##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043292_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2806781
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.337272127750616	33.0	31.0	34.0	31.0	34.0
2	32.68203433043048	34.0	31.0	34.0	31.0	34.0
3	32.83080190438798	34.0	31.0	34.0	31.0	34.0
4	36.240104945843655	37.0	37.0	37.0	35.0	37.0
5	36.187995073359836	37.0	37.0	37.0	35.0	37.0
6	36.31756556710338	37.0	37.0	37.0	35.0	37.0
7	36.268113187313155	37.0	37.0	37.0	35.0	37.0
8	36.260431433731384	37.0	37.0	37.0	35.0	37.0
9	37.826616326674575	39.0	38.0	39.0	35.0	39.0
10	37.62548307117655	39.0	37.0	39.0	35.0	39.0
11	37.2642008763776	39.0	37.0	39.0	35.0	39.0
12	37.26543146757798	39.0	37.0	39.0	35.0	39.0
13	37.25545562692636	39.0	37.0	39.0	35.0	39.0
14	38.50901798180905	40.0	37.0	41.0	35.0	41.0
15	38.5758511262546	40.0	37.0	41.0	35.0	41.0
16	38.42850867239019	40.0	37.0	41.0	34.0	41.0
17	38.3847122379694	40.0	37.0	41.0	34.0	41.0
18	38.250868521626735	40.0	37.0	41.0	34.0	41.0
19	38.068075136606666	40.0	37.0	41.0	34.0	41.0
20	37.76216740814478	40.0	35.0	41.0	34.0	41.0
21	37.7426959923129	40.0	35.0	41.0	34.0	41.0
22	37.70074686981278	39.0	35.0	41.0	34.0	41.0
23	37.71641749035639	39.0	35.0	41.0	34.0	41.0
24	37.64759202802071	39.0	35.0	41.0	34.0	41.0
25	37.54325827344563	39.0	35.0	41.0	34.0	41.0
26	37.439395521061314	39.0	35.0	41.0	34.0	41.0
27	37.498415444596496	39.0	35.0	41.0	34.0	41.0
28	37.45986558979842	39.0	35.0	41.0	34.0	41.0
29	37.358577316862274	39.0	35.0	41.0	33.0	41.0
30	37.30346614146241	39.0	35.0	41.0	33.0	41.0
31	37.21004239375997	39.0	35.0	41.0	33.0	41.0
32	36.98546021225026	39.0	35.0	41.0	33.0	41.0
33	36.57158111017568	39.0	35.0	41.0	31.0	41.0
34	36.375000400815026	39.0	35.0	41.0	31.0	41.0
35	36.262876583531096	39.0	35.0	41.0	31.0	41.0
36	36.24151581473581	39.0	35.0	41.0	31.0	41.0
37	36.15147815237455	39.0	35.0	41.0	31.0	41.0
38	36.045391500084975	38.0	35.0	41.0	31.0	41.0
39	35.98361860080997	38.0	35.0	41.0	30.0	41.0
40	35.89292573948591	38.0	35.0	41.0	30.0	41.0
41	35.8219672286509	38.0	35.0	41.0	30.0	41.0
42	35.74588719248135	38.0	35.0	41.0	30.0	41.0
43	35.65405280996273	37.0	35.0	40.0	30.0	41.0
44	35.45755155104727	37.0	35.0	40.0	30.0	41.0
45	35.37049523992075	37.0	35.0	40.0	29.0	41.0
46	35.35382667903196	37.0	35.0	40.0	29.0	41.0
47	35.27466232670094	37.0	35.0	40.0	29.0	41.0
48	35.18006748656201	36.0	35.0	40.0	29.0	41.0
49	35.121076421708715	36.0	35.0	40.0	29.0	41.0
50	35.019543740676596	36.0	35.0	40.0	28.0	41.0
51	34.90606748442433	36.0	35.0	40.0	28.0	41.0
52	34.52902951815621	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	1.0
11	3.0
12	6.0
13	9.0
14	21.0
15	55.0
16	109.0
17	254.0
18	533.0
19	1028.0
20	1869.0
21	3113.0
22	4796.0
23	7668.0
24	13476.0
25	23562.0
26	35255.0
27	42599.0
28	44061.0
29	44323.0
30	47476.0
31	54211.0
32	65348.0
33	86478.0
34	210389.0
35	292863.0
36	249382.0
37	265019.0
38	389496.0
39	920553.0
40	2824.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.61089589818372	17.4453582235308	24.226542790477776	28.717203087807707
2	33.89904663028573	20.13530802723832	24.437318052245615	21.52832729023034
3	26.90006808511245	20.887700180384574	32.64871751661423	19.56351421788875
