##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043302_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2345429
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33946838723321	33.0	31.0	34.0	31.0	34.0
2	32.67002966195097	34.0	31.0	34.0	31.0	34.0
3	32.76875786902951	34.0	31.0	34.0	31.0	34.0
4	36.21673348457787	37.0	37.0	37.0	35.0	37.0
5	36.158876265280256	37.0	37.0	37.0	35.0	37.0
6	36.311076140015324	37.0	37.0	37.0	35.0	37.0
7	36.25459180388748	37.0	37.0	37.0	35.0	37.0
8	36.24297985571083	37.0	37.0	37.0	35.0	37.0
9	37.872436982743885	39.0	38.0	39.0	35.0	39.0
10	37.6773946258872	39.0	37.0	39.0	35.0	39.0
11	37.278071516980475	39.0	37.0	39.0	35.0	39.0
12	37.26809764866044	39.0	37.0	39.0	35.0	39.0
13	37.2524024389568	39.0	37.0	39.0	35.0	39.0
14	38.52059004983737	40.0	37.0	41.0	34.0	41.0
15	38.573975166163635	40.0	37.0	41.0	35.0	41.0
16	38.42372163045652	40.0	37.0	41.0	34.0	41.0
17	38.39983303694122	40.0	37.0	41.0	34.0	41.0
18	38.182206751941756	39.0	37.0	41.0	34.0	41.0
19	37.923443856113316	39.0	37.0	41.0	34.0	41.0
20	37.557137308355955	39.0	35.0	41.0	34.0	41.0
21	37.50892438014538	39.0	35.0	41.0	34.0	41.0
22	37.474330708795705	39.0	35.0	41.0	34.0	41.0
23	37.48517904400432	39.0	35.0	41.0	34.0	41.0
24	37.40207100705244	39.0	35.0	41.0	34.0	41.0
25	37.29452010698256	39.0	35.0	41.0	34.0	41.0
26	37.205767473669	39.0	35.0	41.0	33.0	41.0
27	37.24061781448085	39.0	35.0	41.0	34.0	41.0
28	37.23414607732743	39.0	35.0	41.0	33.0	41.0
29	37.11845423587753	39.0	35.0	41.0	33.0	41.0
30	37.06294669333414	39.0	35.0	41.0	33.0	41.0
31	36.92657078939503	39.0	35.0	41.0	33.0	41.0
32	36.647344686196	39.0	35.0	41.0	32.0	41.0
33	36.14558189567879	38.0	35.0	41.0	31.0	41.0
34	35.94002973443238	38.0	35.0	41.0	30.0	41.0
35	35.745726687953464	38.0	35.0	41.0	29.0	41.0
36	35.67617310095509	38.0	35.0	41.0	29.0	41.0
37	35.58216386000173	38.0	35.0	41.0	29.0	41.0
38	35.46859529749142	38.0	35.0	41.0	28.0	41.0
39	35.44183644015658	38.0	35.0	41.0	28.0	41.0
40	35.370552679275306	37.0	35.0	41.0	28.0	41.0
41	35.32897009459677	37.0	35.0	41.0	28.0	41.0
42	35.236775873411645	37.0	35.0	40.0	27.0	41.0
43	35.1150143534509	37.0	35.0	40.0	27.0	41.0
44	34.89838490101384	37.0	35.0	40.0	25.0	41.0
45	34.802033657808444	36.0	35.0	40.0	24.0	41.0
46	34.79724221027369	36.0	35.0	40.0	24.0	41.0
47	34.72565914380695	36.0	35.0	40.0	24.0	41.0
48	34.63119028544458	36.0	35.0	40.0	24.0	41.0
49	34.56749532814679	35.0	35.0	40.0	24.0	41.0
50	34.455682947554585	35.0	35.0	40.0	24.0	41.0
51	34.30850646086494	35.0	34.0	40.0	24.0	41.0
52	33.92647912172997	35.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	4.0
13	7.0
14	31.0
15	40.0
16	105.0
17	233.0
18	474.0
19	1013.0
20	1785.0
21	2774.0
22	4552.0
23	7416.0
24	12412.0
25	22530.0
26	35070.0
27	42624.0
28	44915.0
29	45553.0
30	47160.0
31	51533.0
32	59072.0
33	75491.0
34	174432.0
35	244584.0
36	238592.0
37	206270.0
38	303535.0
39	721378.0
40	1840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.94141285027174	16.630987337497746	22.099112784910567	33.328487027319945
2	37.93817676851442	18.713676687718962	23.19533015068885	20.152816393077767
3	24.978713915450008	19.856154247261376	36.54666161286485	18.61847022442376
4	22.133179047415204	22.41811625932825	34.51871704494146	20.929987648315084
5	20.723714083862696	29.709575519020188	31.545486987668355	18.021223409448762
