##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043288_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	492743
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21493963384564	33.0	31.0	34.0	30.0	34.0
2	32.53892394209557	34.0	31.0	34.0	31.0	34.0
3	32.61338060611718	34.0	31.0	34.0	31.0	34.0
4	36.13194505046241	37.0	35.0	37.0	35.0	37.0
5	36.02591817641245	37.0	35.0	37.0	35.0	37.0
6	36.286916303225006	37.0	37.0	37.0	35.0	37.0
7	36.21369151870245	37.0	37.0	37.0	35.0	37.0
8	36.19396926998456	37.0	37.0	37.0	35.0	37.0
9	37.73547265004272	39.0	38.0	39.0	35.0	39.0
10	37.5788291259338	39.0	37.0	39.0	35.0	39.0
11	37.164195128089084	39.0	37.0	39.0	34.0	39.0
12	37.075536334356855	39.0	37.0	39.0	33.0	39.0
13	37.08966337421333	39.0	37.0	39.0	33.0	39.0
14	38.49600095790301	40.0	37.0	41.0	34.0	41.0
15	38.619101641220674	40.0	37.0	41.0	35.0	41.0
16	38.65677442398979	40.0	37.0	41.0	35.0	41.0
17	38.653143728069196	40.0	37.0	41.0	35.0	41.0
18	38.38563307850137	40.0	37.0	41.0	35.0	41.0
19	38.10383709154671	40.0	37.0	41.0	34.0	41.0
20	37.792934653561794	39.0	35.0	41.0	34.0	41.0
21	37.71206085119423	39.0	35.0	41.0	34.0	41.0
22	37.67577621599901	39.0	35.0	41.0	34.0	41.0
23	37.660250881291056	39.0	35.0	41.0	34.0	41.0
24	37.56552401556186	39.0	35.0	41.0	34.0	41.0
25	37.471771288481015	39.0	35.0	41.0	34.0	41.0
26	37.426242483404124	39.0	35.0	41.0	34.0	41.0
27	37.46924867527291	39.0	35.0	41.0	34.0	41.0
28	37.50490417925775	39.0	35.0	41.0	34.0	41.0
29	37.45831599840079	39.0	35.0	41.0	34.0	41.0
30	37.39658807938418	39.0	35.0	41.0	33.0	41.0
31	37.24609177603741	39.0	35.0	41.0	33.0	41.0
32	37.09181053815072	39.0	35.0	41.0	33.0	41.0
33	36.88306277308861	39.0	35.0	41.0	33.0	41.0
34	36.698213876199155	39.0	35.0	41.0	32.0	41.0
35	36.60009984921145	39.0	35.0	41.0	32.0	41.0
36	36.5171012069172	39.0	35.0	41.0	31.0	41.0
37	36.49000391684915	39.0	35.0	41.0	31.0	41.0
38	36.45952961280018	39.0	35.0	41.0	31.0	41.0
39	36.4390382004412	39.0	35.0	41.0	31.0	41.0
40	36.39283155722151	39.0	35.0	41.0	31.0	41.0
41	36.374369600379914	39.0	35.0	41.0	31.0	41.0
42	36.28634602622462	39.0	35.0	41.0	31.0	41.0
43	36.1547500421112	39.0	35.0	41.0	31.0	41.0
44	35.96973472986932	39.0	35.0	41.0	30.0	41.0
45	35.90400675402796	38.0	35.0	41.0	30.0	41.0
46	35.90551869838841	38.0	35.0	41.0	30.0	41.0
47	35.84189729737409	38.0	35.0	41.0	30.0	41.0
48	35.82516646608881	38.0	35.0	40.0	30.0	41.0
49	35.779584895168476	38.0	35.0	41.0	30.0	41.0
50	35.631820238948094	38.0	35.0	40.0	29.0	41.0
51	35.57016132141908	38.0	35.0	40.0	29.0	41.0
52	35.259543007206595	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	3.0
15	9.0
16	15.0
17	32.0
18	83.0
19	186.0
20	343.0
21	550.0
22	811.0
23	1339.0
24	2513.0
25	4525.0
26	6382.0
27	6919.0
28	6483.0
29	6466.0
30	7279.0
31	8577.0
32	10728.0
33	14573.0
34	28465.0
35	58135.0
36	48348.0
37	35338.0
38	58234.0
39	185875.0
40	531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.464099946625318	20.03397308536093	25.738366653610502	27.763560314403247
2	32.48569741224127	20.314646783414478	27.13016724742919	20.069488556915065
3	20.96447844007931	22.62660250881291	37.391906125505585	19.017012925602188
