##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043297_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	794912
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13456332273258	33.0	31.0	34.0	30.0	34.0
2	32.434317257759346	34.0	31.0	34.0	31.0	34.0
3	32.513444255464755	34.0	31.0	34.0	31.0	34.0
4	36.08909917072582	37.0	35.0	37.0	35.0	37.0
5	36.04176059739946	37.0	35.0	37.0	35.0	37.0
6	36.272815104061834	37.0	37.0	37.0	35.0	37.0
7	36.19537508554406	37.0	37.0	37.0	35.0	37.0
8	36.09944497000926	37.0	37.0	37.0	35.0	37.0
9	37.66820981441971	39.0	38.0	39.0	35.0	39.0
10	37.506862394831124	39.0	37.0	39.0	35.0	39.0
11	37.24742235618534	39.0	37.0	39.0	34.0	39.0
12	37.25299907411135	39.0	37.0	39.0	34.0	39.0
13	37.27420771104223	39.0	37.0	39.0	34.0	39.0
14	38.67114598848678	40.0	38.0	41.0	35.0	41.0
15	38.79812985588342	40.0	38.0	41.0	35.0	41.0
16	38.849458808019	40.0	38.0	41.0	35.0	41.0
17	38.79684166297653	40.0	38.0	41.0	35.0	41.0
18	38.57263319713377	40.0	38.0	41.0	35.0	41.0
19	38.327156968318505	40.0	37.0	41.0	35.0	41.0
20	38.06522482991828	40.0	35.0	41.0	34.0	41.0
21	38.01207177649853	40.0	35.0	41.0	34.0	41.0
22	37.96846191779719	40.0	35.0	41.0	34.0	41.0
23	37.932553540517695	40.0	35.0	41.0	34.0	41.0
24	37.84368080995129	40.0	35.0	41.0	34.0	41.0
25	37.8029995773117	40.0	35.0	41.0	34.0	41.0
26	37.77883589630047	40.0	35.0	41.0	34.0	41.0
27	37.75631893844853	40.0	35.0	41.0	34.0	41.0
28	37.66129709955316	40.0	35.0	41.0	34.0	41.0
29	37.58078755887444	40.0	35.0	41.0	34.0	41.0
30	37.4223171873113	39.0	35.0	41.0	33.0	41.0
31	37.27237731975363	39.0	35.0	41.0	33.0	41.0
32	36.938447274666885	39.0	35.0	41.0	32.0	41.0
33	36.72827306670424	39.0	35.0	41.0	32.0	41.0
34	36.39600358278652	39.0	35.0	41.0	31.0	41.0
35	36.21132653677388	39.0	35.0	41.0	30.0	41.0
36	36.17712778269796	39.0	35.0	41.0	30.0	41.0
37	36.11049776579043	39.0	35.0	41.0	30.0	41.0
38	36.12184241777706	39.0	35.0	41.0	30.0	41.0
39	36.10437130147739	39.0	35.0	41.0	30.0	41.0
40	36.08372876494505	39.0	35.0	41.0	30.0	41.0
41	36.06567267823357	39.0	35.0	41.0	30.0	41.0
42	35.94147150879594	39.0	35.0	41.0	30.0	41.0
43	35.821796626544824	38.0	35.0	40.0	30.0	41.0
44	35.7124021275311	38.0	35.0	40.0	29.0	41.0
45	35.68890896099191	38.0	35.0	40.0	29.0	41.0
46	35.60604192665352	38.0	35.0	40.0	28.0	41.0
47	35.46607800611891	38.0	35.0	40.0	28.0	41.0
48	35.48310379010507	38.0	35.0	40.0	27.0	41.0
49	35.40928429813614	38.0	35.0	40.0	27.0	41.0
50	35.312428293949516	38.0	35.0	40.0	27.0	41.0
51	34.925426965500584	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	0.0
15	5.0
16	16.0
17	50.0
18	92.0
19	220.0
20	442.0
21	777.0
22	1185.0
23	2114.0
24	3570.0
25	6895.0
26	10493.0
27	12313.0
28	12017.0
29	11925.0
30	12788.0
31	15042.0
32	18644.0
33	24389.0
34	43014.0
35	76386.0
36	78394.0
37	62954.0
38	111042.0
39	290118.0
40	25.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.02689102693128	20.257462461253574	25.298523610160622	27.417122901654523
2	32.61656636206272	20.121724165693813	26.557656696590314	20.704052775653153
3	21.31896360855038	21.60880701260014	38.19252948754076	18.879699891308725
4	22.244978060464554	24.160032808663097	35.34642828388551	18.248560846986837