6	74.67115824013432	1.9307768429570882	20.265333122426643	3.132731794481948
7	74.27042131737946	1.7879458299526438	18.959516574579744	4.982116278088145
8	64.96248660692777	2.959671770068504	24.39519593217275	7.682645690830974
9	41.50349466984505	13.67519545464817	28.21833447100722	16.602975404499563
10	28.957687484890826	13.251733478182457	37.702782731858434	20.087796305068284
11	26.29339877694017	10.804846362861548	41.56263097284121	21.33912388735707
12	21.066252698333653	12.193206445388029	43.64357224200775	23.096968614270565
13	16.280603676342366	14.742505528839287	46.67726032209886	22.29963047271949
14	17.240001722499382	15.391256780742458	45.89335255938253	21.475388937375637
15	21.468652429896622	13.558756201957085	44.49684897730863	20.475742390837667
16	19.664376964725854	17.934970532043394	41.92397211768082	20.476680385549937
17	20.377764579528947	19.694392795518432	41.04494316391585	18.882899461036764
18	19.61717877624946	26.360337490497475	36.79314104157491	17.229342691678156
19	21.654545927418823	21.81690428488775	35.289663426179175	21.238886361514247
20	20.62846498444421	26.268328736448645	35.09106436391807	18.012141915189076
21	18.04561127196773	20.59806542854207	37.66773583851824	23.68858746097196
22	20.701372755261403	21.0017016076803	35.176038157624895	23.1208874794334
23	21.52544374611212	18.876504042544028	37.46764451194217	22.130407699401687
24	23.028324455781863	17.104973120056073	34.870891423274806	24.995811000887258
25	17.200350127844416	17.68853373945662	39.20046183448742	25.910654298211544
26	16.453876881372235	17.89629956822398	38.46345380738449	27.186369743019295
27	20.38236928084372	20.98260062444866	36.47857172397885	22.156458370728764
28	23.753948637967724	21.661111890404698	32.17624579554529	22.408693676082287
29	22.215168312492086	20.505886130000096	35.355706781147504	21.923238776360314
30	26.38489589750958	18.138089023372697	31.43783077637396	24.039184302743763
31	26.735663283774525	21.17237400918979	29.483731973980028	22.60823073305566
32	25.875777949364487	21.73171731056451	28.602230125064544	23.790274615006464
33	19.143192993691134	23.92688075401131	32.71201132074346	24.2179149315541
34	22.09489180870536	25.898886728184905	29.642849986079305	22.363371477030427
35	23.14928313754115	28.949885074329686	27.3493676423375	20.551464145791666
36	26.897680552257174	25.480754267129807	25.628019436955885	21.99354574365713
37	23.9116596579986	28.16823702614746	27.546772893146628	20.373330422707316
38	23.40492933275746	25.763389128385466	27.266994652151055	23.564686886706014
39	22.492729475076843	24.140274551052283	31.728992862286603	21.63800311158428
40	18.459096395584773	23.248326851931992	34.90193052102622	23.390646231457016
41	19.451878526273873	24.595628347735104	30.39418375060597	25.558309375385058
42	19.346354121143722	18.95034980807349	33.09279453780098	28.610501532981814
43	21.64610397500841	20.025291748332606	34.436258782508446	23.89234549415054
44	22.826143959164828	20.477874197001913	34.18513201636033	22.510849827472928
45	21.496579090648236	21.998875259067745	33.459337289681336	23.045208360602686
46	22.11156253290976	20.239666176209127	32.8435437610774	24.805227529803716
47	19.930511646270254	20.72175282219159	34.8711898761378	24.476545655400354
48	21.897614466266084	18.966466262675187	31.698124309028326	27.4377949620304
