##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043290_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1144787
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24322079129131	33.0	31.0	34.0	31.0	34.0
2	32.584033536369645	34.0	31.0	34.0	31.0	34.0
3	32.6834826041875	34.0	31.0	34.0	31.0	34.0
4	36.181546436149254	37.0	35.0	37.0	35.0	37.0
5	36.08409599340314	37.0	35.0	37.0	35.0	37.0
6	36.32687827517259	37.0	37.0	37.0	35.0	37.0
7	36.25894860790697	37.0	37.0	37.0	35.0	37.0
8	36.23335782114926	37.0	37.0	37.0	35.0	37.0
9	37.73173612209084	39.0	38.0	39.0	35.0	39.0
10	37.560126032178914	39.0	37.0	39.0	35.0	39.0
11	37.22680725759464	39.0	37.0	39.0	34.0	39.0
12	37.24118984579664	39.0	37.0	39.0	34.0	39.0
13	37.211976551096406	39.0	37.0	39.0	34.0	39.0
14	38.60993267743257	40.0	38.0	41.0	34.0	41.0
15	38.729853675836644	40.0	38.0	41.0	35.0	41.0
16	38.762842345344595	40.0	38.0	41.0	35.0	41.0
17	38.722737941643295	40.0	38.0	41.0	35.0	41.0
18	38.46578359118334	40.0	38.0	41.0	35.0	41.0
19	38.221720721846076	40.0	37.0	41.0	34.0	41.0
20	37.94824452059641	40.0	35.0	41.0	34.0	41.0
21	37.87159620086531	40.0	35.0	41.0	34.0	41.0
22	37.83985405145236	40.0	35.0	41.0	34.0	41.0
23	37.82181052021031	40.0	35.0	41.0	34.0	41.0
24	37.74923020614315	40.0	35.0	41.0	34.0	41.0
25	37.68088648805411	40.0	35.0	41.0	34.0	41.0
26	37.61470649125121	40.0	35.0	41.0	34.0	41.0
27	37.66839682840563	40.0	35.0	41.0	34.0	41.0
28	37.683636344577636	40.0	35.0	41.0	34.0	41.0
29	37.5886902978458	40.0	35.0	41.0	34.0	41.0
30	37.54471006396823	40.0	35.0	41.0	34.0	41.0
31	37.411269520006776	40.0	35.0	41.0	33.0	41.0
32	37.11165570538449	40.0	35.0	41.0	33.0	41.0
33	36.94652717055662	40.0	35.0	41.0	33.0	41.0
34	36.66360292351328	40.0	35.0	41.0	31.0	41.0
35	36.48440102831356	39.0	35.0	41.0	31.0	41.0
36	36.41094369520269	40.0	35.0	41.0	31.0	41.0
37	36.39744773481879	40.0	35.0	41.0	31.0	41.0
38	36.353394998370874	40.0	35.0	41.0	30.0	41.0
39	36.35038221083922	40.0	35.0	41.0	30.0	41.0
40	36.34992098966882	40.0	35.0	41.0	31.0	41.0
41	36.342293369858325	40.0	35.0	41.0	31.0	41.0
42	36.24768275670496	39.0	35.0	41.0	30.0	41.0
43	36.13804576746591	39.0	35.0	41.0	30.0	41.0
44	35.94913726308911	39.0	35.0	41.0	30.0	41.0
45	35.895291438494674	39.0	35.0	41.0	29.0	41.0
46	35.88053323456678	39.0	35.0	41.0	29.0	41.0
47	35.81235635974203	39.0	35.0	41.0	29.0	41.0
48	35.777638110845075	39.0	35.0	41.0	29.0	41.0
49	35.74781247515914	39.0	35.0	41.0	29.0	41.0
50	35.64278420352432	39.0	35.0	41.0	28.0	41.0
51	35.554502278589815	39.0	35.0	41.0	27.0	41.0
52	35.214948282955696	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	6.0
15	11.0
16	40.0
17	81.0
18	185.0
19	341.0
20	739.0
21	1173.0
22	2012.0
23	3555.0
24	6613.0
25	12302.0
26	17198.0
27	18037.0
28	16170.0
29	15452.0
30	16631.0
31	19501.0
32	24420.0
33	33353.0
34	61916.0
35	110414.0
36	95415.0
37	87330.0
38	146540.0
39	454468.0
40	881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.612210830486372	19.83862500185624	26.519518478109898	26.029645689547486
2	32.800686940015915	20.463806804235197	26.955494777631124	19.78001147811776
3	21.62603174214941	22.111886315969695	37.224916076091006	19.03716586578988
