##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043304_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1140186
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.265320745913385	33.0	31.0	34.0	31.0	34.0
2	32.599044366445476	34.0	31.0	34.0	31.0	34.0
3	32.677952544584834	34.0	31.0	34.0	31.0	34.0
4	36.1913389569772	37.0	35.0	37.0	35.0	37.0
5	36.085063314231185	37.0	35.0	37.0	35.0	37.0
6	36.32855779671036	37.0	37.0	37.0	35.0	37.0
7	36.258816543967384	37.0	37.0	37.0	35.0	37.0
8	36.24024588970571	37.0	37.0	37.0	35.0	37.0
9	37.800803553104494	39.0	38.0	39.0	35.0	39.0
10	37.59279626306585	39.0	37.0	39.0	35.0	39.0
11	37.342770390094245	39.0	37.0	39.0	35.0	39.0
12	37.34571640065744	39.0	37.0	39.0	34.0	39.0
13	37.34923775594508	39.0	37.0	39.0	35.0	39.0
14	38.76038120096195	40.0	38.0	41.0	35.0	41.0
15	38.844764801532385	40.0	38.0	41.0	35.0	41.0
16	38.86024648609963	41.0	38.0	41.0	35.0	41.0
17	38.85657252413203	41.0	38.0	41.0	35.0	41.0
18	38.61304822195677	40.0	38.0	41.0	35.0	41.0
19	38.35404048111449	40.0	37.0	41.0	35.0	41.0
20	38.08046669578472	40.0	35.0	41.0	34.0	41.0
21	38.00886258908634	40.0	35.0	41.0	34.0	41.0
22	37.956812309570545	40.0	35.0	41.0	34.0	41.0
23	37.95471177509634	40.0	35.0	41.0	34.0	41.0
24	37.88133602763058	40.0	35.0	41.0	34.0	41.0
25	37.79368629328899	40.0	35.0	41.0	34.0	41.0
26	37.732708522995374	40.0	35.0	41.0	34.0	41.0
27	37.76269222740851	40.0	35.0	41.0	34.0	41.0
28	37.73797959280328	40.0	35.0	41.0	34.0	41.0
29	37.659273136137436	40.0	35.0	41.0	34.0	41.0
30	37.57157691815195	40.0	35.0	41.0	34.0	41.0
31	37.46850776978493	40.0	35.0	41.0	33.0	41.0
32	37.156659527480606	39.0	35.0	41.0	33.0	41.0
33	37.02426358506419	40.0	35.0	41.0	33.0	41.0
34	36.81108696300428	40.0	35.0	41.0	32.0	41.0
35	36.63937462835011	39.0	35.0	41.0	31.0	41.0
36	36.595773847424894	39.0	35.0	41.0	31.0	41.0
37	36.61532942870725	40.0	35.0	41.0	31.0	41.0
38	36.59677806954304	40.0	35.0	41.0	31.0	41.0
39	36.585247494707005	40.0	35.0	41.0	31.0	41.0
40	36.556003143346786	40.0	35.0	41.0	31.0	41.0
41	36.53432247019346	40.0	35.0	41.0	31.0	41.0
42	36.436538424432506	39.0	35.0	41.0	31.0	41.0
43	36.309291641890006	39.0	35.0	41.0	31.0	41.0
44	36.11696161854294	39.0	35.0	41.0	30.0	41.0
45	36.06967459695173	39.0	35.0	41.0	30.0	41.0
46	36.0572003164396	39.0	35.0	41.0	30.0	41.0
47	35.98506910276043	39.0	35.0	41.0	30.0	41.0
48	35.9626359208059	39.0	35.0	41.0	30.0	41.0
49	35.93501235763288	39.0	35.0	41.0	30.0	41.0
50	35.796597221856786	39.0	35.0	41.0	29.0	41.0
51	35.73436000792853	39.0	35.0	41.0	28.0	41.0
52	35.39169310972069	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	4.0
14	4.0
15	10.0
16	24.0
17	69.0
18	154.0
19	307.0
20	624.0
21	1058.0
22	1736.0
23	3224.0
24	5806.0
25	10420.0
26	14694.0
27	16144.0
28	14832.0
29	14524.0
30	15903.0
31	18988.0
32	23905.0
33	31696.0
34	57260.0
35	107788.0
36	108994.0
37	87829.0
38	145283.0
39	458233.0
40	668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.60768857011049	19.932274207892398	25.21790304388933	27.24213417810778
2	33.622233565400734	19.603731321030075	26.491818001624296	20.28221711194489
3	21.14926862810103	22.04008819613642	37.64043761281054	19.17020556295201
4	23.189725185189083	23.729110864367744	34.47542769337634	18.60573625706683
5	20.093914501669026	30.19998491474198	30.483535142511837	19.22256544107716
