##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043303_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1121412
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22536944494976	33.0	31.0	34.0	31.0	34.0
2	32.514212439317575	34.0	31.0	34.0	31.0	34.0
3	32.57935085410179	34.0	31.0	34.0	31.0	34.0
4	36.145885722642525	37.0	35.0	37.0	35.0	37.0
5	36.09188505205937	37.0	35.0	37.0	35.0	37.0
6	36.32801325471816	37.0	37.0	37.0	35.0	37.0
7	36.266438204691944	37.0	37.0	37.0	35.0	37.0
8	36.17395836677332	37.0	37.0	37.0	35.0	37.0
9	37.737860839727055	39.0	38.0	39.0	35.0	39.0
10	37.535690718487054	39.0	37.0	39.0	35.0	39.0
11	37.333067596922454	39.0	37.0	39.0	35.0	39.0
12	37.34790603275157	39.0	37.0	39.0	34.0	39.0
13	37.35806643766965	39.0	37.0	39.0	35.0	39.0
14	38.76594953505045	40.0	38.0	41.0	35.0	41.0
15	38.861129540258176	40.0	38.0	41.0	35.0	41.0
16	38.90229906582059	40.0	38.0	41.0	35.0	41.0
17	38.87407661055883	40.0	38.0	41.0	35.0	41.0
18	38.663176424008306	40.0	38.0	41.0	35.0	41.0
19	38.432973786619016	40.0	37.0	41.0	35.0	41.0
20	38.17710529225655	40.0	35.0	41.0	35.0	41.0
21	38.09999447125588	40.0	35.0	41.0	34.0	41.0
22	38.06798839320428	40.0	35.0	41.0	34.0	41.0
23	38.03326431320514	40.0	35.0	41.0	34.0	41.0
24	37.96713339967826	40.0	35.0	41.0	34.0	41.0
25	37.943366042096926	40.0	35.0	41.0	34.0	41.0
26	37.90666588194169	40.0	35.0	41.0	34.0	41.0
27	37.90788220564788	40.0	35.0	41.0	34.0	41.0
28	37.78805826939608	40.0	35.0	41.0	34.0	41.0
29	37.78743583981623	40.0	35.0	41.0	34.0	41.0
30	37.651885301744585	40.0	35.0	41.0	34.0	41.0
31	37.545028945650664	40.0	35.0	41.0	34.0	41.0
32	37.25296233676829	40.0	35.0	41.0	33.0	41.0
33	37.070599387201135	40.0	35.0	41.0	33.0	41.0
34	36.82852332594978	40.0	35.0	41.0	32.0	41.0
35	36.683426786943606	39.0	35.0	41.0	32.0	41.0
36	36.62555242854544	39.0	35.0	41.0	31.0	41.0
37	36.59954503786298	40.0	35.0	41.0	31.0	41.0
38	36.580556477012905	40.0	35.0	41.0	31.0	41.0
39	36.515328888936445	39.0	35.0	41.0	31.0	41.0
40	36.45421040616651	39.0	35.0	41.0	31.0	41.0
41	36.381004483633134	39.0	35.0	41.0	31.0	41.0
42	36.27217472258189	39.0	35.0	41.0	31.0	41.0
43	36.124702607070375	39.0	35.0	41.0	30.0	41.0
44	36.01860957435804	39.0	35.0	41.0	30.0	41.0
45	35.98810963321242	39.0	35.0	41.0	30.0	41.0
46	35.91978505669638	39.0	35.0	41.0	30.0	41.0
47	35.786362193377634	39.0	35.0	40.0	29.0	41.0
48	35.79160112429687	38.0	35.0	40.0	29.0	41.0
49	35.7492322179538	39.0	35.0	40.0	29.0	41.0
50	35.63948664719122	38.0	35.0	40.0	28.0	41.0
51	35.227583617796135	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	6.0
16	20.0
17	63.0
18	138.0
19	280.0
20	510.0
21	974.0
22	1510.0
23	2584.0
24	4647.0
25	8770.0
26	13925.0
27	15984.0
28	15584.0
29	15109.0
30	16114.0
31	18981.0
32	23450.0
33	31376.0
34	57287.0
35	105209.0
36	103917.0
37	88347.0
38	155483.0
39	441057.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.10233883710893	19.930409162734126	25.266806490388905	26.700445509768045
2	33.36463315891037	19.615894960995604	26.53877433093279	20.480697549161235
3	21.299040852068643	22.203793075158817	37.17581049605319	19.321355576719352
4	23.443034317449786	23.91904135143908	33.92535482052983	18.712569510581304
5	20.411499074381226	30.24624312919783	30.04551404836046	19.29674374806048
