SRR1043305_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043305_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	785310
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46086	5.868510524506246	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	5943	0.7567712113687589	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	4598	0.5855012670155735	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	3674	0.467840725318664	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	2420	0.30815856158714394	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1734	0.22080452305458992	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1543	0.1964829175739517	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1533	0.19520953508805441	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1511	0.19240809361908034	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	1297	0.16515770842087837	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1056	0.1344691905107537	No Hit
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	913	0.11625982096242247	Illumina Single End Adapter 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	912	0.11613248271383275	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	869	0.1106569380244744	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	829	0.10556340808088527	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	794	0.10110656938024473	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	791	0.10072455463447556	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	95	0.0	45.000004	24
TCCGCGG	30	2.1646192E-6	45.000004	1
GATGTCC	35	1.211265E-7	45.000004	24
TAGACGG	35	1.211265E-7	45.000004	1
TAGCGTT	35	1.211265E-7	45.000004	16
ATTACGG	70	0.0	45.000004	1
CGATTTA	30	2.1646192E-6	45.000004	18
CCTCGTA	30	2.1646192E-6	45.000004	18
TTATCGG	35	1.211265E-7	45.000004	1
GAAGTAC	35	1.211265E-7	45.000004	10
CGTACCA	30	2.1646192E-6	45.000004	21
TCGTACC	30	2.1646192E-6	45.000004	20
CGATGGT	35	1.211265E-7	45.000004	9
GAACCGA	30	2.1646192E-6	45.000004	8
ATTGCGG	190	0.0	45.000004	1
AATACGG	30	2.1646192E-6	45.000004	1
AGTCGTA	35	1.211265E-7	45.000004	4
CTAGCGG	125	0.0	45.000004	1
GCATAAC	20	7.0319214E-4	45.0	7
CCGGGTA	20	7.0319214E-4	45.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content