##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043296_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2185999
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31847864523268	33.0	31.0	34.0	31.0	34.0
2	32.65793945925867	34.0	31.0	34.0	31.0	34.0
3	32.77526018996349	34.0	31.0	34.0	31.0	34.0
4	36.21974483977348	37.0	37.0	37.0	35.0	37.0
5	36.15307921000879	37.0	37.0	37.0	35.0	37.0
6	36.310940215434684	37.0	37.0	37.0	35.0	37.0
7	36.24740541967311	37.0	37.0	37.0	35.0	37.0
8	36.22569497973238	37.0	37.0	37.0	35.0	37.0
9	37.8149198604391	39.0	38.0	39.0	35.0	39.0
10	37.616074389787	39.0	37.0	39.0	35.0	39.0
11	37.22539992012805	39.0	37.0	39.0	35.0	39.0
12	37.2212361487814	39.0	37.0	39.0	34.0	39.0
13	37.191845009993145	39.0	37.0	39.0	34.0	39.0
14	38.48571339694117	40.0	37.0	41.0	34.0	41.0
15	38.54078570026793	40.0	37.0	41.0	35.0	41.0
16	38.39562689644414	40.0	37.0	41.0	34.0	41.0
17	38.35399604482893	40.0	37.0	41.0	34.0	41.0
18	38.14538661728574	40.0	37.0	41.0	34.0	41.0
19	37.932784049763974	40.0	37.0	41.0	34.0	41.0
20	37.63684750084515	39.0	35.0	41.0	34.0	41.0
21	37.56705423927458	39.0	35.0	41.0	34.0	41.0
22	37.51981817009065	39.0	35.0	41.0	34.0	41.0
23	37.52742476094454	39.0	35.0	41.0	34.0	41.0
24	37.45118639121061	39.0	35.0	41.0	34.0	41.0
25	37.34163007393874	39.0	35.0	41.0	33.0	41.0
26	37.24073707261531	39.0	35.0	41.0	33.0	41.0
27	37.27175721489351	39.0	35.0	41.0	33.0	41.0
28	37.23252389410974	39.0	35.0	41.0	33.0	41.0
29	37.14943922664192	39.0	35.0	41.0	33.0	41.0
30	37.08413132851388	39.0	35.0	41.0	33.0	41.0
31	36.95327353763657	39.0	35.0	41.0	33.0	41.0
32	36.66388456719331	39.0	35.0	41.0	32.0	41.0
33	36.27583223963048	39.0	35.0	41.0	31.0	41.0
34	35.97660291701872	38.0	35.0	41.0	30.0	41.0
35	35.82494411022146	38.0	35.0	41.0	30.0	41.0
36	35.772159090649176	38.0	35.0	41.0	30.0	41.0
37	35.670376793402006	38.0	35.0	41.0	29.0	41.0
38	35.55679714400601	38.0	35.0	41.0	29.0	41.0
39	35.52217087016051	38.0	35.0	41.0	29.0	41.0
40	35.45418090310197	38.0	35.0	40.0	29.0	41.0
41	35.40804273012019	37.0	35.0	40.0	29.0	41.0
42	35.33275038094711	37.0	35.0	40.0	28.0	41.0
43	35.23077915406183	37.0	35.0	40.0	28.0	41.0
44	35.05043369187268	37.0	35.0	40.0	27.0	41.0
45	34.961235572385895	37.0	35.0	40.0	26.0	41.0
46	34.921642233139174	36.0	35.0	40.0	26.0	41.0
47	34.84404338702808	36.0	35.0	40.0	26.0	41.0
48	34.7418672195184	36.0	35.0	40.0	25.0	41.0
49	34.6818429468632	36.0	35.0	40.0	26.0	41.0
50	34.573157169788274	35.0	35.0	40.0	26.0	41.0
51	34.45181859644035	35.0	34.0	40.0	25.0	41.0
52	34.04430285649719	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	15.0
14	13.0
15	41.0
16	118.0
17	223.0
18	498.0
19	999.0
20	1804.0
21	3072.0
22	4557.0
23	7117.0
24	12084.0
25	21333.0
26	31960.0
27	38631.0
28	39907.0
29	39930.0
30	41687.0
31	46577.0
32	54200.0
33	70283.0
34	164500.0
35	223622.0
36	206287.0
37	203447.0
38	300258.0
39	671354.0
40	1475.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.88363626881805	16.961261189963945	23.2267260872489	30.928376453969104
2	35.77755525048273	19.0343179479954	23.266158859176056	21.92196794234581
3	25.287157038955645	20.563412883537456	34.56236713740491	19.587062940101983
4	22.88267286490067	23.202755353501992	32.537206101192176	21.377365680405163