5	19.849618574131476	29.595225635038847	30.958269594621797	19.596886196207883
6	72.51582565114127	1.4885924479690833	21.753980314802142	4.2416015860875165
7	73.13325248580975	1.4187733988164728	17.733786884585967	7.71418723078781
8	60.198738979912235	3.2945785193832773	26.68785978825329	9.818822712451189
9	33.02277484803349	18.844979066865264	29.269780805925688	18.862465279175556
10	25.48093373857735	19.735140493538907	42.51237872871462	12.271547039169116
11	22.577341894448693	16.451632381949196	40.41428485165653	20.556740871945575
12	18.03935529970613	19.77564812205628	43.90964031238678	18.27535626585081
13	13.194793889134898	23.35415744132684	42.54823175395516	20.90281691558311
14	11.461394468821705	23.06569783825128	43.962224749406225	21.51068294352079
15	11.99679964574695	19.068651624330744	47.662508554406024	21.27204017551628
16	12.803052413348897	25.591763616601586	38.798634314238555	22.806549655810958
17	14.230883418541925	22.50878084618172	41.031963286502155	22.228372448774202
18	16.494027011795016	29.206000161024114	38.4351978583793	15.864774968801578
19	15.987555855239322	23.77609093836802	35.78572219314842	24.450631013244234
20	13.083586610844975	30.642259772150883	35.075580693208806	21.19857292379534
21	14.29617366450626	24.453272815104064	36.08764240570025	25.16291111468942
22	12.012776256994485	27.296480616722352	32.0567559679562	28.633987158326956
23	11.440385854031641	23.62211162996659	42.924122418582186	22.01338009741959
24	17.138626665593172	22.102320760033813	38.20209029427157	22.556962280101445
25	12.356084698683627	26.62772734592005	37.98093373857735	23.035254216818966
26	14.674202930638863	23.58286200233485	36.05820518497645	25.68472988204984
27	19.521531943158486	24.736448814459965	36.59650376393865	19.145515478442896
28	14.04281228613985	25.489110744333964	32.78778028259732	27.68029668692887
29	15.89999899359929	30.281339318062876	31.1143875045288	22.704274183809027
30	18.588975886639027	24.143930397326997	29.65586127772634	27.61123243830764
31	16.36004991747514	29.869595628195324	29.030257437301238	24.7400970170283
32	12.730088361982206	31.29465903143996	27.709733907652673	28.265518698925163
33	10.948759107926412	26.96940038645787	33.6076194597641	28.474221045851618
34	11.568198744011916	28.18865484481301	29.800531379574092	30.442615031600983
35	13.667173221689948	28.564042309085785	31.81647779880037	25.952306670423898
36	12.660898313272412	25.836947989211385	32.24029829717	29.261855400346203
37	12.498112998671552	23.531409766112475	36.018200756813336	27.952276478402645
38	11.947863411295842	22.712702789742764	30.357196771466526	34.98223702749487
39	18.29963568294352	20.60743830763657	32.70173302202004	28.391192987399865
40	12.556232639587778	18.215601223783263	37.718514753834384	31.509651382794573
41	16.441568374864136	23.846916388229136	29.396587295197456	30.314927941709268
42	17.103528440884023	20.078071534962362	35.0143160500785	27.804083974075116
43	20.537619258483957	23.70212048629282	33.55415442212472	22.20610583309851
44	13.658241415401957	25.752536129785437	34.80787810474619	25.78134435006642
45	16.934327321766435	22.108359164284852	34.163152650859466	26.79416086308925
