##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043293_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2590715
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3820427951357	33.0	31.0	34.0	31.0	34.0
2	32.66944607955719	34.0	31.0	34.0	31.0	34.0
3	32.74976753521711	34.0	31.0	34.0	31.0	34.0
4	36.23675742024885	37.0	37.0	37.0	35.0	37.0
5	36.212645929791584	37.0	37.0	37.0	35.0	37.0
6	36.373739295908656	37.0	37.0	37.0	35.0	37.0
7	36.32676384704609	37.0	37.0	37.0	35.0	37.0
8	36.23725187834246	37.0	37.0	37.0	35.0	37.0
9	37.87033000542321	39.0	38.0	39.0	35.0	39.0
10	37.65756325956348	39.0	37.0	39.0	35.0	39.0
11	37.32789558094966	39.0	37.0	39.0	35.0	39.0
12	37.32741733459682	39.0	37.0	39.0	35.0	39.0
13	37.317108597433524	39.0	37.0	39.0	35.0	39.0
14	38.63554848757968	40.0	38.0	41.0	35.0	41.0
15	38.702630354940624	40.0	38.0	41.0	35.0	41.0
16	38.634530621855355	41.0	38.0	41.0	34.0	41.0
17	38.5723165226588	40.0	38.0	41.0	34.0	41.0
18	38.35890439511872	40.0	37.0	41.0	34.0	41.0
19	38.155949226371874	40.0	37.0	41.0	34.0	41.0
20	37.87656148978178	40.0	35.0	41.0	34.0	41.0
21	37.80619404295725	40.0	35.0	41.0	34.0	41.0
22	37.76527676722449	40.0	35.0	41.0	34.0	41.0
23	37.75352132519401	39.0	35.0	41.0	34.0	41.0
24	37.68741795218695	39.0	35.0	41.0	34.0	41.0
25	37.6491929834042	39.0	35.0	41.0	34.0	41.0
26	37.626174241473876	39.0	35.0	41.0	34.0	41.0
27	37.61753724357948	39.0	35.0	41.0	34.0	41.0
28	37.534095027820506	39.0	35.0	41.0	34.0	41.0
29	37.47157213356159	39.0	35.0	41.0	34.0	41.0
30	37.35742835472061	39.0	35.0	41.0	34.0	41.0
31	37.22002458780684	39.0	35.0	41.0	33.0	41.0
32	36.97268939269661	39.0	35.0	41.0	33.0	41.0
33	36.54040602690763	39.0	35.0	41.0	31.0	41.0
34	36.232479450653585	39.0	35.0	41.0	31.0	41.0
35	36.05296028316507	39.0	35.0	41.0	30.0	41.0
36	35.97435804401488	39.0	35.0	41.0	30.0	41.0
37	35.81943324526241	38.0	35.0	41.0	30.0	41.0
38	35.707906118581164	38.0	35.0	41.0	29.0	41.0
39	35.641619784499646	38.0	35.0	41.0	29.0	41.0
40	35.56801886737831	38.0	35.0	41.0	29.0	41.0
41	35.49380267609521	38.0	35.0	40.0	29.0	41.0
42	35.401063027002195	38.0	35.0	40.0	29.0	41.0
43	35.28289217455413	37.0	35.0	40.0	28.0	41.0
44	35.165587492256	37.0	35.0	40.0	27.0	41.0
45	35.10541105447724	37.0	35.0	40.0	27.0	41.0
46	35.0304170856308	37.0	35.0	40.0	26.0	41.0
47	34.89978519443474	36.0	35.0	40.0	26.0	41.0
48	34.84157809716623	36.0	35.0	40.0	26.0	41.0
49	34.77414034349591	36.0	35.0	40.0	26.0	41.0
50	34.70466261244483	36.0	35.0	40.0	26.0	41.0
51	34.291136616725495	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	0.0
11	0.0
12	1.0
13	4.0
14	19.0
15	38.0
16	83.0
17	143.0
18	428.0
19	826.0
20	1540.0
21	2663.0
22	4057.0
23	6422.0
24	11122.0
25	19974.0
26	33106.0
27	43651.0
28	47157.0
29	46754.0
30	47277.0
31	52205.0
32	59756.0
33	78725.0
34	188363.0
35	254145.0
36	237030.0
37	236802.0
38	367193.0
39	850928.0
40	301.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.829068423195913	16.337072970203206	23.195334106607636	31.638524499993242
2	36.113196549987165	18.759879029534318	23.453872772574368	21.673051647904153
3	25.61150107209786	20.275792590076485	34.76179355892099	19.350912778904668
