FastQCFastQC Report
Thu 26 May 2016
SRR1043301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043301_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2290013
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1361145.943809052612365No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC133590.5833591337691096No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG95910.41881858312594733No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC79640.3477709515186158No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG65770.28720360976116727No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT65660.28672326314304764No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC56320.2459374684772532No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT51400.22445287428499314No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC41480.18113434290547695No Hit
GGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC40870.1784706025686317No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC39480.1724007680305745No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC39430.17218242865870192No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT36790.1606541098238307No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC31410.1371607934103431No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT27720.12104734776614806No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTC23650.10327452289572155No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC22950.10021777168950569No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC22940.10017410381513117No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA2300.045.00000430
CGATAGA207.0347567E-445.09
CGTTTTT320900.044.4320641
ATAACGG1250.043.21
CTATCGG750.042.01
TATACGG1350.041.6666681
TACGGGT650.041.538463
ACGGGTA1300.041.538464
ACTACGG650.041.538461
CACGACG2350.041.1702125
CTCGAGG2900.041.120691
CATATTA3350.040.9701528
CACTTAC38400.040.95702736
CGCACTT38350.040.71708334
GTTTTTT354750.040.6871032
GCACTTA38850.040.36679535
TCACGAC2400.040.31249624
ACTTACT38300.040.3002637
CCGCACT39000.039.98076633
ACCCGCA39500.039.9303831