##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043291_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2750598
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.366351607904896	33.0	31.0	34.0	31.0	34.0
2	32.654502766307544	34.0	31.0	34.0	31.0	34.0
3	32.7711141359079	34.0	31.0	34.0	31.0	34.0
4	36.226916474163076	37.0	37.0	37.0	35.0	37.0
5	36.21779518490161	37.0	37.0	37.0	35.0	37.0
6	36.35361910391849	37.0	37.0	37.0	35.0	37.0
7	36.311265768389276	37.0	37.0	37.0	35.0	37.0
8	36.22802605106235	37.0	37.0	37.0	35.0	37.0
9	37.80750113248101	39.0	38.0	39.0	35.0	39.0
10	37.58527636535764	39.0	37.0	39.0	35.0	39.0
11	37.263794636657195	39.0	37.0	39.0	35.0	39.0
12	37.27104869559274	39.0	37.0	39.0	35.0	39.0
13	37.2735837806906	39.0	37.0	39.0	35.0	39.0
14	38.5322024519759	40.0	37.0	41.0	35.0	41.0
15	38.611179459884724	40.0	37.0	41.0	35.0	41.0
16	38.506085949309934	40.0	37.0	41.0	34.0	41.0
17	38.43879949014723	40.0	37.0	41.0	34.0	41.0
18	38.31872196518721	40.0	37.0	41.0	34.0	41.0
19	38.155251694358824	40.0	37.0	41.0	34.0	41.0
20	37.87208527018488	40.0	35.0	41.0	34.0	41.0
21	37.84991045583542	40.0	35.0	41.0	34.0	41.0
22	37.822551677853326	40.0	35.0	41.0	34.0	41.0
23	37.80716047928487	40.0	35.0	41.0	34.0	41.0
24	37.74194338831047	40.0	35.0	41.0	34.0	41.0
25	37.70582724193066	40.0	35.0	41.0	34.0	41.0
26	37.67221091558999	39.0	35.0	41.0	34.0	41.0
27	37.665817033241495	39.0	35.0	41.0	34.0	41.0
28	37.55999059113691	39.0	35.0	41.0	34.0	41.0
29	37.504110742464	39.0	35.0	41.0	34.0	41.0
30	37.40200058314592	39.0	35.0	41.0	34.0	41.0
31	37.29387245973421	39.0	35.0	41.0	33.0	41.0
32	37.08421077889245	39.0	35.0	41.0	33.0	41.0
33	36.667705349891186	39.0	35.0	41.0	32.0	41.0
34	36.43560454853817	39.0	35.0	41.0	31.0	41.0
35	36.31654207557775	39.0	35.0	41.0	31.0	41.0
36	36.268656488516314	39.0	35.0	41.0	31.0	41.0
37	36.13318921921706	39.0	35.0	41.0	31.0	41.0
38	36.031189581320135	38.0	35.0	41.0	31.0	41.0
39	35.93098991564744	38.0	35.0	41.0	30.0	41.0
40	35.81760111801143	38.0	35.0	41.0	30.0	41.0
41	35.70785043834105	38.0	35.0	40.0	30.0	41.0
42	35.61666117695134	38.0	35.0	40.0	30.0	41.0
43	35.515290856751875	37.0	35.0	40.0	30.0	41.0
44	35.40443823488565	37.0	35.0	40.0	29.0	41.0
45	35.339577066514266	37.0	35.0	40.0	29.0	41.0
46	35.25114538729396	37.0	35.0	40.0	29.0	41.0
47	35.11204545338868	36.0	35.0	40.0	29.0	41.0
48	35.042828868486055	36.0	35.0	40.0	28.0	41.0
49	34.966173173978895	36.0	35.0	40.0	28.0	41.0
50	34.892777134281346	36.0	35.0	40.0	28.0	41.0
51	34.487339116802964	35.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	11.0
14	23.0
15	46.0
16	107.0
17	217.0
18	478.0
19	944.0
20	1617.0
21	2727.0
22	4364.0
23	6729.0
24	11222.0
25	19842.0
26	32312.0
27	41225.0
28	43832.0
29	43762.0
30	46041.0
31	52690.0
32	61950.0
33	82600.0
34	203085.0
35	292511.0
36	237711.0
37	260026.0
38	404077.0
39	900162.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.9354540358133	17.486415681244587	24.258288561251042	28.319841721691063
2	33.609818664886696	20.166342009992007	24.51496001960301	21.70887930551829