4	23.479695759664803	22.68427827839033	32.48994968570453	21.346076276240343
5	21.6333328830072	30.26554445394418	29.8140474733809	18.287075189667718
6	76.56341975091819	1.9229440521246064	18.341307322495915	3.172328874461297
7	76.06487012272673	1.7197569010871514	17.033251438309502	5.1821215378766095
8	66.66287106069174	3.130757339190146	22.56512198369948	7.6412496164186345
9	41.99099476399372	13.72902847283472	26.474004280671554	17.805972482500003
10	29.42635527257919	14.5944652345009	36.245283637914625	19.73389585500528
11	26.383488728015237	11.913467903648222	39.85864906020153	21.844394308135012
12	21.22016508956022	12.621071789062094	43.00403556547131	23.154727555906383
13	16.515903910696466	15.390654703431291	45.50342280026942	22.590018585602817
14	17.25087475851261	15.815132116037464	44.72641722458858	22.207575900861347
15	21.023771429894836	13.76546629019402	43.6647026014054	21.546059678505742
16	20.20612070412994	18.02046925269665	40.32346282782938	21.449947215344025
17	20.455125322546092	19.275991376897885	40.08985936314878	20.17902393740724
18	20.159801444774896	25.357439934535446	36.21212676809298	18.27063185259668
19	21.87376843844267	21.804945738917635	34.156902631126925	22.16438319151277
20	20.903418554337318	25.971015723458578	34.22977826584553	18.895787456358573
21	19.07662556475722	20.07438101064764	36.26825027067817	24.58074315391697
22	20.277568161685096	21.90704110641271	33.68220742150333	24.133183310398866
23	21.521047278454017	19.333427258498137	36.33819235230429	22.807333110743556
24	23.348149063096482	17.691795508189827	33.986138961638005	24.97391646707569
25	18.429584110950064	18.107935454112088	37.96245438035446	25.500026054583387
26	17.647599214888555	17.8060110818828	37.53990693688808	27.006482766340568
27	21.273779632263683	20.793371713986293	36.281644256508336	21.65120439724169
28	22.863997004687896	21.896889468737395	32.543873023470354	22.695240503104355
29	22.040440573355234	19.57046606824757	36.44434065499293	21.944752703404273
30	26.03331512729111	20.1560573046437	31.460812941601063	22.349814626464124
31	26.64372576682499	18.27063185259668	33.52526233105533	21.56038004952301
32	23.787294241165082	22.287360825100407	31.998618142095907	21.9267267916386
33	21.10652850660918	21.782558096896032	37.363391959362566	19.74752143713222
34	21.520043694501325	25.333045124608454	34.037823535201674	19.109087645688547
35	22.80930167926615	28.363173872849774	31.411367132239555	17.416157315644522
36	26.691511802726275	24.985534881297248	28.064646246306523	20.258307069669957
37	26.194004357870316	27.222291915552272	28.56558903623131	18.018114690346103
38	24.346174704666474	23.393619136030015	27.63202436393042	24.62818179537309
39	23.698129666906624	20.948734229739667	31.748301144664698	23.60483495868901
40	20.01709952657857	19.865982942932742	34.184694186739954	25.932223343748735
41	20.68892178414067	22.349390033253368	30.773859725982984	26.18782845662298
42	20.159299652798552	18.73123828749978	33.189177505051696	27.92028455464997
43	22.788110618111215	19.244610078684843	33.89855696207418	24.068722341129764