4	23.244149348306717	24.336055528233636	34.61726941343674	17.802525710022913
5	20.13911758257213	30.25348820348239	30.45736892539835	19.150025288547127
6	74.895504578581	1.6583871060730073	19.863782520241756	3.5823257951042424
7	75.94792743104176	1.530765111763149	16.082030980435665	6.439276476759432
8	64.09209748188964	3.2192888284021395	23.98979024045521	8.698823449253005
9	34.920295216490054	18.65604693274819	28.201403405174936	18.22225444558682
10	27.302720942847884	20.47166852873067	40.3582500500093	11.867360478412142
11	24.223807572937147	16.88925538113204	39.26407270522814	19.62286434070268
12	19.08678208260576	21.390092654790806	41.67998064268724	17.843144619916192
13	14.216006995187753	24.371258583474482	40.791867832181886	20.620866589155888
14	12.473674142001961	23.124651135975512	42.75598866863442	21.645686053388097
15	13.558766827366139	18.69133734048343	46.91964531393176	20.830250518218673
16	14.531960967411406	24.66485031713323	37.06244043651788	23.740748278937478
17	16.235247255603007	21.78868208671133	39.78076270956955	22.195307948116113
18	18.055847943765958	27.403351016389948	37.90705170481496	16.633749335029137
19	17.880618840011287	23.277343296176493	35.134658237733305	23.70737962607891
20	14.668580268643861	28.778191925659534	35.16182486348989	21.391402942206717
21	15.473271447002803	23.595393728265606	35.90807722309914	25.023257601632444
22	13.132224597239487	26.41452077984813	32.5290206824501	27.924233940462283
23	12.948784359011764	23.255330467589168	42.83635296347705	20.95953220992202
24	17.675340478185024	21.45962524032855	38.41937408443667	22.445660197049758
25	13.561474754692357	26.35005463898524	37.0981676067251	22.990302999597308
26	15.117397384841022	23.999136957355386	36.34090883282218	24.542556824981414
27	20.121210321221326	23.782502771257885	37.9632193587104	18.133067548810388
28	15.657148447702498	22.855954863219097	35.61955193411526	25.86734475496315
29	18.014792271400708	26.57271614719594	33.81362646501052	21.598865116392833
30	21.265702702773527	22.073625923425055	35.395667490983044	21.265003882818377
31	23.293852917616988	23.360066108367757	31.01004815743016	22.336032816585096
32	15.867405901709224	24.464114285015466	38.77795607392467	20.890523739350638
33	20.729008977215848	21.086630089265515	37.513441365074904	20.67091956844374
34	18.433647482020675	19.191867133361924	38.18063971725745	24.193845667359952
35	22.080177360504617	19.820018920550286	40.213332261809406	17.886471457135695
36	20.601474335400386	20.544869919032973	38.27585393614707	20.57780180941957
37	19.177716029270073	20.011233530779087	40.003598922769044	20.8074515171818
38	16.884800403917936	20.476822325899928	38.62648684864521	24.011890421536933
39	21.642104601117936	19.11281312593522	39.67034915665534	19.5747331162915
40	15.185969092940432	18.758161998694952	42.567918748203816	23.487950160160796
41	18.858879424731413	22.470031542985723	34.618317643369465	24.052771388913396
42	19.695891026016195	17.50404223667809	40.5720016037918	22.22806513351392
43	23.56656740511554	20.658690219228557	35.686551297315575	20.088191078340337
44	16.959661491613723	23.626665921258713	35.53988645922779	23.873786127899777
