##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043286_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1379431
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24761948948516	33.0	31.0	34.0	31.0	34.0
2	32.584145201898465	34.0	31.0	34.0	31.0	34.0
3	32.66734399908368	34.0	31.0	34.0	31.0	34.0
4	36.18007279813198	37.0	35.0	37.0	35.0	37.0
5	36.08322634477549	37.0	35.0	37.0	35.0	37.0
6	36.32430473144362	37.0	37.0	37.0	35.0	37.0
7	36.25936273724456	37.0	37.0	37.0	35.0	37.0
8	36.220842506801716	37.0	37.0	37.0	35.0	37.0
9	37.74547548953155	39.0	38.0	39.0	35.0	39.0
10	37.56052966766732	39.0	37.0	39.0	35.0	39.0
11	37.220140768186305	39.0	37.0	39.0	34.0	39.0
12	37.24887000509631	39.0	37.0	39.0	34.0	39.0
13	37.225786574319415	39.0	37.0	39.0	34.0	39.0
14	38.625304926451555	40.0	38.0	41.0	34.0	41.0
15	38.74316004207532	40.0	38.0	41.0	35.0	41.0
16	38.7829315130659	41.0	38.0	41.0	35.0	41.0
17	38.7427888745432	40.0	38.0	41.0	35.0	41.0
18	38.48273164804909	40.0	38.0	41.0	35.0	41.0
19	38.207338388074504	40.0	37.0	41.0	34.0	41.0
20	37.9163234696045	40.0	35.0	41.0	34.0	41.0
21	37.84980111364759	40.0	35.0	41.0	34.0	41.0
22	37.811741942873546	40.0	35.0	41.0	34.0	41.0
23	37.79703950396939	40.0	35.0	41.0	34.0	41.0
24	37.71721818633915	39.0	35.0	41.0	34.0	41.0
25	37.65080094618723	39.0	35.0	41.0	34.0	41.0
26	37.61592207221673	39.0	35.0	41.0	34.0	41.0
27	37.654602513645116	40.0	35.0	41.0	34.0	41.0
28	37.64902412661453	40.0	35.0	41.0	34.0	41.0
29	37.61229956409563	40.0	35.0	41.0	34.0	41.0
30	37.5370699948022	40.0	35.0	41.0	33.0	41.0
31	37.37976528003213	40.0	35.0	41.0	33.0	41.0
32	37.109456725272956	40.0	35.0	41.0	33.0	41.0
33	36.82628054610923	40.0	35.0	41.0	32.0	41.0
34	36.5723526584512	40.0	35.0	41.0	31.0	41.0
35	36.33371803301506	40.0	35.0	41.0	30.0	41.0
36	36.26253650961882	40.0	35.0	41.0	30.0	41.0
37	36.21132191461552	40.0	35.0	41.0	30.0	41.0
38	36.12615781434519	39.0	35.0	41.0	30.0	41.0
39	36.11623270754391	39.0	35.0	41.0	30.0	41.0
40	36.089599987241115	39.0	35.0	41.0	30.0	41.0
41	36.10369420434947	39.0	35.0	41.0	30.0	41.0
42	36.05578894486205	39.0	35.0	41.0	30.0	41.0
43	35.95822407934866	39.0	35.0	41.0	29.0	41.0
44	35.81544781870206	39.0	35.0	41.0	29.0	41.0
45	35.72586812968535	39.0	35.0	41.0	27.0	41.0
46	35.69973634056361	39.0	35.0	41.0	27.0	41.0
47	35.61885081602487	39.0	35.0	41.0	27.0	41.0
48	35.58619097294464	39.0	35.0	41.0	27.0	41.0
49	35.5336258210813	39.0	35.0	41.0	27.0	41.0
50	35.381948064093095	38.0	35.0	41.0	26.0	41.0
51	35.299986733660475	38.0	35.0	40.0	26.0	41.0
52	34.980550676329585	38.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	2.0
14	3.0
15	20.0
16	49.0
17	106.0
18	252.0
19	453.0
20	867.0
21	1479.0
22	2370.0
23	4372.0
24	8243.0
25	15636.0
26	22140.0
27	23917.0
28	21470.0
29	20249.0
30	21435.0
31	24965.0
32	30867.0
33	40893.0
34	75384.0
35	121416.0
36	120056.0
37	104916.0
38	174081.0
39	542161.0
40	1625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.333059065658233	20.205650010765307	25.44795644001041	26.013334483566048
2	33.880853772316264	19.20248276282032	26.98155978805754	19.93510367680587
3	20.73064908647116	21.461312671674047	38.227501049345705	19.580537192509084
4	22.048728787449317	24.449501279875545	35.90226694919862	17.59950298347652
5	20.076973766719757	29.712685882802404	31.55677957070705	18.65356077977079
6	73.47442532464473	1.8232880078815108	21.08093844490953	3.621348222564231
7	74.57226929074379	1.5318635002403165	17.32214224560707	6.573724963408825
8	61.82868153608263	3.6994238928949685	25.342260685746513	9.129633885275885
9	35.51986289999282	16.573355245749877	29.367181105832767	18.53960074842453
10	27.64168704342588	20.23653230933624	40.59427401588046	11.527506631357422
11	24.185189400557185	16.768000719137095	40.493797805037005	18.553012075268715
12	20.60958467658042	18.88416310783214	42.33709406269687	18.169158152890578
13	15.148129917335481	21.853575858451784	41.98100521156912	21.01728901264362
14	14.149384782566147	22.771273082887074	41.78099520744423	21.29834692710255
15	15.160526332959023	18.82283347264198	45.497672591090094	20.5189676033089
16	16.41807382899181	24.1671384795615	38.02176404619006	21.39302364525663
17	17.16707830982485	21.657915473843925	40.08913820263572	21.085868013695503
18	19.119405030044998	27.513228280356177	38.145945683401344	15.221421006197483
