FastQCFastQC Report
Thu 26 May 2016
SRR1043312_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043312_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences612680
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT498688.139322321603446No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT52020.8490566037735849No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT38590.6298557158712542Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC24850.40559509042240643No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT23840.38911013906117387No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG18700.3052164261931188No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT14620.2386237513873474No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC12470.20353202324214925No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT12290.20059411111836523No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC10510.17154142456094534No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC10320.1684402950969511No Hit
CGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG9640.1573415159626559No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG8950.1460795194881504No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT8180.13351178429196317No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC8030.13106352418880982No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC7970.13008422014754847No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT7790.12714630802376445No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT7420.12110726643598616No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC7080.11555787686883855No Hit
GGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC6830.11147744336358295No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC6410.10462231507475353No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCCA351.0185249E-746.00000412
ATTTAGG1100.046.0000041
GGCACGG351.0185249E-746.0000041
TCGATGG453.092282E-1046.0000041
GCTTAGG1100.046.0000041
TCCACGG551.8189894E-1246.0000041
GCGATTT453.092282E-1046.0000048
GCTAGTC700.046.00000426
AGAATGG453.092282E-1046.0000041
GCGTAGG1100.046.0000041
AGCACGG551.8189894E-1246.0000041
TATTTGG301.8599694E-646.01
TGTCAGA206.3091354E-446.043
CGGGTAG206.3091354E-446.04
CAGCGCC253.415275E-546.019
TGATAGA206.3091354E-446.030
GTACCGG301.8599694E-646.01
AGATCTA206.3091354E-446.034
ACCGGGT206.3091354E-446.03
ACACGAC206.3091354E-446.025
Produced by FastQC (version 0.11.5)