##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043295_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2136694
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30647860666993	33.0	31.0	34.0	31.0	34.0
2	32.605140464661766	34.0	31.0	34.0	31.0	34.0
3	32.69648250989613	34.0	31.0	34.0	31.0	34.0
4	36.19263731727613	37.0	37.0	37.0	35.0	37.0
5	36.17435673989818	37.0	37.0	37.0	35.0	37.0
6	36.33367623066288	37.0	37.0	37.0	35.0	37.0
7	36.28153352796423	37.0	37.0	37.0	35.0	37.0
8	36.18505176688847	37.0	37.0	37.0	35.0	37.0
9	37.78072761003681	39.0	38.0	39.0	35.0	39.0
10	37.567715826412204	39.0	37.0	39.0	35.0	39.0
11	37.22209731482374	39.0	37.0	39.0	35.0	39.0
12	37.22821751734221	39.0	37.0	39.0	34.0	39.0
13	37.207848667146536	39.0	37.0	39.0	34.0	39.0
14	38.49882996816577	40.0	37.0	41.0	34.0	41.0
15	38.56549042586351	40.0	37.0	41.0	35.0	41.0
16	38.4592449831375	40.0	37.0	41.0	34.0	41.0
17	38.39379246630542	40.0	37.0	41.0	34.0	41.0
18	38.20064969527691	40.0	37.0	41.0	34.0	41.0
19	38.01055368714472	40.0	37.0	41.0	34.0	41.0
20	37.73568185243184	40.0	35.0	41.0	34.0	41.0
21	37.67183836337819	39.0	35.0	41.0	34.0	41.0
22	37.64345105101619	39.0	35.0	41.0	34.0	41.0
23	37.6153679469311	39.0	35.0	41.0	34.0	41.0
24	37.54168168207521	39.0	35.0	41.0	34.0	41.0
25	37.49926756007177	39.0	35.0	41.0	34.0	41.0
26	37.45983374315649	39.0	35.0	41.0	34.0	41.0
27	37.43902683304207	39.0	35.0	41.0	34.0	41.0
28	37.35250906306659	39.0	35.0	41.0	34.0	41.0
29	37.295761115068416	39.0	35.0	41.0	33.0	41.0
30	37.177452176118805	39.0	35.0	41.0	33.0	41.0
31	37.03376711873577	39.0	35.0	41.0	33.0	41.0
32	36.755095488638055	39.0	35.0	41.0	33.0	41.0
33	36.36203312219719	39.0	35.0	41.0	31.0	41.0
34	36.02561995306768	38.0	35.0	41.0	30.0	41.0
35	35.87054206170841	38.0	35.0	41.0	30.0	41.0
36	35.80408425352437	38.0	35.0	41.0	30.0	41.0
37	35.66725090256256	38.0	35.0	41.0	29.0	41.0
38	35.552645816387376	38.0	35.0	41.0	29.0	41.0
39	35.472951672069094	38.0	35.0	40.0	29.0	41.0
40	35.382195578777306	38.0	35.0	40.0	29.0	41.0
41	35.28888694403597	37.0	35.0	40.0	28.0	41.0
42	35.19225822696184	37.0	35.0	40.0	27.0	41.0
43	35.07558031238914	37.0	35.0	40.0	27.0	41.0
44	34.96360732982823	37.0	35.0	40.0	26.0	41.0
45	34.90116647493745	36.0	35.0	40.0	26.0	41.0
46	34.80165339538558	36.0	35.0	40.0	25.0	41.0
47	34.66350960876944	36.0	35.0	40.0	25.0	41.0
48	34.5885503492779	36.0	35.0	40.0	24.0	41.0
49	34.513266756961926	35.0	34.0	40.0	25.0	41.0
50	34.43288791001426	35.0	34.0	40.0	25.0	41.0
51	34.00111106222978	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	8.0
14	18.0
15	40.0
16	95.0
17	181.0
18	444.0
19	867.0
20	1554.0
21	2666.0
22	4057.0
23	6213.0
24	10186.0
25	18284.0
26	29133.0
27	36975.0
28	39526.0
29	40378.0
30	41351.0
31	45142.0
32	52338.0
33	67774.0
34	159872.0
35	221652.0
36	195111.0
37	200677.0
38	310606.0
39	651392.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.13351186459081	17.11990579839696	23.249328167720787	30.497254169291438
2	35.441995905824605	19.107087865646648	23.390153199288246	22.060763029240498
3	25.246291701104607	20.739095069298646	34.29812598341176	19.716487246184993
4	23.15801888337778	23.30988901546033	32.11891829152888	21.41317380963301
5	21.33286282453173	31.241628422226114	29.23872112712443	18.18678762611773
6	77.73059689408029	1.9709888266639959	17.521647928996853	2.7767663502588578
7	77.46467205879738	1.8508499579256554	16.189964496554023	4.494513486722947
8	68.46361715809563	3.1985862271340677	21.358135512151012	6.97966110261928
9	42.58284059392688	14.400705014382032	25.64471094129529	17.371743450395797
10	30.152609592201784	15.509614385588202	34.53845052216181	19.799325500048205
11	27.032930311967927	12.317393131632326	39.134850381009166	21.51482617539058
12	22.495687262659043	13.658483620022333	41.1701909585556	22.67563815876302
13	17.616046097382217	16.335469655458386	43.730688624576096	22.3177956225833
14	18.021485528578264	16.483595685671414	43.44080153732823	22.054117248422095
15	22.205659771591066	14.00097533853701	42.380050676418804	21.41331421345312
