##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043301_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2290013
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32901341608105	33.0	31.0	34.0	31.0	34.0
2	32.62565452685203	34.0	31.0	34.0	31.0	34.0
3	32.69904013645338	34.0	31.0	34.0	31.0	34.0
4	36.20076174240059	37.0	37.0	37.0	35.0	37.0
5	36.18374175168438	37.0	37.0	37.0	35.0	37.0
6	36.33865353602796	37.0	37.0	37.0	35.0	37.0
7	36.2920044558699	37.0	37.0	37.0	35.0	37.0
8	36.206837253762316	37.0	37.0	37.0	35.0	37.0
9	37.8403035266612	39.0	38.0	39.0	35.0	39.0
10	37.63332871909461	39.0	37.0	39.0	35.0	39.0
11	37.28219097446172	39.0	37.0	39.0	35.0	39.0
12	37.277749078280344	39.0	37.0	39.0	35.0	39.0
13	37.27565083691665	39.0	37.0	39.0	35.0	39.0
14	38.54537332320821	40.0	37.0	41.0	35.0	41.0
15	38.60532232786451	40.0	37.0	41.0	35.0	41.0
16	38.49459326213432	40.0	37.0	41.0	34.0	41.0
17	38.443227178186326	40.0	37.0	41.0	34.0	41.0
18	38.246341396315216	40.0	37.0	41.0	34.0	41.0
19	38.00909252480226	40.0	37.0	41.0	34.0	41.0
20	37.663803655263095	39.0	35.0	41.0	34.0	41.0
21	37.6166401675449	39.0	35.0	41.0	34.0	41.0
22	37.599512317178984	39.0	35.0	41.0	34.0	41.0
23	37.57384739737285	39.0	35.0	41.0	34.0	41.0
24	37.494114662231176	39.0	35.0	41.0	34.0	41.0
25	37.45323760170794	39.0	35.0	41.0	34.0	41.0
26	37.438403188104175	39.0	35.0	41.0	34.0	41.0
27	37.41300857244042	39.0	35.0	41.0	34.0	41.0
28	37.339242615653276	39.0	35.0	41.0	34.0	41.0
29	37.26192995410943	39.0	35.0	41.0	34.0	41.0
30	37.158225739329865	39.0	35.0	41.0	33.0	41.0
31	37.008220040672256	39.0	35.0	41.0	33.0	41.0
32	36.738238603885655	39.0	35.0	41.0	33.0	41.0
33	36.22881092814757	38.0	35.0	41.0	31.0	41.0
34	35.970218946355324	38.0	35.0	41.0	30.0	41.0
35	35.77531088251464	38.0	35.0	41.0	30.0	41.0
36	35.68320179841774	38.0	35.0	41.0	29.0	41.0
37	35.53911222338039	38.0	35.0	41.0	29.0	41.0
38	35.42878883220314	38.0	35.0	41.0	28.0	41.0
39	35.355824617589505	38.0	35.0	41.0	27.0	41.0
40	35.26241423083624	37.0	35.0	40.0	27.0	41.0
41	35.188746526766444	37.0	35.0	40.0	27.0	41.0
42	35.08066591761706	37.0	35.0	40.0	26.0	41.0
43	34.955469248427846	37.0	35.0	40.0	26.0	41.0
44	34.83155554138776	36.0	35.0	40.0	25.0	41.0
45	34.76389042332948	36.0	35.0	40.0	24.0	41.0
46	34.68966289711019	36.0	35.0	40.0	24.0	41.0
47	34.55635710364963	36.0	35.0	40.0	23.0	41.0
48	34.48901163443177	36.0	35.0	40.0	23.0	41.0
49	34.407996373819714	35.0	34.0	40.0	24.0	41.0
50	34.324837893933356	35.0	34.0	40.0	24.0	41.0
51	33.89767175994197	35.0	34.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	13.0
15	35.0
16	72.0
17	173.0
18	452.0
19	859.0
20	1510.0
21	2587.0
22	4046.0
23	6316.0
24	10606.0
25	19129.0
26	31849.0
27	41186.0
28	45036.0
29	44689.0
30	46021.0
31	50548.0
32	56962.0
33	72896.0
34	166945.0
35	243921.0
36	226200.0
37	202027.0
38	314462.0
39	701325.0
40	137.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.244468481183294	16.62759119708054	22.242930498647826	32.88500982308834
2	37.668519785695544	18.77727331678903	23.25052303196532	20.30368386555011
3	25.04383162890342	19.999406116908506	36.212327178928675	18.744435075259396