4	22.49955859342497	24.56615314677225	35.18893216139042	17.74535609841236
5	19.561718786466777	29.62172978611568	31.201255015291945	19.61529641212559
6	72.73081504963034	1.599616838798319	21.629936904227964	4.0396312073433815
7	73.51662022595957	1.430563194200628	17.443576062978064	7.609240516861732
8	60.49847486417869	3.075639836588242	26.59195564422021	9.833929655012856
9	33.888254120302065	18.911887129801947	29.236945020020578	17.962913729875414
10	27.869700837962185	18.29736799913951	42.279849739113494	11.553081423784812
11	24.506081263457826	16.072881806540124	39.257584582632326	20.163452347369724
12	16.82499802128087	23.463955855283587	42.70826779883225	17.002778324603295
13	11.02542298926621	27.52124332562817	41.911503562709164	19.541830122396462
14	9.385419985672044	22.6217318155712	48.21336883527518	19.779479363481574
15	9.67137026807078	18.507213699636523	51.3584160505578	20.4629999817349
16	10.563113022407219	24.37274603596601	38.97691088457877	26.087230057047993
17	13.308966337421332	21.399593703005422	42.04382406244228	23.247615897130956
18	15.388955297183319	28.558295095008962	39.58899466862035	16.463754939187368
19	16.49784979187934	22.937718039627146	35.79594230663855	24.768489861854963
20	11.986776067848757	30.929105030411392	35.679451559940986	21.40466734179887
21	13.227991062277903	24.11865820519013	35.550581134587404	27.102769597944565
22	10.811721323286177	27.822820415510723	31.933685511514113	29.431772749688985
23	10.55702465585508	23.135589952571625	44.732649677418046	21.57473571415525
24	16.040816409365533	21.496195785632672	40.374596899397865	22.088390905603937
25	10.809083031113582	27.66756706843121	38.301102197291485	23.222247703163717
26	13.794817988281924	25.61233746598125	35.46473516620226	25.128109379534564
27	18.740601084135136	24.47340702962802	37.80835039767181	18.977641488565034
28	12.191142238448846	25.212331783505803	33.33340098185058	29.263124996194772
29	14.463320635706648	30.258167036365812	30.479377687760152	24.799134640167388
30	19.330157911933807	25.017909944940868	27.13645855953306	28.51547358359226
31	17.240021674584927	32.24946879001833	26.995411401075202	23.515098134321544
32	13.637129294581557	28.307251447509145	27.353813245444382	30.701806012464917
33	10.74576401897135	32.27747527615816	29.17809081001658	27.798669894853912
34	10.835060061736037	30.39434350158196	30.213924906086948	28.556671530595057
35	14.73080287289723	28.01379217969611	27.831547074235452	29.423857873171205
36	13.218655566897958	31.745352039501324	32.34850621926643	22.687486174334285
37	12.79510820042091	31.049248797040242	32.322529188643976	23.833113813894872
38	12.1272143896514	27.094042939219836	32.0229815542788	28.75576111684996
39	18.992253568290163	23.28292842313336	35.716590595909025	22.008227412667456
40	11.789107100456018	20.671425063369746	40.72508386724925	26.814383968924975
41	16.67360063968438	25.34079631775591	30.639907619184847	27.34569542337486
42	17.40501640814786	18.505996026326095	36.39848764974845	27.690499915777593
43	20.991267252908717	24.29380021633996	33.216707289601274	21.498225241150053
44	12.983847563537179	28.317398725096044	33.18504778353016	25.51370592783662
