##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043289_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1135105
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20640557481467	33.0	31.0	34.0	31.0	34.0
2	32.4999634395056	34.0	31.0	34.0	31.0	34.0
3	32.58139907761837	34.0	31.0	34.0	31.0	34.0
4	36.13669220028103	37.0	35.0	37.0	35.0	37.0
5	36.09656375401394	37.0	35.0	37.0	35.0	37.0
6	36.326397117447286	37.0	37.0	37.0	35.0	37.0
7	36.267564674633626	37.0	37.0	37.0	35.0	37.0
8	36.16735720484008	37.0	37.0	37.0	35.0	37.0
9	37.68034939498989	39.0	38.0	39.0	35.0	39.0
10	37.50227159601975	39.0	37.0	39.0	35.0	39.0
11	37.21956030499381	39.0	37.0	39.0	34.0	39.0
12	37.23974698375921	39.0	37.0	39.0	34.0	39.0
13	37.22052937833945	39.0	37.0	39.0	34.0	39.0
14	38.61706361966514	40.0	38.0	41.0	35.0	41.0
15	38.74888578589646	40.0	38.0	41.0	35.0	41.0
16	38.80119812704552	40.0	38.0	41.0	35.0	41.0
17	38.74425449628008	40.0	38.0	41.0	35.0	41.0
18	38.52122138480581	40.0	38.0	41.0	35.0	41.0
19	38.29246985961651	40.0	37.0	41.0	35.0	41.0
20	38.044828451993425	40.0	35.0	41.0	34.0	41.0
21	37.97280956387294	40.0	35.0	41.0	34.0	41.0
22	37.94828936530101	40.0	35.0	41.0	34.0	41.0
23	37.905560278564536	40.0	35.0	41.0	34.0	41.0
24	37.83806343906511	40.0	35.0	41.0	34.0	41.0
25	37.82404975751142	40.0	35.0	41.0	34.0	41.0
26	37.79254782597204	40.0	35.0	41.0	34.0	41.0
27	37.804041035851306	40.0	35.0	41.0	34.0	41.0
28	37.75510195092084	40.0	35.0	41.0	34.0	41.0
29	37.72052541394849	40.0	35.0	41.0	34.0	41.0
30	37.64405583624423	40.0	35.0	41.0	34.0	41.0
31	37.497345179520835	40.0	35.0	41.0	34.0	41.0
32	37.21046334920558	40.0	35.0	41.0	33.0	41.0
33	37.02482149228486	40.0	35.0	41.0	33.0	41.0
34	36.70356927332714	40.0	35.0	41.0	32.0	41.0
35	36.54316561022989	40.0	35.0	41.0	31.0	41.0
36	36.452739614396904	40.0	35.0	41.0	31.0	41.0
37	36.3985393421754	39.0	35.0	41.0	31.0	41.0
38	36.35466058206069	39.0	35.0	41.0	30.0	41.0
39	36.296585778408165	39.0	35.0	41.0	30.0	41.0
40	36.26976270917668	39.0	35.0	41.0	30.0	41.0
41	36.21942287277388	39.0	35.0	41.0	30.0	41.0
42	36.11759440756582	39.0	35.0	41.0	30.0	41.0
43	35.989693464481256	39.0	35.0	41.0	30.0	41.0
44	35.882826698851645	39.0	35.0	41.0	29.0	41.0
45	35.8378801961052	39.0	35.0	41.0	29.0	41.0
46	35.75020020174345	39.0	35.0	41.0	29.0	41.0
47	35.62752696887072	38.0	35.0	40.0	28.0	41.0
48	35.61980785918483	38.0	35.0	40.0	28.0	41.0
49	35.5739530704208	38.0	35.0	40.0	28.0	41.0
50	35.49131137648059	38.0	35.0	40.0	27.0	41.0
51	35.05312988666247	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	1.0
15	6.0
16	27.0
17	64.0
18	143.0
19	329.0
20	635.0
21	1005.0
22	1690.0
23	2903.0
24	5353.0
25	10267.0
26	16031.0
27	18593.0
28	17191.0
29	16010.0
30	16971.0
31	19522.0
32	24301.0
33	32872.0
34	62455.0
35	109228.0
36	92081.0
37	88426.0
38	155823.0
39	443084.0
40	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.071588090969556	19.842569630122323	26.543535620052772	25.542306658855345
2	32.56817651230503	20.464802815598556	27.055558736856945	19.911461935239473
3	21.718607529699895	22.255033675298762	36.80743191158527	19.21892688341607
4	23.429286277480937	24.530065500548407	34.18758617044238	17.853062051528273