4	22.303235833159025	22.484894190556997	34.083605638919956	21.128264337364026
5	20.869488513820663	29.83860790309924	31.266241720025167	18.025661863054925
6	75.05219402684614	1.892871350511984	19.922157647139993	3.1327769755018857
7	74.62507854758903	1.749160375945464	18.572951332590687	5.052809743874817
8	65.28565558361458	2.904961674890055	24.110518149896965	7.698864591598388
9	41.75487213391365	13.596735040368765	27.861239215672573	16.787153610045007
10	29.310968977032008	13.220754642004215	37.37472232690382	20.093554054059958
11	26.60194505446039	10.708236154117902	41.31867373678665	21.37114505463506
12	21.21494506799743	12.178227809187108	43.5162158468096	23.090611276005856
13	16.34606441098806	14.67572454828859	46.525238066334126	22.45297297438923
14	17.362914533672953	15.338995892163057	45.79991467297347	21.498174901190517
15	21.58241023085895	13.478264097190715	44.36476998165513	20.574555690295206
16	19.734167447957716	17.855007809999332	41.86570119907616	20.545123542966788
17	20.491717732606755	19.596831983049878	40.93382875992407	18.977621524419295
18	19.735521152063328	26.543735777919164	36.603154654580564	17.117588415436945
19	21.79354440345972	21.97677480433517	34.925478588986174	21.30420220321893
20	20.72363781340979	26.476181576261794	34.674912325825225	18.12526828450319
21	18.055661692750217	20.680013606909657	37.445682622762405	23.818642077577724
22	20.837654633401645	21.09066629752757	34.78565405523899	23.2860250138318
23	21.687431468729653	18.942730892794057	37.11035701544053	22.259480623035767
24	23.117860029615553	17.11086356278327	34.58661588383996	25.18466052376122
25	17.061999211358188	17.65352423763533	39.150869449212735	26.133607101793743
26	16.51274468747557	17.769462444099663	38.28043770930558	27.437355159119186
27	20.39503705874159	21.04686741952993	36.296387837099616	22.261707684628863
28	23.83968999302624	21.718348323786806	31.865757967312845	22.5762037158741
29	22.34943644424726	20.611979058634162	35.005041456096535	22.03354304102204
30	26.572949585875715	18.109547849728365	31.05178878897194	24.265713775423983
31	26.99783800353972	21.23302356798848	29.072236707826548	22.696901720645254
32	26.095441379590422	21.712496828620623	28.134993120126396	24.057068671662563
33	19.267881885386675	23.956370553354937	32.348724657894955	24.42702290336343
34	22.174022592884842	25.94251648353088	29.29136210143785	22.592098822146426
35	23.222226249370635	29.12197441673912	26.946440915400917	20.709358418489327
36	27.097750100108602	25.455357677008823	25.297891322014326	22.14900090086825
37	24.093182003770284	28.055255581518534	27.274692327074124	20.576870087637054
38	23.629429177912964	25.779023961872703	27.02844918347625	23.563097676738078
39	22.625985092661047	24.296936305601758	31.451087832252483	21.625990769484716
40	18.564217757715788	23.29598128918919	34.6523360347736	23.487464918321425
41	19.567749178716454	24.583921575991052	30.207383102191997	25.640946143100496
42	19.47696366789184	18.88273123340348	32.92422357427665	28.716081524428027
43	21.81900277422006	19.909974310189504	34.20674904465608	24.064273870934358
44	22.89476085943617	20.352635552723935	34.17814658694077	22.57445700089912
