FastQCFastQC Report
Thu 26 May 2016
SRR1043300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043300_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2545278
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1239054.8680340615052655No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC130710.5135391890394684No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT91160.35815341192592715No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA89540.351788684772351No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT69440.2728189219409432Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC51170.20103894348672324No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT48550.19074537241118653No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG35890.14100620835916547No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT34550.13574155750373829No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31170.1224620650475115No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30550.12002618181589594No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT30230.11876895176086855No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC27170.10674668935966916No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC25740.10112844255126552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA206.3129066E-446.017
CGTTTTT296900.045.380261
CACGACG4650.043.0322625
CATGCGG3550.042.7605631
CGACGGT4650.042.53763627
CGGTCTA4650.042.53763630
TCGGCGT11700.042.0683754
TAGCATA10500.041.83809329
TCGATGG2200.041.818181
GGCGATA4900.041.7755137
GCACTTA34100.041.68328535
CGCACTT34400.041.5872134
TCACGAC5000.041.424
CCGCACT34650.041.35353533
CATATGC10650.041.2488332
CACTTAC34650.041.15440436
ACGTAGG2300.041.01
CGTTCGA1350.040.8888913
ACCCGCA35450.040.74471331
TATGCGG4750.040.6736831
Produced by FastQC (version 0.11.5)