5	20.367454993150414	30.31146898304562	30.049290594262207	19.27178542954176
6	75.15569044273437	1.631919514053766	19.56021689623427	3.652173146977592
7	76.23920254073411	1.4625078737209334	15.714405275282903	6.583884310262047
8	64.10525898485162	3.169750816003806	23.82766352011488	8.897326679029693
9	35.140978147396055	18.581188524409637	27.894688156602253	18.383145171592055
10	27.428475779773677	20.39176992436823	40.23337048114492	11.946383814713176
11	24.271851502724417	16.893855634500774	38.98546830469428	19.84882455808053
12	19.18650697512565	21.26772413124777	41.61623814536981	17.92953074825677
13	14.285198285621153	24.289030530215268	40.702842468317904	20.722928715845672
14	12.546504508393497	23.129930711255785	42.524700358116654	21.798864422234065
15	13.555397958779144	18.666555076402624	46.78377771219402	20.994269252624207
16	14.514780570960395	24.750573735469406	36.98582950475947	23.748816188810725
17	16.329590654609046	21.790847542738337	39.501543910034755	22.378017892617862
18	18.062469991762878	27.58960624788015	37.66374035882143	16.68418340153554
19	17.912704111073424	23.292823130899784	34.88329273503332	23.911180022993467
20	14.637676690702623	28.94428268750468	34.88619995507023	21.531840666722463
21	15.479977623215474	23.620986604763434	35.66780165711542	25.23123411490567
22	13.196576528162593	26.40592720497223	32.17015165997859	28.22734460688659
23	12.97871122054788	23.236616876852803	42.656846723430874	21.127825179168447
24	17.79456526048251	21.454314799071454	38.08308482475189	22.668035115694142
25	13.57495562084565	26.408658229855387	36.94829993701023	23.06808621228873
26	15.310037397421382	23.85920245263654	36.099039295924165	24.73172085401791
27	20.33230405997683	23.72080115936411	37.70223899991631	18.24465578074275
28	15.77008294386863	22.84828275798274	35.267310072636455	26.114324225512174
29	18.231088753903823	26.591284506719642	33.409244078741615	21.76838266063492
30	21.425154501125444	21.98739323674902	35.06415706036006	21.523295201765475
31	23.54381312741993	23.35396284925183	30.60721254861885	22.49501147470939
32	15.97499790768255	24.39236898789099	38.593786477902924	21.03884662652354
33	21.029684478528416	20.912690896436896	37.27355619083697	20.784068434197717
34	18.649640341642403	18.91569502380837	38.09048502120949	24.344179613339737
35	22.26692684817704	19.548764211240368	40.293541126151325	17.890767814431264
36	20.7616035520943	20.312746397910324	38.19047577096392	20.735174279031455
37	19.39776496447465	19.725928438338304	40.008721660110744	20.867584937076305
38	16.999572726752152	20.103514652829475	38.64981653679615	24.247096083622218
39	21.690504402676407	18.81552807890019	39.746719466481075	19.747248051942332
40	15.186260301910396	18.439439523215913	42.73904176265631	23.63525841221737
41	19.01401191960215	22.340752617599254	34.55548165147718	24.08975381132142
42	19.847238801696758	17.25232467480982	40.532373657062564	22.36806286643086
43	23.826606349192364	20.497310821465856	35.52869558322798	20.147387246113794
44	17.188453931574614	23.26586527237568	35.540764951260016	24.00491584478969