5	21.144977650950437	31.12384772362659	29.647314568762383	18.083860056660594
6	77.36815067161513	1.9977593768341158	17.85705299956679	2.777036951983967
7	77.0995778131646	1.891995376027162	16.532486977349944	4.475939833458295
8	68.0805434952166	3.279873412567892	21.671007168804742	6.968575923410761
9	42.320650649885934	14.3917723658611	26.016617573933015	17.27095941031995
10	29.884048437350614	15.540080301958051	34.83624649416582	19.73962476652551
11	26.773571259639183	12.332256327656141	39.38583686451824	21.508335548186437
12	22.264328574715726	13.712174616731298	41.347182683981096	22.676314124571878
13	17.534820464236258	16.39392332750381	43.832087754843435	22.239168453416493
14	17.914875532879933	16.523246350981864	43.55665304512948	22.005225071008724
15	22.063184841347137	14.025120780018655	42.53428295255396	21.37741142608025
16	20.922150467589418	17.890218614006688	39.13130792832018	22.05632299008371
17	21.5172102091538	18.9649675045597	38.914061717320095	20.603760568966408
18	21.406963132188075	24.980020576404655	35.22311766839783	18.389898623009433
19	23.3165705931247	21.391089382932012	33.48679482470028	21.805545199243
20	21.910028321147447	25.644979709505815	33.93985999078682	18.505131978559916
21	19.53468414212449	19.965791384168064	36.12202018390676	24.37750428980068
22	21.329378467236264	21.38486797111984	33.9316715149458	23.3540820466981
23	22.373020298728406	19.11734634828287	36.48505786141713	22.02457549157159
24	24.369132831259304	17.69346646544669	33.29617259660228	24.641228106691724
25	18.808425804403388	18.34799558462744	36.87837917583677	25.9651994351324
26	17.377729815978874	18.054445587578037	36.83688784853058	27.73093674791251
27	21.32009209519309	21.01025663781182	35.336887162345455	22.332764104649634
28	23.474667646234057	21.880522360714714	31.344067403507502	23.300742589543727
29	22.729790818751518	18.956001352242154	33.902257045863244	24.411950783143084
30	26.342784237321244	19.819633952257067	30.624396442999284	23.2131853674224
31	28.35005871457398	17.996302834539264	29.411312630975583	24.24232581991117
32	25.120505544604548	19.578005296434263	28.797268434249055	26.504220724712134
33	24.217302935637207	20.973888826115658	32.01241171656529	22.79639652168185
34	23.004356360638774	22.571693765642163	31.611771094131335	22.81217877958773
35	25.215748040140916	25.38701984767605	28.132309301147895	21.264922811035138
36	27.56675552001625	22.34273666181915	27.927643150797415	22.162864667367185
37	25.104952015074115	25.885144503725755	29.572977846741928	19.436925634458206
38	24.656049705420724	22.133724672335163	29.29937296403155	23.910852658212562
39	25.624394155715535	20.237200474474143	32.19896257958032	21.939442790230004
40	21.786194778680137	20.05403479141573	34.182952508212495	23.976817921691637
41	21.98564592207041	22.90970855887857	29.730754680125653	25.373890838925362
42	21.296167107121274	18.313686328310304	32.155778662295816	28.2343679022726
43	23.93152970335302	18.571371716089534	32.48793800912077	25.009160571436674
44	22.807970177479493	19.909386966782694	32.932951936391554	24.34969091934626
45	23.443240367447558	21.015471644772024	31.969227799280787	23.57206018849963
46	23.15870226839079	20.258426467715676	31.94658369011148	24.636287573782056
