##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043313_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2383238
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.312355291414455	33.0	31.0	34.0	31.0	34.0
2	32.59450378015121	34.0	31.0	34.0	31.0	34.0
3	32.68756582431129	34.0	31.0	34.0	31.0	34.0
4	36.1863447964492	37.0	37.0	37.0	35.0	37.0
5	36.163425138404136	37.0	37.0	37.0	35.0	37.0
6	36.33280813750032	37.0	37.0	37.0	35.0	37.0
7	36.28021834160079	37.0	37.0	37.0	35.0	37.0
8	36.183181872729456	37.0	37.0	37.0	35.0	37.0
9	37.793701258539855	39.0	38.0	39.0	35.0	39.0
10	37.535930108533016	39.0	37.0	39.0	35.0	39.0
11	37.02572130857262	39.0	35.0	39.0	34.0	39.0
12	36.98997666200354	39.0	35.0	39.0	34.0	39.0
13	36.949260208170564	39.0	35.0	39.0	33.0	39.0
14	38.14501489150475	40.0	35.0	41.0	34.0	41.0
15	38.215811849257186	40.0	35.0	41.0	34.0	41.0
16	38.074520043738815	40.0	35.0	41.0	33.0	41.0
17	37.97766190367894	40.0	36.0	41.0	33.0	41.0
18	37.89286970080202	39.0	36.0	41.0	34.0	41.0
19	37.73248034816498	39.0	35.0	41.0	34.0	41.0
20	37.4998426510487	39.0	35.0	41.0	34.0	41.0
21	37.446429605435966	39.0	35.0	41.0	34.0	41.0
22	37.42236402742823	39.0	35.0	41.0	34.0	41.0
23	37.40881271614501	39.0	35.0	41.0	34.0	41.0
24	37.33568657431612	39.0	35.0	41.0	34.0	41.0
25	37.28962906768019	39.0	35.0	41.0	34.0	41.0
26	37.261356188513275	39.0	35.0	41.0	34.0	41.0
27	37.228053597668385	39.0	35.0	41.0	34.0	41.0
28	37.13656210584087	38.0	35.0	41.0	33.0	41.0
29	37.05370592446076	38.0	35.0	41.0	33.0	41.0
30	36.927608572874384	38.0	35.0	41.0	33.0	41.0
31	36.8004815297507	38.0	35.0	41.0	33.0	41.0
32	36.60894253951976	38.0	35.0	41.0	32.0	41.0
33	36.14963801349257	38.0	35.0	41.0	31.0	41.0
34	35.9028477223005	38.0	35.0	41.0	30.0	41.0
35	35.807452298091924	38.0	35.0	41.0	30.0	41.0
36	35.76082539805089	38.0	35.0	41.0	30.0	41.0
37	35.65910580479163	38.0	35.0	40.0	30.0	41.0
38	35.63245089244129	37.0	35.0	40.0	30.0	41.0
39	35.607902358052364	37.0	35.0	40.0	30.0	41.0
40	35.56540555328507	37.0	35.0	40.0	30.0	41.0
41	35.488087635393526	37.0	35.0	40.0	30.0	41.0
42	35.366226537173375	37.0	35.0	40.0	29.0	41.0
43	35.214459907067614	36.0	35.0	40.0	29.0	41.0
44	35.08724139175357	36.0	35.0	40.0	28.0	41.0
45	35.00666823875752	36.0	35.0	40.0	27.0	41.0
46	34.92590920420034	36.0	35.0	40.0	27.0	41.0
47	34.820073362375055	35.0	35.0	40.0	27.0	41.0
48	34.77196360581696	35.0	35.0	40.0	27.0	41.0
49	34.70069795798825	35.0	34.0	40.0	27.0	41.0
50	34.63287384642239	35.0	34.0	40.0	27.0	41.0
51	34.247431435718966	35.0	34.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	2.0
13	5.0
14	22.0
15	40.0
16	109.0
17	217.0
18	523.0
19	1017.0
20	1689.0
21	2802.0
22	4358.0
23	6475.0
24	10237.0
25	17553.0
26	27724.0
27	36603.0
28	41293.0
29	44600.0
30	48756.0
31	55528.0
32	64188.0
33	83467.0
34	211783.0
35	288156.0
36	204993.0
37	208989.0
38	327750.0
39	694162.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.26237329213448	17.50030840394455	24.477622461541817	28.759695842379152
2	34.022283968281805	19.938797551902077	24.98323709172143	21.055681388094687
