FastQCFastQC Report
Thu 26 May 2016
SRR1043286_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043286_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1379431
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT661114.7926282648425325No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT92710.6720887090401767No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT91710.6648393431784555Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT68130.4938992961590685No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC42800.31027285888166933No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG32000.23197970757507985No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT29330.21262390072428416No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC27050.19609534655955968No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC25070.18174160215335164No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT21490.15578887236838956No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC18850.1366505464934455No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT18420.13353331917290537No Hit
TCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC16980.12309423233202675No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG14990.10866799426720147No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT14740.10685565280177116No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT14670.10634819719145068No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTTG14610.1059132352397474Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC14150.10257852694335563No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC14140.10250603328473841No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC13890.1006936918193081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTA206.311895E-446.0000047
TCGCACT206.311895E-446.0000049
GATCGCG206.311895E-446.0000048
ACCGCAG206.311895E-446.00000431
CCTACGG206.311895E-446.0000041
TAGCCGT206.311895E-446.00000421
CTCGCGG351.01976184E-746.0000041
TCATCGA351.01976184E-746.00000415
ATAACGG700.046.0000041
CGTCAAC206.311895E-446.00000423
CCCATCG206.311895E-446.00000413
CCCGCAC700.046.00000432
CTGTCGG501.6370905E-1146.01
CAACGGC501.6370905E-1146.02
CGCGGAC301.8616756E-646.03
GGTCCGC253.417515E-546.029
CACGAAA253.417515E-546.034
CGGCTGG253.417515E-546.05
CGCACTT650.046.034
TTACCGG650.046.01
Produced by FastQC (version 0.11.5)