45	21.661623754974315	21.905596169104715	33.36295470811738	23.06982536780359
46	22.212057311465045	20.198575291930656	32.723307684279526	24.866059712324777
47	20.163815663928546	20.56036363112349	34.69888598885683	24.57693471609113
48	22.13424115932966	18.86203266094996	31.444319311724435	27.559406867995946
49	20.915296114039528	17.090339661827247	35.550715214280444	26.44364900985278
50	19.960192365720193	16.82121455205713	38.17497979269113	25.043613289531546
51	18.252996817048636	16.035891499305897	36.838611833207935	28.87249985043753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	628.0
1	3576.0
2	6524.0
3	94780.5
4	183037.0
5	103858.0
6	24679.0
7	21458.5
8	18238.0
9	17518.0
10	16798.0
11	16101.0
12	15404.0
13	14609.0
14	13814.0
15	12762.5
16	11711.0
17	10819.5
18	9928.0
19	9118.5
20	8309.0
21	7899.5
22	7490.0
23	7306.5
24	7123.0
25	6959.5
26	7293.5
27	7791.0
28	8407.0
29	9023.0
30	9819.5
31	10616.0
32	12455.0
33	14294.0
34	16757.5
35	19221.0
36	21496.0
37	23771.0
38	25628.0
39	27485.0
40	31037.0
41	34589.0
42	41521.5
43	48454.0
44	59139.5
45	69825.0
46	98286.5
47	126748.0
48	170117.5
49	213487.0
50	234833.5
51	256180.0
52	228249.5
53	200319.0
54	177362.0
55	154405.0
56	141597.5
57	128790.0
58	123020.5
59	117251.0
60	116236.5
61	115222.0
62	111704.5
63	108187.0
64	103138.5
65	98090.0
66	79608.5
67	61127.0
68	49954.0
69	38781.0
70	31851.0
71	24921.0
72	22823.5
73	20726.0
74	17437.0
75	11172.0
76	8196.0
77	6290.0
78	4384.0
79	3127.0
80	1870.0
81	1343.5
82	817.0
83	658.5
84	500.0
85	350.5
86	201.0
87	126.5
88	52.0
89	41.0
90	30.0
91	23.0
92	16.0
93	13.0
94	10.0
95	7.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2290013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.930349843039593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.61835367936345	22.034230804919698
2	9.76968924184473	5.84820433732386
3	3.6026918060428264	3.234894783945418
4	2.0538641794914883	2.458914936890708
5	1.4089663060655402	2.1085427228798412
6	1.12407795527215	2.0186427871286443
7	0.9190342749152234	1.9254912156169806
8	0.7921258330708767	1.8966882642816418
9	0.6812093411409831	1.8349950507026516
>10	5.615344250213279	30.179953858618063
>50	0.23271936286396988	4.871028661554286
>100	0.1691295065302785	8.933232539005708
>500	0.006764782833574334	1.3795407290882455
>1k	0.004852996380607675	2.8711454839648476
>5k	8.823629782923045E-4	1.825331951862981
>10k+	2.941209927641015E-4	6.57916187221643
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136114	5.943809052612365	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13359	0.5833591337691096	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	9591	0.41881858312594733	No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	7964	0.3477709515186158	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG	6577	0.28720360976116727	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCT	6566	0.28672326314304764	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	5632	0.2459374684772532	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT	5140	0.22445287428499314	No Hit
GCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGC	4148	0.18113434290547695	No Hit
GGGCCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC	4087	0.1784706025686317	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3948	0.1724007680305745	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3943	0.17218242865870192	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT	3679	0.1606541098238307	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCC	3141	0.1371607934103431	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	2772	0.12104734776614806	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTC	2365	0.10327452289572155	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2295	0.10021777168950569	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2294	0.10017410381513117	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29659220275168746	0.0
2	0.0	0.0	0.0	0.9545797338268386	0.0
3	0.0	0.0	0.0	1.6758420148706579	0.0
4	0.0	0.0	0.0	2.1579353479652736	0.0
5	0.0	0.0	0.0	4.506611971198417	0.0
6	0.0	0.0	0.0	5.742631155368987	0.0
7	0.0	0.0	0.0	8.696413513809746	0.0
8	0.0	0.0	0.0	12.437483979348588	0.0
9	0.0	0.0	0.0	16.05012722635199	0.0
10	0.0	0.0	0.0	17.958893683136296	0.0
11	0.0	0.0	0.0	19.31840561603799	0.0
12	0.0	0.0	0.0	20.18870635232202	0.0
13	0.0	0.0	0.0	20.718528672107976	0.0
14	0.0	0.0	0.0	21.03996789537876	0.0
15	0.0	0.0	0.0	21.66428749531116	0.0
16	0.0	0.0	0.0	22.299960742580936	0.0
17	0.0	0.0	0.0	23.179999414850485	0.0
18	0.0	0.0	0.0	23.693140606625377	0.0
19	0.0	0.0	0.0	24.281477878073183	0.0
20	0.0	0.0	0.0	24.90942191157867	0.0
21	0.0	0.0	0.0	25.61714715156639	0.0
22	0.0	0.0	0.0	26.208410170597286	0.0
23	0.0	0.0	0.0	26.761551135299232	0.0
24	4.366787437451228E-5	0.0	0.0	27.193164405616912	0.0
25	4.366787437451228E-5	0.0	0.0	27.627747091392056	0.0
26	4.366787437451228E-5	0.0	0.0	28.03171859723067	0.0
27	4.366787437451228E-5	0.0	0.0	28.47774226609194	0.0
28	4.366787437451228E-5	0.0	0.0	28.893809773132293	0.0
29	4.366787437451228E-5	0.0	0.0	29.35472418715527	0.0
30	4.366787437451228E-5	0.0	0.0	29.760922754586982	0.0
31	4.366787437451228E-5	0.0	0.0	30.198256516447724	0.0
32	4.366787437451228E-5	0.0	0.0	30.68131054277858	0.0
33	4.366787437451228E-5	0.0	0.0	31.181700715236115	0.0
34	4.366787437451228E-5	0.0	0.0	31.707592926328367	0.0
35	4.366787437451228E-5	0.0	0.0	32.2127865649671	0.0
36	4.366787437451228E-5	0.0	0.0	32.71160469394715	0.0
37	4.366787437451228E-5	0.0	0.0	33.46815061748558	0.0
38	4.366787437451228E-5	0.0	0.0	34.196661765675564	0.0
39	4.366787437451228E-5	0.0	0.0	34.990325382432324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	230	0.0	45.000004	30
CGATAGA	20	7.0347567E-4	45.0	9
CGTTTTT	32090	0.0	44.432064	1
ATAACGG	125	0.0	43.2	1
CTATCGG	75	0.0	42.0	1
TATACGG	135	0.0	41.666668	1
TACGGGT	65	0.0	41.53846	3
ACGGGTA	130	0.0	41.53846	4
ACTACGG	65	0.0	41.53846	1
CACGACG	235	0.0	41.17021	25
CTCGAGG	290	0.0	41.12069	1
CATATTA	335	0.0	40.97015	28
CACTTAC	3840	0.0	40.957027	36
CGCACTT	3835	0.0	40.717083	34
GTTTTTT	35475	0.0	40.687103	2
GCACTTA	3885	0.0	40.366795	35
TCACGAC	240	0.0	40.312496	24
ACTTACT	3830	0.0	40.30026	37
CCGCACT	3900	0.0	39.980766	33
ACCCGCA	3950	0.0	39.93038	31
>>END_MODULE