##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043308_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2727253
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36435160214326	33.0	31.0	34.0	31.0	34.0
2	32.695059094260785	34.0	31.0	34.0	31.0	34.0
3	32.81060943007488	34.0	31.0	34.0	31.0	34.0
4	36.239072062621254	37.0	37.0	37.0	35.0	37.0
5	36.18166484737573	37.0	37.0	37.0	35.0	37.0
6	36.337897510792	37.0	37.0	37.0	35.0	37.0
7	36.27149076378319	37.0	37.0	37.0	35.0	37.0
8	36.25047914513248	37.0	37.0	37.0	35.0	37.0
9	37.8350692070006	39.0	38.0	39.0	35.0	39.0
10	37.70103067078852	39.0	37.0	39.0	35.0	39.0
11	37.49046164767258	39.0	37.0	39.0	35.0	39.0
12	37.517882279348484	39.0	37.0	39.0	35.0	39.0
13	37.52275421458882	39.0	37.0	39.0	35.0	39.0
14	38.8891811650771	41.0	38.0	41.0	35.0	41.0
15	38.959020670249515	41.0	38.0	41.0	35.0	41.0
16	38.87680534222531	41.0	38.0	41.0	35.0	41.0
17	38.84535648141188	41.0	38.0	41.0	35.0	41.0
18	38.61004607933331	40.0	38.0	41.0	35.0	41.0
19	38.34833658630131	40.0	37.0	41.0	35.0	41.0
20	38.01991417737921	40.0	35.0	41.0	34.0	41.0
21	37.96868717350389	40.0	35.0	41.0	34.0	41.0
22	37.92099119516965	40.0	35.0	41.0	34.0	41.0
23	37.91939765030967	40.0	35.0	41.0	34.0	41.0
24	37.831618848709674	40.0	35.0	41.0	34.0	41.0
25	37.7431191752287	40.0	35.0	41.0	34.0	41.0
26	37.646542143321504	40.0	35.0	41.0	34.0	41.0
27	37.67012191388184	40.0	35.0	41.0	34.0	41.0
28	37.64679257846632	40.0	35.0	41.0	34.0	41.0
29	37.559190878147355	40.0	35.0	41.0	34.0	41.0
30	37.51294691031598	40.0	35.0	41.0	33.0	41.0
31	37.34929505990093	40.0	35.0	41.0	33.0	41.0
32	37.08792913602075	40.0	35.0	41.0	33.0	41.0
33	36.754448523844324	40.0	35.0	41.0	32.0	41.0
34	36.46686574366221	39.0	35.0	41.0	31.0	41.0
35	36.301430413679995	39.0	35.0	41.0	30.0	41.0
36	36.16793638140649	39.0	35.0	41.0	30.0	41.0
37	36.069719970974454	39.0	35.0	41.0	30.0	41.0
38	35.934015656046576	39.0	35.0	41.0	30.0	41.0
39	35.905519216589	39.0	35.0	41.0	30.0	41.0
40	35.82691521468672	39.0	35.0	41.0	30.0	41.0
41	35.769296431244186	38.0	35.0	41.0	29.0	41.0
42	35.70131520617999	38.0	35.0	41.0	29.0	41.0
43	35.611453722848594	38.0	35.0	41.0	29.0	41.0
44	35.4211736131558	38.0	35.0	40.0	28.0	41.0
45	35.324630681495265	38.0	35.0	40.0	27.0	41.0
46	35.27957032222533	38.0	35.0	40.0	27.0	41.0
47	35.20794110410732	37.0	35.0	40.0	27.0	41.0
48	35.106844139505945	37.0	35.0	40.0	27.0	41.0
49	35.064350832137684	37.0	35.0	40.0	27.0	41.0
50	34.97122892522256	37.0	35.0	40.0	27.0	41.0
51	34.85621245993679	37.0	35.0	40.0	26.0	41.0
52	34.49383830543041	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	5.0
13	7.0
14	18.0
15	46.0
16	95.0
17	221.0
18	521.0
19	1066.0
20	1987.0
21	3230.0
22	5149.0
23	8226.0
24	14902.0
25	26046.0
26	39344.0
27	46176.0
28	46037.0
29	43858.0
30	44056.0
31	48326.0
32	57821.0
33	76066.0
34	151408.0
35	233699.0
36	266598.0
37	262890.0
38	387935.0
39	959133.0
40	2383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.92512979177216	16.577449910221016	22.517382875736132	30.98003742227069
2	36.50559739048779	19.25219259085974	23.00138637669479	21.240823641957675
3	25.85117699018023	20.134472306016345	34.16518379482945	19.849166908973974
4	22.70519090088085	22.646083806672866	32.87795448387077	21.770770808575516
