##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043309_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1629364
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27925497310607	33.0	31.0	34.0	31.0	34.0
2	32.58440164383158	34.0	31.0	34.0	31.0	34.0
3	32.67348057278791	34.0	31.0	34.0	31.0	34.0
4	36.18986303858438	37.0	37.0	37.0	35.0	37.0
5	36.15336290724479	37.0	37.0	37.0	35.0	37.0
6	36.35653052356625	37.0	37.0	37.0	35.0	37.0
7	36.30695228322217	37.0	37.0	37.0	35.0	37.0
8	36.2031080838904	37.0	37.0	37.0	35.0	37.0
9	37.74408235360546	39.0	38.0	39.0	35.0	39.0
10	37.54997532779661	39.0	37.0	39.0	35.0	39.0
11	37.29785364105258	39.0	37.0	39.0	35.0	39.0
12	37.33500678792461	39.0	37.0	39.0	35.0	39.0
13	37.33089966391795	39.0	37.0	39.0	34.0	39.0
14	38.71828885381044	40.0	38.0	41.0	35.0	41.0
15	38.80062650211985	40.0	38.0	41.0	35.0	41.0
16	38.76919706093911	41.0	38.0	41.0	35.0	41.0
17	38.719629254113876	40.0	38.0	41.0	34.0	41.0
18	38.50224320655176	40.0	38.0	41.0	34.0	41.0
19	38.27072219589975	40.0	37.0	41.0	34.0	41.0
20	38.001538637161495	40.0	35.0	41.0	34.0	41.0
21	37.918031207268605	40.0	35.0	41.0	34.0	41.0
22	37.88080502576465	40.0	35.0	41.0	34.0	41.0
23	37.851593627943174	40.0	35.0	41.0	34.0	41.0
24	37.75949020599449	40.0	35.0	41.0	34.0	41.0
25	37.73466149982447	40.0	35.0	41.0	34.0	41.0
26	37.71115662307501	40.0	35.0	41.0	34.0	41.0
27	37.66605743099762	40.0	35.0	41.0	34.0	41.0
28	37.59734043467267	39.0	35.0	41.0	34.0	41.0
29	37.52571985142669	39.0	35.0	41.0	34.0	41.0
30	37.43414853893912	40.0	35.0	41.0	33.0	41.0
31	37.25934168178504	40.0	35.0	41.0	33.0	41.0
32	37.01971812314498	40.0	35.0	41.0	33.0	41.0
33	36.642709670767246	39.0	35.0	41.0	31.0	41.0
34	36.311636319447345	39.0	35.0	41.0	30.0	41.0
35	36.140859869249596	39.0	35.0	41.0	30.0	41.0
36	36.053326328555194	39.0	35.0	41.0	30.0	41.0
37	35.90171257005801	39.0	35.0	41.0	29.0	41.0
38	35.83922868063858	39.0	35.0	41.0	29.0	41.0
39	35.73241031469948	39.0	35.0	41.0	28.0	41.0
40	35.63404678144356	39.0	35.0	41.0	27.0	41.0
41	35.535956974623225	38.0	35.0	40.0	27.0	41.0
42	35.45574776415828	38.0	35.0	40.0	26.0	41.0
43	35.35505632872704	38.0	35.0	40.0	26.0	41.0
44	35.28253539417834	38.0	35.0	40.0	25.0	41.0
45	35.246190538148625	38.0	35.0	40.0	25.0	41.0
46	35.16439113666437	38.0	35.0	40.0	24.0	41.0
47	35.02536327057674	38.0	35.0	40.0	24.0	41.0
48	34.95908771766162	38.0	35.0	40.0	24.0	41.0
49	34.88926538207546	38.0	35.0	40.0	24.0	41.0
50	34.80631031494497	37.0	35.0	40.0	24.0	41.0
51	34.40745407410499	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	8.0
15	20.0
16	48.0
17	144.0
18	257.0
19	567.0
20	1066.0
21	1759.0
22	2895.0
23	4725.0
24	8352.0
25	15929.0
26	25145.0
27	30279.0
28	30229.0
29	28432.0
30	28893.0
31	32082.0
32	36974.0
33	48276.0
34	96826.0
35	141238.0
36	135095.0
37	135761.0
38	237529.0
39	586749.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.631394826447618	17.65259328179584	24.92800871996681	29.78800317178973
2	33.77888550379166	19.79938184469523	24.80857561600723	21.61315703550588
3	23.056726428225982	20.991319312320638	36.16595186833636	19.786002391117023
