##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043294_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2613965
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.411159675053035	33.0	31.0	34.0	31.0	34.0
2	32.73598919648886	34.0	31.0	34.0	31.0	34.0
3	32.835963373648845	34.0	31.0	34.0	31.0	34.0
4	36.26666539146469	37.0	37.0	37.0	35.0	37.0
5	36.19720730767244	37.0	37.0	37.0	35.0	37.0
6	36.35776951871965	37.0	37.0	37.0	35.0	37.0
7	36.29798562719853	37.0	37.0	37.0	35.0	37.0
8	36.28303056850417	37.0	37.0	37.0	35.0	37.0
9	37.91005350109891	39.0	38.0	39.0	35.0	39.0
10	37.708387832277786	39.0	37.0	39.0	35.0	39.0
11	37.33195356479524	39.0	37.0	39.0	35.0	39.0
12	37.3257415458891	39.0	37.0	39.0	35.0	39.0
13	37.3021781087352	39.0	37.0	39.0	35.0	39.0
14	38.622375203952615	40.0	38.0	41.0	35.0	41.0
15	38.682617020503336	41.0	38.0	41.0	35.0	41.0
16	38.5742050869082	41.0	38.0	41.0	34.0	41.0
17	38.53835609887661	40.0	38.0	41.0	34.0	41.0
18	38.302081320905216	40.0	37.0	41.0	34.0	41.0
19	38.0780236154654	40.0	37.0	41.0	34.0	41.0
20	37.782107641074	40.0	35.0	41.0	34.0	41.0
21	37.70902403054364	39.0	35.0	41.0	34.0	41.0
22	37.65388404205871	39.0	35.0	41.0	34.0	41.0
23	37.67762728269124	39.0	35.0	41.0	34.0	41.0
24	37.604825236757186	39.0	35.0	41.0	34.0	41.0
25	37.50266319556689	39.0	35.0	41.0	34.0	41.0
26	37.411819209515045	39.0	35.0	41.0	34.0	41.0
27	37.46079576428912	39.0	35.0	41.0	34.0	41.0
28	37.441243092390295	39.0	35.0	41.0	34.0	41.0
29	37.34309028621271	39.0	35.0	41.0	33.0	41.0
30	37.26559498692599	39.0	35.0	41.0	33.0	41.0
31	37.13249221010993	39.0	35.0	41.0	33.0	41.0
32	36.87750218537739	39.0	35.0	41.0	33.0	41.0
33	36.44759780639756	39.0	35.0	41.0	31.0	41.0
34	36.18576912850784	39.0	35.0	41.0	30.0	41.0
35	36.004792719106796	39.0	35.0	41.0	30.0	41.0
36	35.94473453164063	39.0	35.0	41.0	30.0	41.0
37	35.840291664195966	38.0	35.0	41.0	30.0	41.0
38	35.72970602131245	38.0	35.0	41.0	29.0	41.0
39	35.70503277587879	38.0	35.0	41.0	29.0	41.0
40	35.651808268282096	38.0	35.0	41.0	29.0	41.0
41	35.61376606037189	38.0	35.0	41.0	29.0	41.0
42	35.53078025145708	38.0	35.0	41.0	29.0	41.0
43	35.41895625993462	38.0	35.0	41.0	29.0	41.0
44	35.23915851972004	37.0	35.0	40.0	27.0	41.0
45	35.14884284984688	37.0	35.0	40.0	27.0	41.0
46	35.126518909013704	37.0	35.0	40.0	26.0	41.0
47	35.052644928298584	37.0	35.0	40.0	26.0	41.0
48	34.96590543484706	36.0	35.0	40.0	26.0	41.0
49	34.918518419336145	36.0	35.0	40.0	27.0	41.0
50	34.82042835309578	36.0	35.0	40.0	27.0	41.0
51	34.69567419609673	36.0	35.0	40.0	26.0	41.0
52	34.35090217351801	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	2.0
13	3.0
14	15.0
15	50.0
16	96.0
17	209.0
18	428.0
19	941.0
20	1661.0
21	2801.0
22	4546.0
23	7413.0
24	12935.0
25	23759.0
26	35898.0
27	44843.0
28	47588.0
29	46273.0
30	47570.0
31	52237.0
32	60932.0
33	79994.0
34	192861.0
35	251115.0
36	245486.0
37	236959.0
38	349735.0
39	864481.0
40	3128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.49980776330211	16.322674557616494	23.046789073304346	32.13072860577705
2	36.397962482282665	18.67358591258873	23.41358816969623	21.51486343543238
3	25.538367958254987	20.17861754078574	35.10242103471163	19.180593466247636
4	23.354520814165454	22.493529943974	32.93345549768264	21.218493744177906