49	20.737229734944012	17.209900619460235	35.788761885352315	26.264107760243434
50	19.80175055394983	16.96333591850361	38.2800758411361	24.95483768641046
51	18.224171356284927	16.037279320755392	37.083365132775285	28.655184190184396
52	15.795106140497111	17.355460344354913	40.65784980061217	26.191583714535803
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	839.0
1	3956.0
2	7073.0
3	100271.0
4	193469.0
5	108848.0
6	24227.0
7	21504.5
8	18782.0
9	17901.0
10	17020.0
11	16508.5
12	15997.0
13	15299.0
14	13566.5
15	12532.0
16	11632.0
17	10732.0
18	9793.5
19	8855.0
20	8544.5
21	8234.0
22	7842.0
23	7450.0
24	7287.5
25	7125.0
26	7721.0
27	8317.0
28	8703.5
29	9090.0
30	9681.0
31	10272.0
32	11942.0
33	13612.0
34	15872.0
35	18132.0
36	20863.5
37	23595.0
38	24937.0
39	28554.5
40	30830.0
41	35881.5
42	40933.0
43	52735.0
44	64537.0
45	85334.0
46	106131.0
47	142517.5
48	178904.0
49	205953.0
50	233002.0
51	229418.0
52	225834.0
53	200427.0
54	175020.0
55	158691.0
56	142362.0
57	132103.5
58	121845.0
59	119223.0
60	116601.0
61	115748.5
62	114896.0
63	112707.0
64	93593.0
65	76668.0
66	63584.5
67	50501.0
68	41446.5
69	32392.0
70	27336.0
71	22280.0
72	21457.0
73	20634.0
74	16431.5
75	12229.0
76	9553.0
77	6877.0
78	5172.5
79	3468.0
80	2481.0
81	1494.0
82	1040.0
83	586.0
84	466.5
85	347.0
86	270.5
87	194.0
88	127.0
89	44.0
90	28.0
91	18.0
92	8.0
93	8.0
94	8.0
95	6.0
96	4.0
97	3.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2345429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.023081735936973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13264464008641	22.256904493304845
2	9.40420457848141	5.646864054424403
3	3.5953840220537394	3.238335250986038
4	1.9487853455874165	2.3403416686546876
5	1.389487637485128	2.0858350455644983
6	1.110127128127867	1.999766250303836
7	0.9177628426695101	1.92878481877708
8	0.7772392224280775	1.8668093362667413
9	0.688268533193504	1.859754818550782
>10	5.629794195234323	30.457032209847686
>50	0.23072727538170976	4.820034603815586
>100	0.16222286688578275	8.560857218571941
>500	0.007670132646788659	1.5326054732404337
>1k	0.004545263790689575	2.792242649249113
>5k	7.101974672952462E-4	1.4084669459413186
>10k+	4.2611848037714774E-4	7.205365162500998
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	143556	6.120671314288346	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13822	0.5893164960440073	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11585	0.49393948825566664	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	8653	0.3689303747843145	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	6809	0.29030936344694297	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	6472	0.27594098989992877	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	5765	0.24579725073749834	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	5329	0.22720790098527816	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4108	0.17514919445440472	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3965	0.16905222882466278	No Hit
GGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	3950	0.16841268697538914	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	3945	0.1681995063589646	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	3748	0.1598001900718376	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCG	3333	0.14210619890860052	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	2795	0.11916796458131967	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT	2531	0.10791202803410377	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2381	0.10151660954136749	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2364	0.10079179544552404	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2357	0.10049334258252969	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3002009440490418	0.0
2	4.2636123284908645E-5	0.0	0.0	0.9666888232387337	0.0
3	4.2636123284908645E-5	0.0	0.0	1.661060727056756	0.0
4	4.2636123284908645E-5	0.0	0.0	2.124089025930864	0.0
5	4.2636123284908645E-5	0.0	0.0	4.440552240123235	0.0
6	4.2636123284908645E-5	0.0	0.0	5.6676625043861915	0.0
7	4.2636123284908645E-5	0.0	0.0	8.54781790452834	0.0
8	4.2636123284908645E-5	0.0	0.0	12.202032122908005	0.0
9	4.2636123284908645E-5	0.0	0.0	15.667538859628666	0.0
10	4.2636123284908645E-5	0.0	0.0	17.502341789071423	0.0
11	4.2636123284908645E-5	0.0	0.0	18.81719719505472	0.0
12	4.2636123284908645E-5	0.0	0.0	19.676954621094904	0.0
13	4.2636123284908645E-5	0.0	0.0	20.194045524294275	0.0
14	4.2636123284908645E-5	0.0	0.0	20.50989392558888	0.0
15	4.2636123284908645E-5	0.0	0.0	21.111958622495074	0.0
16	4.2636123284908645E-5	0.0	0.0	21.744849236536258	0.0
17	4.2636123284908645E-5	0.0	0.0	22.601707406193068	0.0
18	4.2636123284908645E-5	0.0	0.0	23.09091428476411	0.0
19	4.2636123284908645E-5	0.0	0.0	23.658742174672522	0.0
20	4.2636123284908645E-5	0.0	0.0	24.26822555703029	0.0
21	4.2636123284908645E-5	0.0	0.0	24.956244678478864	0.0
22	4.2636123284908645E-5	0.0	0.0	25.533793604496235	0.0
23	4.2636123284908645E-5	0.0	0.0	26.0692606768314	0.0
24	4.2636123284908645E-5	0.0	0.0	26.49310637840668	0.0
25	4.2636123284908645E-5	0.0	0.0	26.92138623680359	0.0
26	8.527224656981729E-5	0.0	0.0	27.320929348106464	0.0
27	8.527224656981729E-5	0.0	0.0	27.770484632022544	0.0
28	8.527224656981729E-5	0.0	0.0	28.17514407811961	0.0
29	8.527224656981729E-5	0.0	0.0	28.63036996643258	0.0
30	8.527224656981729E-5	0.0	0.0	29.02586264602339	0.0
31	8.527224656981729E-5	0.0	0.0	29.44885562513297	0.0
32	8.527224656981729E-5	0.0	0.0	29.93874468167657	0.0
33	8.527224656981729E-5	0.0	0.0	30.425265484480665	0.0
34	1.2790836985472593E-4	0.0	0.0	30.936771055529714	0.0
35	1.2790836985472593E-4	0.0	0.0	31.42341976670366	0.0
36	1.2790836985472593E-4	0.0	0.0	31.91684762147991	0.0
37	1.2790836985472593E-4	0.0	0.0	32.65018894198034	0.0
38	1.2790836985472593E-4	0.0	0.0	33.36135947837261	0.0
39	1.2790836985472593E-4	0.0	0.0	34.150937845485835	0.0
40	1.2790836985472593E-4	0.0	0.0	34.813844290319594	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAA	20	6.3128053E-4	46.0	13
ACGCAAT	25	3.418251E-5	46.0	43
CGTTTTT	33975	0.0	45.411037	1
ATTACGG	150	0.0	44.46667	1
TACGGGA	330	0.0	43.21212	3
CGACGGT	320	0.0	42.40625	27
GCATAGG	380	0.0	42.368423	1
TATGCGG	370	0.0	42.270267	1
ACTACGG	120	0.0	42.166668	1
CACTTAC	4015	0.0	41.93275	36
TAGCATA	745	0.0	41.677853	29
ACTTACT	3960	0.0	41.527775	37
GTTTTTT	37815	0.0	41.42007	2
TATACGG	100	0.0	41.4	1
TTACGGG	745	0.0	41.369125	2
CGTTCAG	1435	0.0	41.351917	38
GTCCCCC	1725	0.0	41.2	9
TCGGCGT	1705	0.0	41.143696	4
TCACGAC	330	0.0	41.121212	24
ACCCGCA	4125	0.0	41.093334	31
>>END_MODULE