4	23.52427923660592	23.732631794215507	30.418226430918548	22.32486253826002
5	21.895188830193735	32.09904869670986	27.236004519055818	18.76975795404059
6	80.3878535589346	1.9029272322992068	15.184440823847675	2.524778384918524
7	80.23992609327198	1.7621253671020292	13.800684841460734	4.197263698165265
8	71.47999790507346	3.13305526865117	18.73562632781111	6.651320498464256
9	43.45971417078853	15.052474703227647	24.21670946183546	17.27110166414836
10	29.94273511185946	15.88567116565204	32.679820762645896	21.49177295984261
11	26.683093550939667	12.6251745326764	37.441717041692954	23.250014874690972
12	22.583058671125393	13.983207097383088	39.52862727800993	23.905106953481585
13	18.026486569490103	17.815960703738554	41.979940722129726	22.177612004641617
14	18.115841599326775	17.276053956471845	42.338643449560195	22.26946099464119
15	22.46587817147116	14.60587769405593	41.318150578901594	21.61009355557131
16	21.420801979206786	17.99830481964927	37.568267705959244	23.012625495184697
17	21.193495324359116	20.210875020174356	37.77711905560142	20.81851059986511
18	22.50745605018703	25.505160537997085	33.052774691007244	18.93460872080864
19	24.446296308832075	21.868040292420392	31.20760045048046	22.478062948267073
20	22.663542328382587	25.571571134335024	33.269856109187	18.495030428095387
21	20.55112244239932	20.068220498856164	33.903535758578954	25.47712130016556
22	21.887813833712	21.38431890482371	32.29510959351656	24.43275766794773
23	22.828642491166928	19.396775166997354	34.962043707720696	22.812538634115022
24	25.763178530850823	17.73700905058143	31.53587686392348	24.96393555464427
25	18.912198707344817	18.9896539844042	34.698432118501586	27.399715189749397
26	17.839938349304774	19.890222999229366	34.9616517996951	27.308186851770767
27	21.517460749520538	22.239462216681673	33.3808373364363	22.862239697361495
28	24.0008037677325	22.62413775780868	30.57449084912574	22.800567625333077
29	23.017720299517492	20.913245458053193	33.779051518447645	22.289982723981673
30	26.153412040340875	22.149252114789146	29.351559669243876	22.3457761756261
31	27.255314896317167	21.339748273912356	29.17007062538901	22.234866204381458
32	27.610846731540505	22.988540965611495	26.801485402673027	22.59912690017497
33	20.697339763950236	25.966578796136925	32.83409001272276	20.50199142719008
34	21.73229760355368	25.162597295620852	31.205284630329196	21.899820470496273
35	23.971018757786947	28.9910042856924	28.27242310675468	18.76555384976598
36	26.126691038595457	26.149564216089534	26.10093199291288	21.62281275240213
37	23.06033139030085	29.790888565940843	26.59851267341485	20.550267370343462
38	23.561938035065793	24.33549322159442	25.842058928003286	26.2605098153365
39	24.75854012122784	21.43010088781419	31.287834711721363	22.523524279236607
40	20.76186207616483	21.50919505298062	33.33819061765061	24.39075225320394
41	21.569940796948533	24.77047550200746	28.05124446830729	25.60833923273672
42	20.7565891318204	19.206272238553705	31.099398207412694	28.937740422213203
43	22.524664375311076	20.109228329534794	32.609206062033344	24.756901233120786
44	23.519148804270802	21.554656383950153	31.41082257575493	23.515372236024117
