FastQCFastQC Report
Thu 26 May 2016
SRR1043298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043298_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences815580
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT538276.599843056475146No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT61150.7497731675617352TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT60720.7445008460236886TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT46320.5679393805635253TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC26150.3206307167905049TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT21800.26729444076608055TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG19720.24179111797739034No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC17280.21187375855219598TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC14980.18367296893008656TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC14230.17447705927070306TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT13750.16859167708869763No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT12770.15657568846710318TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC11970.14676671816376075TruSeq Adapter, Index 13 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC9190.1126805463596459TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTTG9180.11255793423085411TruSeq Adapter, Index 19 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG8620.10569165501851444No Hit
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC8570.10507859437455552TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT8490.10409769734422131No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC8230.10090978199563501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCA351.0190888E-746.0000044
GTTCGAT700.046.00000438
ACGTAGG700.046.0000041
ACCCGCA700.046.00000431
TTCGATT700.046.00000439
TCAACGG700.046.0000041
CCCGCAC700.046.00000432
TCACGAC1050.046.024
ACAACGG301.8607334E-646.01
CGGGTTC301.8607334E-646.05
ACTGCGG1050.046.01
AAGAGTA206.310371E-446.019
CGCGGTA206.310371E-446.037
GCGGTAA206.310371E-446.038
TAGACGG600.046.01
TGGGTGC551.8189894E-1246.05
AGCTTCG253.4162767E-546.010
ATTACGG405.6079443E-946.01
TGGCGGA206.310371E-446.02
ATAGCGG800.046.01
Produced by FastQC (version 0.11.5)