##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043311_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	588630
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094565346652395	33.0	31.0	34.0	30.0	34.0
2	32.41216044034453	34.0	31.0	34.0	30.0	34.0
3	32.44180554847697	34.0	31.0	34.0	31.0	34.0
4	36.04276030783345	37.0	35.0	37.0	35.0	37.0
5	35.98574146747532	37.0	35.0	37.0	35.0	37.0
6	36.24915651597778	37.0	37.0	37.0	35.0	37.0
7	36.17545656864244	37.0	37.0	37.0	35.0	37.0
8	36.07785026247388	37.0	37.0	37.0	35.0	37.0
9	37.64665069738206	39.0	38.0	39.0	35.0	39.0
10	37.49561184445237	39.0	37.0	39.0	35.0	39.0
11	37.222397771095594	39.0	37.0	39.0	34.0	39.0
12	37.201968978815216	39.0	37.0	39.0	34.0	39.0
13	37.22570545164195	39.0	37.0	39.0	34.0	39.0
14	38.6239946995566	40.0	38.0	41.0	35.0	41.0
15	38.742712739751624	40.0	38.0	41.0	35.0	41.0
16	38.79341351952839	40.0	38.0	41.0	35.0	41.0
17	38.74556172808046	40.0	38.0	41.0	35.0	41.0
18	38.47956441227936	40.0	38.0	41.0	35.0	41.0
19	38.19723765353448	40.0	37.0	41.0	35.0	41.0
20	37.88036797309006	40.0	35.0	41.0	34.0	41.0
21	37.8252042879228	40.0	35.0	41.0	34.0	41.0
22	37.79040653721353	40.0	35.0	41.0	34.0	41.0
23	37.759414233049625	39.0	35.0	41.0	34.0	41.0
24	37.64946400964953	39.0	35.0	41.0	34.0	41.0
25	37.640900056062385	39.0	35.0	41.0	34.0	41.0
26	37.621043779623875	39.0	35.0	41.0	34.0	41.0
27	37.58933455651258	39.0	35.0	41.0	34.0	41.0
28	37.52189320965632	39.0	35.0	41.0	34.0	41.0
29	37.47121961164059	39.0	35.0	41.0	34.0	41.0
30	37.36538402731767	39.0	35.0	41.0	33.0	41.0
31	37.14006761463058	39.0	35.0	41.0	33.0	41.0
32	36.9968299271189	39.0	35.0	41.0	33.0	41.0
33	36.75775444676622	39.0	35.0	41.0	32.0	41.0
34	36.44673733924537	39.0	35.0	41.0	31.0	41.0
35	36.29614528651275	39.0	35.0	41.0	31.0	41.0
36	36.2832696260809	39.0	35.0	41.0	31.0	41.0
37	36.21180198087084	39.0	35.0	41.0	31.0	41.0
38	36.15868372322172	39.0	35.0	41.0	30.0	41.0
39	36.09427823930143	39.0	35.0	41.0	30.0	41.0
40	36.069124917180574	39.0	35.0	41.0	30.0	41.0
41	35.98751337852301	39.0	35.0	41.0	30.0	41.0
42	35.87311893719314	38.0	35.0	41.0	29.0	41.0
43	35.68348368244908	38.0	35.0	40.0	28.0	41.0
44	35.58394577238673	38.0	35.0	40.0	28.0	41.0
45	35.55015204797581	38.0	35.0	40.0	28.0	41.0
46	35.46522943105177	38.0	35.0	40.0	28.0	41.0
47	35.280101252059865	38.0	35.0	40.0	27.0	41.0
48	35.266073764504014	38.0	35.0	40.0	27.0	41.0
49	35.16670064386796	38.0	35.0	40.0	27.0	41.0
50	35.0116728675059	37.0	35.0	40.0	26.0	41.0
51	34.52738562424613	36.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	1.0
14	2.0
15	10.0
16	13.0
17	43.0
18	77.0
19	223.0
20	373.0
21	642.0
22	994.0
23	1576.0
24	2920.0
25	5208.0
26	7798.0
27	8955.0
28	8646.0
29	8674.0
30	9575.0
31	11226.0
32	13982.0
33	18432.0
34	32248.0
35	61331.0
36	64808.0
37	46431.0
38	80053.0
39	204347.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.15410359648676	19.55557820702309	25.878225710548218	31.412092485941933
2	34.726908244567895	19.970949492890274	25.526561677114657	19.77558058542718
3	20.778247795729065	21.191410563511884	39.5717173776396	18.458624263119447
4	22.86088034928563	23.666819564072508	35.89130693304792	17.580993153593937
5	19.510388529296844	28.534563308020317	32.66381258175764	19.291235580925196
