FastQCFastQC Report
Thu 26 May 2016
SRR1043291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043291_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2750598
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1025853.729552628192124No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC210700.766015244684974No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG121010.43994069653217227No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC74870.27219535533727574No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT65490.2380936799924962No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC55000.19995651854614888No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC50960.18526880336566812No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC48860.1776340999302697No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG44630.16225562586753864No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC34440.12520913634053396No Hit
GGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC29240.1063041564052617No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC28480.10354112087626036No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT260000.044.1865431
ATTGCGG5650.042.2123871
CATGCGG4050.041.6666681
CACTTAC51600.041.51162736
CGCACTT51700.041.43133534
GCACTTA51700.041.43133535
ACCCGCA52900.041.2996231
CCGCACT52050.041.23919333
CCCGCAC53050.041.14043432
TCACGAC5700.041.0526324
TAGTAGG5100.041.029411
ACTTACT52250.040.90909237
CTTACTG52550.040.7183638
CGACGGT5700.040.65789427
GCGCGAC23950.040.5845538
TAGGGTA11050.040.520364
AGGGTAC11700.040.3846175
TTATGAC59000.040.0042426
TATGCGG4450.039.9438171
TCAACGC61550.039.8822115