3	25.81827748634421	21.41670282195903	33.07537895921431	19.689640732482445
4	23.373368501173612	23.525892084634435	31.0437312597399	22.057008154452053
5	21.944472184481782	30.98951930105176	28.214471236192107	18.851537278274346
6	78.99018058624443	1.9368187314905183	16.110560506336338	2.9624401759287156
7	78.96861328998614	1.7253837006627117	14.721022407329858	4.584980602021284
8	70.0654319879089	2.8531770641455028	20.044913684659274	7.0364772632863355
9	46.489943513824464	13.01963966670555	23.694947797911915	16.795469021558066
10	34.47112709683213	13.606152637713901	32.551763609005896	19.370956656448076
11	31.220801279603634	11.163635356603075	36.46198994812939	21.1535734156639
12	23.97301486465053	12.780846898211593	39.35888904087632	23.887249196261557
13	17.320091405054804	15.254498291819784	44.371187434909984	23.054222868215426
14	20.040088316819386	14.976850822284641	43.9272116339199	21.055849226976072
15	25.889273333171086	12.9389091647582	40.98734578753779	20.18447171453292
16	23.13772271170567	16.756656280237223	38.799440089491696	21.306180918565413
17	23.264818704636298	18.401603196995012	38.656819000032726	19.67675909833596
18	23.251811191328773	26.066259433594123	33.36376811715825	17.31816125791885
19	24.424837133345473	23.198648225649308	30.838380388362385	21.53813425264283
20	23.209096196015672	27.370115783652327	31.5862284841044	17.834559536227605
21	19.335962249678797	21.444815834591427	35.74091215396867	23.4783097617611
22	23.083510753017535	20.57335440270758	33.09233068623445	23.25080415804045
23	25.60667461663502	18.33941889144097	33.94251853990243	22.111387952021577
24	25.394190592798537	16.540311962128833	31.663476329262963	26.402021115809664
25	17.675574155833367	17.37191165968317	36.84617314762521	28.106341036858257
26	16.775957751596778	17.0785712547383	37.00385777668869	29.141613216976232
27	20.501183683710984	21.67215359943069	34.107881797789396	23.71878091906893
28	24.121174637195278	22.72454534545018	30.017690218098238	23.136589799256306
29	23.48380648512654	18.39123914606934	33.16542451907867	24.959529849725456
30	27.366926844905965	17.074543121585002	32.05655498947231	23.501975044036726
31	29.377217046723825	17.87236524426012	27.892724100572412	24.857693608443636
32	26.74281796446683	16.36768967262187	29.193181713282517	27.69631064962878
33	20.099335441949144	18.87285281621055	34.81414781066767	26.213663931172633
34	17.51444882970144	23.788266215963326	32.362483310521235	26.334801643814004
35	19.178907016420517	26.99369513242068	28.8577976685501	24.969600182608705
36	21.851699242794886	22.861963429586137	25.665166466798535	29.621170860820445
37	20.44478981956481	24.848126792204557	28.377946306663453	26.32913708156718
38	20.150316502170575	20.50953366806001	28.35919031166841	30.980959518101002
39	21.47024342512162	16.46952591390369	32.40423323226636	29.655997428708336
40	16.752334429041497	18.110654496109916	34.20052886031525	30.936482214533335
41	18.332537497304088	21.18210602549976	29.80503835538037	30.68031812181578
42	18.16717423941713	16.057271661495832	32.5917931822168	33.18376091687024