45	20.253456728672678	20.396527193519542	35.60630954845587	23.74370652935191
46	23.07090533474877	18.59325789244167	35.314970861726444	23.020865911083117
47	17.109254209962955	17.14185031340713	41.39211790979689	24.356777566833024
48	18.886710921016117	15.431259663202965	35.88126208588633	29.80076732989459
49	20.26596658458909	16.34069094929544	39.35204232207593	24.04130014403954
50	18.68946044639042	15.637760383400654	39.94396994110677	25.72880922910215
51	17.07093176402183	15.306513494346339	37.79403667502125	29.828518066610577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	381.0
1	1576.5
2	2772.0
3	29245.0
4	55718.0
5	32149.0
6	8580.0
7	8144.0
8	7708.0
9	8039.5
10	8371.0
11	8405.5
12	8440.0
13	8010.5
14	7581.0
15	7002.5
16	6424.0
17	5846.0
18	5268.0
19	4792.0
20	4316.0
21	3978.0
22	3640.0
23	3278.0
24	2916.0
25	3304.0
26	4194.5
27	4697.0
28	4905.0
29	5113.0
30	6638.5
31	8164.0
32	9765.5
33	11367.0
34	12736.0
35	14105.0
36	17081.5
37	20058.0
38	24107.5
39	28157.0
40	36586.5
41	45016.0
42	58764.0
43	72512.0
44	100808.5
45	129105.0
46	156794.5
47	184484.0
48	181387.5
49	178291.0
50	144820.5
51	111350.0
52	89216.0
53	67082.0
54	53609.0
55	40136.0
56	34313.5
57	28491.0
58	24852.0
59	21213.0
60	17152.0
61	13091.0
62	10958.0
63	8825.0
64	7300.0
65	5775.0
66	4741.5
67	3708.0
68	3177.5
69	2647.0
70	2189.5
71	1732.0
72	1669.0
73	1606.0
74	1345.0
75	867.5
76	651.0
77	562.5
78	474.0
79	338.5
80	203.0
81	138.0
82	73.0
83	57.5
84	42.0
85	36.5
86	31.0
87	17.0
88	3.0
89	4.5
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	2.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1135105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.845906724121086
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.25839432529696	20.26667089294034
2	12.211802055382568	7.0452100604599135
3	5.464112114658846	4.728518051687672
4	2.938909307355643	3.3910201500252866
5	1.7747046909365154	2.559648298880743
6	1.126136717382702	1.9490660824937593
7	0.7988928431711544	1.6131351905678109
8	0.5905447549525346	1.3627839134223803
9	0.4670075158518339	1.212412971755295
>10	3.5492866772686344	22.455860094136508
>50	0.5886862686101626	11.514435075525292
>100	0.21392700171864337	11.364284849140615
>500	0.012347878886954565	2.4198370247577445
>1k	0.0040130606382602334	2.0834195719689768
>5k	9.260909165215924E-4	1.7759422123925834
>10k+	3.086969721738641E-4	4.257755559845135
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	47815	4.212385638333018	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	7156	0.6304262601257152	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	6945	0.611837671404848	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	5843	0.5147541416873329	TruSeq Adapter, Index 19 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2927	0.2578616075164853	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	2778	0.24473506856193922	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	2283	0.20112676800824592	No Hit
ACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	2245	0.1977790600869523	No Hit
GCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1977	0.17416890948414462	No Hit
AGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1802	0.15875183353081873	No Hit
TCCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1568	0.1381370005418001	No Hit
ACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	1523	0.13417260958237343	TruSeq Adapter, Index 13 (95% over 21bp)
TGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCTT	1473	0.12976773073856604	TruSeq Adapter, Index 19 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1281	0.11285299597834561	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	1247	0.10985767836455658	No Hit
GGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTG	1243	0.109505288057052	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6288405037419446	0.0
2	0.0	0.0	0.0	2.2101039110919256	0.0
3	0.0	0.0	0.0	3.7260870139766804	0.0
4	0.0	0.0	0.0	4.693574603230538	0.0
5	0.0	0.0	0.0	9.67637355134547	0.0
6	0.0	0.0	0.0	10.709493835372058	0.0
7	0.0	0.0	0.0	14.522973645609877	0.0
8	0.0	0.0	0.0	19.261478013047252	0.0
9	0.0	0.0	0.0	22.676668678228005	0.0
10	0.0	0.0	0.0	25.037683738508772	0.0
11	0.0	0.0	0.0	27.137401385774883	0.0
12	0.0	0.0	0.0	28.61312389602724	0.0
13	0.0	0.0	0.0	29.230071226890903	0.0
14	0.0	0.0	0.0	29.685183309033086	0.0
15	0.0	0.0	0.0	31.18812797054017	0.0
16	0.0	0.0	0.0	33.004435712995715	0.0
17	0.0	0.0	0.0	34.99218134005224	0.0
18	0.0	0.0	0.0	36.09172719704345	0.0
19	0.0	0.0	0.0	37.18307997938517	0.0
20	0.0	0.0	0.0	38.4575876240524	0.0
21	0.0	0.0	0.0	39.70945419146246	0.0
22	3.523903075045921E-4	0.0	0.0	40.92696270389083	0.0
23	3.523903075045921E-4	0.0	0.0	41.866963849159326	0.0
24	0.0016738539606468124	0.0	0.0	42.62407442483295	0.0
25	0.0016738539606468124	0.0	0.0	43.36858704701327	0.0
26	0.0016738539606468124	0.0	0.0	44.147722016905924	0.0
27	0.0016738539606468124	0.0	0.0	44.88677258931993	0.0
28	0.0018500491143991086	0.0	0.0	45.64908092202924	0.0
29	0.0018500491143991086	0.0	0.0	46.49199853758022	0.0
30	0.0018500491143991086	0.0	0.0	47.29897234176574	0.0
31	0.0018500491143991086	0.0	0.0	47.966928169640696	0.0
32	0.0018500491143991086	0.0	0.0	48.650917756507106	0.0
33	0.0018500491143991086	0.0	0.0	49.29209192101171	0.0
34	0.0018500491143991086	0.0	0.0	50.03889508019082	0.0
35	0.0018500491143991086	0.0	0.0	50.66579743724149	0.0
36	0.0018500491143991086	0.0	0.0	51.23649354024518	0.0
37	0.0018500491143991086	0.0	0.0	51.850709846225676	0.0
38	0.0018500491143991086	0.0	0.0	52.46316420066866	0.0
39	0.0018500491143991086	0.0	0.0	53.04284625651371	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACA	30	2.1654778E-6	45.000004	9
TTATCCG	30	2.1654778E-6	45.000004	12
TAGCCGG	30	2.1654778E-6	45.000004	1
TGCTCGT	30	2.1654778E-6	45.000004	10
CCAACGG	60	0.0	45.000004	1
ATACCGG	30	2.1654778E-6	45.000004	1
CTAGCGC	30	2.1654778E-6	45.000004	1
AGCACGG	145	0.0	45.000004	1
AAACTCG	20	7.0332503E-4	45.0	23
ACTGCGA	20	7.0332503E-4	45.0	15
TTTACGG	20	7.0332503E-4	45.0	1
ATTACGG	95	0.0	45.0	1
TTCACTC	20	7.0332503E-4	45.0	19
TTCACGG	95	0.0	45.0	1
GCACCTT	20	7.0332503E-4	45.0	39
CTAACGG	75	0.0	45.0	1
GTGCTCG	40	6.8157533E-9	45.0	9
TAATTCG	20	7.0332503E-4	45.0	25
CAAGCGA	20	7.0332503E-4	45.0	17
CGCACTC	20	7.0332503E-4	45.0	25
>>END_MODULE