16	21.082990826014395	17.82337573840709	39.02510139495875	22.06853204061976
17	21.757443976535715	18.86760574981724	38.693795180779276	20.68115509286777
18	21.51384334864983	25.136027901047132	34.99892825083985	18.35120049946319
19	23.48707863643554	21.466433658726984	33.186502138350185	21.859985566487293
20	21.933931578410384	25.827329509981308	33.65512328859444	18.58361562301387
21	19.613898854960045	20.05940017615999	35.84626530518643	24.480435663693537
22	21.462455550490617	21.42950745403881	33.592549986100025	23.51548700937055
23	22.519555912077255	19.14181441048648	36.170036514353484	22.168593163082782
24	24.476551158003907	17.64684133525905	33.12959178993342	24.747015716803624
25	18.805594062603255	18.43216670239164	36.7448497538721	26.017389481133
26	17.46876249008983	17.952219643992073	36.62134119345119	27.957676672466903
27	21.395950941033202	21.009746833191837	35.13048663028024	22.463815595494722
28	23.612459247791215	21.88848754196904	31.063221968143313	23.435831242096437
29	22.849645293149138	18.95301807371575	33.5764035467877	24.620933086347414
30	26.435558858685425	19.788888816086907	30.330594834824264	23.444957490403397
31	28.674578577933946	17.912906574362076	29.027787788050137	24.384727059653837
32	25.29463741649483	19.50967241916718	28.503051911036398	26.6926382533016
33	24.390811225191815	20.894568899430617	31.771512439310452	22.94310743606712
34	23.182917160810113	22.529524583304863	31.41923925466164	22.868319001223387
35	25.380658156947135	25.397693820453465	27.856164710529445	21.365483312069955
36	27.715526884055464	22.300806760350337	27.740425161487792	22.243241194106407
37	25.252890680649635	25.741776782262694	29.468842988280024	19.536489548807644
38	24.8829734159407	22.04344655809395	29.133465063317445	23.940114962647904
39	25.828078330355215	20.19442184982969	32.00631442780295	21.971185392012146
40	21.997768515285763	20.000477372988364	33.946508016590116	24.055246095135757
41	22.098391253029213	22.83920860918784	29.57292902025278	25.489471117530165
42	21.517025835238925	18.24674005730348	31.984458233139605	28.251775874317985
43	24.195696716516263	18.433055926585652	32.288666510038404	25.08258084685968
44	22.86462170062723	19.719950540414306	32.91566316936351	24.499764589594957
45	23.51398936862274	20.909451704361974	31.972851517344086	23.603707409671202
46	23.348921277450117	20.156840427314346	31.859171224330673	24.635067070904864
47	21.504295888882545	20.50106379294368	33.425422638899164	24.56921767927462
48	23.257705595653846	18.51551977026191	30.885470731887672	27.34130390219657
49	22.148234609167247	17.063697469080736	34.82347027697929	25.96459764477272
50	21.352191750433146	17.29854625884661	36.12150359386978	25.227758396850465
51	20.09932166234379	16.2091998199087	35.50962374584288	28.181854771904636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	513.0
1	3198.0
2	5883.0
3	70131.5
4	134380.0
5	77265.5
6	20151.0
7	17834.5
8	15518.0
9	15076.5
10	14635.0
11	14087.0
12	13539.0
13	12784.0
14	12029.0
15	11068.0
16	10107.0
17	9582.0
18	9057.0
19	8342.5
20	7628.0
21	7230.0
22	6832.0
23	6732.0
24	6632.0
25	6409.5
26	6992.5
27	7798.0
28	8089.5
29	8381.0
30	9453.5
31	10526.0
32	12466.5
33	14407.0
34	16704.5
35	19002.0
36	21139.0
37	23276.0
38	25040.0
39	26804.0
40	30102.5
41	33401.0
42	39978.5
43	46556.0
44	53425.5
45	60295.0
46	70188.0
47	80081.0
48	110502.0
49	140923.0
50	175234.0
51	209545.0
52	219739.0
53	229933.0
54	209450.0
55	188967.0
56	169982.5
57	150998.0
58	137758.0
59	124518.0
60	120326.5
61	116135.0
62	109899.0
63	103663.0
64	99195.5
65	94728.0
66	77994.5
67	61261.0
68	49772.0
69	38283.0
70	32355.0
71	26427.0
72	24732.5
73	23038.0
74	19727.0
75	12541.5
76	8667.0
77	6719.5
78	4772.0
79	3589.5
80	2407.0
81	1812.0
82	1217.0
83	960.0
84	703.0
85	503.5
86	304.0
87	198.5
88	93.0
89	72.0
90	51.0
91	33.0
92	15.0
93	12.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2136694.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.74909479786696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30152861242368	22.104032183174166
2	10.04435917117595	5.976211863342755
