FastQCFastQC Report
Thu 26 May 2016
SRR1043290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043290_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1144787
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503894.40160484002701No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT73230.6396823164483874TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT70300.6140880355909003TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT56250.4913577809671144TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT29360.2564669235412352TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC27520.24039406457271092TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG24050.21008274901793958No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC22550.19697987485881654TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC18480.16142740964039598TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC17890.15627361247114094TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT16150.14107427844655818TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC15230.13303784896229603TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT13070.11416971017315886No Hit
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC12450.10875385552072132TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTTG11890.10386211583464872TruSeq Adapter, Index 19 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC11840.10342535336267794TruSeq Adapter, Index 19 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT2100.046.00000427
ATTACGG1150.046.0000041
ACACGAT253.4171466E-546.031
GTCGAGT206.3114444E-446.08
CCGATCG206.3114444E-446.011
GGTCCAC206.3114444E-446.038
CACGATG253.4171466E-546.032
CGACATC301.8613955E-646.010
TGCATTA206.3114444E-446.040
GTTACGC206.3114444E-446.09
ACGATGG253.4171466E-546.01
ACGATGC253.4171466E-546.033
TCTACGA206.3114444E-446.011
GAGCGTC206.3114444E-446.016
CGGATAT206.3114444E-446.05
ACTACGG600.046.01
GGTATCG253.4171466E-546.07
CGCAGTG405.6115823E-946.041
GACGGCG206.3114444E-446.08
GCGATCC253.4171466E-546.08
Produced by FastQC (version 0.11.5)