FastQCFastQC Report
Thu 26 May 2016
SRR1043314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043314_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2416470
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1023914.237213787053015No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC122210.5057377083100556No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT80960.3350341614007209TruSeq Adapter, Index 16 (100% over 24bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCTT69460.2874440816563003TruSeq Adapter, Index 16 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA69360.28703025487591405No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT48490.20066460580930034TruSeq Adapter, Index 16 (100% over 24bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC41190.1704552508411029TruSeq Adapter, Index 16 (95% over 24bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38720.16023372936556216No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC37120.1536125008793819No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCG30020.12423079947195703No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT29630.12261687502845059TruSeq Adapter, Index 16 (100% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC26530.10978824483647634No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA25540.10569135971065231No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCT24840.10279457224794845TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT260900.045.1536941
TATACGG1000.043.71
CTTGCGG6600.043.5606041
TCGATGG2300.043.01
CGACGGT2200.042.86363627
AATACGG700.042.7142831
ATCACGG2000.042.5500031
TAGTAGG5950.042.5210081
GCATAGG4200.042.1666681
TACGGGA3000.041.43
CACTTAC35100.041.15099736
TACGGGT1400.041.0714263
ACTACGG1850.041.0270271
GCACTTA35350.040.990135
TAGCATA10500.040.96190329
GTTTTTT294650.040.9027632
CATGCGG3600.040.8888851
CGCACTT35600.040.8314634
GTAGCAT10700.040.62616728
TAAACGG850.040.5882341
Produced by FastQC (version 0.11.5)