3	3.664249749823961	3.270243395117199
4	1.8247399262882935	2.1713744419441285
5	1.2143271938599798	1.806256740288415
6	0.8576966278595926	1.5309418974003504
7	0.6947655657847476	1.446805267517689
8	0.5870127222442285	1.3970477697278
9	0.5163694441402217	1.3825371190004387
>10	5.854715802241022	35.862455707055446
>50	0.287343240734104	5.706320472661297
>100	0.1413022773386398	7.1717736423971585
>500	0.006509264513894203	1.3284028278252582
>1k	0.004286588826223012	2.560644518003776
>5k	4.762876473581124E-4	0.9506066883182455
>10k+	3.1752509823874167E-4	5.334345466225845
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100566	4.706616857631463	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12377	0.5792593604886802	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7361	0.3445041732695463	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	6901	0.3229755875197853	No Hit
CTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGCT	5865	0.27448946830945375	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTG	4568	0.2137882167498013	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTCTGC	3722	0.17419433947958857	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	3182	0.14892165186030382	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3009	0.1408250315674589	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2876	0.13460046220937577	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTT	2745	0.1284694953980308	No Hit
GGGCCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCTTC	2431	0.11377389556015041	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCC	2419	0.11321228027972184	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2172	0.10165236575756754	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCGTAGTCGTATGCCGTCT	2159	0.10104394920376994	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28399012680337005	0.0
2	0.0	0.0	0.0	0.9259631936065716	0.0
3	0.0	0.0	0.0	1.5801513927590942	0.0
4	0.0	0.0	0.0	2.0443732233066596	0.0
5	0.0	0.0	0.0	4.268042124890134	0.0
6	0.0	0.0	0.0	5.167562599043195	0.0
7	0.0	0.0	0.0	7.633100481397898	0.0
8	0.0	0.0	0.0	10.594731861464487	0.0
9	0.0	0.0	0.0	13.523134337439053	0.0
10	0.0	0.0	0.0	15.10122647416991	0.0
11	0.0	0.0	0.0	16.29943267496422	0.0
12	0.0	0.0	0.0	17.13141891164575	0.0
13	0.0	0.0	0.0	17.58047712962174	0.0
14	0.0	0.0	0.0	17.88229854157872	0.0
15	0.0	0.0	0.0	18.578935495676966	0.0
16	0.0	0.0	0.0	19.33412084275989	0.0
17	0.0	0.0	0.0	20.27524764893803	0.0
18	0.0	0.0	0.0	20.825771027578117	0.0
19	0.0	0.0	0.0	21.43339195972844	0.0
20	0.0	0.0	0.0	22.10035690651071	0.0
21	0.0	0.0	0.0	22.78477872825964	0.0
22	0.0	0.0	0.0	23.416174707281435	0.0
23	0.0	0.0	0.0	23.999318573459746	0.0
24	0.0	0.0	0.0	24.4705137937393	0.0
25	0.0	0.0	0.0	24.934127207733066	0.0
26	0.0	0.0	0.0	25.398770249740956	0.0
27	0.0	0.0	0.0	25.887188338620316	0.0
28	0.0	0.0	0.0	26.35899197545367	0.0
29	0.0	0.0	0.0	26.85073295474223	0.0
30	0.0	0.0	0.0	27.30405008859481	0.0
31	0.0	0.0	0.0	27.77547931524121	0.0
32	0.0	0.0	0.0	28.31097948512983	0.0
33	4.6801273369045826E-5	0.0	0.0	28.828648369864847	0.0
34	4.6801273369045826E-5	0.0	0.0	29.367799039076257	0.0
35	4.6801273369045826E-5	0.0	0.0	29.845967649087797	0.0
36	4.6801273369045826E-5	0.0	0.0	30.35605472753703	0.0
37	4.6801273369045826E-5	0.0	0.0	31.013519015825384	0.0
38	4.6801273369045826E-5	0.0	0.0	31.69344791533088	0.0
39	4.6801273369045826E-5	0.0	0.0	32.44240869305572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	25305	0.0	44.377594	1
CTAGCGG	565	0.0	42.610622	1
TAGTAGG	495	0.0	41.363636	1
CATGCGG	355	0.0	41.197186	1
CACTTAC	3420	0.0	41.18421	36
CCGGTAT	105	0.0	40.714287	42
TCGATTA	155	0.0	40.645164	40
ACTTACT	3445	0.0	40.624092	37
CGCACTT	3510	0.0	40.576923	34
CTTACGG	200	0.0	40.5	1
GCACTTA	3495	0.0	40.49356	35
CCAGTAG	1090	0.0	40.458714	25
CCGCACT	3530	0.0	40.347027	33
ACCCGCA	3565	0.0	40.26648	31
CCCGCAC	3595	0.0	40.243393	32
TGTACGG	235	0.0	40.212765	1
TACGGGT	140	0.0	40.17857	3
AATGCGG	280	0.0	40.17857	1
TAGCATA	1045	0.0	40.047848	29
AGGGCGA	815	0.0	40.030674	5
>>END_MODULE