##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043312_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	612680
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.182431285499774	33.0	31.0	34.0	30.0	34.0
2	32.51519879872038	34.0	31.0	34.0	31.0	34.0
3	32.55928380231116	34.0	31.0	34.0	31.0	34.0
4	36.105818698178496	37.0	35.0	37.0	35.0	37.0
5	35.985114578572826	37.0	35.0	37.0	35.0	37.0
6	36.26027453156623	37.0	36.0	37.0	35.0	37.0
7	36.17643631259385	37.0	37.0	37.0	35.0	37.0
8	36.14978944963113	37.0	37.0	37.0	35.0	37.0
9	37.721784618397855	39.0	38.0	39.0	35.0	39.0
10	37.572083306130445	39.0	37.0	39.0	35.0	39.0
11	37.24576287784814	39.0	37.0	39.0	34.0	39.0
12	37.2198015277143	39.0	37.0	39.0	34.0	39.0
13	37.22603153359013	39.0	37.0	39.0	34.0	39.0
14	38.62887804400339	40.0	38.0	41.0	34.0	41.0
15	38.73639746686688	40.0	38.0	41.0	35.0	41.0
16	38.75589377815499	40.0	38.0	41.0	35.0	41.0
17	38.732494940262455	40.0	38.0	41.0	35.0	41.0
18	38.429770842854346	40.0	38.0	41.0	35.0	41.0
19	38.13016093229745	40.0	37.0	41.0	34.0	41.0
20	37.78410915975713	39.0	35.0	41.0	34.0	41.0
21	37.721998433113534	39.0	35.0	41.0	34.0	41.0
22	37.67649833518313	39.0	35.0	41.0	34.0	41.0
23	37.68115655807273	39.0	35.0	41.0	34.0	41.0
24	37.55370176927597	39.0	35.0	41.0	34.0	41.0
25	37.47860383887184	39.0	35.0	41.0	34.0	41.0
26	37.43258307762617	39.0	35.0	41.0	34.0	41.0
27	37.43226480381276	39.0	35.0	41.0	34.0	41.0
28	37.388127570673106	39.0	35.0	41.0	33.0	41.0
29	37.310183129855716	39.0	35.0	41.0	33.0	41.0
30	37.25434158124959	39.0	35.0	41.0	33.0	41.0
31	37.02771267219429	39.0	35.0	41.0	33.0	41.0
32	36.88187961088986	39.0	35.0	41.0	33.0	41.0
33	36.66448553894366	39.0	35.0	41.0	32.0	41.0
34	36.39859469870079	39.0	35.0	41.0	31.0	41.0
35	36.25764510021545	39.0	35.0	41.0	31.0	41.0
36	36.25880231115754	39.0	35.0	41.0	31.0	41.0
37	36.246929881830646	39.0	35.0	41.0	31.0	41.0
38	36.190938173271526	39.0	35.0	41.0	31.0	41.0
39	36.175933603186	39.0	35.0	41.0	30.0	41.0
40	36.166702030423714	39.0	35.0	41.0	31.0	41.0
41	36.136324019063785	39.0	35.0	41.0	31.0	41.0
42	36.01829503166417	39.0	35.0	41.0	30.0	41.0
43	35.84746523470653	38.0	35.0	41.0	29.0	41.0
44	35.7066739570412	38.0	35.0	40.0	29.0	41.0
45	35.64059868120389	38.0	35.0	40.0	28.0	41.0
46	35.6174658875759	38.0	35.0	41.0	28.0	41.0
47	35.49582326826402	38.0	35.0	40.0	28.0	41.0
48	35.443561075928706	38.0	35.0	40.0	28.0	41.0
49	35.36528693608409	38.0	35.0	40.0	28.0	41.0
50	35.17874583795783	37.0	35.0	40.0	27.0	41.0
51	35.06811549258993	37.0	35.0	40.0	27.0	41.0
52	34.6727492328785	36.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	14.0
16	15.0
17	49.0
18	101.0
19	233.0
20	422.0
21	748.0
22	1216.0
23	1959.0
24	3545.0
25	6198.0
26	8453.0
27	8978.0
28	8631.0
29	8647.0
30	9692.0
31	11656.0
32	14377.0
33	18979.0
34	32836.0
35	63990.0
36	70482.0
37	47303.0
38	77594.0
39	216242.0
40	316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.837370242214533	19.373571848273162	25.762877848142583	32.02618006136972
2	35.20614350068551	19.957237056864923	25.33002546190507	19.506593980544494
3	20.588072076777436	21.123098517986552	39.952503753998826	18.336325651237185
4	22.642488738003525	23.479467258601556	36.39485538943658	17.483188613958347