45	22.85454404885882	23.27082162805007	30.442453472501064	23.432180850590054
46	22.65666612393343	22.60732134071023	29.787076369691828	24.94893616566451
47	20.61215321038585	23.417003321598656	32.25884028714745	23.712003180868045
48	23.315392258961424	20.80133790274339	29.7116518887651	26.171617949530084
49	21.602754187091904	19.284725099678244	33.653569694251175	25.45895101897868
50	20.830517236649385	19.120871916975354	35.043560577045376	25.00505026932988
51	19.773149383582115	17.703875008417118	34.241752384671265	28.281223223329498
52	17.729812194111332	18.72230145494073	37.12605294107378	26.421833409874157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	713.0
1	4020.5
2	7328.0
3	77544.5
4	147761.0
5	84059.0
6	20357.0
7	18322.0
8	16287.0
9	15915.0
10	15543.0
11	15235.5
12	14928.0
13	14334.0
14	13039.0
15	12338.0
16	11604.5
17	10871.0
18	10260.0
19	9649.0
20	9111.0
21	8573.0
22	8193.5
23	7814.0
24	8397.0
25	8980.0
26	9649.0
27	10318.0
28	10905.0
29	11492.0
30	12634.0
31	13776.0
32	16331.0
33	18886.0
34	22093.0
35	25300.0
36	28975.0
37	32650.0
38	36752.0
39	45787.5
40	50721.0
41	63472.5
42	76224.0
43	102521.5
44	128819.0
45	168766.0
46	208713.0
47	229730.5
48	250748.0
49	247044.0
50	243340.0
51	223384.0
52	203428.0
53	177357.0
54	151286.0
55	147016.5
56	142747.0
57	143627.0
58	144507.0
59	144293.0
60	144079.0
61	144909.0
62	145739.0
63	147640.0
64	127124.0
65	104707.0
66	87791.5
67	70876.0
68	57008.0
69	43140.0
70	37424.0
71	31708.0
72	32015.5
73	32323.0
74	25045.0
75	17767.0
76	13393.0
77	9019.0
78	6961.0
79	4903.0
80	3469.5
81	2036.0
82	1672.5
83	1309.0
84	960.5
85	612.0
86	407.5
87	203.0
88	147.5
89	56.5
90	21.0
91	14.5
92	8.0
93	6.5
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2806781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.541489468257414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.66636437431276	19.552150342056354
2	10.464026603994862	5.5546170381099005
3	3.8672673699476365	3.079291085112022
4	1.916206735496628	2.0343592355675066
5	1.182644954304386	1.5694579299678817
6	0.8035156940497707	1.2795901998720922
7	0.6160923503383386	1.144640603958528
8	0.4736462585412695	1.0057021746202106
9	0.4361181270535467	1.0417702208497073
>10	5.758533334406138	34.91837081857337
>50	0.6314621281860008	10.97006791303867
>100	0.17058636537728	7.914673576274042
>500	0.007579994235258557	1.3763882724015868
>1k	0.00500821047686726	2.4580414908570827
>5k	5.414281596613253E-4	0.9274597943286531
>10k+	4.06071119745994E-4	5.173419304412342
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107705	3.83731399065335	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	21495	0.7658239100236177	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	14803	0.5274013184498542	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	7858	0.2799648422873035	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT	6834	0.2434817679042291	Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5656	0.2015119811627626	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5468	0.19481391672524503	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	4994	0.17792624362214224	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4479	0.15957782242362337	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3523	0.12551745219879998	No Hit
GGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	2948	0.10503135086064783	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	2827	0.10072036257905409	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	2817	0.10036408255578189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25235314048370716	0.0
2	0.0	0.0	0.0	0.8371511706827145	0.0
3	0.0	0.0	0.0	1.399574815420227	0.0
4	0.0	0.0	0.0	1.7820414204029456	0.0
5	3.562800232722111E-5	0.0	0.0	3.8263405659365657	0.0
6	3.562800232722111E-5	0.0	0.0	4.601107104544316	0.0
7	3.562800232722111E-5	0.0	0.0	6.8935552862870315	0.0
8	3.562800232722111E-5	0.0	0.0	9.595226702760208	0.0
9	3.562800232722111E-5	0.0	0.0	12.050779879156941	0.0
10	3.562800232722111E-5	0.0	0.0	13.459475463172938	0.0
11	3.562800232722111E-5	0.0	0.0	14.496606610918343	0.0
12	3.562800232722111E-5	0.0	0.0	15.210092985523273	0.0
13	3.562800232722111E-5	0.0	0.0	15.575992569423835	0.0
14	3.562800232722111E-5	0.0	0.0	15.811030500776512	0.0
15	3.562800232722111E-5	0.0	0.0	16.45375966275958	0.0
16	3.562800232722111E-5	0.0	0.0	17.136071535328192	0.0
17	3.562800232722111E-5	0.0	0.0	18.043481126600188	0.0
18	3.562800232722111E-5	0.0	0.0	18.556310592098207	0.0
19	7.125600465444222E-5	0.0	0.0	19.100029535613928	0.0
20	1.0688400698166334E-4	0.0	0.0	19.764099870991004	0.0
21	1.0688400698166334E-4	0.0	0.0	20.460271036464903	0.0
22	1.4251200930888444E-4	0.0	0.0	21.091813005717224	0.0
23	1.4251200930888444E-4	0.0	0.0	21.705042181773354	0.0
24	1.4251200930888444E-4	0.0	0.0	22.156983391294155	0.0
25	1.4251200930888444E-4	0.0	0.0	22.60472049654034	0.0
26	1.4251200930888444E-4	0.0	0.0	23.01326679922659	0.0
27	1.4251200930888444E-4	0.0	0.0	23.473188681268685	0.0
28	1.7814001163610557E-4	0.0	0.0	23.917505498291458	0.0
29	1.7814001163610557E-4	0.0	0.0	24.386655032936307	0.0
30	1.7814001163610557E-4	0.0	0.0	24.824808205556472	0.0
31	1.7814001163610557E-4	0.0	0.0	25.27436233892135	0.0
32	1.7814001163610557E-4	0.0	0.0	25.78630110436119	0.0
33	1.7814001163610557E-4	0.0	0.0	26.26578275968093	0.0
34	1.7814001163610557E-4	0.0	0.0	26.79079700197486	0.0
35	1.7814001163610557E-4	0.0	0.0	27.23450814295807	0.0
36	1.7814001163610557E-4	0.0	0.0	27.734725295632256	0.0
37	1.7814001163610557E-4	0.0	0.0	28.3698300651173	0.0
38	2.1376801396332667E-4	0.0	0.0	29.04359121712738	0.0
39	2.1376801396332667E-4	0.0	0.0	29.800935662597116	0.0
40	2.1376801396332667E-4	0.0	0.0	30.486632195386814	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27455	0.0	45.229286	1
TCACGAC	610	0.0	43.360653	24
CACGACG	600	0.0	43.316666	25
GCGATCG	70	0.0	42.714283	8
CACTTAC	5495	0.0	42.274796	36
ACTTACT	5460	0.0	42.208794	37
CTTACTG	5470	0.0	42.047535	38
GCACTTA	5520	0.0	42.0	35
CGACGGT	610	0.0	41.85246	27
AATTCGG	55	4.7293724E-11	41.818184	1
CGCACTT	5560	0.0	41.69784	34
CCCGCAC	5630	0.0	41.628773	32
CCGCACT	5565	0.0	41.577717	33
TATTAGG	305	0.0	41.475407	1
ACCCGCA	5645	0.0	41.43667	31
AATGCGG	350	0.0	41.4	1
GACGGTC	640	0.0	41.328125	28
AGGGCGA	1325	0.0	41.13962	5
TAGTAGG	510	0.0	41.039215	1
GTTGATC	2320	0.0	40.84483	15
>>END_MODULE