6	73.75193060177705	1.5716792757701898	20.505398551112346	4.170991571340417
7	74.75914293765359	1.3787974446501376	16.599251657731504	7.26280795996476
8	62.47739457041658	3.1363138614532398	25.016497059064825	9.369794509065358
9	33.055112661537414	22.473274764314986	27.371920400352412	17.09969217379518
10	24.3571497362254	19.114295192132776	44.03814120055787	12.490413871083955
11	22.10802095929061	16.60317528259016	40.15963802777213	21.1291657303471
12	17.237108217140534	21.412290933216337	43.26108513195864	18.089515717684492
13	13.260871116057258	24.708938374121196	41.83618509521924	20.194005414602305
14	12.07335038326681	23.419938434759036	44.01994984894044	20.486761333033712
15	12.228333565183894	19.671449922062543	47.64734103077192	20.452875481981643
16	12.088063976486787	25.052968935591913	39.173559762157	23.685407325764306
17	13.945008614140031	22.073867588361814	41.041561888048285	22.93956190944987
18	15.57340210377631	27.982311585750818	39.771288340057	16.672997970415867
19	16.195742510335183	23.695038041326473	36.04009944605551	24.069120002282837
20	13.975595053379134	29.889193267059746	35.05535877982401	21.079852899737116
21	14.66570716204214	23.550131441432764	36.72967651496506	25.054484881560036
22	12.44876994360681	26.318962165555565	32.655259619123036	28.57700827171459
23	11.676172539619694	22.983791862402043	43.294257596672765	22.045778001305496
24	17.205095005225555	21.73509825113339	38.45812243849718	22.60168430514387
25	12.58217318880126	27.039393193580953	37.21531426451652	23.16311935310127
26	14.588037224499114	24.523457926257255	36.23324879705228	24.655256052191344
27	19.56925732915289	25.50846611236548	36.400537893298804	18.521738665182824
28	15.311321797876248	24.389430468017107	34.04252852653619	26.256719207570455
29	17.171030807589005	27.456724201274824	32.654457059492856	22.717787931643322
30	19.57086244841325	26.846511362460895	30.621484342953348	22.961141846172502
31	21.955088763095098	24.059310940136186	31.233480647612115	22.752119649156597
32	14.985482588022958	27.912756417801845	35.861306995109736	21.240453999065465
33	15.570459385132315	25.784100758686368	32.189239993864874	26.45619986231644
34	17.298816135372192	22.08349830392398	38.993251365243104	21.624434195460722
35	18.204995131138244	21.25597015191562	38.98950608696893	21.549528629977207
36	15.52756703156378	21.641644640863483	35.958862576822796	26.87192575074995
37	15.560204456524454	20.59858464150553	43.12491751470468	20.716293387265342
38	14.384989638063441	19.754826950309074	38.39980310537073	27.460380306256756
39	19.40178988632189	18.556248729280586	37.98648489582776	24.05547648856977
40	13.686584413221903	18.94085313872154	40.681569307266194	26.690993140790358
41	18.16691813535079	23.572870630954547	32.30552196694882	25.954689266745852
42	19.40080898010722	18.59709009712755	37.490859737545165	24.51124118522006
43	23.08874882737121	20.767568030304652	33.976451116984656	22.167232025339484
44	15.34262162345329	23.856709220161726	36.08201089340938	24.718658262975605
45	18.04064875353572	20.996832564659552	36.44922651086309	24.513292170941632
46	22.085727636230036	18.455393735754566	35.84017292484832	23.618705703167077
47	15.73685674845641	17.578285233259496	40.05468106280296	26.630176955481126
