##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043306_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	806650
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26871257670613	33.0	31.0	34.0	31.0	34.0
2	32.59828178268146	34.0	31.0	34.0	31.0	34.0
3	32.677157379284694	34.0	31.0	34.0	31.0	34.0
4	36.19578503688093	37.0	35.0	37.0	35.0	37.0
5	36.08987045186884	37.0	35.0	37.0	35.0	37.0
6	36.31288786958408	37.0	37.0	37.0	35.0	37.0
7	36.240526870389886	37.0	37.0	37.0	35.0	37.0
8	36.225909626231946	37.0	37.0	37.0	35.0	37.0
9	37.7668778280543	39.0	38.0	39.0	35.0	39.0
10	37.61926610053927	39.0	37.0	39.0	35.0	39.0
11	37.28156573482923	39.0	37.0	39.0	35.0	39.0
12	37.261002913283335	39.0	37.0	39.0	34.0	39.0
13	37.26872745304655	39.0	37.0	39.0	34.0	39.0
14	38.66818942540135	40.0	38.0	41.0	35.0	41.0
15	38.76953201512428	40.0	38.0	41.0	35.0	41.0
16	38.7780462406248	41.0	38.0	41.0	35.0	41.0
17	38.77831649414244	41.0	38.0	41.0	35.0	41.0
18	38.49224198847084	40.0	38.0	41.0	35.0	41.0
19	38.21582842620715	40.0	37.0	41.0	35.0	41.0
20	37.92355668505547	40.0	35.0	41.0	34.0	41.0
21	37.866121614082935	40.0	35.0	41.0	34.0	41.0
22	37.81985991446104	40.0	35.0	41.0	34.0	41.0
23	37.82734395338747	40.0	35.0	41.0	34.0	41.0
24	37.732628773321764	40.0	35.0	41.0	34.0	41.0
25	37.65796937953264	39.0	35.0	41.0	34.0	41.0
26	37.60183226926176	39.0	35.0	41.0	34.0	41.0
27	37.62617740035951	39.0	35.0	41.0	34.0	41.0
28	37.656722246327405	40.0	35.0	41.0	34.0	41.0
29	37.56258104506291	40.0	35.0	41.0	34.0	41.0
30	37.511557676811506	40.0	35.0	41.0	34.0	41.0
31	37.38440959523957	40.0	35.0	41.0	33.0	41.0
32	37.14916010661377	40.0	35.0	41.0	33.0	41.0
33	36.949508460918615	40.0	35.0	41.0	33.0	41.0
34	36.72394966838158	40.0	35.0	41.0	32.0	41.0
35	36.55849996900763	40.0	35.0	41.0	31.0	41.0
36	36.478235913965165	40.0	35.0	41.0	31.0	41.0
37	36.44187937767309	39.0	35.0	41.0	31.0	41.0
38	36.38196739602058	39.0	35.0	41.0	31.0	41.0
39	36.35187751813054	39.0	35.0	41.0	31.0	41.0
40	36.31235728010909	39.0	35.0	41.0	31.0	41.0
41	36.30029752680841	39.0	35.0	41.0	31.0	41.0
42	36.212802330626666	39.0	35.0	41.0	31.0	41.0
43	36.09996156945392	39.0	35.0	41.0	30.0	41.0
44	35.95060807041468	39.0	35.0	41.0	30.0	41.0
45	35.87909502262443	39.0	35.0	41.0	30.0	41.0
46	35.85910122109961	39.0	35.0	41.0	30.0	41.0
47	35.77793590776669	38.0	35.0	41.0	30.0	41.0
48	35.75020392983326	38.0	35.0	41.0	30.0	41.0
49	35.691699002045496	38.0	35.0	41.0	29.0	41.0
50	35.54785594743693	38.0	35.0	40.0	28.0	41.0
51	35.46853158123102	38.0	35.0	40.0	28.0	41.0
52	35.185143494700306	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	8.0
16	22.0
17	49.0
18	112.0
19	254.0
20	493.0
21	854.0
22	1442.0
23	2423.0
24	4536.0
25	8067.0
26	10904.0
27	11773.0
28	10749.0
29	10477.0
30	11495.0
31	13677.0
32	17399.0
33	22946.0
34	42329.0
35	87376.0
36	79152.0
37	59478.0
38	94908.0
39	314367.0
40	1357.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.692431661811195	19.635653629207216	24.55984627781566	28.11206843116593
2	32.821917808219176	20.707493956486704	26.65716233806484	19.81342589722928
3	20.911051881237217	21.703464947622887	37.52643649662183	19.85904667451807
