FastQCFastQC Report
Thu 26 May 2016
SRR1043295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043295_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2136694
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1005664.706616857631463No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC123770.5792593604886802No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG73610.3445041732695463No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC69010.3229755875197853No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT58650.27448946830945375No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG45680.2137882167498013No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC37220.17419433947958857No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC31820.14892165186030382No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC30090.1408250315674589No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC28760.13460046220937577No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT27450.1284694953980308No Hit
GGGCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC24310.11377389556015041No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC24190.11321228027972184No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA21720.10165236575756754No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT21590.10104394920376994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT253050.044.3775941
CTAGCGG5650.042.6106221
TAGTAGG4950.041.3636361
CATGCGG3550.041.1971861
CACTTAC34200.041.1842136
CCGGTAT1050.040.71428742
TCGATTA1550.040.64516440
ACTTACT34450.040.62409237
CGCACTT35100.040.57692334
CTTACGG2000.040.51
GCACTTA34950.040.4935635
CCAGTAG10900.040.45871425
CCGCACT35300.040.34702733
ACCCGCA35650.040.2664831
CCCGCAC35950.040.24339332
TGTACGG2350.040.2127651
TACGGGT1400.040.178573
AATGCGG2800.040.178571
TAGCATA10450.040.04784829
AGGGCGA8150.040.0306745