##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043287_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	485731
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17640422373701	33.0	31.0	34.0	30.0	34.0
2	32.46196969104299	34.0	31.0	34.0	31.0	34.0
3	32.521473819871495	34.0	31.0	34.0	31.0	34.0
4	36.09036071405778	37.0	35.0	37.0	35.0	37.0
5	36.038945424525096	37.0	35.0	37.0	35.0	37.0
6	36.297712108142164	37.0	37.0	37.0	35.0	37.0
7	36.23367666465595	37.0	37.0	37.0	35.0	37.0
8	36.139737838433206	37.0	37.0	37.0	35.0	37.0
9	37.69200442220077	39.0	38.0	39.0	35.0	39.0
10	37.53929026559968	39.0	37.0	39.0	35.0	39.0
11	37.157523402871135	39.0	35.0	39.0	34.0	39.0
12	37.07757585988953	39.0	37.0	39.0	33.0	39.0
13	37.09696519266837	39.0	37.0	39.0	33.0	39.0
14	38.506541686653726	40.0	37.0	41.0	34.0	41.0
15	38.648727382028326	40.0	37.0	41.0	35.0	41.0
16	38.69735717917942	40.0	38.0	41.0	35.0	41.0
17	38.67827048304514	40.0	37.0	41.0	35.0	41.0
18	38.43400359458219	40.0	37.0	41.0	35.0	41.0
19	38.18061849048136	40.0	37.0	41.0	35.0	41.0
20	37.89358307375893	40.0	35.0	41.0	34.0	41.0
21	37.81099620983631	40.0	35.0	41.0	34.0	41.0
22	37.78854962932158	39.0	35.0	41.0	34.0	41.0
23	37.74689694501689	39.0	35.0	41.0	34.0	41.0
24	37.66308718199991	39.0	35.0	41.0	34.0	41.0
25	37.62998449759229	39.0	35.0	41.0	34.0	41.0
26	37.62378765201315	39.0	35.0	41.0	34.0	41.0
27	37.62908070516397	39.0	35.0	41.0	34.0	41.0
28	37.572808818049495	39.0	35.0	41.0	34.0	41.0
29	37.5805373756256	39.0	35.0	41.0	34.0	41.0
30	37.480043480856686	39.0	35.0	41.0	34.0	41.0
31	37.32133835394488	39.0	35.0	41.0	33.0	41.0
32	37.16951563725601	40.0	35.0	41.0	33.0	41.0
33	36.92275354053993	39.0	35.0	41.0	33.0	41.0
34	36.69781010476992	39.0	35.0	41.0	32.0	41.0
35	36.58446959325223	39.0	35.0	41.0	32.0	41.0
36	36.5076842943934	39.0	35.0	41.0	31.0	41.0
37	36.43961575439904	39.0	35.0	41.0	31.0	41.0
38	36.40528605339169	39.0	35.0	41.0	31.0	41.0
39	36.33651753748474	39.0	35.0	41.0	31.0	41.0
40	36.271514480237	39.0	35.0	41.0	31.0	41.0
41	36.21119714409828	39.0	35.0	41.0	31.0	41.0
42	36.113085226184864	39.0	35.0	41.0	31.0	41.0
43	35.96177719766702	38.0	35.0	41.0	30.0	41.0
44	35.854277367514115	38.0	35.0	40.0	30.0	41.0
45	35.81873711992852	38.0	35.0	40.0	30.0	41.0
46	35.748039552756566	38.0	35.0	40.0	30.0	41.0
47	35.61184894519806	38.0	35.0	40.0	29.0	41.0
48	35.62760869699484	38.0	35.0	40.0	29.0	41.0
49	35.56253358340316	38.0	35.0	40.0	29.0	41.0
50	35.453024410630576	38.0	35.0	40.0	28.0	41.0
51	35.07312071908114	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	6.0
16	5.0
17	22.0
18	74.0
19	152.0
20	320.0
21	475.0
22	778.0
23	1141.0
24	2077.0
25	3892.0
26	5932.0
27	7201.0
28	6846.0
29	6783.0
30	7125.0
31	8518.0
32	10643.0
33	14460.0
34	28671.0
35	56976.0
36	45798.0
37	35428.0
38	62027.0
39	180325.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.795489684619678	20.22086298795012	25.71629152761508	27.267355799815125
2	32.28041858559573	20.339653017822624	27.224121993449046	20.155806403132598
3	21.145654693647305	22.6477206519658	37.10428199970765	19.102342654679237
4	22.601398716573577	24.79767607997019	34.77521508818667	17.825710115269562
