18.106140108292106	37.692309859846624	26.20775965931986
34	18.51641187711008	19.867345998139445	34.73969761779111	26.876544506959366
35	18.849153874858253	22.01233633107064	31.598936803575544	27.539572990495557
36	21.13914017632186	21.661640918007237	29.247683465991358	27.951535439679542
37	20.16933371494404	21.886501406686317	30.01577565798663	27.928389220383004
38	20.665186113717578	19.07264571752676	28.46723320560646	31.794934963149206
39	23.08640141501896	17.752385368852114	33.528767932784994	25.63244528334394
40	20.916654691014074	17.179848539191784	36.1900195917651	25.71347717802904
41	20.907476711884314	21.36259176469594	32.62248274801576	25.107448775403984
42	20.138861214061468	17.75963114184929	34.567087203280565	27.534420440808677
43	22.6944856044605	19.35144696074039	34.58246434375235	23.37160309104676
44	21.156892320164946	19.63572278799633	35.704150258009896	23.503234633828825
45	21.72797999522584	19.469392043416672	34.035650008232004	24.766977953125487
46	22.37921397049441	18.531547491815296	33.65983591544505	25.429402622245245
47	20.383446307010647	19.71043475845612	34.42587513842446	25.480243796108777
48	21.08024814357258	18.190271583648222	32.149414279888916	28.580065992890287
49	20.10198828036472	16.19462468304775	36.57311971184366	27.130267324743873
50	19.91810666341412	16.262855712104507	37.62898985471018	26.190047769771198
51	18.337320521196503	15.489127918889206	37.601979223148476	28.571572336765815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	526.0
1	3381.5
2	6237.0
3	94062.0
4	181887.0
5	105719.5
6	29552.0
7	26875.0
8	24198.0
9	23039.5
10	21881.0
11	20853.5
12	19826.0
13	18562.0
14	17298.0
15	16171.5
16	15045.0
17	13878.0
18	12711.0
19	11741.5
20	10772.0
21	10226.0
22	9680.0
23	9204.0
24	8728.0
25	8725.0
26	9334.0
27	9946.0
28	10505.0
29	11064.0
30	13429.0
31	15794.0
32	18277.0
33	20760.0
34	23129.0
35	25498.0
36	27752.0
37	30006.0
38	32474.0
39	34942.0
40	39515.0
41	44088.0
42	49520.5
43	54953.0
44	63679.0
45	72405.0
46	86014.5
47	99624.0
48	140090.0
49	180556.0
50	221161.0
51	261766.0
52	267910.5
53	274055.0
54	242450.0
55	210845.0
56	182784.0
57	154723.0
58	138199.5
59	121676.0
60	115606.0
61	109536.0
62	104615.0
63	99694.0
64	96597.0
65	93500.0
66	76888.5
67	60277.0
68	50647.0
69	41017.0
70	35396.5
71	29776.0
72	27198.0
73	24620.0
74	20949.0
75	13476.5
76	9675.0
77	7040.0
78	4405.0
79	3426.0
80	2447.0
81	1825.0
82	1203.0
83	878.5
84	554.0
85	436.0
86	318.0
87	196.0
88	74.0
89	54.0
90	34.0
91	27.5
92	21.0
93	13.0
94	5.0
95	6.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2484207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.736709646363902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2902918934689	22.834391314731757
2	9.230304274072257	5.674183648395013
3	3.5189861402190434	3.2448616572447464
4	2.0277864918862507	2.4930993850370613
5	1.3969339801985263	2.1468577072250783
6	1.1104357690681381	2.0478685088870496
7	0.9168511968257298	1.9726692318027583
8	0.7871794550346631	1.9356245079186725
9	0.7101629986443871	1.964526650183128
>10	5.64238234768095	30.093776472223926
>50	0.19946802976611855	4.331691538719442
>100	0.15758538755371915	8.80530560252368
>500	0.005948200013201345	1.231814458796238
>1k	0.004626377788045491	2.9745318767719575
>5k	6.609111125779273E-4	1.4783073616464895
>10k+	3.965466675467564E-4	6.770490077893081
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	137862	5.549537538538455	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	17091	0.6879861460820295	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	11691	0.4706129561666963	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	8886	0.3576996602940093	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	7675	0.3089517097407744	No Hit
CGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTG	6936	0.2792037861579168	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	6637	0.2671677521237159	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTT	6252	0.25166984876864124	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4878	0.19636044822351761	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	4598	0.18508924578346328	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT	4450	0.17913161020800603	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3926	0.15803835992733295	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	3554	0.14306376239983223	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCC	3437	0.1383540099516667	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	3219	0.1295785737661958	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2937	0.11822686273728397	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTC	2752	0.11077981826796238	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2721	0.10953193514067064	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2604	0.1048221826925051	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3166805342710974	0.0
2	0.0	0.0	0.0	0.987478096632044	0.0
3	0.0	0.0	0.0	1.7099219187450965	0.0
4	0.0	0.0	0.0	2.2218760352901348	0.0
5	0.0	0.0	0.0	4.6968308196539175	0.0
6	0.0	0.0	0.0	5.950148276693528	0.0
7	0.0	0.0	0.0	9.047474707220452	0.0
8	0.0	0.0	0.0	12.261659354474084	0.0
9	0.0	0.0	0.0	15.788901649500223	0.0
10	0.0	0.0	0.0	17.49395279861944	0.0
11	0.0	0.0	0.0	18.75041009062449	0.0
12	0.0	0.0	0.0	19.610765125450495	0.0
13	0.0	0.0	0.0	20.050221257729326	0.0
14	4.0254294428765397E-5	0.0	0.0	20.344037352764886	0.0
15	4.0254294428765397E-5	0.0	0.0	20.999135740298616	0.0
16	4.0254294428765397E-5	0.0	0.0	21.678306195900745	0.0
17	4.0254294428765397E-5	0.0	0.0	22.604678273589922	0.0
18	4.0254294428765397E-5	0.0	0.0	23.084187428825377	0.0
19	4.0254294428765397E-5	0.0	0.0	23.671819618896492	0.0
20	8.050858885753079E-5	0.0	0.0	24.262873423994055	0.0
21	8.050858885753079E-5	0.0	0.0	24.890880671377225	0.0
22	8.050858885753079E-5	0.0	0.0	25.53003835831716	0.0
23	8.050858885753079E-5	0.0	0.0	26.072706501511348	0.0
24	8.050858885753079E-5	0.0	0.0	26.52806308008954	0.0
25	8.050858885753079E-5	0.0	0.0	26.95165902036344	0.0
26	8.050858885753079E-5	0.0	0.0	27.390591846814697	0.0
27	8.050858885753079E-5	0.0	0.0	27.85420055575079	0.0
28	8.050858885753079E-5	0.0	0.0	28.311690611933706	0.0
29	8.050858885753079E-5	0.0	0.0	28.77690949264695	0.0
30	8.050858885753079E-5	0.0	0.0	29.202840181997715	0.0
31	8.050858885753079E-5	0.0	0.0	29.632353503552643	0.0
32	8.050858885753079E-5	0.0	0.0	30.11137960725495	0.0
33	8.050858885753079E-5	0.0	0.0	30.55683362940367	0.0
34	8.050858885753079E-5	0.0	0.0	31.082836494704345	0.0
35	8.050858885753079E-5	0.0	0.0	31.520118895084025	0.0
36	8.050858885753079E-5	0.0	0.0	31.977649205561374	0.0
37	8.050858885753079E-5	0.0	0.0	32.50365207086205	0.0
38	8.050858885753079E-5	4.0254294428765397E-5	0.0	33.0478096229501	0.0
39	8.050858885753079E-5	4.0254294428765397E-5	0.0	33.6408761427691	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35645	0.0	44.394024	1
CGAAACG	75	0.0	42.0	13
TACGGGT	205	0.0	41.707314	3
CACTTAC	4210	0.0	41.31235	36
ACTTACT	4140	0.0	41.304348	37
ACGGGTA	285	0.0	41.05263	4
CGCACTT	4270	0.0	40.942623	34
ACCGGTA	165	0.0	40.90909	41
TCGGCGT	1765	0.0	40.793198	4
CCGCACT	4305	0.0	40.609756	33
GCACTTA	4295	0.0	40.599537	35
TTAACGG	100	0.0	40.5	1
ACGGGAT	335	0.0	40.298508	4
CTTACTG	4250	0.0	40.235294	38
CCCGCAC	4430	0.0	40.22573	32
TAGTAGG	565	0.0	40.221237	1
GTTTTTT	39795	0.0	40.216736	2
ACCCGCA	4420	0.0	40.214935	31
GTGGCGT	1625	0.0	40.153847	34
GTCCCCC	1930	0.0	39.870464	9
>>END_MODULE