FastQCFastQC Report
Thu 26 May 2016
SRR1043313_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043313_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2383238
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT979424.109618930211754No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC120850.5070832203917527No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC78360.3287963686379623TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT66850.28050073051873126TruSeq Adapter, Index 16 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG58820.246807075080206No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC50280.21097347390399113TruSeq Adapter, Index 16 (100% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG41050.17224465202384318TruSeq Adapter, Index 16 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37340.1566775957751597No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37110.15571252220718199No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC30330.12726383181201373No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC29550.12399097362495899No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC25100.10531889807060815No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC24040.10087116771384141TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG850.045.01
CGTTTTT242800.044.462521
CATGCGG2950.043.4745751
CACTTAC35450.041.0014136
TAGTAGG6100.040.9426231
GCACTTA35400.040.932235
CGCACTT35500.040.81690234
ACTACGG1550.040.645161
CTTGCGG6500.040.51
AATTCGG501.0822987E-940.51
GTTTTTT273750.040.4219172
TAACGGG6100.040.2049182
CTAACGG3200.040.078131
TTATCGG451.9297659E-840.0000041
ACGTAGG1800.040.0000041
TTCACGG2250.040.01
AGGGCTT10700.039.953275
GGACTAA4850.039.8969047
CCGCACT36450.039.75308633
ACTTACT35950.039.74269537
Produced by FastQC (version 0.11.5)