5	21.20606339052519	30.814632892511256	29.710481572483378	18.26882214448018
6	75.59313345699867	2.100758528820025	19.131668385734656	3.174439628446646
7	75.10542659591904	2.1033618809842727	17.56459705058533	5.2266144725113515
8	65.16150133485965	3.7797740070319845	23.606390752893113	7.452333905215247
9	37.776693251414514	16.161756903374936	27.88406502807037	18.177484817140176
10	24.704638696886576	16.993509586386008	37.19955574345321	21.102295973274206
11	21.605806281998774	13.94381086023189	41.040673527538516	23.40970933023082
12	19.686035728991776	14.809590456037633	43.979308116995384	21.525065697975215
13	16.90950564542417	18.4578035114454	43.58110523666121	21.05158560646922
14	15.71783035897293	18.69800858226208	43.38937384980418	22.19478720896081
15	18.095680892091785	15.662719960341045	43.91767100448693	22.323928143080234
16	18.307029087510397	19.657875525299634	39.13751309467805	22.897582292511917
17	18.318505837192223	19.876593774028297	39.51331247962694	22.291587909152543
18	19.901710622373503	24.495710518972754	35.88893292994819	19.71364592870555
19	21.06471236808613	19.679399014319536	35.01904663777068	24.23684197982365
20	19.864017016389752	24.189651638480186	36.26161562568636	19.684715719443705
21	19.41059373662803	19.078024664378407	35.888676261424955	25.622705337568608
22	19.69600913446607	21.788040933496085	33.37380140383016	25.142148528207684
23	18.514233919625354	19.81348998424422	38.44194139670943	23.230334699420993
24	22.795318219468456	18.287742281335834	35.12970743821713	23.787232060978575
25	17.91120955774913	20.064475133036797	37.02916451095663	24.99515079825744
26	17.464606327319103	18.96729053006817	36.9913975711091	26.576705571503634
27	21.38345800701292	19.551468088952507	37.43785413381156	21.627219770223004
28	21.13005284071555	20.31630362126286	34.152607037190904	24.40103650083069
29	20.146022389561953	19.1414584565495	37.3806903869938	23.331828766894745
30	23.25798156606666	19.174678696842573	31.97840464379359	25.588935093297177
31	22.49941607910964	18.40569980122856	36.34125620175319	22.753627917908606
32	20.774200266715262	20.020438147835936	34.33605169744061	24.86930988800819
33	20.447827905955187	18.722355425037573	37.97399801191895	22.855818657088285
34	19.814736659928506	20.366903987272174	36.84201649058595	22.976342862213368
35	22.86285870801132	21.728457169173524	36.018513867250306	19.390170255564847
36	25.136501820696505	21.031455460861167	33.20784687009236	20.62419584834997
37	22.52416625813593	22.5099394885623	36.172001644145226	18.79389260915654
38	22.169120356637244	19.285046161833904	34.53559314079038	24.010240340738463
39	24.481667084058575	18.26379877481114	37.66753579517558	19.586998345954704
40	21.092102566208563	17.853532473884894	38.43607468760691	22.61829027229964
41	22.029859349315963	21.13148285105929	33.30593091290027	23.532726886724483
42	20.62056582209278	17.69568133209497	35.340432295793605	26.343320550018646
43	23.412716018645867	19.529541263681807	35.30339869458389	21.754344023088436
44	21.53074907241829	20.349633862351606	35.428909602446126	22.69070746278398
45	22.263941042506875	19.71679928484816	34.44953585164266	23.569723821002306
46	23.032333267210632	18.993397385574422	34.62498712074017	23.34928222647477
47	21.042858876679208	19.92620413287656	36.05514413220922	22.975792858235007
