FastQCFastQC Report
Thu 26 May 2016
SRR1043292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043292_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2806781
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1077053.83731399065335No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC214950.7658239100236177No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA148030.5274013184498542No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT78580.2799648422873035No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT68340.2434817679042291Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC56560.2015119811627626No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54680.19481391672524503No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT49940.17792624362214224No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC44790.15957782242362337No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC35230.12551745219879998No Hit
GGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT29480.10503135086064783No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT28270.10072036257905409No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA28170.10036408255578189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT274550.045.2292861
TCACGAC6100.043.36065324
CACGACG6000.043.31666625
GCGATCG700.042.7142838
CACTTAC54950.042.27479636
ACTTACT54600.042.20879437
CTTACTG54700.042.04753538
GCACTTA55200.042.035
CGACGGT6100.041.8524627
AATTCGG554.7293724E-1141.8181841
CGCACTT55600.041.6978434
CCCGCAC56300.041.62877332
CCGCACT55650.041.57771733
TATTAGG3050.041.4754071
ACCCGCA56450.041.4366731
AATGCGG3500.041.41
GACGGTC6400.041.32812528
AGGGCGA13250.041.139625
TAGTAGG5100.041.0392151
GTTGATC23200.040.8448315