SRR1043311_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043311_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	588630
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45439	7.719450248882999	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	4908	0.8338005198511798	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3709	0.630107198070095	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	2374	0.4033093794064183	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	2339	0.39736336917928067	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1756	0.2983198273958174	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1389	0.23597166301411754	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1287	0.2186432903521737	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	1226	0.20828024395630534	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1073	0.18228768496338957	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1066	0.18109848291796204	No Hit
CGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	962	0.1634303382430389	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	826	0.14032584136044712	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	795	0.13505937515926814	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	772	0.1311519970100063	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC	743	0.12622530282180658	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	741	0.12588553080882728	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	715	0.12146849464009649	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	701	0.11909009054924145	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	688	0.11688157246487606	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	641	0.10889693015986274	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	629	0.10685829808198698	No Hit
ATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	608	0.10329069194570442	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	55	1.8189894E-12	45.000004	1
AGTAAGG	110	0.0	45.000004	1
CTAGCGG	105	0.0	45.000004	1
TAATATG	25	3.8884777E-5	45.0	40
ATGGGCG	85	0.0	45.0	4
TCACCGG	35	1.2105738E-7	45.0	1
TTCAGGT	20	7.0304796E-4	45.0	9
TTTACGG	70	0.0	45.0	1
GCTGGTC	25	3.8884777E-5	45.0	8
CGCGGCT	25	3.8884777E-5	45.0	3
ACCGCTT	20	7.0304796E-4	45.0	28
ATTACGG	35	1.2105738E-7	45.0	1
ACATTAG	25	3.8884777E-5	45.0	24
CACGCGG	20	7.0304796E-4	45.0	1
CGATTAG	85	0.0	45.0	41
AAGCGGT	25	3.8884777E-5	45.0	2
CCGCTTG	20	7.0304796E-4	45.0	29
ACGGGTC	20	7.0304796E-4	45.0	4
TCGGCAG	45	3.8380676E-10	45.0	18
TACATTA	25	3.8884777E-5	45.0	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content