5	21.422819356800876	30.24956340272345	30.115858475534296	18.211758764941383
6	76.22883244419876	1.9372485859604087	18.67262951110669	3.161289458734146
7	75.72136581782847	1.74761330010157	17.396598653769274	5.134422228300685
8	66.43952769069212	3.1854672882001096	22.84567696966103	7.529328051446749
9	41.83755329547259	13.823138412335284	26.808430870344473	17.530877421847652
10	29.17957968067667	14.579422448273025	36.51449043885439	19.726507432195916
11	26.092430464830247	11.941246344155335	40.18588619204924	21.780436998965175
12	21.080924955001308	12.66080456318275	43.1717716189773	23.08649886283864
13	16.543182483315576	15.378247222131897	45.60198778484027	22.476582509712255
14	17.187070217084006	15.853310966290673	44.87416625700803	22.085452559617284
15	20.96753399529068	13.77348204738778	43.81355526948524	21.445428687836294
16	20.100957740444116	18.042246166264658	40.42253817476515	21.43425791852607
17	20.385200260906323	19.307450558825387	40.2136218350284	20.093727345239895
18	20.1107512916202	25.166136501445123	36.38323389945925	18.339878307475423
19	21.842832631653447	21.682386719026457	34.38324537627703	22.091535273043057
20	20.953226229119366	25.715378744550904	34.53967440267945	18.791720623650278
21	19.034761368266217	19.971231443420244	36.56758984913723	24.42641733917631
22	20.271885813314256	21.75078855302194	34.049384746926606	23.927940886737197
23	21.394203824458245	19.29429047443252	36.69012400701616	22.621381694093074
24	23.287687478600517	17.663511179376922	34.20305933706075	24.84574200496181
25	18.499673867094625	18.040027314826325	38.07828337410792	25.382015443971135
26	17.653985420615808	17.90796739818628	37.70647273395015	26.731574447247763
27	21.216351404858138	20.755557170811393	36.45278341523318	21.57530800909729
28	22.74770320184088	21.852932231303786	32.82603248321993	22.573332083635396
29	22.00633902902296	19.424361075989925	36.782741926536886	21.78655796845023
30	25.930760358306248	20.10271751917107	31.81626379848238	22.150258324040298
31	26.450889740298745	18.279510245929075	33.82237329114965	21.447226722622528
32	23.710149141247108	22.21915748680644	32.31848934473109	21.75220402721536
33	20.998177098775233	21.827185903407276	37.493348227692415	19.68128877012508
34	21.488275474231674	25.204430816785994	34.18691528004392	19.120378428938416
35	22.823182406803458	28.25121989009034	31.553482927277145	17.372114775829058
36	26.567762001404	24.85392880164807	28.284349637428196	20.293959559519735
37	25.940974726134435	27.250288355046838	28.760369783069017	18.048367135749714
38	24.086282716103696	23.45306842287483	27.854619323518104	24.60602953750337
39	23.603338223732912	20.852612793208785	32.01075760386998	23.53329137918832
40	19.87627990428334	19.9436870807375	34.39847128787111	25.781561727108055
41	20.580344419301714	22.42199111311743	30.903588992201502	26.09407547537936
42	20.052487313334343	18.843213279443297	33.2921825655661	27.81211684165626
43	22.550569728362852	19.422869089677942	34.04490878799066	23.98165239396855
44	22.00565041995589	20.59832476716406	34.51163271122605	22.884392101654
45	22.642384270638665	21.535445195325874	33.26479122712049	22.557379306914974
46	23.066337919597242	19.86357889260185	32.947112910846165	24.122970276954742
47	20.70880826636929	20.59193600526403	34.37574718865785	24.323508539708833