6	70.53751932453324	1.5259161102899954	23.58391519290556	4.352649372271206
7	70.42250649813975	1.2202911846151232	19.66940183137115	8.687800485873979
8	57.07082547610553	2.701017617178873	29.2929344409901	10.935222465725499
9	32.1526255882303	17.313932351392218	31.131780575234018	19.401661485143467
10	25.71853286444796	17.162224147596962	45.01860251771062	12.100640470244466
11	22.637140478738765	14.200431510456484	41.73725430236311	21.425173708441637
12	16.260299339143437	19.123558092519918	45.517217946757725	19.09892462157892
13	11.19327930958327	22.728029492210727	44.9073271834599	21.171364014746104
14	9.456025007220155	21.847340434568405	48.56735130727282	20.12928325093862
15	10.235971663014118	17.435910504051783	51.30200635373664	21.02611147919746
16	10.76703531930075	23.92929344409901	41.9934424001495	23.31022883645074
17	12.69303297487386	21.71024242733126	43.64660312930024	21.95012146849464
18	14.306950036525492	29.29225489696414	40.629767426057114	15.771027640453255
19	14.41210947454258	23.68941440293563	37.13640147461054	24.762074647911252
20	11.494147427076431	31.37539710854017	35.417155088935324	21.713300375448075
21	12.589402510915177	24.184971883865927	36.520904473098554	26.704721132120348
22	10.588145354467153	27.637904965767966	32.85663319912339	28.917316480641492
23	10.324991930414692	23.632502590761597	43.486740397193486	22.555765081630227
24	15.595535395749453	21.3026858977626	40.33416577476513	22.767612931722812
25	11.726721369960757	24.439631007593903	40.06710497256341	23.76654264988193
26	14.869952262032177	21.032567147444066	37.120771962013485	26.97670862851027
27	21.77479910979733	21.103579498156737	38.92852895706981	18.19309243497613
28	16.14834446086676	21.324261420586787	34.702614545639875	27.82477957290658
29	17.702631534240524	20.52290912797513	32.22329816692999	29.551161170854357
30	26.570681073000017	18.49650884256664	28.489713402307054	26.443096682126292
31	20.48315580245655	17.22100470584238	32.9121859232455	29.383653568455564
32	19.122029118461512	18.502284966787286	28.717020878990198	33.658665035761004
33	22.874131457791822	16.223094303722203	35.14312896046753	25.75964527801845
34	17.672391825085366	14.646552163498292	36.50425564446256	31.17680036695377
35	21.230484345004502	19.753835176596503	33.719823998097276	25.295856480301715
36	21.215194604420436	16.73241255117816	34.52695241492958	27.525440429471825
37	19.658189354942834	21.703786759084654	34.96661739972479	23.67140648624773
38	15.159098245077555	22.496814637378318	34.70652192378913	27.63756519375499
39	20.462939367684285	20.14865025567844	38.4791804699047	20.90922990673258
40	14.992779844724192	15.86990129623023	43.20727791651802	25.930040942527565
41	19.194740329239078	20.265361942136824	33.88223502030138	26.657662708322714
42	20.496746682975722	15.699675517727604	39.63780303416408	24.165774765132596
43	23.29748738596402	18.716511220970727	37.078640232404055	20.907361160661196
44	16.313813431187675	21.25070077977677	36.77046701663184	25.66501877240372
45	20.566909603655947	19.50631126514109	35.30655929871057	24.6202198324924
46	24.02442960773321	16.43052511764606	34.5529449739225	24.99210030069823
