SRR1043303_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043303_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1121412
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58915	5.253644512453942	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	8402	0.7492340014196388	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	8337	0.7434377374238906	TruSeq Adapter, Index 23 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	6553	0.5843525840636626	Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	3498	0.31192817626349634	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2985	0.2661822773432066	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	2867	0.2556598288586175	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	2381	0.2123216088288693	Illumina PCR Primer Index 7 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	2372	0.2115190491986888	TruSeq Adapter, Index 23 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1894	0.16889421550687883	TruSeq Adapter, Index 23 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1782	0.15890680677574343	TruSeq Adapter, Index 23 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1626	0.1449957731859477	TruSeq Adapter, Index 23 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1527	0.13616761725396198	TruSeq Adapter, Index 23 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	1494	0.13322489860996672	Illumina PCR Primer Index 7 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1345	0.11993807806586695	Illumina PCR Primer Index 7 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1265	0.11280421468648455	TruSeq Adapter, Index 23 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1190	0.10611621776831352	TruSeq Adapter, Index 23 (95% over 23bp)
AGACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1129	0.10067664694153441	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.033215E-4	45.0	27
ACAACGT	20	7.033215E-4	45.0	42
AACAACG	20	7.033215E-4	45.0	41
GGTACGT	20	7.033215E-4	45.0	7
ACCGGCA	25	3.8907456E-5	45.0	39
TCCGCGG	70	0.0	45.0	1
TTTACGG	40	6.8139343E-9	45.0	1
CGTTGGT	20	7.033215E-4	45.0	21
ACTTCGT	70	0.0	45.0	33
CGTCTTA	70	0.0	45.0	37
TTCGTGG	55	1.8189894E-12	45.0	1
TACGGGT	35	1.2119017E-7	45.0	3
ATCGTGT	20	7.033215E-4	45.0	37
AATGCGG	135	0.0	45.0	1
ACTACGG	35	1.2119017E-7	45.0	1
TCCACCG	20	7.033215E-4	45.0	30
TTGCGAG	20	7.033215E-4	45.0	1
TCATCCG	20	7.033215E-4	45.0	26
AACTCGC	20	7.033215E-4	45.0	32
CGACTGA	20	7.033215E-4	45.0	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content