48	22.04357278184312	18.519403957021957	32.773343727186294	26.66367953394863
49	21.13228952356089	17.138417301218478	37.60072864527053	24.1285645299501
50	21.000178567958308	16.898688900516383	37.722426192216126	24.378706339309186
51	19.896650585772573	15.4889920370424	37.5984552954933	27.015902081691724
52	17.740561656729316	16.702740816491907	40.6263005302405	24.930396996538278
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	881.0
1	4553.0
2	8225.0
3	88373.5
4	168522.0
5	96075.0
6	23628.0
7	22285.0
8	20942.0
9	20508.5
10	20075.0
11	19545.0
12	19015.0
13	18288.0
14	16652.5
15	15744.0
16	14974.5
17	14205.0
18	13525.0
19	12845.0
20	12261.0
21	11677.0
22	11316.5
23	10956.0
24	10873.0
25	10790.0
26	11824.5
27	12859.0
28	13497.5
29	14136.0
30	14891.0
31	15646.0
32	17996.5
33	20347.0
34	23907.0
35	27467.0
36	31027.5
37	34588.0
38	38158.5
39	47675.5
40	53622.0
41	66626.0
42	79630.0
43	111996.5
44	144363.0
45	189401.5
46	234440.0
47	256130.0
48	277820.0
49	267119.0
50	256418.0
51	224949.0
52	193480.0
53	166914.5
54	140349.0
55	130548.5
56	120748.0
57	116886.0
58	113024.0
59	111564.0
60	110104.0
61	107600.5
62	105097.0
63	105298.0
64	92278.0
65	79057.0
66	68318.0
67	57579.0
68	48307.0
69	39035.0
70	34536.0
71	30037.0
72	28814.5
73	27592.0
74	22658.0
75	17724.0
76	13528.5
77	9333.0
78	7303.0
79	5273.0
80	3885.0
81	2497.0
82	1980.5
83	1464.0
84	1064.0
85	664.0
86	500.5
87	337.0
88	237.0
89	104.0
90	71.0
91	42.5
92	14.0
93	10.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2727253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.404955619446515
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02056789148708	21.02550945883918
2	10.759692973086242	6.112572027587705
3	3.9980553347520638	3.4069375304317124
4	1.9945182619514807	2.266168108516296
5	1.1864032786523997	1.6849866238443625
6	0.8157425991633944	1.39026793956769
7	0.6063364216250862	1.2056071402700208
8	0.4771323164936097	1.0842337819683752
9	0.4162823466030712	1.064203342237736
>10	5.13001957339076	32.4001594525479
>50	0.41895307735614445	7.830179962876629
>100	0.1631573708014629	8.428328918466782
>500	0.006764404367706567	1.2932305889189375
>1k	0.00533347267453787	3.1995069420241116
>5k	3.902540981369173E-4	0.7644689178017628
>10k+	6.504234968948622E-4	6.843639264100841
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	134761	4.941272408537088	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	16320	0.5984043284579759	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	12939	0.4744334317351562	TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	10855	0.3980195456747137	TruSeq Adapter, Index 20 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	10336	0.3789894080233847	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	7842	0.2875420798877112	TruSeq Adapter, Index 20 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	6527	0.23932506445129956	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	6320	0.23173500954990242	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	4948	0.18142797899571475	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	4365	0.16005115770337405	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4311	0.15807114338127046	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4144	