##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043305_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	785310
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21743005946696	33.0	31.0	34.0	31.0	34.0
2	32.502088347276874	34.0	31.0	34.0	31.0	34.0
3	32.57189517515376	34.0	31.0	34.0	31.0	34.0
4	36.14141167182387	37.0	35.0	37.0	35.0	37.0
5	36.09435891558748	37.0	35.0	37.0	35.0	37.0
6	36.31217863009512	37.0	37.0	37.0	35.0	37.0
7	36.250450140708764	37.0	37.0	37.0	35.0	37.0
8	36.165952299092076	37.0	37.0	37.0	35.0	37.0
9	37.71358062421209	39.0	38.0	39.0	35.0	39.0
10	37.56051368249481	39.0	37.0	39.0	35.0	39.0
11	37.271823865734554	39.0	37.0	39.0	35.0	39.0
12	37.24712915918555	39.0	37.0	39.0	34.0	39.0
13	37.26143943156206	39.0	37.0	39.0	34.0	39.0
14	38.66282996523666	40.0	38.0	41.0	35.0	41.0
15	38.773544205472994	40.0	38.0	41.0	35.0	41.0
16	38.81298850135615	40.0	38.0	41.0	35.0	41.0
17	38.78379875463193	40.0	38.0	41.0	35.0	41.0
18	38.54419401255555	40.0	38.0	41.0	35.0	41.0
19	38.28882988883371	40.0	37.0	41.0	35.0	41.0
20	38.01811004571443	40.0	35.0	41.0	34.0	41.0
21	37.96955851829214	40.0	35.0	41.0	34.0	41.0
22	37.937513848034534	40.0	35.0	41.0	34.0	41.0
23	37.912447313799646	40.0	35.0	41.0	34.0	41.0
24	37.83154550432313	40.0	35.0	41.0	34.0	41.0
25	37.81568297869631	40.0	35.0	41.0	34.0	41.0
26	37.78764691650431	40.0	35.0	41.0	34.0	41.0
27	37.77536641581032	40.0	35.0	41.0	34.0	41.0
28	37.73688225032153	40.0	35.0	41.0	34.0	41.0
29	37.70727865428939	40.0	35.0	41.0	34.0	41.0
30	37.60517247965772	40.0	35.0	41.0	34.0	41.0
31	37.47834613082732	40.0	35.0	41.0	34.0	41.0
32	37.24801543339573	40.0	35.0	41.0	33.0	41.0
33	37.01922043524213	40.0	35.0	41.0	33.0	41.0
34	36.742387082808065	40.0	35.0	41.0	32.0	41.0
35	36.57172963543059	40.0	35.0	41.0	31.0	41.0
36	36.470792425920976	39.0	35.0	41.0	31.0	41.0
37	36.39148234455183	39.0	35.0	41.0	31.0	41.0
38	36.336162789217	39.0	35.0	41.0	31.0	41.0
39	36.26037488380385	39.0	35.0	41.0	30.0	41.0
40	36.208813080184896	39.0	35.0	41.0	31.0	41.0
41	36.164792247647426	39.0	35.0	41.0	30.0	41.0
42	36.057722428085725	39.0	35.0	41.0	30.0	41.0
43	35.9343927875616	39.0	35.0	41.0	30.0	41.0
44	35.82249684837835	38.0	35.0	40.0	30.0	41.0
45	35.80342667226955	38.0	35.0	40.0	30.0	41.0
46	35.716175777718355	38.0	35.0	40.0	29.0	41.0
47	35.57707020157645	38.0	35.0	40.0	29.0	41.0
48	35.58232290433077	38.0	35.0	40.0	29.0	41.0
49	35.51795851319861	38.0	35.0	40.0	28.0	41.0
50	35.40598999121366	38.0	35.0	40.0	27.0	41.0
51	35.00487323477353	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	1.0
15	8.0
16	16.0
17	47.0
18	109.0
19	211.0
20	458.0
21	733.0
22	1162.0
23	1913.0
24	3559.0
25	6710.0
26	10242.0
27	11920.0
28	11204.0
29	10667.0
30	11304.0
31	13622.0
32	16718.0
33	22419.0
34	43314.0
35	84719.0
36	73514.0
37	58663.0
38	100559.0
39	301385.0
40	128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.918401650303704	19.715526352650546	24.699418064203947	27.666653932841808
2	32.43381594529549	20.81853026193478	26.817689829494086	19.92996396327565
3	21.14668092855051	21.881167946416067	37.008824540627266	19.963326584406158
