FastQCFastQC Report
Thu 26 May 2016
SRR1043307_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043307_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2680962
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1282284.782910015136358No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC158880.5926230957395144No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC125360.4675933489545917TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT107560.4011992710079441TruSeq Adapter, Index 20 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG90350.3370058956449215No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG75930.28321923249937897No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC66370.2475603906358986TruSeq Adapter, Index 22 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC63010.23502757592237414No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT49190.18347891540424668No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC41890.15624988343736318No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC41380.15434758120406034No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40420.1507667770001962No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT38750.1445376696872242No Hit
GGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC31870.11887523955953125No Hit
CGTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT30990.11559283570598913No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29670.11066922992567593No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC29300.10928912830543663TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC28440.10608132453947501No Hit
AGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC26880.10026251770819579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT317000.044.2902221
ATACCGG1000.042.751
ACGTAGG1950.042.6923071
ACGGGTA2200.041.931824
TAGTAGG4700.040.691491
CAACTTC13950.040.6451615
CACTTAC41500.040.3373536
TCGGCGT13900.040.3057564
GCGCGAC24800.040.2822578
CGGTCTA5700.040.26315730
CATGCGG3700.040.1351361
TCACGAC5950.040.08403424
CGCACTT42000.040.0178634
CACGACG5700.039.8684225
ACTTACT41750.039.82634737
CACGAGG2600.039.807691
TCGATGG2150.039.767441
AGACACG4700.039.73404323
CCCGCAC43100.039.6229732
CTTACTG41800.039.61722638
Produced by FastQC (version 0.11.5)