FastQCFastQC Report
Thu 26 May 2016
SRR1043309_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043309_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1629364
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT697364.279952177659504No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC76630.47030620536602014No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT65580.4024883328709853Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC45600.2798637996175195No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG44860.27532215023776146No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC33000.20253301288109962No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC31470.19314284592024863No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC25800.1583439918888597No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT24460.15011992409308172No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC22200.13624948139273974No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT21420.13146233745191374No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG18240.11194551984700779No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG17030.1045193093747008No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT16450.1009596382392148No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC16340.1002845281962778No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACG207.0341554E-445.016
TTACGAG207.0341554E-445.021
ACGTAGG900.045.01
TTCGATC207.0341554E-445.016
ATCGATT207.0341554E-445.013
GTACGTC253.8915263E-545.033
GCGATAA650.044.9999968
CGTTTTT195900.044.4027521
CTAGCGG3650.042.534251
TCAACGG1300.041.538461
ACGGTCT3150.041.42857429
ATACCGG603.6379788E-1241.2500041
TACGCGG1800.041.251
TAGTAGG3600.041.251
ATTGCGG3500.041.142861
CACGACG3300.040.9090925
TCACGTT2600.040.67307745
CCGCACT11800.040.61440733
CCCGCAC12150.040.55555332
TGTACGG1500.040.51
Produced by FastQC (version 0.11.5)