46	20.637001328448935	19.847731572803028	32.32332635562176	27.19194074312628
47	14.576204661648081	18.739055392294997	37.343253089650176	29.341486856406746
48	16.18443299384083	17.044905599613543	32.064807173624246	34.70585423292138
49	18.22478463024838	16.87545288031883	38.22599231109859	26.673770178334205
50	16.585357876091944	15.876348576949399	38.871598365605244	28.666695181353408
51	14.54412563906445	15.654940421078056	36.568198744011916	33.23273519584558
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	1265.5
2	2275.0
3	28240.5
4	54206.0
5	29985.0
6	5764.0
7	5436.5
8	5109.0
9	5276.0
10	5443.0
11	5547.0
12	5651.0
13	5473.0
14	5295.0
15	4970.5
16	4646.0
17	4161.0
18	3676.0
19	3342.0
20	3008.0
21	2838.5
22	2669.0
23	2621.0
24	2573.0
25	2797.0
26	3173.5
27	3326.0
28	3768.0
29	4210.0
30	4762.5
31	5315.0
32	6467.0
33	7619.0
34	8484.5
35	9350.0
36	11044.0
37	12738.0
38	14845.0
39	16952.0
40	20311.5
41	23671.0
42	30091.5
43	36512.0
44	45647.0
45	54782.0
46	73106.0
47	91430.0
48	112475.0
49	133520.0
50	128744.0
51	123968.0
52	97739.5
53	71511.0
54	53998.0
55	36485.0
56	28535.0
57	20585.0
58	17327.0
59	14069.0
60	11137.5
61	8206.0
62	6910.5
63	5615.0
64	4574.5
65	3534.0
66	2779.5
67	2025.0
68	1889.5
69	1754.0
70	1561.0
71	1368.0
72	1170.5
73	973.0
74	859.0
75	592.0
76	439.0
77	367.5
78	296.0
79	236.5
80	177.0
81	115.0
82	53.0
83	55.0
84	57.0
85	43.0
86	29.0
87	17.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	794912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.06798641392027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.27868578246719	23.49899900102091
2	11.993132550418615	7.691912233743384
3	4.588218477518357	4.414047834034699
4	2.329841600996544	2.9885331522937375
5	1.3741028524243355	2.2032355801436343
6	0.8999752656268005	1.731623675459072
7	0.6562146821716652	1.4730438458747181
8	0.4804236705464556	1.2324975792007558
9	0.371968666679695	1.073545753453965
>10	3.518278598118649	23.85860236564515
>50	0.3160972222775365	6.936404039413659
>100	0.17644475679580646	10.282116813487937
>500	0.011077249305566325	2.2785151575042057
>1k	0.004351776512901056	2.6234640836319305
>5k	7.912320932547375E-4	1.4838259065795765
>10k+	3.9561604662736874E-4	6.229632978512613
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49104	6.177287548810435	No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT	5911	0.7436043234974438	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	5785	0.7277535123384726	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	4703	0.5916378165130228	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	2513	0.3161356225594783	No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2039	0.2565063805804919	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC	1867	0.2348687653476108	No Hit
ACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1704	0.21436335091179903	No Hit
GCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1631	0.2051799444466809	No Hit
AGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1407	0.17700072460851013	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	1314	0.16530131637212672	No Hit
ACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	1250	0.15725011070407793	TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	1148	0.14441850167062517	No Hit
TGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT	1103	0.13875749768527837	TruSeq Adapter, Index 19 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	899	0.11309427961837286	No Hit
TGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG	892	0.11221367899843002	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	865	0.10881707660722194	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC	841	0.10579787448170364	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT	811	0.10202387182480577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7639839378446922	0.0
2	0.0	0.0	0.0	2.5517289964172134	0.0
3	0.0	0.0	0.0	4.2526719938810835	0.0
4	0.0	0.0	0.0	5.33455275552514	0.0
5	0.0	0.0	0.0	10.853402640795458	0.0
6	0.0	0.0	0.0	12.204369791876333	0.0
7	0.0	0.0	0.0	16.8026146290407	0.0
8	0.0	0.0	0.0	22.33769272573568	0.0
9	0.0	0.0	0.0	26.582816714302968	0.0
10	0.0	0.0	0.0	29.089886679280223	0.0
11	0.0	0.0	0.0	31.33906646270279	0.0
12	0.0	0.0	0.0	32.909428968238	0.0
13	0.0	0.0	0.0	33.56975363310656	0.0
14	0.0	0.0	0.0	34.052951773278046	0.0
15	0.0	0.0	0.0	35.58783865383841	0.0
16	0.0	0.0	0.0	37.281107845899925	0.0
17	0.0	0.0	0.0	39.25340163439475	0.0
18	1.2580008856326235E-4	0.0	0.0	40.250241536170044	0.0
19	1.2580008856326235E-4	0.0	0.0	41.35665331508393	0.0
20	1.2580008856326235E-4	0.0	0.0	42.56873716839097	0.0
21	5.032003542530494E-4	0.0	0.0	43.70395716758585	0.0
22	5.032003542530494E-4	0.0	0.0	44.83968036713498	0.0
23	6.290004428163117E-4	0.0	0.0	45.722671188760515	0.0
24	0.0026418018598285092	0.0	0.0	46.43256108852301	0.0
25	0.0026418018598285092	0.0	0.0	47.11049776579043	0.0
26	0.0026418018598285092	0.0	0.0	47.79346644660038	0.0
27	0.0026418018598285092	0.0	0.0	48.45240731049475	0.0
28	0.0026418018598285092	0.0	0.0	49.146320599009705	0.0
29	0.0026418018598285092	0.0	0.0	49.931942152087274	0.0
30	0.0026418018598285092	0.0	0.0	50.63264864538465	0.0
31	0.0026418018598285092	0.0	0.0	51.227305664023184	0.0
32	0.0026418018598285092	0.0	0.0	51.821836882573166	0.0
33	0.0026418018598285092	0.0	0.0	52.44656012237833	0.0
34	0.0026418018598285092	0.0	0.0	53.119338996014655	0.0
35	0.0026418018598285092	0.0	0.0	53.68569099472646	0.0
36	0.0026418018598285092	0.0	0.0	54.19052675013083	0.0
37	0.003019202125518296	0.0	0.0	54.717377521033775	0.0
38	0.003019202125518296	0.0	0.0	55.23227728352321	0.0
39	0.003019202125518296	0.0	0.0	55.75019624813816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCGG	35	1.2113014E-7	45.000004	1
ATAGCGG	70	0.0	45.000004	1
GTCTATG	30	2.1646538E-6	45.000004	21
CGCGATA	30	2.1646538E-6	45.000004	10
GCCGATG	30	2.1646538E-6	45.000004	8
GTGCTCG	30	2.1646538E-6	45.000004	9
GGGCGTA	35	1.2113014E-7	45.000004	6
CGGGCGT	30	2.1646538E-6	45.000004	5
GCGATCC	30	2.1646538E-6	45.000004	8
GAACCCA	30	2.1646538E-6	45.000004	8
TTCGATT	35	1.2113014E-7	45.000004	39
CCGTTGG	35	1.2113014E-7	45.000004	1
TCTGCGG	80	0.0	45.0	1
ACAACGG	25	3.889716E-5	45.0	1
TATCAGG	25	3.889716E-5	45.0	1
GTACCTC	40	6.8102963E-9	45.0	9
GTCGATG	20	7.031973E-4	45.0	40
CGACGGT	90	0.0	45.0	27
CCATGCA	45	3.8380676E-10	45.0	10
GCACCGA	180	0.0	45.0	8
>>END_MODULE