3	26.926108431693763	21.02677308716141	32.386121127114905	19.660997354029924
4	23.73851068022299	23.809949690939934	30.031033251678362	22.420506377158713
5	22.06596529191107	32.211504552828146	26.918473728258363	18.80405642700242
6	80.68071015830012	1.8847174323547096	14.901196030826751	2.5333763785184167
7	80.5529924765451	1.755909078680345	13.468416686116983	4.222681758657572
8	71.76453992913541	3.075149476586546	18.513719562073412	6.6465910322046335
9	43.671267120822456	15.044546676759019	23.87760770566982	17.406578496748708
10	30.303555808591444	15.86207799176761	32.339367657505754	21.494998542135203
11	26.916292384419677	12.62303688143451	37.2383023618864	23.222368372259414
12	22.79228007873197	13.9149741256265	39.433497733947306	23.85924806169422
13	18.08555812227014	17.784641739723508	41.865768825542666	22.26403131246369
14	18.195861409046323	17.245595321453735	42.28280541176864	22.2757378577313
15	22.55982153698941	14.540438115638853	41.24935741246085	21.65038293491088
16	21.528227679944507	17.982707760276128	37.48203118012883	23.007033379650537
17	21.389966836302506	20.109590714455546	37.66322814166229	20.83721430757966
18	22.614682334532347	25.696884822863975	32.910807031779996	18.777625810823682
19	24.550952192941317	21.922251088672354	30.88684715105588	22.63994956733045
20	22.742618150671237	25.786029074404908	32.890738668464095	18.58061410645976
21	20.559892794221472	20.163106349964625	33.764112385743026	25.51288847007087
22	21.918651871338525	21.524483039688096	31.970647837306654	24.586217251666728
23	22.972895348575108	19.453187997664507	34.69372841832939	22.880188235430985
24	25.795772410217705	17.728653914530586	31.37052379155369	25.105049883698015
25	18.84150282956652	19.006194289387253	34.64421191319124	27.508090967854994
26	17.90243430701251	19.746215186661228	34.83875142787132	27.51259907845494
27	21.53353561661864	22.252615613041236	33.25135116072941	22.96249760961071
28	24.021867244868204	22.700772704699123	30.280797121207826	22.996562929224844
29	23.032336968179283	21.055239624256252	33.58422423051278	22.32819917705168
30	26.301444267755592	22.17884983556303	28.988423608248098	22.531282288433278
31	27.328675437123128	21.409380796466802	28.96068418576615	22.30125958064392
32	27.76210845786989	23.010269039677915	26.547536208489937	22.68008629396226
33	20.770465186115892	26.028739932189293	32.661770276863436	20.539024604831386
34	21.763994593175738	25.273485983775164	31.01889116475763	21.94362825829147
35	24.005398098886133	29.15675064113331	28.103416057162846	18.734435202817714
36	26.202883881977662	26.14664156667023	25.949375372191792	21.701099179160313
37	23.124426033902445	29.771380623413528	26.5328848490401	20.57130849364393
38	23.74490928881647	24.351213808779036	25.634534744808217	26.269342157596277
39	24.79024561204509	21.51204937980759	31.089748483784252	22.607956524363065
40	20.908616962565958	21.504923656601218	33.13334045905654	24.45311892177628
41	21.725639297345523	24.762288055179273	27.90349589434734	25.608576753127863
