SRR1043297_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043297_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	794912
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49104	6.177287548810435	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	5911	0.7436043234974438	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	5785	0.7277535123384726	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	4703	0.5916378165130228	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	2513	0.3161356225594783	No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2039	0.2565063805804919	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC	1867	0.2348687653476108	No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1704	0.21436335091179903	No Hit
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1631	0.2051799444466809	No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1407	0.17700072460851013	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	1314	0.16530131637212672	No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1250	0.15725011070407793	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1148	0.14441850167062517	No Hit
TGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT	1103	0.13875749768527837	TruSeq Adapter, Index 19 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	899	0.11309427961837286	No Hit
TGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	892	0.11221367899843002	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	865	0.10881707660722194	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC	841	0.10579787448170364	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	811	0.10202387182480577	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	35	1.2113014E-7	45.000004	1
ATAGCGG	70	0.0	45.000004	1
GTCTATG	30	2.1646538E-6	45.000004	21
CGCGATA	30	2.1646538E-6	45.000004	10
GCCGATG	30	2.1646538E-6	45.000004	8
GTGCTCG	30	2.1646538E-6	45.000004	9
GGGCGTA	35	1.2113014E-7	45.000004	6
CGGGCGT	30	2.1646538E-6	45.000004	5
GCGATCC	30	2.1646538E-6	45.000004	8
GAACCCA	30	2.1646538E-6	45.000004	8
TTCGATT	35	1.2113014E-7	45.000004	39
CCGTTGG	35	1.2113014E-7	45.000004	1
TCTGCGG	80	0.0	45.0	1
ACAACGG	25	3.889716E-5	45.0	1
TATCAGG	25	3.889716E-5	45.0	1
GTACCTC	40	6.8102963E-9	45.0	9
GTCGATG	20	7.031973E-4	45.0	40
CGACGGT	90	0.0	45.0	27
CCATGCA	45	3.8380676E-10	45.0	10
GCACCGA	180	0.0	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content