47	21.30979931829795	20.747036023346762	33.46657523631072	24.47658942204457
48	23.07128228329473	18.67846234147408	31.035787299079274	27.21446807615191
49	22.05833579978765	17.214509247259492	34.97023557650301	25.75691937644985
50	21.210851423079333	17.42676917967483	36.16451791606492	25.197861481180915
51	19.95092403976397	16.195387097615324	35.78976934573163	28.063919516889076
52	17.76199348673078	17.29639400566972	38.75591891853564	26.185693589063856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	521.0
1	3332.0
2	6143.0
3	73790.5
4	141438.0
5	80523.0
6	19608.0
7	17731.0
8	15854.0
9	15572.5
10	15291.0
11	14667.0
12	14043.0
13	13369.5
14	11871.0
15	11046.0
16	10311.0
17	9576.0
18	8971.0
19	8366.0
20	7920.0
21	7474.0
22	7278.5
23	7083.0
24	6871.0
25	6659.0
26	7433.0
27	8207.0
28	8502.5
29	8798.0
30	9307.0
31	9816.0
32	12066.5
33	14317.0
34	16172.5
35	18028.0
36	19583.5
37	21139.0
38	23815.0
39	28131.0
40	29771.0
41	34737.0
42	39703.0
43	47771.0
44	55839.0
45	65856.5
46	75874.0
47	94717.0
48	113560.0
49	148668.0
50	183776.0
51	204000.0
52	224224.0
53	214384.5
54	204545.0
55	186667.5
56	168790.0
57	152429.5
58	136069.0
59	126861.0
60	117653.0
61	115087.0
62	112521.0
63	109425.0
64	90912.5
65	75496.0
66	63193.5
67	50891.0
68	41452.0
69	32013.0
70	28288.0
71	24563.0
72	23585.5
73	22608.0
74	18295.0
75	13982.0
76	10655.0
77	7328.0
78	5629.5
79	3931.0
80	2971.0
81	2011.0
82	1511.5
83	1012.0
84	769.5
85	527.0
86	396.5
87	266.0
88	163.0
89	48.5
90	37.0
91	25.0
92	13.0
93	10.5
94	8.0
95	5.0
96	2.0
97	1.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2185999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.96598348480951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.80641979996791	22.416479402835655
2	9.92409033955714	5.947702544338536
3	3.5553719394376495	3.1962065045863124
4	1.837072902403599	2.2019878501526926
5	1.113304390206541	1.6680630485247563
6	0.8544426539515676	1.536252867421769
7	0.6629424829088442	1.3906006445957524
8	0.565337511722367	1.3552715631692602
9	0.5038567709860561	1.358870731026984
>10	5.736053097932955	35.65883660146678
>50	0.29494741787143003	5.908370256333878
>100	0.13481174603838417	6.940774939975112
>500	0.006441294252082859	1.3495754796833563
>1k	0.004140832019196123	2.598872620584819
>5k	4.6009244657734704E-4	1.0191441067036064
>10k+	3.0672829771823134E-4	5.452990838600727
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	105701	4.835363602636598	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12953	0.59254372943446	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	8840	0.4043917677912936	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	7219	0.33023802847119327	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCTT	6117	0.2798262945225501	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	4615	0.21111629053810183	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	3725	0.17040263970843536	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	3469	0.15869174688552007	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3168	0.1449222986835767	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3042	0.1391583436222981	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	2995	0.137008296893091	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCG	2711	0.12401652516766933	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCT	2407	0.11010983994045744	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2350	0.1075023364603552	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2285	0.10452886757953686	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	2215	0.10132667032327096	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.574567508951285E-5	0.0	0.0	0.2887467011650051	0.0