4	23.036632988284882	24.335089567966282	34.58439223950908	18.043885204239757
5	19.525072832083307	30.18434265170768	31.08522903365772	19.205355482551294
6	72.98902869894006	1.8545837723919916	21.32932498605343	3.827062542614517
7	74.06024917870204	1.4526746420380587	17.634661873179198	6.8524143060807035
8	62.997334655674706	3.00055786276576	25.365524081076057	8.636583400483481
9	33.29077047046427	20.99349160106614	28.61724415793715	17.09849377053245
10	26.167110890720885	19.401599206595176	42.03818260707866	12.393107295605281
11	23.40754974276328	16.85092667203868	39.672224632740345	20.069298952457697
12	17.01580611169652	23.229405566230707	42.0927291886196	17.66205913345317
13	12.669807227422053	25.824955061054983	41.16295791235356	20.342279799169404
14	10.24409595239571	23.35597842930639	46.28227855947437	20.11764705882353
15	11.662802950474184	18.80468604723238	48.34141201264489	21.191098989648545
16	11.70904357528048	25.151180809520856	38.28872497365648	24.85105064154218
17	14.064712080828116	22.048100167358832	42.01413252339925	21.87305522841381
18	16.000123969503502	28.05281100849191	38.456827620405384	17.490237401599206
19	16.64513729622513	23.36874728816711	35.98115663546768	24.004958780140086
20	12.870265914585014	30.160912415545777	35.562759561147956	21.406062108721255
21	14.557490857249118	23.652637451187008	36.12607698506167	25.663794706502202
22	11.388582408727453	26.71815533378789	32.82030620467365	29.072956052811012
23	11.352011405194322	22.512737866484844	45.02398809892767	21.111262629393167
24	16.191656852414308	22.13847393541189	39.46345998884275	22.20640922333106
25	12.471703960825636	26.903489741523583	37.8954937085477	22.72931258910308
26	14.258724353808963	23.37209446476167	37.94880059505362	24.42038058637575
27	19.317795822227733	22.64290584516209	39.10890720882663	18.93039112378355
28	14.689146469968387	21.02485588545218	35.40581416971425	28.880183474865184
29	16.12235789995661	22.07190231203124	36.68220417777227	25.123535610239884
30	23.262753362672782	21.599206595177588	30.297154899894625	24.840885142255008
31	18.583524452984566	20.782371536601996	32.65939378912787	27.974710221285566
32	19.184032727948924	22.133019277257794	32.42992623814542	26.253021756647865
33	17.250232442819065	20.258228475794954	32.6261699621893	29.86536911919668
34	16.185210438232193	22.957788384057523	31.907518750387403	28.94948242732288
35	21.98537159858675	19.986735263125272	29.85235232132895	28.17554081695903
36	16.143432715551974	28.12806049711771	29.396144548441082	26.332362238889235
37	18.209508460918613	25.1594867662555	33.078286741461596	23.552718031364282
38	17.49098121862022	27.5073451930825	29.06911299820244	25.932560590094837
39	21.995661067377426	24.477902436000743	32.49525816649104	21.031178330130786
40	16.377735077171014	21.61904171573793	38.23405442261203	23.76916878447902
41	19.893014318477654	24.144424471579992	30.956920597533006	25.005640612409348
42	19.96008181987231	18.951465939378913	35.92214715180066	25.16630508894812
43	21.346184838529723	21.365895989586562	33.208082811628344	24.079836360255378
44	15.254943283952146	25.599082625674086	32.993367631562634	26.152606458811135
