FastQCFastQC Report
Thu 26 May 2016
SRR1043308_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043308_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2727253
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1347614.941272408537088No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC163200.5984043284579759No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT129390.4744334317351562TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT108550.3980195456747137TruSeq Adapter, Index 20 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA103360.3789894080233847No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC78420.2875420798877112TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT65270.23932506445129956TruSeq Adapter, Index 22 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT63200.23173500954990242No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC49480.18142797899571475No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG43650.16005115770337405No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43110.15807114338127046No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC41440.15194776575550564No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT38820.1423410296001141No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT31370.11502416534146265No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG31300.11476749681822698No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31190.11436416056742811No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT29960.1098541279448588TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT28980.10626076861955967No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTA206.3129864E-446.020
TACGATA301.8623505E-646.022
CGTTTTT331650.045.237151
CGGTCTA6100.042.98360430
TCACGAC6450.042.79069524
CACGACG6250.042.68825
AATGCGG3800.042.368421
ATGTTCG601.8189894E-1242.16666817
GCGCGAC22800.041.9649128
TACGCGG3400.041.9411771
CACTTAC43850.041.75142336
TCGGCGT16700.041.7305374
CATGCGG3650.041.5890431
TAGTAGG5300.041.2264181
CGACGGT6600.041.12121227
ACCCGCA45550.041.0515931
ACTTACT44250.041.0101737
CCGCACT45050.040.99666633
CGCACTT44850.040.97435834
GCACTTA44850.040.82051535
Produced by FastQC (version 0.11.5)