0.15194776575550564	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT	3882	0.1423410296001141	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	3137	0.11502416534146265	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	3130	0.11476749681822698	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3119	0.11436416056742811	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	2996	0.1098541279448588	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	2898	0.10626076861955967	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4083229535360306	0.0
2	0.0	0.0	0.0	1.308092795204552	0.0
3	0.0	0.0	0.0	2.1549155872227477	0.0
4	0.0	0.0	0.0	2.7201363423195426	0.0
5	0.0	0.0	0.0	5.534800035053587	0.0
6	0.0	0.0	0.0	6.5227905148513905	0.0
7	0.0	0.0	0.0	9.243880197400095	0.0
8	3.666693189080734E-5	0.0	0.0	12.051907175461903	0.0
9	3.666693189080734E-5	0.0	0.0	14.753233381721461	0.0
10	3.666693189080734E-5	0.0	0.0	16.243010824444962	0.0
11	3.666693189080734E-5	0.0	0.0	17.55587032079532	0.0
12	3.666693189080734E-5	0.0	0.0	18.51320724553241	0.0
13	3.666693189080734E-5	0.0	0.0	18.9807106271402	0.0
14	3.666693189080734E-5	0.0	0.0	19.289592861388364	0.0
15	3.666693189080734E-5	0.0	0.0	20.14459237921821	0.0
16	3.666693189080734E-5	0.0	0.0	21.04421555315917	0.0
17	3.666693189080734E-5	0.0	0.0	22.19610721850888	0.0
18	3.666693189080734E-5	0.0	0.0	22.809764990633433	0.0
19	3.666693189080734E-5	0.0	0.0	23.50680336587768	0.0
20	3.666693189080734E-5	0.0	0.0	24.288689021517257	0.0
21	3.666693189080734E-5	0.0	0.0	25.0551378988308	0.0
22	3.666693189080734E-5	0.0	0.0	25.77531310809815	0.0
23	3.666693189080734E-5	0.0	0.0	26.42666448620645	0.0
24	1.1000079567242204E-4	0.0	0.0	26.932008141525557	0.0
25	1.1000079567242204E-4	0.0	0.0	27.443401840606647	0.0
26	1.1000079567242204E-4	0.0	0.0	27.93998209920385	0.0
27	1.1000079567242204E-4	0.0	0.0	28.471175941505976	0.0
28	1.1000079567242204E-4	0.0	0.0	28.976079593642392	0.0
29	1.1000079567242204E-4	0.0	0.0	29.50360674275544	0.0
30	1.1000079567242204E-4	0.0	0.0	30.005173704089792	0.0
31	1.1000079567242204E-4	0.0	0.0	30.48881053572954	0.0
32	1.4666772756322937E-4	0.0	0.0	31.030711122143785	0.0
33	1.4666772756322937E-4	0.0	0.0	31.51145126616416	0.0
34	1.4666772756322937E-4	0.0	0.0	32.05918189474904	0.0
35	1.4666772756322937E-4	0.0	0.0	32.529985300227004	0.0
36	1.4666772756322937E-4	0.0	0.0	33.031295593038124	0.0
37	1.4666772756322937E-4	0.0	0.0	33.57400285195396	0.0
38	1.4666772756322937E-4	0.0	0.0	34.149013677865604	0.0
39	1.4666772756322937E-4	0.0	0.0	34.76872149375214	0.0
40	1.4666772756322937E-4	0.0	0.0	35.316708790860254	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	6.3129864E-4	46.0	20
TACGATA	30	1.8623505E-6	46.0	22
CGTTTTT	33165	0.0	45.23715	1
CGGTCTA	610	0.0	42.983604	30
TCACGAC	645	0.0	42.790695	24
CACGACG	625	0.0	42.688	25
AATGCGG	380	0.0	42.36842	1
ATGTTCG	60	1.8189894E-12	42.166668	17
GCGCGAC	2280	0.0	41.964912	8
TACGCGG	340	0.0	41.941177	1
CACTTAC	4385	0.0	41.751423	36
TCGGCGT	1670	0.0	41.730537	4
CATGCGG	365	0.0	41.589043	1
TAGTAGG	530	0.0	41.226418	1
CGACGGT	660	0.0	41.121212	27
ACCCGCA	4555	0.0	41.05159	31
ACTTACT	4425	0.0	41.01017	37
CCGCACT	4505	0.0	40.996666	33
CGCACTT	4485	0.0	40.974358	34
GCACTTA	4485	0.0	40.820515	35
>>END_MODULE