45	17.09674211505795	25.466216668729945	32.36413302675025	25.072908189461852
46	21.104713816330218	21.898839760280715	32.48752392220691	24.508922501182155
47	14.984484812569635	18.743645267411207	38.225403506493244	28.046466413525916
48	16.890143543388746	16.738746161792253	33.487233710067926	32.88387658475108
49	18.732280316513883	18.299803345760367	38.23453605632145	24.7333802814043
50	16.5640100417459	17.664583768820663	38.294810885187616	27.476595304245826
51	14.644753958960349	16.680703733995205	37.557915586827214	31.116626720217234
52	14.046673417988686	15.533452530020723	46.59203682244091	23.827837229549683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	209.0
1	876.5
2	1544.0
3	17236.0
4	32928.0
5	17865.0
6	2802.0
7	2818.5
8	2835.0
9	3071.0
10	3307.0
11	3438.0
12	3569.0
13	3482.0
14	3177.0
15	2959.0
16	2637.0
17	2315.0
18	2130.0
19	1945.0
20	1839.0
21	1733.0
22	1604.5
23	1476.0
24	1597.5
25	1719.0
26	1801.5
27	1884.0
28	1900.5
29	1917.0
30	2285.5
31	2654.0
32	2908.5
33	3163.0
34	4265.5
35	5368.0
36	6516.0
37	7664.0
38	8503.5
39	12010.0
40	14677.0
41	21748.5
42	28820.0
43	41426.5
44	54033.0
45	67055.5
46	80078.0
47	81210.0
48	82342.0
49	68227.0
50	54112.0
51	41899.0
52	29686.0
53	22979.0
54	16272.0
55	13460.5
56	10649.0
57	9656.0
58	8663.0
59	7412.5
60	6162.0
61	5069.0
62	3976.0
63	3254.5
64	2157.0
65	1781.0
66	1446.0
67	1111.0
68	981.5
69	852.0
70	802.5
71	753.0
72	747.5
73	742.0
74	599.0
75	456.0
76	309.5
77	163.0
78	121.0
79	79.0
80	68.0
81	57.0
82	31.5
83	6.0
84	4.0
85	2.0
86	3.5
87	5.0
88	4.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	492743.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.80513411566371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40961717406213	22.613931024265142
2	11.462941286855907	7.062348874031501
3	4.549160437623701	4.204124921840086
4	2.3011929010260808	2.8355422376848667
5	1.3243308533529616	2.0398094775524673
6	0.9262627335083681	1.7120188639239933
7	0.6147056302091533	1.325526256617258
8	0.48931709125244827	1.2058782896894502
9	0.37320862506455144	1.0347067573412365
>10	3.909681952609398	26.671494180630546
>50	0.4537257308196707	9.280089699439696
>100	0.1739502437399029	9.356291283464778
>500	0.0059526699378674	1.211686685019164
>1k	0.0052912621669932445	3.24080854412558
>5k	0.0	0.0
>10k+	6.614077708741556E-4	6.205742904374256
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30458	6.1813156148336965	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	3694	0.7496808681198921	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	3384	0.6867677470811356	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	2943	0.597268758764711	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	1468	0.2979240699512728	No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1214	0.24637589980984004	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCG	1105	0.22425483467040627	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1087	0.22060181473912366	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1011	0.20517795280704137	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	901	0.18285394211586972	No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	838	0.1700683723563805	No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	816	0.16560357021814617	No Hit
