FastQCFastQC Report
Thu 26 May 2016
SRR1043302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043302_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2345429
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1435566.120671314288346No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC138220.5893164960440073No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA115850.49393948825566664No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT86530.3689303747843145No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT68090.29030936344694297Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC64720.27594098989992877No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT57650.24579725073749834No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC53290.22720790098527816No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC41080.17514919445440472No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39650.16905222882466278No Hit
GGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT39500.16841268697538914No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT39450.1681995063589646No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT37480.1598001900718376No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG33330.14210619890860052No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG27950.11916796458131967No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT25310.10791202803410377No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23810.10151660954136749No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23640.10079179544552404No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA23570.10049334258252969No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAA206.3128053E-446.013
ACGCAAT253.418251E-546.043
CGTTTTT339750.045.4110371
ATTACGG1500.044.466671
TACGGGA3300.043.212123
CGACGGT3200.042.4062527
GCATAGG3800.042.3684231
TATGCGG3700.042.2702671
ACTACGG1200.042.1666681
CACTTAC40150.041.9327536
TAGCATA7450.041.67785329
ACTTACT39600.041.52777537
GTTTTTT378150.041.420072
TATACGG1000.041.41
TTACGGG7450.041.3691252
CGTTCAG14350.041.35191738
GTCCCCC17250.041.29
TCGGCGT17050.041.1436964
TCACGAC3300.041.12121224
ACCCGCA41250.041.09333431
Produced by FastQC (version 0.11.5)