FastQCFastQC Report
Thu 26 May 2016
SRR1043304_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043304_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1140186
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT633575.5567249554020135No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT87910.7710145537657891TruSeq Adapter, Index 23 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT86950.7625948748712928Illumina PCR Primer Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT63950.5608734013573224Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC35270.30933549438424957TruSeq Adapter, Index 23 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT29970.262851850487552Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG29150.25566004143183657No Hit
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC23730.208123937673327TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC22310.1956698293085514Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC20170.1769009617729037TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT17830.15637799446756934TruSeq Adapter, Index 23 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT17000.1490984804233695TruSeq Adapter, Index 23 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC16220.1422574913215914TruSeq Adapter, Index 23 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC12560.11015746553632477TruSeq Adapter, Index 23 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC12490.10954353061693443Illumina PCR Primer Index 7 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTG12350.10831566077815374Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT12150.10656156100846703No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG11660.10226401657273461TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC11570.10147467167637561No Hit
AGACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT11500.10086073675698527Illumina PCR Primer Index 7 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC750.046.020
TCGTTAC206.311433E-446.017
TCCGCGA253.4171382E-546.01
AGTCACG206.311433E-446.018
ATTACGC253.4171382E-546.013
CACGAAA206.311433E-446.034
TACGCTT301.86139E-646.011
TCTACGT253.4171382E-546.015
CGTAGGC206.311433E-446.02
ACTACGG900.046.01
GTCGTCA253.4171382E-546.017
CATACTC253.4171382E-546.024
GCGATCG405.6115823E-946.08
GCGATCC405.6115823E-946.08
ACGTCCG253.4171382E-546.039
TCATCCG253.4171382E-546.026
CGTAAGG206.311433E-446.01
CGTCGAA206.311433E-446.027
GCCAGAC600.046.036
CGGTATA253.4171382E-546.028
Produced by FastQC (version 0.11.5)