4	22.660989195784367	23.8829997471406	33.60550497003739	19.85050608703764
5	20.79111849776968	30.56002219270832	30.557567247097637	18.09129206242436
6	76.65340586879297	1.9325945583675594	18.47690264422192	2.9370969286175463
7	77.02477776604859	1.634134545749139	16.334901225263355	5.0061864629389135
8	67.31798419506015	3.596188451444858	22.127897756425206	6.957929597069777
9	36.45864275876968	19.13267999047481	26.55373507699937	17.854942173756143
10	26.968375390643217	19.649814283364552	38.50391932066745	14.877891005324779
11	24.75493505441387	15.961319876958127	39.724702399218344	19.55904266940966
12	21.558841363869583	17.210150709111037	40.88061354000702	20.350394387012354
13	16.58389408382657	20.00596551783395	41.95459087103926	21.45554952730022
14	16.671658389408385	20.32394234805728	41.470843838454755	21.53355542407958
15	19.071490471128612	17.435760210732532	42.3688629428415	21.123886375297353
16	18.70288038768501	21.053859051752706	38.925003866539335	21.318256694022946
17	19.782135851780204	20.014373706550533	38.83196142789456	21.3715290137747
18	19.954043418168073	26.081833156986406	36.548555141760836	17.41556828308469
19	20.355181530953182	22.310791204420866	35.20293807890686	22.13108918571909
20	18.72215171072885	27.033615570247044	35.15531213405967	19.088920584964438
21	17.60355574322251	21.29689866721003	37.166710446530054	23.932835143037405
22	17.477064670632224	23.493461252365954	34.4676818685082	24.56179220849362
23	17.71261670197697	21.290884050463863	39.13999572839464	21.856503519164534
24	20.297428935461934	20.36806999540925	36.41641769426599	22.91808337486283
25	16.019072472449373	22.45551024817045	38.29641504292473	23.22900223645545
26	16.59414348175116	20.186097152017595	37.381886429306164	25.837872936925084
27	20.340942846411238	21.202812876680717	37.646897807979066	20.80934646892898
28	18.479112095271528	21.550126306951668	34.26091407444868	25.709847523328122
29	18.892279441548972	20.382922416353864	37.2175891943114	23.507208947785763
30	21.349679997839647	20.61215296275111	32.35820847889115	25.67995856051809
31	20.555443719144403	19.993199800658417	34.46577928565993	24.985577194537257
32	19.700324789304293	19.23523534336097	33.743595660638135	27.3208442066966
33	17.422319383514058	21.686866777466545	34.788604633464345	26.10220920555505
34	17.297178531009646	19.693573688874924	34.94713274627401	28.062115033841422
35	20.437360835270695	23.037455105182143	30.898743313341893	25.62644074620527
36	20.62792598829973	23.160202385716143	31.334005170115457	24.87786645586867
37	20.539793440876316	24.151693544229527	33.69468086934534	21.613832145548812
38	22.617720779396134	21.600943681092748	31.085319179753572	24.69601635975755
39	25.397639815289892	19.990315239565867	33.57236320429321	21.039681740851034
40	20.543107617450733	19.5851878401634	36.119737517215306	23.751967025170558
41	20.97634414409549	21.948441232284498	31.75490559506654	25.320309028553474
42	20.475351118595967	18.366061849899715	33.701922958896844	27.456664072607474