48	17.787039910398676	15.485744757502149	34.09433820932895	32.63287712277022
49	19.61776760013269	15.925190741672107	39.6846118999975	24.7724297581977
50	17.73826211954215	15.702168337774165	39.81881770482214	26.740751837861552
51	16.04459377998452	15.115229728235475	37.99344041262266	30.84673607915735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	347.0
1	1708.0
2	3069.0
3	34329.0
4	65589.0
5	36423.5
6	7258.0
7	7007.5
8	6757.0
9	7021.0
10	7285.0
11	7396.5
12	7508.0
13	7391.5
14	7275.0
15	6790.5
16	6306.0
17	5650.0
18	4994.0
19	4697.5
20	4401.0
21	4161.5
22	3922.0
23	3666.5
24	3411.0
25	3831.5
26	5001.0
27	5750.0
28	6108.5
29	6467.0
30	7673.5
31	8880.0
32	10180.5
33	11481.0
34	14549.0
35	17617.0
36	19605.5
37	21594.0
38	25499.5
39	29405.0
40	37710.5
41	46016.0
42	60143.5
43	74271.0
44	101410.0
45	128549.0
46	158523.5
47	188498.0
48	181324.5
49	174151.0
50	137945.5
51	101740.0
52	80253.5
53	58767.0
54	46451.0
55	34135.0
56	28724.0
57	23313.0
58	20512.0
59	17711.0
60	14888.5
61	12066.0
62	9921.5
63	7777.0
64	6772.5
65	5768.0
66	4974.5
67	4181.0
68	3300.5
69	2420.0
70	2288.0
71	2156.0
72	2237.0
73	2318.0
74	2109.5
75	1462.0
76	1023.0
77	832.0
78	641.0
79	448.0
80	255.0
81	181.5
82	108.0
83	85.0
84	62.0
85	35.5
86	9.0
87	5.5
88	2.0
89	2.5
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1121412.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.94509699858201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.34888097740632	19.659063026553206
2	12.263533651929178	6.854112748970712
3	5.324701720990839	4.463979182448166
4	2.873962889011124	3.2125268681496335
5	1.7302818000556102	2.4176446368717555
6	1.120993098182569	1.8795756518071713
7	0.7757502974864798	1.5174892116956973
8	0.5561261538122774	1.2432799449385943
9	0.46035141966558185	1.1578108568390657
>10	3.758857448115814	23.180130868300637
>50	0.5471446914321488	10.28122509431472
>100	0.22104882155001981	11.648672383783074
>500	0.010955772496626274	2.138708568102881
>1k	0.006122343453997035	2.9432793674797173
>5k	9.666858085258476E-4	2.0973769512126776
>10k+	3.2222860284194924E-4	5.305124638532324
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58915	5.253644512453942	No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	8402	0.7492340014196388	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT	8337	0.7434377374238906	TruSeq Adapter, Index 23 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	6553	0.5843525840636626	Illumina PCR Primer Index 7 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	3498	0.31192817626349634	TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	2985	0.2661822773432066	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC	2867	0.2556598288586175	No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	2381	0.2123216088288693	Illumina PCR Primer Index 7 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	2372	0.2115190491986888	TruSeq Adapter, Index 23 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1894	0.16889421550687883	TruSeq Adapter, Index 23 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC	1782	0.15890680677574343	TruSeq Adapter, Index 23 (96% over 25bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1626	0.1449957731859477	TruSeq Adapter, Index 23 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1527	0.13616761725396198	TruSeq Adapter, Index 23 (95% over 24bp)
TGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCTT	1494	0.13322489860996672	Illumina PCR Primer Index 7 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1345	0.11993807806586695	Illumina PCR Primer Index 7 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG	1265	0.11280421468648455	TruSeq Adapter, Index 23 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT	1190	0.10611621776831352	TruSeq Adapter, Index 23 (95% over 23bp)
AGACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC	1129	0.10067664694153441	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7659985803611875	0.0
2	0.0	0.0	0.0	2.575235506664812	0.0
3	0.0	0.0	0.0	4.264088488441358	0.0
4	0.0	0.0	0.0	5.318205976037353	0.0
5	0.0	0.0	0.0	10.589150107186297	0.0
6	0.0	0.0	0.0	11.81055669102881	0.0
7	0.0	0.0	0.0	15.985026020766677	0.0
8	0.0	0.0	0.0	21.240008132604252	0.0
9	0.0	0.0	0.0	25.018637218078638	0.0
10	8.917329224228027E-5	0.0	0.0	27.405182038358785	0.0
11	8.917329224228027E-5	0.0	0.0	29.622565123255324	0.0
12	8.917329224228027E-5	0.0	0.0	31.20119991582041	0.0
13	8.917329224228027E-5	0.0	0.0	31.86081475853656	0.0
14	8.917329224228027E-5	0.0	0.0	32.33744600557155	0.0
15	8.917329224228027E-5	0.0	0.0	33.87657702967331	0.0
16	8.917329224228027E-5	0.0	0.0	35.69642557775376	0.0
17	8.917329224228027E-5	0.0	0.0	37.7463412198193	0.0
18	8.917329224228027E-5	0.0	0.0	38.810624462730914	0.0
19	8.917329224228027E-5	0.0	0.0	39.92957093378705	0.0
20	8.917329224228027E-5	0.0	0.0	41.08962629256687	0.0
21	8.917329224228027E-5	0.0	0.0	42.24415290722767	0.0
22	0.0015159459681187645	0.0	0.0	43.42097284494905	0.0
23	0.0015159459681187645	0.0	0.0	44.34757252463858	0.0
24	0.0027643720595106882	0.0	0.0	45.07870434773304	0.0
25	0.0027643720595106882	0.0	0.0	45.771045788702104	0.0
26	0.0027643720595106882	0.0	0.0	46.436011028952784	0.0
27	0.0027643720595106882	0.0	0.0	47.09116720705682	0.0
28	0.0031210652284798094	0.0	0.0	47.76487142994724	0.0
29	0.0031210652284798094	0.0	0.0	48.58553323845295	0.0
30	0.0031210652284798094	0.0	0.0	49.274842787485774	0.0
31	0.0031210652284798094	0.0	0.0	49.86427824920725	0.0
32	0.0031210652284798094	0.0	0.0	50.470656636454756	0.0
33	0.0031210652284798094	0.0	0.0	51.09139192375327	0.0
34	0.0031210652284798094	0.0	0.0	51.76072665532382	0.0
35	0.0031210652284798094	0.0	0.0	52.35729598042468	0.0
36	0.0031210652284798094	0.0	0.0	52.900985543225865	0.0
37	0.0031210652284798094	0.0	0.0	53.43112076560622	0.0
38	0.0031210652284798094	0.0	0.0	53.968657371242685	0.0
39	0.0031210652284798094	0.0	0.0	54.51243610733611	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.033215E-4	45.0	27
ACAACGT	20	7.033215E-4	45.0	42
AACAACG	20	7.033215E-4	45.0	41
GGTACGT	20	7.033215E-4	45.0	7
ACCGGCA	25	3.8907456E-5	45.0	39
TCCGCGG	70	0.0	45.0	1
TTTACGG	40	6.8139343E-9	45.0	1
CGTTGGT	20	7.033215E-4	45.0	21
ACTTCGT	70	0.0	45.0	33
CGTCTTA	70	0.0	45.0	37
TTCGTGG	55	1.8189894E-12	45.0	1
TACGGGT	35	1.2119017E-7	45.0	3
ATCGTGT	20	7.033215E-4	45.0	37
AATGCGG	135	0.0	45.0	1
ACTACGG	35	1.2119017E-7	45.0	1
TCCACCG	20	7.033215E-4	45.0	30
TTGCGAG	20	7.033215E-4	45.0	1
TCATCCG	20	7.033215E-4	45.0	26
AACTCGC	20	7.033215E-4	45.0	32
CGACTGA	20	7.033215E-4	45.0	38
>>END_MODULE