5	19.690734171794677	29.735182642244368	30.869143620645996	19.70493956531496
6	73.12421896070047	1.549005519515946	21.2642388482514	4.062536671532185
7	73.91416236558919	1.3476595070110822	17.05120735551159	7.686970771888143
8	60.692646753038204	2.9950734048269516	26.33638783606564	9.975892006069204
9	33.974772044609054	19.00537540325818	28.741422721629874	18.27842983050289
10	28.00665388867501	18.249813168193917	42.111580277972784	11.631952665158288
11	24.6957678221073	16.002272862963245	38.97383531213779	20.328124002791668
12	16.88918351927301	23.38166598384703	42.71932407031876	17.009826426561204
13	11.035326137306452	27.455114044604933	41.65721356059218	19.852346257496436
14	9.45935095762881	22.486932067337683	48.31666086784661	19.737056107186902
15	9.694872264689716	18.40051386466995	51.14600468160361	20.75860918903673
16	10.62151684780259	24.27969390465093	38.75231352332876	26.346475724217726
17	13.3594520423856	21.23994556657903	41.877705973059165	23.522896417976206
18	15.442498008156777	28.780127272090933	39.39732073925691	16.380053980495376
19	16.655926840164618	22.798009597905015	35.703506673446824	24.842556888483543
20	12.015910040742716	31.171780265208522	35.28372700115908	21.528582692889685
21	13.23901501036582	23.932999952648686	35.339725074166566	27.48825996281893
22	10.983445569667163	27.91401001789056	31.549149632203832	29.553394780238445
23	10.691926189598771	23.040530664091854	44.38794312078084	21.879600025528532
24	16.07700558539603	21.527141566010815	40.03326944337503	22.362583405218114
25	10.797128451756219	27.55496354978373	38.29877030702179	23.349137691438266
26	13.970078088489307	25.430330779793753	35.19025962930099	25.409331502415945
27	18.804647016558548	24.365955642114667	37.663439228708896	19.16595811261789
28	12.28766539504376	25.087136707354485	32.918837792934774	29.706360104666985
29	14.60993842270722	30.35569070123175	30.109258004945122	24.925112871115907
30	19.436684090576883	24.804264088559304	26.6375833537493	29.121468467114514
31	17.457399260084287	32.294418103847605	26.562438880779695	23.68574375528842
32	13.655912428895828	28.177736236723618	26.931161486501786	31.235189847878765
33	10.738248124990992	32.48299984971105	28.777245018333193	28.00150700696476
34	10.963475668631403	30.264487957326175	29.69956622080946	29.07247015323296
35	14.733875334289968	28.052358198262002	27.51564137351744	29.69812509393059
36	13.282866442537125	31.79743520590615	31.96069429375519	22.95900405780154
37	12.87379228420669	31.194632420002016	31.915607609973424	24.01596768581787
38	12.187815889864966	27.03101099168058	31.687086062038457	29.094087056416
39	19.08010812569097	23.140380169270646	35.52274818778295	22.256763517255436
40	11.736537301510506	20.63817215701695	40.58707391539762	27.038216626074924
41	16.789128138825813	25.346127795014112	30.37586647753592	27.488877588624156
42	17.46892827511524	18.29572335304932	36.239811747654564	27.995536624180872
43	21.200623390312746	24.316957328233116	32.79984188779386	21.682577393660278
44	13.04734513547622	28.07479860251868	33.096302274304094	25.78155398770101