43	22.157602598314437	20.214083531979348	32.818694901814446	24.809618967891765
44	18.401904055815642	22.064621533309932	34.76485303468102	24.768621376193412
45	20.18149412899757	21.620337751417118	34.071883262426326	24.12628485715899
46	22.24076388087622	19.59991751382748	33.158029758850695	25.001288846445608
47	19.578191245172963	19.31004981084644	35.58652333057561	25.525235613404984
48	20.65020461971665	18.342126130195584	32.0800631412011	28.927606108886657
49	20.618842689540212	17.46423757981642	35.86749185571794	26.049427874925428
50	19.934526600563167	17.6454125658846	36.99339128641605	25.426669547136182
51	18.16144213324954	16.887448108587154	36.132257739829775	28.818852018333533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	1822.5
2	3399.0
3	47246.5
4	91094.0
5	53325.0
6	15556.0
7	14349.5
8	13143.0
9	13085.0
10	13027.0
11	12801.0
12	12575.0
13	11996.5
14	11418.0
15	10772.5
16	10127.0
17	9256.5
18	8386.0
19	7680.0
20	6974.0
21	6498.5
22	6023.0
23	5829.5
24	5636.0
25	5674.5
26	6594.0
27	7475.0
28	7964.5
29	8454.0
30	10234.5
31	12015.0
32	13273.0
33	14531.0
34	17084.5
35	19638.0
36	22762.5
37	25887.0
38	29731.5
39	33576.0
40	37284.0
41	40992.0
42	49291.5
43	57591.0
44	64841.0
45	72091.0
46	83667.5
47	95244.0
48	122092.5
49	148941.0
50	176063.0
51	203185.0
52	203426.5
53	203668.0
54	172878.0
55	142088.0
56	118589.5
57	95091.0
58	80195.0
59	65299.0
60	56853.5
61	48408.0
62	44313.5
63	40219.0
64	35404.0
65	30589.0
66	24659.5
67	18730.0
68	15319.5
69	11909.0
70	10382.0
71	8855.0
72	9011.0
73	9167.0
74	7779.5
75	4776.0
76	3160.0
77	2220.5
78	1281.0
79	969.0
80	657.0
81	520.5
82	384.0
83	364.5
84	345.0
85	239.0
86	133.0
87	77.5
88	22.0
89	16.5
90	11.0
91	10.5
92	10.0
93	9.0
94	8.0
95	4.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1629364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.59717006231847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41189810623983	24.66432025272935
2	11.734383136661378	7.884841316376286
3	4.394508465930992	4.429291448105452
4	2.1369493635700523	2.8718180472970505
5	1.2997655731878726	2.18342225017699
6	0.8700792762223221	1.753932084656419
7	0.6202379460409716	1.4586767826569136
8	0.491522533198774	1.3211012909872626
9	0.4148541762432882	1.2544133679277913
>10	4.22140549223877	29.719089643369028
>50	0.2714034402639625	6.126329929516246
>100	0.12341409335757678	7.239487158917159
>500	0.0049733983614013	1.1840023180622816
>1k	0.004052398664845503	2.7135139891321796
>5k	3.683998786223185E-4	0.8800803347879839
>10k+	1.8419993931115925E-4	4.315679785301656
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69736	4.279952177659504	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	7663	0.47030620536602014	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	6558	0.4024883328709853	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4560	0.2798637996175195	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	4486	0.27532215023776146	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3300	0.20253301288109962	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	3147	0.19314284592024863	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCC	2580	0.1583439918888597	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	2446	0.15011992409308172	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2220	0.13624948139273974	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	2142	0.13146233745191374	No Hit
GCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	1824	0.11194551984700779	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	1703	0.1045193093747008	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	1645	0.1009596382392148	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC	1634	0.1002845281962778	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4147630609243852	0.0
2	0.0	0.0	0.0	1.4020808118996124	0.0
3	0.0	0.0	0.0	2.3793946595113185	0.0
4	0.0	0.0	0.0	2.9890190282834284	0.0
5	0.0	0.0	0.0	5.974048769949502	0.0
6	0.0	0.0	0.0	6.88176490949843	0.0
7	0.0	0.0	0.0	9.607306900115628	0.0
8	0.0	0.0	0.0	12.797140479352679	0.0
9	0.0	0.0	0.0	15.690293881539054	0.0
10	0.0	0.0	0.0	17.334800572493318	0.0
11	0.0	0.0	0.0	18.787698758534006	0.0
12	0.0	0.0	0.0	19.881499775372475	0.0
13	0.0	0.0	0.0	20.416677918500717	0.0
14	0.0	0.0	0.0	20.808732732526313	0.0
15	0.0	0.0	0.0	21.87890489786199	0.0
16	0.0	0.0	0.0	23.091832150458707	0.0
17	0.0	0.0	0.0	24.443893445540713	0.0
18	0.0	0.0	0.0	25.269307533491595	0.0
19	0.0	0.0	0.0	26.161618889333507	0.0
20	0.0	0.0	0.0	27.164402797656017	0.0
21	0.0	0.0	0.0	28.183205226088216	0.0
22	0.0	0.0	0.0	29.15953709545565	0.0
23	0.0	0.0	0.0	29.97439491728061	0.0
24	6.137364026699988E-5	0.0	0.0	30.69406222305145	0.0
25	6.137364026699988E-5	0.0	0.0	31.377519081064758	0.0
26	6.137364026699988E-5	0.0	0.0	32.07521462362001	0.0
27	6.137364026699988E-5	0.0	0.0	32.76585219754456	0.0
28	1.2274728053399977E-4	0.0	0.0	33.459067464360324	0.0
29	1.2274728053399977E-4	0.0	0.0	34.21267439319882	0.0
30	1.2274728053399977E-4	0.0	0.0	34.89441278928465	0.0
31	1.2274728053399977E-4	0.0	0.0	35.55227683930663	0.0
32	1.2274728053399977E-4	0.0	0.0	36.22530017847455	0.0
33	1.2274728053399977E-4	0.0	0.0	36.88463719586293	0.0
34	1.2274728053399977E-4	0.0	0.0	37.58294647482085	0.0
35	1.2274728053399977E-4	0.0	0.0	38.1759385870806	0.0
36	1.2274728053399977E-4	0.0	0.0	38.806798235385095	0.0
37	1.2274728053399977E-4	0.0	0.0	39.51676850599375	0.0
38	1.2274728053399977E-4	0.0	0.0	40.17770123802907	0.0
39	1.2274728053399977E-4	0.0	0.0	40.94204855391429	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	20	7.0341554E-4	45.0	16
TTACGAG	20	7.0341554E-4	45.0	21
ACGTAGG	90	0.0	45.0	1
TTCGATC	20	7.0341554E-4	45.0	16
ATCGATT	20	7.0341554E-4	45.0	13
GTACGTC	25	3.8915263E-5	45.0	33
GCGATAA	65	0.0	44.999996	8
CGTTTTT	19590	0.0	44.402752	1
CTAGCGG	365	0.0	42.53425	1
TCAACGG	130	0.0	41.53846	1
ACGGTCT	315	0.0	41.428574	29
ATACCGG	60	3.6379788E-12	41.250004	1
TACGCGG	180	0.0	41.25	1
TAGTAGG	360	0.0	41.25	1
ATTGCGG	350	0.0	41.14286	1
CACGACG	330	0.0	40.90909	25
TCACGTT	260	0.0	40.673077	45
CCGCACT	1180	0.0	40.614407	33
CCCGCAC	1215	0.0	40.555553	32
TGTACGG	150	0.0	40.5	1
>>END_MODULE