5	19.38809819155187	28.343017562185807	33.16641640007835	19.10246784618398
6	69.96180714239081	1.5732519422863485	24.12499183913299	4.339949076189854
7	69.75370503362277	1.2992100280733825	20.335738068812432	8.611346869491415
8	56.68897303649539	2.8220278122347717	29.801364496964155	10.687634654305674
9	32.04756153293726	17.300711627603317	31.628256185937193	19.02347065352223
10	25.40853300254619	17.167689495331985	45.406900829144085	12.016876672977737
11	22.38297316706927	14.326075602271985	41.98325390089443	21.307697329764313
12	16.147907553698502	19.179832865443625	45.88202650649605	18.79023307436182
13	11.067931056995494	22.834269112750537	45.129104916106286	20.96869491414768
14	9.2927792648691	21.855454723509826	49.04730038519292	19.804465626428154
15	10.21152967291245	17.600541881569498	51.63347914082392	20.55444930469413
16	10.631487889273357	24.040282039563884	42.25794868446824	23.07028138669452
17	12.531664164000784	21.851700724684992	43.99768231376902	21.61895279754521
18	14.203825814454527	29.244303714826664	40.85101521185611	15.7008552588627
19	14.293105699549521	23.80459620030032	37.33906770255272	24.56323039759744
20	11.481197362407784	31.29937324541359	35.85150486387674	21.367924528301888
21	12.430632630410656	24.201377554351374	36.98994581184305	26.37804400339492
22	10.401677874257361	27.726219233531367	33.3717438140628	28.500359078148463
23	10.173336815303259	23.554220800417834	44.112097669256386	22.16034471502252
24	15.538454005353527	21.217764575308482	40.79535809884442	22.448423320493568
25	11.62466540445257	24.54103283932885	40.42975125677352	23.40455049944506
26	14.798753019520793	21.22478292093752	37.41986028595678	26.556603773584907
27	21.53016256447085	21.251876999412417	39.349742116602464	17.868218319514266
28	15.83061304432983	21.350297055559185	35.22523992949011	27.593849970620877
29	17.5654501534243	20.66968074688255	32.660605862766865	29.104263236926293
30	26.199647450545143	18.624730691388653	29.114872364039957	26.060749494026247
31	20.258373049552787	17.38411568845074	33.410426323692626	28.947084938303846
32	19.05448194816217	18.76052751844356	29.269275967878826	32.915714565515444
33	22.569040934908923	16.46340667232487	35.58186981784945	25.385682574916757
34	17.283900241561664	14.998204609257687	36.773356401384085	30.944538747796567
35	21.140073121368413	20.0510870274858	33.884899131683746	24.923940719462035
36	20.828817653587517	17.096363517660116	34.97959783247372	27.095220996278645
37	19.159593915257556	22.03466736306065	35.380133185349614	23.42560553633218
38	14.936508454658224	22.636286479075537	35.15244499575635	27.274760070509892
39	20.31957955213162	20.25527192008879	38.76819873343344	20.65694979434615
40	14.909577593523535	16.126362864790757	43.1704968335836	25.793562708102108
41	18.98021805836652	20.383723966834236	34.24054971600183	26.395508258797413
42	20.177090814128093	15.96200300319906	40.0128941698766	23.84801201279624
43	22.964516550238297	19.02232813214076	37.37122151857413	20.64193379904681
44	16.07266435986159	21.484135274531564	37.05066266240125	25.392537703205587
45	20.412776653391656	19.740321211725533	35.42028465104133	24.426617483841483
46	23.65851668081217	16.739407194620355	34.68890774955931	24.91316837500816