42	20.81318316962348	19.155543630875904	31.061027456574898	28.97024574292572
43	22.73138422990201	20.028408367925813	32.50624046116517	24.73396694100701
44	23.495399909401517	21.47165816306127	31.43429174310459	23.59865018443262
45	22.970641293275136	23.221750324838453	30.40237068448388	23.40523769740253
46	22.814929698923653	22.578326603887593	29.699214498083688	24.907529199105067
47	20.808565991831596	23.300460481684347	32.1314492339484	23.759524292535662
48	23.445665269879495	20.66416102971063	29.578186270767304	26.311987429642574
49	21.688665519279805	19.227418910360583	33.576153258309645	25.507762312049962
50	20.906362907265983	19.00317676374374	35.07655426201866	25.013906066971618
51	19.795586268876804	17.690189551508436	34.07808047559113	28.436143704023635
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	558.0
1	3873.0
2	7188.0
3	73765.0
4	140342.0
5	80612.5
6	20883.0
7	18453.5
8	16024.0
9	15729.0
10	15434.0
11	14953.0
12	14472.0
13	13846.0
14	13220.0
15	12248.0
16	11276.0
17	10664.5
18	10053.0
19	9472.0
20	8891.0
21	8320.5
22	7750.0
23	7605.5
24	7461.0
25	7864.5
26	9020.5
27	9773.0
28	10601.5
29	11430.0
30	13117.0
31	14804.0
32	17010.5
33	19217.0
34	23988.5
35	28760.0
36	32332.5
37	35905.0
38	39821.0
39	43737.0
40	51300.5
41	58864.0
42	72839.5
43	86815.0
44	117800.5
45	148786.0
46	191520.0
47	234254.0
48	258683.5
49	283113.0
50	257896.5
51	232680.0
52	198393.0
53	164106.0
54	152648.5
55	141191.0
56	142161.5
57	143132.0
58	143553.5
59	143975.0
60	145104.5
61	146234.0
62	144931.5
63	143629.0
64	137803.5
65	131978.0
66	108652.0
67	85326.0
68	67742.0
69	50158.0
70	42991.0
71	35824.0
72	33444.0
73	31064.0
74	26276.0
75	16352.5
76	11217.0
77	8471.0
78	5725.0
79	4298.0
80	2871.0
81	2182.5
82	1494.0
83	1134.0
84	774.0
85	530.0
86	286.0
87	195.0
88	104.0
89	69.0
90	34.0
91	23.0
92	12.0
93	11.0
94	10.0
95	8.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2750598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.287334717861555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.91036691657399	19.166192195380802
2	10.7491757971505	5.65134364241663
3	3.9782917969143585	3.1373606421243196
4	1.933605586814784	2.0331734909170933
5	1.2249954981367304	1.6100933343696893
6	0.8360189678834049	1.318602626353933
7	0.6683896352243421	1.2299127444164168
8	0.5462997211556617	1.148861090103465
9	0.45145297196385986	1.0680745845048618
>10	5.90048625677597	35.37714352544255
>50	0.6117424970272256	10.504571911583648
>100	0.17505505100603183	7.992898371436703
>500	0.008387704974177962	1.5159818013789432
>1k	0.004753032818700845	2.3517874419520575
>5k	5.591803316118641E-4	0.9051866223442204
>10k+	4.193852487088981E-4	4.9888159752745205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102585	3.729552628192124	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	21070	0.766015244684974	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	12101	0.43994069653217227	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	7487	0.27219535533727574	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	6549	0.2380936799924962	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5500	0.19995651854614888	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5096	0.18526880336566812	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	4886	0.1776340999302697	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	4463	0.16225562586753864	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3444	0.12520913634053396	No Hit
GGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	2924	0.1063041564052617	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	2848	0.10354112087626036	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2452557589295128	0.0
2	0.0	0.0	0.0	0.8230573860665935	0.0
3	0.0	0.0	0.0	1.389188823666708	0.0
4	0.0	0.0	0.0	1.769833323517286	0.0
5	0.0	0.0	0.0	3.8669409342986505	0.0
6	0.0	0.0	0.0	4.6562238465962675	0.0
7	0.0	0.0	0.0	7.02003709738755	0.0
8	0.0	0.0	0.0	9.796269756612926	0.0
9	0.0	0.0	0.0	12.347096885840825	0.0
10	0.0	0.0	0.0	13.814159684548596	0.0
11	0.0	0.0	0.0	14.88359985719469	0.0
12	0.0	0.0	0.0	15.604715774533393	0.0
13	0.0	0.0	0.0	15.98092487524531	0.0
14	0.0	0.0	0.0	16.230652389044128	0.0
15	0.0	0.0	0.0	16.88981814136417	0.0
16	0.0	0.0	0.0	17.589484177622467	0.0
17	0.0	0.0	0.0	18.51171999688795	0.0
18	0.0	0.0	0.0	19.040514099115903	0.0
19	0.0	0.0	0.0	19.62096969459005	0.0
20	3.635573064475434E-5	0.0	0.0	20.30453014217272	0.0
21	3.635573064475434E-5	0.0	0.0	21.026373174124316	0.0
22	3.635573064475434E-5	0.0	0.0	21.666924792354244	0.0
23	3.635573064475434E-5	0.0	0.0	22.286935422769886	0.0
24	3.635573064475434E-5	0.0	0.0	22.75432469593885	0.0
25	3.635573064475434E-5	0.0	0.0	23.213024949483714	0.0
26	7.271146128950868E-5	0.0	0.0	23.636169298458007	0.0
27	7.271146128950868E-5	0.0	0.0	24.10479466646889	0.0
28	7.271146128950868E-5	0.0	0.0	24.557278090073503	0.0
29	7.271146128950868E-5	0.0	0.0	25.031756730718193	0.0
30	7.271146128950868E-5	0.0	0.0	25.483622106901844	0.0
31	7.271146128950868E-5	0.0	0.0	25.936905356580642	0.0
32	7.271146128950868E-5	0.0	0.0	26.45464731669259	0.0
33	7.271146128950868E-5	0.0	0.0	26.941414194295206	0.0
34	7.271146128950868E-5	0.0	0.0	27.48987674680197	0.0
35	7.271146128950868E-5	0.0	0.0	27.94046967241305	0.0
36	7.271146128950868E-5	0.0	0.0	28.461447292552382	0.0
37	7.271146128950868E-5	0.0	0.0	29.10254424674198	0.0
38	7.271146128950868E-5	0.0	0.0	29.79755674947775	0.0
39	7.271146128950868E-5	0.0	0.0	30.570879496022318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26000	0.0	44.186543	1
ATTGCGG	565	0.0	42.212387	1
CATGCGG	405	0.0	41.666668	1
CACTTAC	5160	0.0	41.511627	36
CGCACTT	5170	0.0	41.431335	34
GCACTTA	5170	0.0	41.431335	35
ACCCGCA	5290	0.0	41.29962	31
CCGCACT	5205	0.0	41.239193	33
CCCGCAC	5305	0.0	41.140434	32
TCACGAC	570	0.0	41.05263	24
TAGTAGG	510	0.0	41.02941	1
ACTTACT	5225	0.0	40.909092	37
CTTACTG	5255	0.0	40.71836	38
CGACGGT	570	0.0	40.657894	27
GCGCGAC	2395	0.0	40.584553	8
TAGGGTA	1105	0.0	40.52036	4
AGGGTAC	1170	0.0	40.384617	5
TTATGAC	5900	0.0	40.00424	26
TATGCGG	445	0.0	39.943817	1
TCAACGC	6155	0.0	39.88221	15
>>END_MODULE