45	17.229701213222956	25.429919029256936	32.081748951580195	25.258630805939912
46	21.236651562284475	21.76616275263469	32.30862349736788	24.688562187712954
47	15.120920838900545	18.558214320271922	38.042867348388306	28.277997492439233
48	16.91718255577676	16.51037302539883	33.297030661003724	33.27541375782069
49	18.713650147921378	18.09293621366559	38.15506936967169	25.03834426874134
50	16.8055981602986	17.340256232359064	38.14642260839848	27.70772299894386
51	14.816225441653918	16.444287064239262	37.38941924645534	31.350068247651482
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	824.5
2	1496.0
3	16144.5
4	30793.0
5	16922.5
6	3052.0
7	2895.5
8	2739.0
9	2987.5
10	3236.0
11	3389.5
12	3543.0
13	3340.0
14	3137.0
15	2913.0
16	2689.0
17	2384.0
18	2079.0
19	1945.5
20	1812.0
21	1624.0
22	1436.0
23	1437.0
24	1438.0
25	1434.0
26	1606.5
27	1783.0
28	1827.0
29	1871.0
30	2349.5
31	2828.0
32	3199.5
33	3571.0
34	4798.5
35	6026.0
36	7202.0
37	8378.0
38	9718.0
39	11058.0
40	14991.5
41	18925.0
42	27180.0
43	35435.0
44	48559.5
45	61684.0
46	74497.0
47	87310.0
48	82878.0
49	78446.0
50	60625.0
51	42804.0
52	32581.0
53	22358.0
54	17526.5
55	12695.0
56	11224.5
57	9754.0
58	8641.5
59	7529.0
60	6079.5
61	4630.0
62	3660.5
63	2691.0
64	2340.5
65	1990.0
66	1699.5
67	1409.0
68	1175.0
69	941.0
70	829.5
71	718.0
72	735.5
73	753.0
74	669.0
75	439.5
76	294.0
77	199.5
78	105.0
79	101.0
80	97.0
81	56.5
82	16.0
83	10.5
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	485731.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.98637780096884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5076497583437	22.777358066733335
2	11.222255013430745	6.954740672499633
3	4.573487549082684	4.251474391917092
4	2.353289527280864	2.916796734695528
5	1.3024664096968457	2.0179358121968956
6	0.9256342890488436	1.7209232271599193
7	0.6532298792665622	1.4168859480884468
8	0.48075289822226325	1.1917432746580616
9	0.4144162307723397	1.1557132104208683
>10	3.9684608893310545	27.26311284509013
>50	0.41091221204327844	8.483355445868595
>100	0.1761052337328336	9.610693796869214
>500	0.006670652792910364	1.5556194407096888
>1k	0.004002391675746218	2.8069774122824307
>5k	0.0	0.0
>10k+	6.670652792910364E-4	5.876669720810146
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28431	5.853239756161332	No Hit
CCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	3645	0.7504153533540169	No Hit
CTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCT	3321	0.6837117663892154	No Hit
GCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	2933	0.6038321622461815	No Hit
TCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	1412	0.29069587899475224	No Hit
CGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	1201	0.24725619736026733	No Hit
ACCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	1068	0.2198747866617531	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCC	969	0.19949313508917485	No Hit
AGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	948	0.19516975445256737	No Hit
GCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	946	0.1947580039157476	No Hit
ACTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGC	823	0.16943534590133222	No Hit
TCCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	802	0.1651119652647247	No Hit
TGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTGCTT	702	0.14452443842373658	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	641	0.13196604705073384	No Hit
CGGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCT	589	0.12126053309342003	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTC	557	0.11467252450430383	No Hit
GGCTGTCTCTTATACACATCTGACGCACACAACTTCGTATGCCGTCTTCTG	549	0.11302552235702477	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.10232000839971095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7042992932302036	0.0
2	0.0	0.0	0.0	2.571999728244646	0.0
3	0.0	0.0	0.0	4.28014683024143	0.0
4	0.0	0.0	0.0	5.433254208605175	0.0
5	0.0	0.0	0.0	11.250465792794778	0.0
6	0.0	0.0	0.0	12.472335510807422	0.0
7	0.0	0.0	0.0	17.2222897035602	0.0
8	0.0	0.0	0.0	22.978768083568887	0.0
9	0.0	0.0	0.0	27.39643959310812	0.0
10	0.0	0.0	0.0	29.861590057048037	0.0
11	0.0	0.0	0.0	32.11468899452578	0.0
12	0.0	0.0	0.0	33.74542699560045	0.0
13	0.0	0.0	0.0	34.35811179438825	0.0
14	0.0	0.0	0.0	34.81309613757409	0.0
15	0.0	0.0	0.0	36.37321892158417	0.0
16	0.0	0.0	0.0	38.15898099977148	0.0
17	0.0	0.0	0.0	40.50060630266547	0.0
18	0.0	0.0	0.0	41.408104485816224	0.0
19	0.0	0.0	0.0	42.5284776965028	0.0
20	0.0	0.0	0.0	43.653997788899616	0.0
21	6.176258052296436E-4	0.0	0.0	44.622640926768106	0.0
22	0.0014411268788691682	0.0	0.0	45.5997249506414	0.0
23	0.0014411268788691682	0.0	0.0	46.32852340081239	0.0
24	0.004323380636607505	0.0	0.0	46.950472586678636	0.0
25	0.004323380636607505	0.0	0.0	47.49233629313344	0.0
26	0.004323380636607505	0.0	0.0	48.037082253345986	0.0
27	0.004323380636607505	0.0	0.0	48.56844632111189	0.0
28	0.004323380636607505	0.0	0.0	49.13007405333405	0.0
29	0.004323380636607505	0.0	0.0	49.847755239010894	0.0
30	0.004323380636607505	0.0	0.0	50.45817540984619	0.0
31	0.004323380636607505	0.0	0.0	50.9374530347044	0.0
32	0.004323380636607505	0.0	0.0	51.43958281435609	0.0
33	0.004323380636607505	0.0	0.0	51.905066796230834	0.0
34	0.004323380636607505	0.0	0.0	52.516516343408185	0.0
35	0.004323380636607505	0.0	0.0	52.99311758977706	0.0
36	0.004323380636607505	0.0	0.0	53.43863167061604	0.0
37	0.004323380636607505	0.0	0.0	53.89176313638619	0.0
38	0.004323380636607505	0.0	0.0	54.36136462362913	0.0
39	0.004323380636607505	0.0	0.0	54.79782019265808	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	65	0.0	45.000004	1
AATCCGG	35	1.2099918E-7	45.000004	1
CAACGCA	65	0.0	45.000004	16
ACGCAAG	65	0.0	45.000004	18
ATTCGGC	35	1.2099918E-7	45.000004	16
TAACGGG	70	0.0	45.000004	2
TCTACGG	35	1.2099918E-7	45.000004	1
TGATTGG	65	0.0	45.000004	1
ATACGAA	65	0.0	45.000004	18
GATTCGG	35	1.2099918E-7	45.000004	15
ACATACG	70	0.0	45.000004	16
CTAGCGG	75	0.0	45.000004	1
TCGCCCT	20	7.029261E-4	45.0	15
ACAACGG	25	3.8874674E-5	45.0	1
CGTGGTT	20	7.029261E-4	45.0	27
CGGGTAC	20	7.029261E-4	45.0	5
TCCATTA	25	3.8874674E-5	45.0	24
ACGCCGG	25	3.8874674E-5	45.0	26
GATGTCA	20	7.029261E-4	45.0	9
TCCGCAA	20	7.029261E-4	45.0	31
>>END_MODULE