4	23.274630400733468	24.444868905273076	34.10538513453286	18.175115559460593
5	19.827456673160917	30.2541671441851	30.60562071029275	19.312755472361236
6	73.61296812723637	1.7955966433637671	20.72735607594453	3.8640791534553234
7	74.63714966064356	1.4207128395156052	17.071347620684826	6.870789879156002
8	63.41381110644204	2.932727203270046	24.78613541149355	8.867326278794362
9	33.462199640906135	21.066839846684747	28.200073856184183	17.270886656224928
10	26.55677375813373	19.379862729368018	41.513669760986105	12.549693751512143
11	23.665431485655347	16.847614317912672	39.34930154970648	20.137652646725495
12	17.119481478651743	23.237320293896676	41.741095872967364	17.902102354484217
13	12.734970903210197	25.893214144732653	40.87226700283964	20.499547949217504
14	10.364569405712395	23.30264481542321	45.952426430326874	20.380359348537517
15	11.748863506131336	18.73272975003502	48.06191185646433	21.456494887369317
16	11.811131909691715	25.07213711782608	38.086997491436506	25.029733481045703
17	14.21718811679464	22.018438578395795	41.6489029809884	22.115470323821164
18	16.174504335867365	28.064076606690353	38.16289108759598	17.5985279698463
19	16.728680393729864	23.4279456520355	35.69074632947499	24.15262762475965
20	12.950427219824018	30.148731074352803	35.233220002292086	21.66762170353109
21	14.67356839974023	23.62697533458125	35.81833925456189	25.88111701111663
22	11.5174899084438	26.70512281774077	32.349772701226264	29.427614572589167
23	11.5025913333588	22.48373253874266	44.589143140925245	21.424532986973297
24	16.194751117393132	22.20868192178885	39.122257452471	22.474309508347023
25	12.541671441850987	26.895748175879593	37.5105372400708	23.052043142198624
26	14.366173867644624	23.22675121926373	37.60960639747361	24.797468515618036
27	19.52986718620672	22.556570016935986	38.79359743286091	19.119965363996382
28	14.888260686862512	20.966115292050272	35.07761266251544	29.06801135857177
29	16.24275763711146	21.981128471559003	36.28834473010658	25.487769161222957
30	23.609147979778687	21.441596312284318	29.74061198762272	25.20864372031427
31	18.724070749130917	20.703798499955433	32.1907272287377	28.381403522175958
32	19.391577848238274	21.9154219352867	32.10872139664591	26.58427881982911
33	17.572550967134	20.13192242553896	32.16436821127962	30.131158396047418
34	16.603252218868985	22.82678178044339	31.466809285505086	29.10315671518254
35	22.14323006201373	19.776139358979254	29.488864270160832	28.59176630884619
36	16.287071347620685	27.892042632845627	28.972507672129478	26.84837834740421
37	18.42291579121621	25.06029466070724	32.645579452700204	23.871210095376348
38	17.82098789013256	27.3865097859444	28.536119494212475	26.25638282971056
39	22.22090639365346	24.47148259922833	32.04861774331156	21.25899326380665
40	16.502655002483095	21.55798347149533	37.92744266595357	24.011918860068
41	20.12249939514332	23.996128917242874	30.60409265130967	25.277279036304133
42	20.330570093338938	18.74686429562848	35.461792158510654	25.460773452521934
43	21.668513071271217	21.114591689905897	32.88612140428621	24.33077383453668
44	15.413530962295145	25.434796449809628	32.745285301345966	26.40638728654926