6	73.35259334880449	1.603247189493644	20.937724195876815	4.106435265825049
7	74.42925978743818	1.3742494645610452	17.012487436260397	7.184003311740366
8	62.31386808818912	3.2107919234230202	25.303766227615494	9.171573760772365
9	32.88717805691352	22.449670492358266	27.71933702045105	16.943814430277165
10	24.147902184380442	19.16739900332051	44.27786343631654	12.406835375982515
11	21.976063554542854	16.69508308293559	40.38805949204779	20.940793870473765
12	17.11931211223432	21.486055783880875	43.484659520464206	17.9099725834206
13	13.172412220462276	24.722457563941322	42.01954768783339	20.085582527763012
14	11.980326016983193	23.49678034987274	44.28900196985404	20.233891663290024
15	12.185292575071086	19.71327485164701	47.8086031577304	20.2928294155515
16	12.087413807922568	25.166683330614482	39.298675830083866	23.447227031379093
17	13.815640606006388	22.13823007825039	41.282211849645584	22.763917466097634
18	15.420641895269718	27.944124905936402	40.006191972187	16.629041226606887
19	16.114914584111713	23.714814951244797	36.335738204117575	23.834532260525915
20	13.989208778216888	29.812679685595157	35.382121864327395	20.815989671860553
21	14.6542757058936	23.606674700443612	37.00755841590758	24.731491177755206
22	12.41665833469276	26.33710640193793	32.95085187855315	28.295383384816162
23	11.655466739637218	22.919769230634298	43.656561297893504	21.768202731834982
24	17.1350990101615	21.73522565616487	38.67886467646507	22.45081065720856
25	12.606276519795893	27.126539003285426	37.337329172608676	22.92985530431
26	14.489214917566082	24.63519110039941	36.44247517510301	24.4331188069315
27	19.48725909632288	25.555654954542504	36.64638927332909	18.310696675805527
28	15.252862252299188	24.369006460349453	34.43359241386932	25.94453887348205
29	17.042482542322045	27.495601594827512	33.02399783894908	22.437918023901364
30	19.40972788650273	26.892542094009226	31.008800318544516	22.68892970094353
31	21.834507703129137	24.09177099175047	31.697547593112002	22.37617371200839
32	14.934230029135598	27.785992811699145	36.20532088624137	21.074456272923893
33	15.3955582685632	25.89235440533387	32.468386736900825	26.243700589202113
34	17.023801379774877	22.170330104035656	39.27218892356159	21.53367959262787
35	18.145548182489524	21.343622882582316	39.19395607383357	21.316872861094595
36	15.425027144693937	21.699968250794168	36.204268426379556	26.670736178132337
37	15.370562346845166	20.818796231492055	43.172254351483005	20.63838707017978
38	14.299245912509011	19.983669331144217	38.55449900279428	27.16258575355249
39	19.39157295388647	18.793249522446338	38.012043649018665	23.803133874648523
40	13.644703583450418	19.13749160224735	40.78720489464	26.430599919662228
41	18.018551359164206	23.60685011042058	32.51487038079752	25.859728149617695
42	19.152313745301207	18.77404212996827	37.6893769963848	24.384267128345726
43	22.88635363002177	20.878523328649887	34.16047907972909	22.074643961599246
44	15.249529462736783	24.202366982229215	36.07227241871063	24.47583113632337
45	17.881293052186223	21.344587637455643	36.50193915729539	24.272180153062745
46	21.858100345031424	18.73255767041518	35.86265749623307	23.546684488320327
47	15.54553379887141	17.895939785263106	40.09372155069436	26.46480486517112
48	17.65510188688512	15.791809406535426	34.30887592024459	32.24421278633486
