FastQCFastQC Report
Thu 26 May 2016
SRR1043287_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043287_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences485731
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284315.853239756161332No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC36450.7504153533540169No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT33210.6837117663892154No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC29330.6038321622461815No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC14120.29069587899475224No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG12010.24725619736026733No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG10680.2198747866617531No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC9690.19949313508917485No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG9480.19516975445256737No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG9460.1947580039157476No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC8230.16943534590133222No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG8020.1651119652647247No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT7020.14452443842373658Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG6410.13196604705073384No Hit
CGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT5890.12126053309342003No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC5570.11467252450430383No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG5490.11302552235702477No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.10232000839971095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG650.045.0000041
AATCCGG351.2099918E-745.0000041
CAACGCA650.045.00000416
ACGCAAG650.045.00000418
ATTCGGC351.2099918E-745.00000416
TAACGGG700.045.0000042
TCTACGG351.2099918E-745.0000041
TGATTGG650.045.0000041
ATACGAA650.045.00000418
GATTCGG351.2099918E-745.00000415
ACATACG700.045.00000416
CTAGCGG750.045.0000041
TCGCCCT207.029261E-445.015
ACAACGG253.8874674E-545.01
CGTGGTT207.029261E-445.027
CGGGTAC207.029261E-445.05
TCCATTA253.8874674E-545.024
ACGCCGG253.8874674E-545.026
GATGTCA207.029261E-445.09
TCCGCAA207.029261E-445.031