45	19.642595921403334	21.73383747598091	33.08845224074878	25.535114361866977
46	22.81906650963863	21.213165561271925	32.20628525382756	23.761482675261885
47	17.47424533564743	18.85030682452117	36.64662493026715	27.028822909564248
48	18.314758569391927	17.447839831401478	32.81274406496002	31.424657534246574
49	19.618297898716914	18.06843116593318	37.443748837785904	24.869522097563998
50	17.880989276637944	17.72937457385483	37.246017479699994	27.14361866980723
51	16.020702907084857	16.68579929337383	37.12787454286246	30.16562325667886
52	15.76879687596851	16.536663980660755	43.968263807103455	23.72627533626728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	1256.5
2	2239.0
3	28868.0
4	55497.0
5	30319.5
6	5142.0
7	4919.0
8	4696.0
9	5089.0
10	5482.0
11	5619.0
12	5756.0
13	5763.0
14	5405.0
15	5040.0
16	4500.5
17	3961.0
18	3703.5
19	3446.0
20	3222.5
21	2999.0
22	2768.5
23	2538.0
24	2857.5
25	3177.0
26	3569.0
27	3961.0
28	4031.5
29	4102.0
30	4918.0
31	5734.0
32	6812.0
33	7890.0
34	9431.0
35	10972.0
36	12997.5
37	15023.0
38	16192.0
39	18767.5
40	20174.0
41	24935.0
42	29696.0
43	36493.0
44	43290.0
45	49417.0
46	55544.0
47	65854.0
48	76164.0
49	93118.0
50	110072.0
51	112433.0
52	114794.0
53	96918.0
54	79042.0
55	61104.5
56	43167.0
57	33353.5
58	23540.0
59	18590.5
60	13641.0
61	11044.5
62	8448.0
63	6848.0
64	4211.0
65	3174.0
66	2761.0
67	2348.0
68	2046.0
69	1744.0
70	1685.5
71	1627.0
72	1602.0
73	1577.0
74	1348.0
75	1119.0
76	807.0
77	495.0
78	415.0
79	335.0
80	241.5
81	148.0
82	136.0
83	124.0
84	91.0
85	58.0
86	35.5
87	13.0
88	8.5
89	3.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	806650.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.533848730078162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.23585475186188	22.361725106113262
2	11.735236765093505	7.166438883940337
3	4.673033827097984	4.280571239614442
4	2.402745860795246	2.9346031460137394
5	1.3910491099866344	2.1237041550220876
6	0.8973987583150507	1.6440622762171038
7	0.6211944229825566	1.32772195803223
8	0.4708235502542926	1.150084404961834
9	0.3456824891238928	0.9499515148391249
>10	3.515789468790563	24.17345549321946
>50	0.5148599191978724	10.489308478782078
>100	0.1804453669911473	9.506643165326349
>500	0.011405124775964164	2.325263950952306
>1k	0.003665932963702767	2.474759418915756
>5k	4.0732588485586305E-4	0.7549396759884732
>10k+	4.0732588485586305E-4	6.336767132061402
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50950	6.316246203433955	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	6070	0.7524948862579806	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	4817	0.597161098369801	Illumina Single End Adapter 2 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	3825	0.4741833508956797	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2547	0.3157503254199467	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1718	0.2129796070166739	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	1586	0.19661563255439163	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1502	0.18620219426021198	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1495	0.18533440773569704	Illumina Single End Adapter 2 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	