FastQCFastQC Report
Thu 26 May 2016
SRR1043299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043299_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2496260
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180344.7284337368703575No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC127730.5116854814802946No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC88410.3541698380777643No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG76600.3068590611554886No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT69670.27909752990473746No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG50400.20190204546000817No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC48490.19425059889594834No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC33450.13400046469518398No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC31950.12799147524696947No Hit
GGGCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC31260.12522734010079078No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC30130.1207005680498025No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29120.11665451515467139No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT27450.10996450690232588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT275900.044.4780731
TAGTAGG3950.041.582281
GCACTTA33350.040.95202635
GCGATAT556.184564E-1140.9090928
CACAACG556.184564E-1140.90909211
CCGCACT33550.040.707933
CGCACTT33750.040.66666434
ACCCGCA34250.040.4014631
TCGGCGT11150.040.3587464
CACTTAC33900.040.35398536
CCCGCAC34350.040.34934632
CTTGCGG5800.040.344831
GTTTTTT313850.039.9960172
AACGCAA38800.039.7229417
CGCAAGC39050.039.699119
CTTACTG33850.039.68242338
CTTAATC43350.039.6539810
CAACGCA39100.039.5907916
AGGGACT50950.039.5682035
ACGCAAG39050.039.52624518