2	4.574567508951285E-5	0.0	0.0	0.9354990555805378	0.0
3	4.574567508951285E-5	0.0	0.0	1.5835322888985768	0.0
4	4.574567508951285E-5	0.0	0.0	2.039296449815393	0.0
5	4.574567508951285E-5	0.0	0.0	4.217934225953443	0.0
6	4.574567508951285E-5	0.0	0.0	5.10745887806902	0.0
7	4.574567508951285E-5	0.0	0.0	7.530561541885426	0.0
8	4.574567508951285E-5	0.0	0.0	10.427543653954096	0.0
9	4.574567508951285E-5	0.0	0.0	13.262311647901027	0.0
10	4.574567508951285E-5	0.0	0.0	14.794563035024261	0.0
11	4.574567508951285E-5	0.0	0.0	15.980885627120598	0.0
12	9.14913501790257E-5	0.0	0.0	16.790492584854796	0.0
13	9.14913501790257E-5	0.0	0.0	17.237336339129158	0.0
14	9.14913501790257E-5	0.0	0.0	17.52567132921836	0.0
15	9.14913501790257E-5	0.0	0.0	18.20517758699798	0.0
16	9.14913501790257E-5	0.0	0.0	18.930612502567477	0.0
17	9.14913501790257E-5	0.0	0.0	19.8373832741918	0.0
18	9.14913501790257E-5	0.0	0.0	20.377136494572962	0.0
19	9.14913501790257E-5	0.0	0.0	20.97183027073663	0.0
20	9.14913501790257E-5	0.0	0.0	21.62274548158531	0.0
21	9.14913501790257E-5	0.0	0.0	22.290678083567283	0.0
22	1.3723702526853855E-4	0.0	0.0	22.914145889362256	0.0
23	1.3723702526853855E-4	0.0	0.0	23.476451727562548	0.0
24	1.829827003580514E-4	0.0	0.0	23.933816987107498	0.0
25	1.829827003580514E-4	0.0	0.0	24.39927923114329	0.0
26	1.829827003580514E-4	0.0	0.0	24.85646150798788	0.0
27	1.829827003580514E-4	0.0	0.0	25.328602620586743	0.0
28	1.829827003580514E-4	0.0	0.0	25.78628809985732	0.0
29	1.829827003580514E-4	0.0	0.0	26.271009273105797	0.0
30	1.829827003580514E-4	0.0	0.0	26.713324205546296	0.0
31	1.829827003580514E-4	0.0	0.0	27.17709385960378	0.0
32	1.829827003580514E-4	0.0	0.0	27.69992118020182	0.0
33	1.829827003580514E-4	0.0	0.0	28.212044012828915	0.0
34	1.829827003580514E-4	0.0	0.0	28.744523670870848	0.0
35	1.829827003580514E-4	0.0	0.0	29.217305222920963	0.0
36	1.829827003580514E-4	0.0	0.0	29.71808312812586	0.0
37	1.829827003580514E-4	0.0	0.0	30.36039815205771	0.0
38	1.829827003580514E-4	0.0	0.0	31.031395714270683	0.0
39	1.829827003580514E-4	0.0	0.0	31.788349399976852	0.0
40	1.829827003580514E-4	0.0	0.0	32.45605327358338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTT	20	6.3127093E-4	46.000004	32
TACGTTA	20	6.3127093E-4	46.000004	35
TACCGGA	20	6.3127093E-4	46.000004	34
AAGTCGA	20	6.3127093E-4	46.000004	27
CGTTTTT	26490	0.0	45.296715	1
TATACGG	90	0.0	43.444447	1
AATGCGG	265	0.0	41.660378	1
CACTTAC	3085	0.0	41.45219	36
ACTTACT	3070	0.0	41.429966	37
ATAGCGG	285	0.0	41.157898	1
CATGCGG	305	0.0	40.72131	1
TAGCATA	1175	0.0	40.714893	29
TAAACGG	85	0.0	40.588234	1
GTTTTTT	30095	0.0	40.51271	2
GGCGATA	335	0.0	40.50746	7
GCACTTA	3165	0.0	40.477093	35
CGCACTT	3175	0.0	40.349606	34
ACAACGG	200	0.0	40.25	1
TACGCGG	200	0.0	40.25	1
ACCCGCA	3190	0.0	40.231976	31
>>END_MODULE