48	22.23908889369215	18.659698963069513	31.337910033225384	27.76330211001295
49	21.571138098635597	17.17023755100011	35.91008295826455	25.348541392099744
50	20.945345480907356	17.149655791106614	36.96893416706038	24.936064560925644
51	19.105726358233564	16.206949978289686	36.538630012261066	28.148693651215684
52	17.028919668013916	17.06002184420985	40.37066295837932	25.540395529396914
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	498.0
1	3660.0
2	6822.0
3	92129.5
4	177437.0
5	100918.5
6	24400.0
7	22120.0
8	19840.0
9	19269.5
10	18699.0
11	18024.5
12	17350.0
13	16475.5
14	14489.0
15	13377.0
16	12428.5
17	11480.0
18	10871.5
19	10263.0
20	9760.0
21	9257.0
22	8981.0
23	8705.0
24	8439.0
25	8173.0
26	9006.5
27	9840.0
28	9988.5
29	10137.0
30	11163.0
31	12189.0
32	13317.5
33	14446.0
34	17346.0
35	20246.0
36	22902.5
37	25559.0
38	27875.0
39	35784.5
40	41378.0
41	52841.0
42	64304.0
43	91154.0
44	118004.0
45	160452.0
46	202900.0
47	228071.0
48	253242.0
49	248643.5
50	244045.0
51	221122.0
52	198199.0
53	171223.5
54	144248.0
55	135450.5
56	126653.0
57	124935.5
58	123218.0
59	122393.5
60	121569.0
61	121433.0
62	121297.0
63	120590.0
64	104529.0
65	89175.0
66	73866.0
67	58557.0
68	47782.5
69	37008.0
70	32321.5
71	27635.0
72	26398.5
73	25162.0
74	20334.5
75	15507.0
76	11978.0
77	8449.0
78	6426.5
79	4404.0
80	3394.0
81	2384.0
82	1839.0
83	1294.0
84	924.5
85	555.0
86	380.5
87	206.0
88	155.5
89	70.0
90	35.0
91	26.5
92	18.0
93	13.5
94	9.0
95	9.5
96	10.0
97	6.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2613965.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.28120790036106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6447140918347	21.564061840856617
2	10.224151914999581	5.9875103565595476
3	3.785341615230167	3.325181244283292
4	1.9517815217235759	2.286020820546844
5	1.290064350731572	1.8887321229307739
6	0.9593302677567153	1.6854209409176055
7	0.7456572573479986	1.5283621622373849
8	0.651309622774018	1.5256905977561408
9	0.5556721654677468	1.4643676981354437
>10	5.753780512297785	33.16541974280883
>50	0.2547352912887146	5.13896066422356
>100	0.17075230963947027	9.195590575259644
>500	0.006813114466178258	1.3325580278534415
>1k	0.004978814417591804	2.8647273708137364
>5k	5.240857281675582E-4	1.1476787413841107
>10k+	3.930642961256687E-4	5.899717093433012
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	129070	4.937709571474752	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13690	0.5237254515649598	No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	11020	0.42158177328311586	TruSeq Adapter, Index 15 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9390	0.35922439665412503	No Hit
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCTT	8880	0.3397138064205144	TruSeq Adapter, Index 21 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	6492	0.2483583368560788	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	5153	0.1971334734780305	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	4993	0.1910125039929762	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	4472	0.17108109710726807	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	4213	0.1611727777533364	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCG	3773	0.14434011166943705	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	3565	0.13638285133886643	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3389	0.1296497849053067	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3210	0.12280195029390217	No Hit
CGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	2870	0.10979489013816177	TruSeq Adapter, Index 21 (95% over 21bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2614	0.10000133896207486	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34801537128461935	0.0
2	3.8256059281589465E-5	0.0	0.0	1.1029221890882241	0.0
3	3.8256059281589465E-5	0.0	0.0	1.7790597808310364	0.0
4	3.8256059281589465E-5	0.0	0.0	2.265141270062912	0.0
5	3.8256059281589465E-5	0.0	0.0	4.650674358684986	0.0
6	3.8256059281589465E-5	0.0	0.0	5.7735662107182	0.0
7	3.8256059281589465E-5	0.0	0.0	8.764960510182807	0.0
8	3.8256059281589465E-5	0.0	0.0	12.29947608326814	0.0
9	3.8256059281589465E-5	0.0	0.0	15.73096808870815	0.0
10	3.8256059281589465E-5	0.0	0.0	17.57491014608076	0.0
11	3.8256059281589465E-5	0.0	0.0	18.93762923375026	0.0
12	3.8256059281589465E-5	0.0	0.0	19.8680548515378	0.0
13	7.651211856317893E-5	0.0	0.0	20.388796330478794	0.0
14	7.651211856317893E-5	0.0	0.0	20.699282507608174	0.0
15	7.651211856317893E-5	0.0	0.0	21.3733160160905	0.0
16	7.651211856317893E-5	0.0	0.0	22.081282649155593	0.0
17	7.651211856317893E-5	0.0	0.0	22.99215942065024	0.0
18	7.651211856317893E-5	0.0	0.0	23.536543144227256	0.0
19	7.651211856317893E-5	0.0	0.0	24.155028854632715	0.0
20	7.651211856317893E-5	0.0	0.0	24.84497688377618	0.0
21	7.651211856317893E-5	0.0	0.0	25.536646435587315	0.0
22	1.147681778447684E-4	0.0	0.0	26.185469201003073	0.0
23	1.5302423712635786E-4	0.0	0.0	26.765431059711972	0.0
24	1.912802964079473E-4	0.0	0.0	27.242025046242013	0.0
25	2.295363556895368E-4	0.0	0.0	27.70021786825761	0.0
26	2.295363556895368E-4	0.0	0.0	28.151256807187547	0.0
27	2.295363556895368E-4	0.0	0.0	28.63913633120566	0.0
28	2.295363556895368E-4	0.0	0.0	29.10394745147697	0.0
29	2.295363556895368E-4	0.0	0.0	29.598177481335824	0.0
30	2.295363556895368E-4	0.0	0.0	30.056982400299926	0.0
31	2.295363556895368E-4	0.0	0.0	30.534035459541347	0.0
32	2.6779241497112624E-4	0.0	0.0	31.062772454872196	0.0
33	2.6779241497112624E-4	0.0	0.0	31.572649212977222	0.0
34	3.060484742527157E-4	0.0	0.0	32.108807883808694	0.0
35	3.060484742527157E-4	0.0	0.0	32.61386437844424	0.0
36	3.4430453353430514E-4	0.0	0.0	33.13605958763794	0.0
37	3.4430453353430514E-4	0.0	0.0	33.82003967153347	0.0
38	3.4430453353430514E-4	0.0	0.0	34.503101610006254	0.0
39	3.4430453353430514E-4	0.0	0.0	35.25862052475836	0.0
40	3.4430453353430514E-4	0.0	0.0	35.90912655678251	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33050	0.0	45.297127	1
TCACGAC	445	0.0	42.898876	24
CTAGCGG	515	0.0	41.980587	1
GCATAGG	450	0.0	41.4	1
ACCCGCA	3715	0.0	41.23284	31
CCGCACT	3695	0.0	41.082542	33
GTTTTTT	37125	0.0	41.04377	2
CACTTAC	3700	0.0	40.902702	36
CCCGCAC	3760	0.0	40.800533	32
CGCACTT	3725	0.0	40.75168	34
CCGGTAT	170	0.0	40.588234	42
TACCGGT	170	0.0	40.588234	40
GCACTTA	3735	0.0	40.519413	35
TAGGGTA	755	0.0	40.516556	4
AACCCGA	125	0.0	40.48	23
CACGACG	455	0.0	40.439556	25
ACGTAGG	165	0.0	40.42424	1
TACGCGG	245	0.0	40.367348	1
ATTGCGG	570	0.0	40.35088	1
ACTTACT	3715	0.0	40.24226	37
>>END_MODULE