49	19.424988554499002	16.343561489090376	39.70632861655905	24.52512133985157
50	17.541085401855486	16.071851434765907	39.805873778488774	26.581189384889836
51	15.867937336539828	15.27075406994999	38.262529096129924	30.598779497380253
52	14.756978247408755	15.359072993353717	46.24526173799713	23.638687021240397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	456.0
1	1775.5
2	3095.0
3	36640.5
4	70186.0
5	38695.5
6	7205.0
7	6872.5
8	6540.0
9	6985.5
10	7431.0
11	7695.0
12	7959.0
13	7823.5
14	7241.5
15	6795.0
16	6285.5
17	5776.0
18	5313.0
19	4850.0
20	4546.5
21	4243.0
22	4026.0
23	3809.0
24	4164.5
25	4520.0
26	4876.5
27	5233.0
28	5882.5
29	6532.0
30	7187.5
31	7843.0
32	9421.0
33	10999.0
34	13429.5
35	15860.0
36	18209.0
37	20558.0
38	23549.0
39	31251.0
40	35962.0
41	50674.0
42	65386.0
43	88714.5
44	112043.0
45	142701.0
46	173359.0
47	176039.0
48	178719.0
49	151871.5
50	125024.0
51	99607.0
52	74190.0
53	58782.5
54	43375.0
55	35231.0
56	27087.0
57	23410.5
58	19734.0
59	17476.5
60	15219.0
61	12874.0
62	10529.0
63	8726.5
64	6145.5
65	5367.0
66	4204.5
67	3042.0
68	2699.5
69	2357.0
70	2221.5
71	2086.0
72	2218.0
73	2350.0
74	2014.5
75	1679.0
76	1250.0
77	821.0
78	676.5
79	532.0
80	332.5
81	133.0
82	108.5
83	84.0
84	64.5
85	45.0
86	29.0
87	13.0
88	8.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1140186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.521101714705004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22995138122843	20.315566884775084
2	12.141760903944581	6.925927954740639
3	5.133782535444432	4.392634016237603
4	2.658734968044508	3.033202018241615
5	1.637884727991836	2.3357138462008544
6	1.078708603530703	1.8459574681095947
7	0.7509517602100027	1.4992580075050375
8	0.571350641306073	1.3036439804361957
9	0.4237701765263691	1.087775307753036
>10	3.5787542017620155	22.36865954279354
>50	0.5635135943568015	10.762094429943188
>100	0.21324602327154807	11.486640107121984
>500	0.010492568438775635	2.0808224941125752
>1k	0.005863494127551091	2.883630411239634
>5k	9.25814862244909E-4	2.1019467019966336
>10k+	3.0860495408163637E-4	5.576526828792793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63357	5.5567249554020135	No Hit
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	8791	0.7710145537657891	TruSeq Adapter, Index 23 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	8695	0.7625948748712928	Illumina PCR Primer Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	6395	0.5608734013573224	Illumina PCR Primer Index 7 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	3527	0.30933549438424957	TruSeq Adapter, Index 23 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	2997	0.262851850487552	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCG	2915	0.25566004143183657	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2373	0.208123937673327	TruSeq Adapter, Index 23 (96% over 25bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2231	0.1956698293085514	Illumina PCR Primer Index 7 (95% over 23bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2017	0.1769009617729037	TruSeq Adapter, Index 23 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	1783	0.15637799446756934	TruSeq Adapter, Index 23 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1700	0.1490984804233695	TruSeq Adapter, Index 23 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1622	0.1422574913215914	TruSeq Adapter, Index 23 (96% over 25bp)
TGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1256	0.11015746553632477	TruSeq Adapter, Index 23 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1249	0.10954353061693443	Illumina PCR Primer Index 7 (95% over 23bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTTG	1235	0.10831566077815374	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCT	1215	0.10656156100846703	No Hit
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1166	0.10226401657273461	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	1157	0.10147467167637561	No Hit
AGACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1150	0.10086073675698527	Illumina PCR Primer Index 7 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7902219462438584	0.0
2	0.0	0.0	0.0	2.5801930562206516	0.0
3	0.0	0.0	0.0	4.242202587998801	0.0
4	0.0	0.0	0.0	5.291066545282963	0.0
5	0.0	0.0	0.0	10.515126479363893	0.0
6	0.0	0.0	0.0	11.71764957647261	0.0
7	0.0	0.0	0.0	15.805842204692919	0.0
8	0.0	0.0	0.0	20.91983237822601	0.0
9	0.0	0.0	0.0	24.598968940155377	0.0
10	0.0	0.0	0.0	26.92262490505935	0.0
11	0.0	0.0	0.0	29.084114346255788	0.0
12	0.0	0.0	0.0	30.616232790088635	0.0
13	0.0	0.0	0.0	31.245428377475253	0.0
14	0.0	0.0	0.0	31.695618083365346	0.0
15	0.0	0.0	0.0	33.1890586272766	0.0
16	0.0	0.0	0.0	34.93000264869065	0.0
17	0.0	0.0	0.0	36.903715709542126	0.0
18	0.0	0.0	0.0	37.94170424825423	0.0
19	0.0	0.0	0.0	39.04827808796109	0.0
20	0.0	0.0	0.0	40.19107408791197	0.0
21	0.0	0.0	0.0	41.31264548064965	0.0
22	0.002192624712108375	0.0	0.0	42.47219313340104	0.0
23	0.0025434446660457153	0.0	0.0	43.35152334794498	0.0
24	0.0034204945508890653	0.0	0.0	44.08631574146674	0.0
25	0.0034204945508890653	0.0	0.0	44.78076384028571	0.0
26	0.0034204945508890653	0.0	0.0	45.41890533649773	0.0
27	0.0034204945508890653	0.0	0.0	46.0539771581128	0.0
28	0.0037713145048264055	0.0	0.0	46.70773014227503	0.0
29	0.0037713145048264055	0.0	0.0	47.51829964584726	0.0
30	0.0037713145048264055	0.0	0.0	48.20274937597901	0.0
31	0.0037713145048264055	0.0	0.0	48.77195475124234	0.0
32	0.0037713145048264055	0.0	0.0	49.35975358406436	0.0
33	0.0037713145048264055	0.0	0.0	49.96623357943353	0.0
34	0.0037713145048264055	0.0	0.0	50.643930025451986	0.0
35	0.0037713145048264055	0.0	0.0	51.24137640700728	0.0
36	0.0037713145048264055	0.0	0.0	51.7787448714508	0.0
37	0.0037713145048264055	0.0	0.0	52.32900596920152	0.0
38	0.0037713145048264055	0.0	0.0	52.8587440996469	0.0
39	0.0037713145048264055	0.0	0.0	53.39532321919406	0.0
40	0.0037713145048264055	0.0	0.0	53.94584743191023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	75	0.0	46.0	20
TCGTTAC	20	6.311433E-4	46.0	17
TCCGCGA	25	3.4171382E-5	46.0	1
AGTCACG	20	6.311433E-4	46.0	18
ATTACGC	25	3.4171382E-5	46.0	13
CACGAAA	20	6.311433E-4	46.0	34
TACGCTT	30	1.86139E-6	46.0	11
TCTACGT	25	3.4171382E-5	46.0	15
CGTAGGC	20	6.311433E-4	46.0	2
ACTACGG	90	0.0	46.0	1
GTCGTCA	25	3.4171382E-5	46.0	17
CATACTC	25	3.4171382E-5	46.0	24
GCGATCG	40	5.6115823E-9	46.0	8
GCGATCC	40	5.6115823E-9	46.0	8
ACGTCCG	25	3.4171382E-5	46.0	39
TCATCCG	25	3.4171382E-5	46.0	26
CGTAAGG	20	6.311433E-4	46.0	1
CGTCGAA	20	6.311433E-4	46.0	27
GCCAGAC	60	0.0	46.0	36
CGGTATA	25	3.4171382E-5	46.0	28
>>END_MODULE