44	22.09378492037912	20.45917825773966	34.530699054122124	22.916337767759092
45	22.752406189025038	21.453189563498878	33.252248896540145	22.54215535093594
46	23.23628805175405	19.80893305516045	32.974989529917416	23.979789363168084
47	20.925188606234187	20.411739616283537	34.289182715968366	24.373889061513907
48	22.3584222888276	18.54812281551618	31.164717076173954	27.92873781948227
49	21.712886210949485	17.057491850705308	35.7661880986523	25.463433839692907
50	21.181256911701983	17.073008802589246	36.81856167119888	24.927172614509892
51	19.204235124280363	16.176538137155188	36.33958193008493	28.279644808479514
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	469.0
1	3388.5
2	6308.0
3	88218.5
4	170129.0
5	97714.0
6	25299.0
7	22482.5
8	19666.0
9	18951.5
10	18237.0
11	17503.0
12	16769.0
13	15923.0
14	15077.0
15	13934.5
16	12792.0
17	11823.0
18	10854.0
19	10262.5
20	9671.0
21	9241.0
22	8811.0
23	8491.0
24	8171.0
25	8005.0
26	8565.0
27	9291.0
28	9766.5
29	10242.0
30	11612.5
31	12983.0
32	14289.5
33	15596.0
34	19014.5
35	22433.0
36	24587.5
37	26742.0
38	30723.0
39	34704.0
40	42079.5
41	49455.0
42	61867.0
43	74279.0
44	109444.0
45	144609.0
46	188706.0
47	232803.0
48	261946.5
49	291090.0
50	261752.5
51	232415.0
52	197734.5
53	163054.0
54	145870.0
55	128686.0
56	126612.0
57	124538.0
58	124701.5
59	124865.0
60	123098.5
61	121332.0
62	119804.0
63	118276.0
64	114173.0
65	110070.0
66	90860.5
67	71651.0
68	58374.0
69	45097.0
70	38070.0
71	31043.0
72	28547.0
73	26051.0
74	22210.5
75	14332.0
76	10294.0
77	7692.5
78	5091.0
79	4006.0
80	2921.0
81	2195.0
82	1469.0
83	1054.0
84	639.0
85	480.0
86	321.0
87	234.0
88	147.0
89	89.5
90	32.0
91	24.0
92	16.0
93	13.5
94	11.0
95	9.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2590715.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.768565622347758
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.6615118291807	20.903674712767824
2	10.571180818120594	6.082354181436123
3	3.9364826831288577	3.3974088117248433
4	2.079460593375736	2.392923941584643
5	1.2934537240321469	1.8605404169644453
6	1.002468452913933	1.7303747683192747
7	0.8118806823437676	1.6349649881266206
8	0.7024664880321502	1.6167162606762462
9	0.5981001698957957	1.5485835587746086
>10	5.8873443268454	32.98391180401719
>50	0.2633243514136286	5.209410452308897
>100	0.17935284795157475	9.560932445678635
>500	0.006621652140848958	1.2704490392527752
>1k	0.005270294561083864	2.7649051584092956
>5k	6.756787898825467E-4	1.292414329107967
>10k+	4.0540727392952803E-4	5.750435130850829
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123925	4.783428512978078	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13066	0.5043395356108256	No Hit
CCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	10924	0.4216596576620739	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGCT	8739	0.3373200062531	TruSeq Adapter, Index 21 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7989	0.3083704691561982	No Hit
CGCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTG	6386	0.24649565853442004	TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCTGC	5129	0.19797623436001258	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	5001	0.19303551336214134	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTT	4355	0.16810031207600992	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTC	4308	0.16628614108460407	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCC	3566	0.13764539905006917	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCT	3398	0.13116070274036318	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3248	0.1253707953209828	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3184	0.12290043482204718	No Hit
CGTCTGTCTCTTATACACATCTGACGCTATAGGACTCGTATGCCGTCTTCT	2852	0.11008543973381865	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3460820661477623	0.0
2	0.0	0.0	0.0	1.1095006590844612	0.0
3	0.0	0.0	0.0	1.7973416605068484	0.0
4	0.0	0.0	0.0	2.2934981269649497	0.0
5	0.0	0.0	0.0	4.730547358547737	0.0
6	0.0	0.0	0.0	5.86320764730972	0.0
7	0.0	0.0	0.0	8.914450257940375	0.0
8	0.0	0.0	0.0	12.560509357455373	0.0
9	0.0	0.0	0.0	16.157971834030374	0.0
10	0.0	0.0	0.0	18.076515556516252	0.0
11	0.0	0.0	0.0	19.471072657548206	0.0
12	0.0	0.0	0.0	20.42999712434598	0.0
13	0.0	0.0	0.0	20.954369739627865	0.0
14	0.0	0.0	0.0	21.280264328573384	0.0
15	0.0	0.0	0.0	21.973239047907622	0.0
16	0.0	0.0	0.0	22.7069747154743	0.0
17	0.0	0.0	0.0	23.65053662791932	0.0
18	0.0	0.0	0.0	24.2188353408229	0.0
19	0.0	0.0	0.0	24.857384930414963	0.0
20	0.0	0.0	0.0	25.575140453504147	0.0
21	0.0	0.0	0.0	26.31246586367084	0.0
22	7.719876559173819E-5	0.0	0.0	26.987260273708223	0.0
23	7.719876559173819E-5	0.0	0.0	27.583968132349565	0.0
24	7.719876559173819E-5	0.0	0.0	28.058547543824773	0.0
25	7.719876559173819E-5	0.0	0.0	28.533281352831168	0.0
26	7.719876559173819E-5	0.0	0.0	28.99037524389985	0.0
27	7.719876559173819E-5	0.0	0.0	29.483019166523526	0.0
28	1.5439753118347637E-4	0.0	0.0	29.954240431695496	0.0
29	1.5439753118347637E-4	0.0	0.0	30.451825075317046	0.0
30	1.5439753118347637E-4	0.0	0.0	30.915944054054577	0.0
31	1.5439753118347637E-4	0.0	0.0	31.39442200319217	0.0
32	1.5439753118347637E-4	0.0	0.0	31.921843969714924	0.0
33	1.5439753118347637E-4	0.0	0.0	32.441623258444096	0.0
34	1.5439753118347637E-4	0.0	0.0	32.97969865461851	0.0
35	1.5439753118347637E-4	0.0	0.0	33.49137207296055	0.0
36	1.5439753118347637E-4	0.0	0.0	34.01532009503168	0.0
37	1.5439753118347637E-4	0.0	0.0	34.70404888225837	0.0
38	1.5439753118347637E-4	0.0	0.0	35.4064804503776	0.0
39	1.5439753118347637E-4	0.0	0.0	36.17491696307776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	31835	0.0	44.300297	1
ACCGGTA	155	0.0	43.548386	41
TCACGAC	350	0.0	43.071426	24
TCGACGG	185	0.0	41.351353	1
CCGGTAT	160	0.0	40.781254	42
CACTTAC	3295	0.0	40.56146	36
CGTAAGG	100	0.0	40.5	1
CATGCGG	395	0.0	40.44304	1
CGCACTT	3320	0.0	40.256023	34
GCACTTA	3340	0.0	40.01497	35
CGACGGT	360	0.0	40.0	27
ACCCGCA	3430	0.0	39.94898	31
GTTTTTT	35980	0.0	39.934685	2
ACACGAC	215	0.0	39.767445	25
CCGCACT	3365	0.0	39.650818	33
CCCGCAC	3470	0.0	39.55331	32
CTAGCGG	660	0.0	39.545452	1
ACTTACT	3335	0.0	39.467766	37
GGCGATA	475	0.0	39.315792	7
TACGGGA	350	0.0	39.214287	3
>>END_MODULE