47	17.376770908141282	16.119181301821506	38.919011555787684	27.58503623424953
48	18.335672781876347	13.178984135274533	35.17529542338578	33.31004765946334
49	20.091401710517726	14.212149898805249	40.23242149245936	25.464026898217668
50	18.536430110334923	13.72380361689626	40.95694326565255	26.782823007116274
51	15.275510870274859	12.954886727165892	40.64209701638702	31.127505386172228
52	14.101162107462297	13.09688581314879	48.88930599986943	23.91264607951949
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	207.0
1	1105.0
2	2003.0
3	27465.5
4	52928.0
5	28364.5
6	3801.0
7	3684.5
8	3568.0
9	3815.0
10	4062.0
11	4322.5
12	4583.0
13	4481.0
14	4088.0
15	3797.0
16	3520.0
17	3243.0
18	2989.0
19	2735.0
20	2587.0
21	2439.0
22	2351.0
23	2263.0
24	2228.0
25	2193.0
26	2168.0
27	2143.0
28	2297.5
29	2452.0
30	2631.0
31	2810.0
32	3320.5
33	3831.0
34	4441.5
35	5052.0
36	6042.0
37	7032.0
38	7156.0
39	8763.5
40	10247.0
41	13010.5
42	15774.0
43	20619.0
44	25464.0
45	31040.0
46	36616.0
47	47276.5
48	57937.0
49	75264.5
50	92592.0
51	95959.0
52	99326.0
53	83936.5
54	68547.0
55	52470.0
56	36393.0
57	27501.0
58	18609.0
59	14236.5
60	9864.0
61	7941.5
62	6019.0
63	4864.0
64	3003.5
65	2298.0
66	1946.0
67	1594.0
68	1540.0
69	1486.0
70	1275.0
71	1064.0
72	968.5
73	873.0
74	746.5
75	620.0
76	456.5
77	293.0
78	278.0
79	263.0
80	223.0
81	183.0
82	116.5
83	50.0
84	42.0
85	34.0
86	21.0
87	8.0
88	7.5
89	5.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	612680.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.92510191094589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.42948739135569	23.017394827571373
2	11.295483161827137	6.98627935825755
3	4.371630855964426	4.055793892132064
4	2.1836044420887197	2.7011275961915118
5	1.314768193525316	2.0329670187020312
6	0.8670634618719067	1.6088415552987758
7	0.6141143723431746	1.329408468478247
8	0.4350807457761622	1.0763933122094524
9	0.40001167018656025	1.1133361499478347
>10	3.455631117388995	23.047480117431885
>50	0.41555418170772807	8.669305614772242
>100	0.20380682797129981	10.885089501318216
>500	0.007940525765115575	1.7405403976179523
>1k	0.004764315459069346	2.720658471408272
>5k	5.293683843410384E-4	0.8516075196020116
>10k+	5.293683843410384E-4	8.163776199060575
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49868	8.139322321603446	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	5202	0.8490566037735849	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	3859	0.6298557158712542	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	2485	0.40559509042240643	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	2384	0.38911013906117387	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	1870	0.3052164261931188	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1462	0.2386237513873474	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1247	0.20353202324214925	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	1229	0.20059411111836523	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1051	0.17154142456094534	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1032	0.1684402950969511	No Hit
CGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	964	0.1573415159626559	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	895	0.1460795194881504	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	818	0.13351178429196317	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	803	0.13106352418880982	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	797	0.13008422014754847	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	779	0.12714630802376445	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	742	0.12110726643598616	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	708	0.11555787686883855	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	683	0.11147744336358295	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	641	0.10462231507475353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6497682313769014	0.0
2	0.0	0.0	0.0	2.2912450218711236	0.0
3	0.0	0.0	0.0	3.9829927531500946	0.0
4	0.0	0.0	0.0	5.178396552849775	0.0
5	0.0	0.0	0.0	10.864725468433766	0.0
6	0.0	0.0	0.0	12.615721094209048	0.0
7	0.0	0.0	0.0	18.217503427564143	0.0
8	0.0	0.0	0.0	24.87807664686296	0.0
9	0.0	0.0	0.0	30.1756218580662	0.0
10	0.0	0.0	0.0	32.79803486322387	0.0
11	0.0	0.0	0.0	35.15456682117908	0.0
12	0.0	0.0	0.0	36.85839263563361	0.0
13	0.0	0.0	0.0	37.518280342103544	0.0
14	0.0	0.0	0.0	37.979042893517004	0.0
15	0.0	0.0	0.0	39.35904550499445	0.0
16	0.0	0.0	0.0	40.98044656264282	0.0
17	0.0	0.0	0.0	43.07403538551936	0.0
18	0.0	0.0	0.0	43.99213292420187	0.0
19	0.0	0.0	0.0	45.127472742704185	0.0
20	0.0	0.0	0.0	46.18071423908076	0.0
21	0.0	0.0	0.0	47.11872429326892	0.0
22	6.528693608408957E-4	0.0	0.0	48.070281386694525	0.0
23	8.160867010511197E-4	0.0	0.0	48.74567474048443	0.0
24	0.0014689560618920154	0.0	0.0	49.34207090161259	0.0
25	0.0014689560618920154	0.0	0.0	49.8914604687602	0.0
26	0.0014689560618920154	0.0	0.0	50.4741463733107	0.0
27	0.0014689560618920154	0.0	0.0	50.99268786315858	0.0
28	0.0014689560618920154	0.0	0.0	51.52542926160475	0.0
29	0.0014689560618920154	0.0	0.0	52.167689495331985	0.0
30	0.0014689560618920154	0.0	0.0	52.7347065352223	0.0
31	0.0014689560618920154	0.0	0.0	53.212117255337205	0.0
32	0.0014689560618920154	0.0	0.0	53.678265978977606	0.0
33	0.0014689560618920154	0.0	0.0	54.13364235816413	0.0
34	0.0014689560618920154	0.0	0.0	54.67813540510544	0.0
35	0.0014689560618920154	0.0	0.0	55.104948749755174	0.0
36	0.0014689560618920154	0.0	0.0	55.533067833126594	0.0
37	0.0014689560618920154	0.0	0.0	55.98697525625123	0.0
38	0.0014689560618920154	0.0	0.0	56.44039302735523	0.0
39	0.0014689560618920154	0.0	0.0	56.88075341124241	0.0
40	0.0016321734021022393	0.0	0.0	57.31376901482013	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCCA	35	1.0185249E-7	46.000004	12
ATTTAGG	110	0.0	46.000004	1
GGCACGG	35	1.0185249E-7	46.000004	1
TCGATGG	45	3.092282E-10	46.000004	1
GCTTAGG	110	0.0	46.000004	1
TCCACGG	55	1.8189894E-12	46.000004	1
GCGATTT	45	3.092282E-10	46.000004	8
GCTAGTC	70	0.0	46.000004	26
AGAATGG	45	3.092282E-10	46.000004	1
GCGTAGG	110	0.0	46.000004	1
AGCACGG	55	1.8189894E-12	46.000004	1
TATTTGG	30	1.8599694E-6	46.0	1
TGTCAGA	20	6.3091354E-4	46.0	43
CGGGTAG	20	6.3091354E-4	46.0	4
CAGCGCC	25	3.415275E-5	46.0	19
TGATAGA	20	6.3091354E-4	46.0	30
GTACCGG	30	1.8599694E-6	46.0	1
AGATCTA	20	6.3091354E-4	46.0	34
ACCGGGT	20	6.3091354E-4	46.0	3
ACACGAC	20	6.3091354E-4	46.0	25
>>END_MODULE