1321	0.16376371412632493	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1087	0.13475485030682452	Illumina Single End Adapter 2 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	946	0.117275150313023	Illumina Single End Adapter 2 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	874	0.10834934606086903	Illumina Single End Adapter 2 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	860	0.10661377301183908	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	831	0.10301865741027708	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	813	0.10078720634723858	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	811	0.10053926734023429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6109217132585384	0.0
2	0.0	0.0	0.0	2.196863571561396	0.0
3	0.0	0.0	0.0	3.6789189859294615	0.0
4	0.0	0.0	0.0	4.637451187007996	0.0
5	0.0	0.0	0.0	9.663918676005702	0.0
6	0.0	0.0	0.0	11.003037252835803	0.0
7	0.0	0.0	0.0	15.125023244281907	0.0
8	0.0	0.0	0.0	19.83474865183165	0.0
9	0.0	0.0	0.0	23.34556499101221	0.0
10	0.0	0.0	0.0	25.486642285997643	0.0
11	0.0	0.0	0.0	27.42961631438666	0.0
12	0.0	0.0	0.0	28.837785904667452	0.0
13	0.0	0.0	0.0	29.42366577821856	0.0
14	0.0	0.0	0.0	29.858922705014567	0.0
15	0.0	0.0	0.0	31.209694415173868	0.0
16	0.0	0.0	0.0	32.853158123101714	0.0
17	0.0	0.0	0.0	34.65282340544226	0.0
18	0.0	0.0	0.0	35.56102398809893	0.0
19	0.0	0.0	0.0	36.555507345193085	0.0
20	0.0	0.0	0.0	37.614330874604846	0.0
21	0.0	0.0	0.0	38.64228599764458	0.0
22	0.001859542552532077	0.0	0.0	39.73978801214901	0.0
23	0.001859542552532077	0.0	0.0	40.55129238207401	0.0
24	0.002851298580549185	0.0	0.0	41.21601685985247	0.0
25	0.002851298580549185	0.0	0.0	41.83239323126511	0.0
26	0.002851298580549185	0.0	0.0	42.44257112750263	0.0
27	0.002851298580549185	0.0	0.0	43.039608256368936	0.0
28	0.003099237587553462	0.0	0.0	43.65338126820802	0.0
29	0.003099237587553462	0.0	0.0	44.45496807785285	0.0
30	0.003099237587553462	0.0	0.0	45.107915452798615	0.0
31	0.003099237587553462	0.0	0.0	45.65015806111697	0.0
32	0.0032232070910556	0.0	0.0	46.19202876092481	0.0
33	0.0032232070910556	0.0	0.0	46.760304964978616	0.0
34	0.0032232070910556	0.0	0.0	47.399119816525136	0.0
35	0.0032232070910556	0.0	0.0	47.93888303477345	0.0
36	0.0032232070910556	0.0	0.0	48.40922333106056	0.0
37	0.0032232070910556	0.0	0.0	48.92444058761544	0.0
38	0.0032232070910556	0.0	0.0	49.431103948428685	0.0
39	0.0032232070910556	0.0	0.0	49.955618917746236	0.0
40	0.0032232070910556	0.0	0.0	50.45087708423728	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.4162436E-5	46.0	20
TCGTTGG	40	5.6079443E-9	46.0	1
CGAACTC	30	1.8607079E-6	46.0	30
ACCGGGT	20	6.31033E-4	46.0	3
CATATGG	50	1.6370905E-11	46.0	1
GATGTCC	45	3.092282E-10	46.0	24
TCCGCAC	20	6.31033E-4	46.0	18
TAGACGG	30	1.8607079E-6	46.0	1
GCGAACT	30	1.8607079E-6	46.0	29
GACACGA	25	3.4162436E-5	46.0	24
CGGCCAT	20	6.31033E-4	46.0	7
GCAACGC	25	3.4162436E-5	46.0	12
GTTTAAG	20	6.31033E-4	46.0	20
TAGCGTT	25	3.4162436E-5	46.0	16
CGGTTCA	20	6.31033E-4	46.0	40
TTCACGG	80	0.0	46.0	1
TCGGGCA	25	3.4162436E-5	46.0	4
CGTCTCA	25	3.4162436E-5	46.0	12
CGATTTA	30	1.8607079E-6	46.0	18
CGCGAGC	25	3.4162436E-5	46.0	16
>>END_MODULE