45	20.105224814747196	20.678693940444816	35.63143187335286	23.58464937145513
46	22.832194984743886	18.90701064914259	35.38658283156605	22.874211534547477
47	16.929000766081376	17.44219667064703	41.45426179717275	24.174540766098847
48	18.775283087596208	15.756380881334255	35.883531172174386	29.584804858895147
49	20.093606932992774	16.71123099755675	39.3647027787702	23.830459290680274
50	18.570965603208283	15.948556369001397	39.883663947965864	25.596814079824455
51	16.847413536317234	15.559488358969833	38.05450271535229	29.53859538936064
52	15.894921937443385	15.46960264223825	46.20780983711381	22.42766558320456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	470.0
1	1719.5
2	2969.0
3	30829.5
4	58690.0
5	33432.5
6	8175.0
7	7980.5
8	7786.0
9	8083.5
10	8381.0
11	8564.0
12	8747.0
13	8511.5
14	7545.5
15	6815.0
16	6338.5
17	5862.0
18	5356.0
19	4850.0
20	4461.0
21	4072.0
22	3715.5
23	3359.0
24	3525.5
25	3692.0
26	4239.0
27	4786.0
28	4845.0
29	4904.0
30	6143.0
31	7382.0
32	9189.0
33	10996.0
34	11947.0
35	12898.0
36	15725.0
37	18552.0
38	21004.0
39	29471.5
40	35487.0
41	48836.5
42	62186.0
43	87193.5
44	112201.0
45	140197.5
46	168194.0
47	172853.0
48	177512.0
49	154970.0
50	132428.0
51	108050.5
52	83673.0
53	66885.0
54	50097.0
55	41172.0
56	32247.0
57	28471.5
58	24696.0
59	20697.0
60	16698.0
61	13917.5
62	11137.0
63	9404.0
64	6151.5
65	4632.0
66	3890.0
67	3148.0
68	2660.0
69	2172.0
70	1868.5
71	1565.0
72	1633.5
73	1702.0
74	1338.5
75	975.0
76	814.5
77	654.0
78	500.0
79	346.0
80	223.0
81	100.0
82	90.5
83	81.0
84	61.0
85	41.0
86	29.5
87	18.0
88	10.5
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1144787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.470524012122844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.96929612505309	20.91502345576834
2	12.172437938943832	7.174562491314386
3	5.233452671467749	4.626977778623962
4	2.778881460229095	3.275803712020981
5	1.7219214086930505	2.5372963110938467
6	1.1075749136481199	1.9584487852735082
7	0.8190710496168423	1.6896917124757467
8	0.5634766201751757	1.3284761012113875
9	0.42091349344813594	1.1164087094120823
>10	3.4067690137975726	22.018252498414544
>50	0.5847600960848058	11.736302650878175
>100	0.20445681462482487	11.04036254339053
>500	0.011921680152798913	2.3670530265979832
>1k	0.0038745460496596466	2.0346871260953017
>5k	8.941260114599185E-4	1.7547584461529
>10k+	2.980420038199728E-4	4.425894651276328
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50389	4.40160484002701	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	7323	0.6396823164483874	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	7030	0.6140880355909003	TruSeq Adapter, Index 13 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	5625	0.4913577809671144	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	2936	0.2564669235412352	TruSeq Adapter, Index 19 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2752	0.24039406457271092	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	2405	0.21008274901793958	No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2255	0.19697987485881654	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1848	0.16142740964039598	TruSeq Adapter, Index 19 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1789	0.15627361247114094	TruSeq Adapter, Index 13 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1615	0.14107427844655818	TruSeq Adapter, Index 13 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1523	0.13303784896229603	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	1307	0.11416971017315886	No Hit