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	629	0.12765275204315435	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	574	0.11649074669756851	No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTTG	559	0.11344656342149964	Illumina Single End Adapter 1 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	551	0.11182299900759625	No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	550	0.11162005345585833	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.10735819686936192	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.701582772358004	0.0
2	0.0	0.0	0.0	2.5546786052769903	0.0
3	0.0	0.0	0.0	4.239735521356975	0.0
4	0.0	0.0	0.0	5.352080090432538	0.0
5	0.0	0.0	0.0	11.072506357269408	0.0
6	0.0	0.0	0.0	12.299312217525161	0.0
7	0.0	0.0	0.0	16.944735896806247	0.0
8	0.0	0.0	0.0	22.63451738533069	0.0
9	0.0	0.0	0.0	26.85903199030732	0.0
10	0.0	0.0	0.0	29.272257546022978	0.0
11	0.0	0.0	0.0	31.457372301585206	0.0
12	0.0	0.0	0.0	33.04805953610706	0.0
13	0.0	0.0	0.0	33.64553124042351	0.0
14	0.0	0.0	0.0	34.08612603324654	0.0
15	0.0	0.0	0.0	35.608826507936186	0.0
16	0.0	0.0	0.0	37.31133674146563	0.0
17	0.0	0.0	0.0	39.54779672161756	0.0
18	0.0	0.0	0.0	40.43933653040226	0.0
19	0.0	0.0	0.0	41.56304605037514	0.0
20	0.0	0.0	0.0	42.642310494517425	0.0
21	0.0	0.0	0.0	43.57443941364971	0.0
22	2.0294555173792423E-4	0.0	0.0	44.53619838333574	0.0
23	2.0294555173792423E-4	0.0	0.0	45.23351929910724	0.0
24	0.0032471288278067877	0.0	0.0	45.82571441907851	0.0
25	0.0032471288278067877	0.0	0.0	46.38239406749563	0.0
26	0.0032471288278067877	0.0	0.0	46.93400007711931	0.0
27	0.0032471288278067877	0.0	0.0	47.46571742267267	0.0
28	0.0032471288278067877	0.0	0.0	48.00169662481253	0.0
29	0.0032471288278067877	0.0	0.0	48.7239798434478	0.0
30	0.0032471288278067877	0.0	0.0	49.32733696876465	0.0
31	0.0032471288278067877	0.0	0.0	49.79329995555492	0.0
32	0.0032471288278067877	0.0	0.0	50.262713016724746	0.0
33	0.0032471288278067877	0.0	0.0	50.73415553341194	0.0
34	0.0032471288278067877	0.0	0.0	51.32574181672799	0.0
35	0.0032471288278067877	0.0	0.0	51.7785133426553	0.0
36	0.0032471288278067877	0.0	0.0	52.22925541306523	0.0
37	0.0032471288278067877	0.0	0.0	52.69217421657943	0.0
38	0.0032471288278067877	0.0	0.0	53.14880170798976	0.0
39	0.0032471288278067877	0.0	0.0	53.576813876605044	0.0
40	0.0032471288278067877	0.0	0.0	54.02491765484238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCGG	35	1.0179792E-7	46.000004	1
ATCACGG	35	1.0179792E-7	46.000004	1
TTGACGG	35	1.0179792E-7	46.000004	1
ACTACGG	35	1.0179792E-7	46.000004	1
GCGATAC	35	1.0179792E-7	46.000004	8
ATCGGGT	35	1.0179792E-7	46.000004	3
ATTGCGG	35	1.0179792E-7	46.000004	1
AGTCGTA	35	1.0179792E-7	46.000004	4
CTTAAGG	105	0.0	46.0	1
CGGGTTC	20	6.3079264E-4	46.0	5
AACAAGG	40	5.6006684E-9	46.0	1
AATCCGG	20	6.3079264E-4	46.0	1
CTGTCGG	25	3.4142937E-5	46.0	1
CCGGGAC	25	3.4142937E-5	46.0	4
CTGTATG	25	3.4142937E-5	46.0	17
ATAGGTG	25	3.4142937E-5	46.0	23
CTCAAGG	30	1.8592236E-6	46.0	1
TACCAGG	25	3.4142937E-5	46.0	1
CGCGGGT	50	1.6370905E-11	46.0	3
GGATGCG	20	6.3079264E-4	46.0	8
>>END_MODULE