47	17.587109049827564	15.757776531947062	38.80757012044918	27.847544297776196
48	18.53303433396191	12.86037069126616	34.83580517472776	33.77078980004417
49	20.41299288177633	13.732055790564532	39.973158010974636	25.881793316684504
50	18.82642746716953	13.38327981924129	40.847221514363866	26.943071199225322
51	15.583303603282198	12.73635390652872	40.117221344477855	31.563121145711225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	968.0
2	1784.0
3	25033.0
4	48282.0
5	26113.0
6	3944.0
7	3677.0
8	3410.0
9	3683.0
10	3956.0
11	4028.0
12	4100.0
13	4050.5
14	4001.0
15	3674.5
16	3348.0
17	3042.5
18	2737.0
19	2517.0
20	2297.0
21	2169.0
22	2041.0
23	1976.5
24	1912.0
25	1864.5
26	1931.0
27	2045.0
28	2247.5
29	2450.0
30	2685.5
31	2921.0
32	3434.5
33	3948.0
34	4510.0
35	5072.0
36	5974.5
37	6877.0
38	7396.5
39	7916.0
40	10286.0
41	12656.0
42	16234.5
43	19813.0
44	23536.0
45	27259.0
46	31501.0
47	35743.0
48	51318.0
49	66893.0
50	84039.5
51	101186.0
52	98790.5
53	96395.0
54	74614.0
55	52833.0
56	39503.0
57	26173.0
58	19849.5
59	13526.0
60	10249.0
61	6972.0
62	5514.5
63	4057.0
64	3453.0
65	2849.0
66	2395.0
67	1941.0
68	1688.5
69	1436.0
70	1314.0
71	1192.0
72	1096.5
73	1001.0
74	834.0
75	530.0
76	393.0
77	338.0
78	283.0
79	251.0
80	219.0
81	141.0
82	63.0
83	54.0
84	45.0
85	26.5
86	8.0
87	6.5
88	5.0
89	6.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	588630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.71408079292774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0394851921816	22.74054730059443
2	11.354632276632733	6.974941862369656
3	4.449089746157927	4.099491057554428
4	2.223728144480618	2.7319906356433994
5	1.3423987551240497	2.061527191060284
6	0.8465381888358773	1.560038539572231
7	0.6082682421128044	1.3077679952417363
8	0.4656412016508812	1.14413931904169
9	0.3951671875987044	1.0923477233958576
>10	3.6546930365528594	24.03410268894058
>50	0.40147382773641094	8.345395056017894
>100	0.2049247493452886	10.797854917119292
>500	0.00781729289055597	1.6937408103662603
>1k	0.005583780636111406	3.6232950689153482
>5k	0.0	0.0
>10k+	5.583780636111407E-4	7.79281983416692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45439	7.719450248882999	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	4908	0.8338005198511798	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	3709	0.630107198070095	No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	2374	0.4033093794064183	No Hit
GCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	2339	0.39736336917928067	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCC	1756	0.2983198273958174	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1389	0.23597166301411754	No Hit
ACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1287	0.2186432903521737	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	1226	0.20828024395630534	No Hit
AGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1073	0.18228768496338957	No Hit
GCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	1066	0.18109848291796204	No Hit
CGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	962	0.1634303382430389	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCT	826	0.14032584136044712	No Hit
TCCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	795	0.13505937515926814	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	772	0.1311519970100063	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTC	743	0.12622530282180658	No Hit
TGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCTT	741	0.12588553080882728	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCT	715	0.12146849464009649	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTT	701	0.11909009054924145	No Hit
TGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	688	0.11688157246487606	No Hit
GGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	641	0.10889693015986274	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	629	0.10685829808198698	No Hit
ATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTG	608	0.10329069194570442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6518526069007696	0.0
2	0.0	0.0	0.0	2.302804817967144	0.0
3	0.0	0.0	0.0	4.089835720231725	0.0
4	0.0	0.0	0.0	5.3023121485483244	0.0
5	0.0	0.0	0.0	11.047517116015154	0.0
6	0.0	0.0	0.0	12.756910113313966	0.0
7	0.0	0.0	0.0	18.368075021660466	0.0
8	0.0	0.0	0.0	25.206666326894652	0.0
9	0.0	0.0	0.0	30.657628731121417	0.0
10	0.0	0.0	0.0	33.35728726024837	0.0
11	0.0	0.0	0.0	35.79226339126446	0.0
12	0.0	0.0	0.0	37.54956424239335	0.0
13	0.0	0.0	0.0	38.19479129504103	0.0
14	0.0	0.0	0.0	38.667414165095224	0.0
15	0.0	0.0	0.0	40.120619064607645	0.0
16	0.0	0.0	0.0	41.77615819784924	0.0
17	0.0	0.0	0.0	43.90550940319046	0.0
18	0.0	0.0	0.0	44.832237568591474	0.0
19	0.0	0.0	0.0	45.981006744474456	0.0
20	0.0	0.0	0.0	47.04228462701527	0.0
21	0.0	0.0	0.0	47.99874284355197	0.0
22	8.494300324482272E-4	0.0	0.0	48.971170344698706	0.0
23	8.494300324482272E-4	0.0	0.0	49.690467696175865	0.0
24	0.0018687460713860998	0.0	0.0	50.304775495642424	0.0
25	0.0018687460713860998	0.0	0.0	50.85163855053259	0.0
26	0.0018687460713860998	0.0	0.0	51.44114299305166	0.0
27	0.0018687460713860998	0.0	0.0	51.9638822350203	0.0
28	0.002038632077875745	0.0	0.0	52.507687341793655	0.0
29	0.002038632077875745	0.0	0.0	53.18060581349914	0.0
30	0.002038632077875745	0.0	0.0	53.7867590846542	0.0
31	0.002038632077875745	0.0	0.0	54.26991488711075	0.0
32	0.002038632077875745	0.0	0.0	54.74542581927527	0.0
33	0.002038632077875745	0.0	0.0	55.20632655488168	0.0
34	0.002038632077875745	0.0	0.0	55.73433226305149	0.0
35	0.002038632077875745	0.0	0.0	56.16108591135348	0.0
36	0.002038632077875745	0.0	0.0	56.58529126955813	0.0
37	0.002038632077875745	0.0	0.0	57.02597557039227	0.0
38	0.002038632077875745	0.0	0.0	57.458165570901926	0.0
39	0.002038632077875745	0.0	0.0	57.9178771044629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGG	55	1.8189894E-12	45.000004	1
AGTAAGG	110	0.0	45.000004	1
CTAGCGG	105	0.0	45.000004	1
TAATATG	25	3.8884777E-5	45.0	40
ATGGGCG	85	0.0	45.0	4
TCACCGG	35	1.2105738E-7	45.0	1
TTCAGGT	20	7.0304796E-4	45.0	9
TTTACGG	70	0.0	45.0	1
GCTGGTC	25	3.8884777E-5	45.0	8
CGCGGCT	25	3.8884777E-5	45.0	3
ACCGCTT	20	7.0304796E-4	45.0	28
ATTACGG	35	1.2105738E-7	45.0	1
ACATTAG	25	3.8884777E-5	45.0	24
CACGCGG	20	7.0304796E-4	45.0	1
CGATTAG	85	0.0	45.0	41
AAGCGGT	25	3.8884777E-5	45.0	2
CCGCTTG	20	7.0304796E-4	45.0	29
ACGGGTC	20	7.0304796E-4	45.0	4
TCGGCAG	45	3.8380676E-10	45.0	18
TACATTA	25	3.8884777E-5	45.0	23
>>END_MODULE