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1245	0.10875385552072132	TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTTG	1189	0.10386211583464872	TruSeq Adapter, Index 19 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1184	0.10342535336267794	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6311217719977603	0.0
2	0.0	0.0	0.0	2.196478471540994	0.0
3	0.0	0.0	0.0	3.6661841897226295	0.0
4	0.0	0.0	0.0	4.611425531561767	0.0
5	0.0	0.0	0.0	9.4863061862163	0.0
6	0.0	0.0	0.0	10.485880779568602	0.0
7	0.0	0.0	0.0	14.176348962732805	0.0
8	0.0	0.0	0.0	18.787687141800177	0.0
9	0.0	0.0	0.0	22.08803908500009	0.0
10	0.0	0.0	0.0	24.37318033835115	0.0
11	0.0	0.0	0.0	26.421159569422084	0.0
12	0.0	0.0	0.0	27.883440325580217	0.0
13	0.0	0.0	0.0	28.49001604665322	0.0
14	0.0	0.0	0.0	28.942938730086908	0.0
15	0.0	0.0	0.0	30.426358789888425	0.0
16	0.0	0.0	0.0	32.19542150635883	0.0
17	0.0	0.0	0.0	34.13883980163995	0.0
18	0.0	0.0	0.0	35.20244377338317	0.0
19	0.0	0.0	0.0	36.270240664857305	0.0
20	0.0	0.0	0.0	37.543577975640886	0.0
21	0.0	0.0	0.0	38.77498608911527	0.0
22	5.24114966364922E-4	0.0	0.0	39.96498912024682	0.0
23	5.24114966364922E-4	0.0	0.0	40.904639902444735	0.0
24	0.0012229349215181515	0.0	0.0	41.65552194425688	0.0
25	0.0012229349215181515	0.0	0.0	42.39408728435945	0.0
26	0.0012229349215181515	0.0	0.0	43.16995213956832	0.0
27	0.0012229349215181515	0.0	0.0	43.900218992703444	0.0
28	0.0013976399103064587	0.0	0.0	44.645947237346334	0.0
29	0.0013976399103064587	0.0	0.0	45.47841650892262	0.0
30	0.0014849924047006125	0.0	0.0	46.26589924588592	0.0
31	0.0014849924047006125	0.0	0.0	46.93222407312452	0.0
32	0.0014849924047006125	0.0	0.0	47.59662714548645	0.0
33	0.0014849924047006125	0.0	0.0	48.2384932742947	0.0
34	0.0014849924047006125	0.0	0.0	48.97583567947574	0.0
35	0.0014849924047006125	0.0	0.0	49.59376722481999	0.0
36	0.0014849924047006125	0.0	0.0	50.1627813733035	0.0
37	0.0015723448990947661	0.0	0.0	50.76935709437651	0.0
38	0.0015723448990947661	0.0	0.0	51.373836355584054	0.0
39	0.0015723448990947661	0.0	0.0	51.953682213372446	0.0
40	0.0016596973934889197	0.0	0.0	52.5788640157514	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	210	0.0	46.000004	27
ATTACGG	115	0.0	46.000004	1
ACACGAT	25	3.4171466E-5	46.0	31
GTCGAGT	20	6.3114444E-4	46.0	8
CCGATCG	20	6.3114444E-4	46.0	11
GGTCCAC	20	6.3114444E-4	46.0	38
CACGATG	25	3.4171466E-5	46.0	32
CGACATC	30	1.8613955E-6	46.0	10
TGCATTA	20	6.3114444E-4	46.0	40
GTTACGC	20	6.3114444E-4	46.0	9
ACGATGG	25	3.4171466E-5	46.0	1
ACGATGC	25	3.4171466E-5	46.0	33
TCTACGA	20	6.3114444E-4	46.0	11
GAGCGTC	20	6.3114444E-4	46.0	16
CGGATAT	20	6.3114444E-4	46.0	5
ACTACGG	60	0.0	46.0	1
GGTATCG	25	3.4171466E-5	46.0	7
CGCAGTG	40	5.6115823E-9	46.0	41
GACGGCG	20	6.3114444E-4	46.0	8
GCGATCC	25	3.4171466E-5	46.0	8
>>END_MODULE