43	20.684170024143622	17.255725194042725	32.492810201918566	29.56729457989508
44	21.39899581997266	18.623276399587454	32.28275984186221	27.69496793857768
45	21.74730345857191	20.65530173654499	32.14194302037816	25.455451784504945
46	20.65765148088441	19.438847483969287	31.74051437581979	28.16298665932651
47	18.649543184524582	18.461437758209627	35.004267303559274	27.88475175370651
48	21.25033253078375	16.850604094093832	32.08999688658875	29.80906648853367
49	20.43941897536041	15.844451959896578	35.016435622459866	28.69969344228315
50	18.800346419451184	15.596889609850129	37.34545186003244	28.257312110666245
51	17.181918045952607	14.66122980583559	36.33078190260478	31.826070245607024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	667.0
1	4080.0
2	7493.0
3	75256.0
4	143019.0
5	81411.5
6	19804.0
7	16714.5
8	13625.0
9	13019.5
10	12414.0
11	11982.5
12	11551.0
13	10959.0
14	10367.0
15	9474.5
16	8582.0
17	8026.5
18	7471.0
19	6889.5
20	6308.0
21	5939.0
22	5570.0
23	5519.5
24	5469.0
25	5326.5
26	6459.5
27	7735.0
28	7738.5
29	7742.0
30	9034.0
31	10326.0
32	12107.5
33	13889.0
34	15532.5
35	17176.0
36	18516.0
37	19856.0
38	22924.5
39	25993.0
40	30440.5
41	34888.0
42	41307.5
43	47727.0
44	54778.0
45	61829.0
46	71816.0
47	81803.0
48	115723.0
49	149643.0
50	190376.5
51	231110.0
52	246513.5
53	261917.0
54	242423.5
55	222930.0
56	200516.5
57	178103.0
58	163454.0
59	148805.0
60	147673.5
61	146542.0
62	142606.0
63	138670.0
64	130116.0
65	121562.0
66	98129.0
67	74696.0
68	57609.0
69	40522.0
70	33854.5
71	27187.0
72	25275.5
73	23364.0
74	19111.0
75	11368.0
76	7878.0
77	6314.0
78	4750.0
79	3400.0
80	2050.0
81	1549.0
82	1048.0
83	867.5
84	687.0
85	484.5
86	282.0
87	186.0
88	90.0
89	56.5
90	23.0
91	23.0
92	23.0
93	14.5
94	6.0
95	4.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2383238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.84509007509563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.56525436681352	20.20586044166149
2	10.67089011689803	5.942637929729468
3	4.018147241363153	3.3565701561226207
4	2.0455339675566337	2.2783231031312887
5	1.2789105735653	1.7805690059458996
6	0.9409972209235137	1.5721291426217945
7	0.7152548823575198	1.3941435638127946
8	0.622013918732477	1.385602687605523
9	0.5560494845614686	1.3934923185442134
>10	6.017773850109585	35.28151608664372
>50	0.3784725637958438	7.023775347902049
>100	0.17686380719136963	8.498478917670521
>500	0.007755366073721022	1.4623972579832156
>1k	0.005170244049147348	2.688817450247334
>5k	6.082640057820409E-4	1.0768226733002622
>10k+	3.0413200289102047E-4	4.658863917077895
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97942	4.109618930211754	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12085	0.5070832203917527	No Hit
CCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	7836	0.3287963686379623	TruSeq Adapter, Index 16 (100% over 23bp)
CTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGCT	6685	0.28050073051873126	TruSeq Adapter, Index 16 (96% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	5882	0.246807075080206	No Hit
GCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTGC	5028	0.21097347390399113	TruSeq Adapter, Index 16 (100% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTCTG	4105	0.17224465202384318	TruSeq Adapter, Index 16 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3734	0.1566775957751597	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3711	0.15571252220718199	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	3033	0.12726383181201373	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCC	2955	0.12399097362495899	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2510	0.10531889807060815	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACCCTCTCGTATGCCGTCTTC	2404	0.10087116771384141	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29162005641064803	0.0
2	0.0	0.0	0.0	1.0001518941876557	0.0
3	0.0	0.0	0.0	1.632484879814773	0.0
4	0.0	0.0	0.0	2.080740572280234	0.0
5	0.0	0.0	0.0	4.378622697355446	0.0
6	0.0	0.0	0.0	5.326576699431613	0.0
7	0.0	0.0	0.0	7.89879147613457	0.0
8	0.0	0.0	0.0	11.13342435795334	0.0
9	0.0	0.0	0.0	14.21897435337973	0.0
10	0.0	0.0	0.0	15.986359734109644	0.0
11	0.0	0.0	0.0	17.292943465990387	0.0
12	0.0	0.0	0.0	18.1488798013459	0.0
13	0.0	0.0	0.0	18.650214540050133	0.0
14	0.0	0.0	0.0	18.974101621407513	0.0
15	0.0	0.0	0.0	19.659052096349587	0.0
16	0.0	0.0	0.0	20.3862140499606	0.0
17	0.0	0.0	0.0	21.310586689201834	0.0
18	0.0	0.0	0.0	21.90532376539817	0.0
19	0.0	0.0	0.0	22.571560205065545	0.0
20	0.0	0.0	0.0	23.301155822456675	0.0
21	0.0	0.0	0.0	24.011366049047556	0.0
22	4.195972034685583E-5	0.0	0.0	24.64642641649722	0.0
23	8.391944069371166E-5	0.0	0.0	25.252744375509288	0.0
24	1.2587916104056748E-4	0.0	0.0	25.767883862207636	0.0
25	1.2587916104056748E-4	0.0	0.0	26.25772163753683	0.0
26	1.2587916104056748E-4	0.0	0.0	26.73002024976104	0.0
27	1.2587916104056748E-4	0.0	0.0	27.232571820355332	0.0
28	1.6783888138742332E-4	0.0	0.0	27.70579354642717	0.0
29	1.6783888138742332E-4	0.0	0.0	28.213002645979966	0.0
30	1.6783888138742332E-4	0.0	0.0	28.687315324780823	0.0
31	1.6783888138742332E-4	0.0	0.0	29.196286732588185	0.0
32	1.6783888138742332E-4	0.0	0.0	29.774156001205082	0.0
33	1.6783888138742332E-4	0.0	0.0	30.312583132695938	0.0
34	1.6783888138742332E-4	0.0	0.0	30.884200402981154	0.0
35	1.6783888138742332E-4	0.0	0.0	31.38310147790527	0.0
36	1.6783888138742332E-4	0.0	0.0	31.91368214169126	0.0
37	1.6783888138742332E-4	0.0	0.0	32.69555957063457	0.0
38	1.6783888138742332E-4	0.0	0.0	33.533243427639206	0.0
39	2.0979860173427916E-4	0.0	0.0	34.44796533120066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	85	0.0	45.0	1
CGTTTTT	24280	0.0	44.46252	1
CATGCGG	295	0.0	43.474575	1
CACTTAC	3545	0.0	41.00141	36
TAGTAGG	610	0.0	40.942623	1
GCACTTA	3540	0.0	40.9322	35
CGCACTT	3550	0.0	40.816902	34
ACTACGG	155	0.0	40.64516	1
CTTGCGG	650	0.0	40.5	1
AATTCGG	50	1.0822987E-9	40.5	1
GTTTTTT	27375	0.0	40.421917	2
TAACGGG	610	0.0	40.204918	2
CTAACGG	320	0.0	40.07813	1
TTATCGG	45	1.9297659E-8	40.000004	1
ACGTAGG	180	0.0	40.000004	1
TTCACGG	225	0.0	40.0	1
AGGGCTT	1070	0.0	39.95327	5
GGACTAA	485	0.0	39.896904	7
CCGCACT	3645	0.0	39.753086	33
ACTTACT	3595	0.0	39.742695	37
>>END_MODULE