FastQCFastQC Report
Thu 26 May 2016
SRR1043306_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043306_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences806650
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT509506.316246203433955No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT60700.7524948862579806Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT48170.597161098369801Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT38250.4741833508956797Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC25470.3157503254199467Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT17180.2129796070166739Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG15860.19661563255439163No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC15020.18620219426021198Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC14950.18533440773569704Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT13210.16376371412632493No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC10870.13475485030682452Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC9460.117275150313023Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT8740.10834934606086903Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG8600.10661377301183908No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT8310.10301865741027708No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT8130.10078720634723858No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC8110.10053926734023429No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC253.4162436E-546.020
TCGTTGG405.6079443E-946.01
CGAACTC301.8607079E-646.030
ACCGGGT206.31033E-446.03
CATATGG501.6370905E-1146.01
GATGTCC453.092282E-1046.024
TCCGCAC206.31033E-446.018
TAGACGG301.8607079E-646.01
GCGAACT301.8607079E-646.029
GACACGA253.4162436E-546.024
CGGCCAT206.31033E-446.07
GCAACGC253.4162436E-546.012
GTTTAAG206.31033E-446.020
TAGCGTT253.4162436E-546.016
CGGTTCA206.31033E-446.040
TTCACGG800.046.01
TCGGGCA253.4162436E-546.04
CGTCTCA253.4162436E-546.012
CGATTTA301.8607079E-646.018
CGCGAGC253.4162436E-546.016
Produced by FastQC (version 0.11.5)