FastQCFastQC Report
Thu 26 May 2016
SRR1043288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043288_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences492743
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT304586.1813156148336965No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT36940.7496808681198921No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT33840.6867677470811356Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT29430.597268758764711No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT14680.2979240699512728No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC12140.24637589980984004No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG11050.22425483467040627No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC10870.22060181473912366No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC10110.20517795280704137No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC9010.18285394211586972No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC8380.1700683723563805No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT8160.16560357021814617No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC6290.12765275204315435No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT5740.11649074669756851No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG5590.11344656342149964Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG5510.11182299900759625No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC5500.11162005345585833No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.10735819686936192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG351.0179792E-746.0000041
ATCACGG351.0179792E-746.0000041
TTGACGG351.0179792E-746.0000041
ACTACGG351.0179792E-746.0000041
GCGATAC351.0179792E-746.0000048
ATCGGGT351.0179792E-746.0000043
ATTGCGG351.0179792E-746.0000041
AGTCGTA351.0179792E-746.0000044
CTTAAGG1050.046.01
CGGGTTC206.3079264E-446.05
AACAAGG405.6006684E-946.01
AATCCGG206.3079264E-446.01
CTGTCGG253.4142937E-546.01
CCGGGAC253.4142937E-546.04
CTGTATG253.4142937E-546.017
ATAGGTG253.4142937E-546.023
CTCAAGG301.8592236E-646.01
TACCAGG253.4142937E-546.01
CGCGGGT501.6370905E-1146.03
GGATGCG206.3079264E-446.08
Produced by FastQC (version 0.11.5)