FastQCFastQC Report
Thu 26 May 2016
SRR1043289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043289_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1135105
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT478154.212385638333018No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC71560.6304262601257152TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT69450.611837671404848TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC58430.5147541416873329TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC29270.2578616075164853TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG27780.24473506856193922No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC22830.20112676800824592No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG22450.1977790600869523No Hit
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG19770.17416890948414462No Hit
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG18020.15875183353081873No Hit
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG15680.1381370005418001No Hit
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC15230.13417260958237343TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT14730.12976773073856604TruSeq Adapter, Index 19 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG12810.11285299597834561No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC12470.10985767836455658No Hit
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG12430.109505288057052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACA302.1654778E-645.0000049
TTATCCG302.1654778E-645.00000412
TAGCCGG302.1654778E-645.0000041
TGCTCGT302.1654778E-645.00000410
CCAACGG600.045.0000041
ATACCGG302.1654778E-645.0000041
CTAGCGC302.1654778E-645.0000041
AGCACGG1450.045.0000041
AAACTCG207.0332503E-445.023
ACTGCGA207.0332503E-445.015
TTTACGG207.0332503E-445.01
ATTACGG950.045.01
TTCACTC207.0332503E-445.019
TTCACGG950.045.01
GCACCTT207.0332503E-445.039
CTAACGG750.045.01
GTGCTCG406.8157533E-945.09
TAATTCG207.0332503E-445.025
CAAGCGA207.0332503E-445.017
CGCACTC207.0332503E-445.025
Produced by FastQC (version 0.11.5)