45	19.85407036711617	21.48107116934714	32.946861748863505	25.717996714673184
46	23.148692872878225	21.01921534171219	31.877220460709783	23.954871324699802
47	17.69848849498924	18.649450535457333	36.41899377315964	27.23306719639378
48	18.470412957940177	17.200850619500578	32.52155199857381	31.807184423985436
49	19.994651793559232	17.762284957532696	37.055557677859696	25.187505571048373
50	18.186448663585082	17.437699761877475	37.0097159083674	27.36613566617005
51	16.210159045472487	16.549133463218347	36.681183227005896	30.559524264303267
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	213.0
1	1142.5
2	2072.0
3	26246.0
4	50420.0
5	27853.0
6	5286.0
7	4972.5
8	4659.0
9	4931.0
10	5203.0
11	5332.5
12	5462.0
13	5368.5
14	5275.0
15	4879.5
16	4484.0
17	3955.0
18	3426.0
19	3196.5
20	2967.0
21	2802.0
22	2637.0
23	2484.0
24	2331.0
25	2727.5
26	3433.5
27	3743.0
28	4184.5
29	4626.0
30	5292.5
31	5959.0
32	7108.0
33	8257.0
34	10407.0
35	12557.0
36	13803.0
37	15049.0
38	16483.0
39	17917.0
40	21582.0
41	25247.0
42	30201.0
43	35155.0
44	40199.0
45	45243.0
46	50249.0
47	55255.0
48	69881.0
49	84507.0
50	102423.0
51	120339.0
52	116517.0
53	112695.0
54	87342.0
55	61989.0
56	46738.0
57	31487.0
58	24427.0
59	17367.0
60	13610.5
61	9854.0
62	7947.0
63	6040.0
64	4964.5
65	3889.0
66	3222.5
67	2556.0
68	2321.5
69	2087.0
70	1842.5
71	1598.0
72	1612.5
73	1627.0
74	1388.0
75	958.5
76	768.0
77	596.0
78	424.0
79	298.0
80	172.0
81	144.0
82	116.0
83	86.0
84	56.0
85	33.5
86	11.0
87	7.0
88	3.0
89	4.5
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.274138870233042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.55583263486847	21.965653530333945
2	11.983435073090435	7.255763550903222
3	4.8109954505535315	4.36946233122351
4	2.457661975782641	2.9761439980373994
5	1.452467540035623	2.1986102005772112
6	0.9053831854571587	1.644581777238239
7	0.6421826295728691	1.3609068275418326
8	0.46666824904510434	1.130238350233599
9	0.3616924032588682	0.9854933440110575
>10	3.68391697320657	25.289593033917647
>50	0.480756855155571	9.817157840061611
>100	0.18245847097753734	9.4944667201266
>500	0.011881016714816386	2.3405382714207366
>1k	0.0038188982297624094	2.4877532472192088
>5k	4.243220255291566E-4	0.7634368247559516
>10k+	4.243220255291566E-4	5.920200152398247
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46086	5.868510524506246	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	5943	0.7567712113687589	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	4598	0.5855012670155735	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	3674	0.467840725318664	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	2420	0.30815856158714394	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1734	0.22080452305458992	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1543	0.1964829175739517	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCC	1533	0.19520953508805441	No Hit
ACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1511	0.19240809361908034	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	1297	0.16515770842087837	No Hit
AGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1056	0.1344691905107537	No Hit
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	913	0.11625982096242247	Illumina Single End Adapter 2 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	912	0.11613248271383275	No Hit
ACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	869	0.1106569380244744	Illumina Single End Adapter 2 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	829	0.10556340808088527	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	794	0.10110656938024473	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	791	0.10072455463447556	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6003998421005717	0.0
2	0.0	0.0	0.0	2.1974761559129514	0.0
3	0.0	0.0	0.0	3.7231157122664933	0.0
4	0.0	0.0	0.0	4.727559817142275	0.0
5	0.0	0.0	0.0	9.79383937553323	0.0
6	0.0	0.0	0.0	11.137639912900639	0.0
7	0.0	0.0	0.0	15.267601329411315	0.0
8	0.0	0.0	0.0	20.140199411697292	0.0
9	0.0	0.0	0.0	23.78169130661777	0.0
10	0.0	0.0	0.0	25.98782646343482	0.0
11	0.0	0.0	0.0	27.991875819739974	0.0
12	0.0	0.0	0.0	29.463014605697115	0.0
13	0.0	0.0	0.0	30.07627561090525	0.0
14	0.0	0.0	0.0	30.529345099387502	0.0
15	0.0	0.0	0.0	31.924335612687983	0.0
16	0.0	0.0	0.0	33.60621920006112	0.0
17	0.0	0.0	0.0	35.41722377150425	0.0
18	0.0	0.0	0.0	36.34602895671773	0.0
19	0.0	0.0	0.0	37.35875004775184	0.0
20	0.0	0.0	0.0	38.44367192573633	0.0
21	0.0	0.0	0.0	39.47829519552788	0.0
22	0.001018705988717831	0.0	0.0	40.59021278221339	0.0
23	0.001018705988717831	0.0	0.0	41.42911716392253	0.0
24	0.002164750226025391	0.0	0.0	42.12412932472527	0.0
25	0.00229208847461512	0.0	0.0	42.738663712419296	0.0
26	0.00229208847461512	0.0	0.0	43.36007436553717	0.0
27	0.00229208847461512	0.0	0.0	43.97753753294877	0.0
28	0.002801441468974036	0.0	0.0	44.60149495103845	0.0
29	0.002801441468974036	0.0	0.0	45.402707211165016	0.0
30	0.002801441468974036	0.0	0.0	46.057862500159175	0.0
31	0.002801441468974036	0.0	0.0	46.602870204123214	0.0
32	0.002801441468974036	0.0	0.0	47.14112898091199	0.0
33	0.002801441468974036	0.0	0.0	47.69428633278578	0.0
34	0.002801441468974036	0.0	0.0	48.345494136073654	0.0
35	0.002801441468974036	0.0	0.0	48.892921266760894	0.0
36	0.002801441468974036	0.0	0.0	49.36305408055418	0.0
37	0.002801441468974036	0.0	0.0	49.87418981039335	0.0
38	0.002801441468974036	0.0	0.0	50.40340757153226	0.0
39	0.002801441468974036	0.0	0.0	50.942557716061174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	95	0.0	45.000004	24
TCCGCGG	30	2.1646192E-6	45.000004	1
GATGTCC	35	1.211265E-7	45.000004	24
TAGACGG	35	1.211265E-7	45.000004	1
TAGCGTT	35	1.211265E-7	45.000004	16
ATTACGG	70	0.0	45.000004	1
CGATTTA	30	2.1646192E-6	45.000004	18
CCTCGTA	30	2.1646192E-6	45.000004	18
TTATCGG	35	1.211265E-7	45.000004	1
GAAGTAC	35	1.211265E-7	45.000004	10
CGTACCA	30	2.1646192E-6	45.000004	21
TCGTACC	30	2.1646192E-6	45.000004	20
CGATGGT	35	1.211265E-7	45.000004	9
GAACCGA	30	2.1646192E-6	45.000004	8
ATTGCGG	190	0.0	45.000004	1
AATACGG	30	2.1646192E-6	45.000004	1
AGTCGTA	35	1.211265E-7	45.000004	4
CTAGCGG	125	0.0	45.000004	1
GCATAAC	20	7.0319214E-4	45.0	7
CCGGGTA	20	7.0319214E-4	45.0	4
>>END_MODULE