19	18.397730658510646	23.300694271768577	35.97062846927465	22.330946600446126
20	15.93744087235969	29.12229752702382	34.964488981326355	19.975772619290126
21	15.552064583150587	23.854183355310994	37.151550168149036	23.442201893389374
22	14.326776765202464	25.849063853139448	33.02760341039168	26.79655597126641
23	13.701591453287623	23.50679374321731	41.92351774028567	20.868097063209394
24	18.10500126501434	21.862420084803084	38.29354277234599	21.739035877836585
25	14.370127973055558	24.935498767245335	38.66181055812143	22.032562701577678
26	15.52995401727234	21.537865975173823	38.98853947750921	23.943640530044636
27	20.106551179435577	21.442536814092186	40.92846978210581	17.522442224366426
28	15.348357402436221	22.10715867629479	37.61935174720591	24.925132174063076
29	16.74777498838289	21.269204476338434	39.176080572351935	22.80693996292674
30	19.323837147345536	20.848233800748282	36.25299126958869	23.57493778231749
31	18.52763929475269	20.210652073209896	38.90582421302696	22.355884419010447
32	15.381052042472584	21.54837755567332	37.81566457474133	25.254905827112772
33	13.180434541488484	20.932543925720097	41.21097756973709	24.67604396305433
34	17.3997829539861	19.80200531958467	40.13676653634724	22.661445190082
35	15.726629313100837	23.56254136669395	36.27502934180832	24.43579997839689
36	17.110968218055124	24.859815387648965	33.17483803104324	24.854378363252675
37	18.24317417833875	24.457185607688967	35.65549853526563	21.644141678706657
38	16.949524840314595	28.148780185453276	30.24565926095615	24.65603571327598
39	20.57544016337171	26.67875377601344	32.92161768149331	19.824188379121534
40	15.745405170682695	23.401967912856822	38.57982023022536	22.27280668623512
41	17.715347849946824	25.46231018441662	33.96864359290171	22.853698372734847
42	18.090937495242603	20.325989484069883	38.430193318839436	23.152879701848082
43	20.894194780311594	21.737513511005623	35.40720775450168	21.961083954181106
44	15.959623931896557	22.877403799102673	35.94032611997265	25.222646149028115
45	18.158284104097994	21.46479236728767	36.05747587229807	24.319447656316264
46	21.946005273188728	20.685340549835406	34.85270375973862	22.515950417237253
47	15.943602833342155	19.71740521997838	39.073864513701665	25.265127432977803
48	17.002227730129306	18.177277442655704	33.852073789845235	30.968421037369758
49	19.31542788294594	17.68171079234844	38.4077202846681	24.59514104003752
50	17.966538377055468	17.41123695204762	38.985204769212814	25.6370199016841
51	16.025665654896837	17.3024964641218	37.342353477629544	29.32948440335182
52	14.840321842846796	17.478946029196095	44.918738233373034	22.76199389458407
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	444.0
1	1801.5
2	3159.0
3	40888.0
4	78617.0
5	45189.5
6	11762.0
7	11304.0
8	10846.0
9	11147.5
10	11449.0
11	11526.5
12	11604.0
13	11310.0
14	10103.0
15	9190.0
16	8308.5
17	7427.0
18	6764.5
19	6102.0
20	5716.5
21	5331.0
22	5094.5
23	4858.0
24	4729.0
25	4600.0
26	4872.5
27	5145.0
28	5406.0
29	5667.0
30	7027.5
31	8388.0
32	9541.0
33	10694.0
34	13035.0
35	15376.0
36	17756.5
37	20137.0
38	23469.0
39	32736.5
40	38672.0
41	53364.0
42	68056.0
43	97067.5
44	126079.0
45	163811.0
46	201543.0
47	208968.0
48	216393.0
49	189788.5
50	163184.0
51	134259.5
52	105335.0
53	85369.5
54	65404.0
55	53420.0
56	41436.0
57	35605.5
58	29775.0
59	24840.0
60	19905.0
61	16334.5
62	12764.0
63	10379.5
64	6602.5
65	5210.0
66	4111.0
67	3012.0
68	2472.5
69	1933.0
70	1634.5
71	1336.0
72	1321.0
73	1306.0
74	985.5
75	665.0
76	511.0
77	357.0
78	294.0
79	231.0
80	155.0
81	79.0
82	67.0
83	55.0
84	49.0
85	43.0
86	25.0
87	7.0
88	8.5
89	18.0
90	26.0
91	14.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1379431.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.24345234851976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32930635819064	24.044758493199975
2	12.560089871955713	8.350814983029707
3	5.080499876431189	5.0668006664640215
4	2.542138211900495	3.380378020426613
5	1.4490378923194787	2.4085511062261045
6	0.9811696532429607	1.957047996803761
7	0.6772658208654609	1.5760257830255449
8	0.4784810503212723	1.272508959681992
9	0.3843231992100637	1.1498606963433375
>10	2.994534225946979	20.903576283011418
>50	0.3408422994560308	7.642676712853295
>100	0.16613521689595456	10.416131994335812
>500	0.010711349188187934	2.3558109414351884
>1k	0.0045905782235091145	2.835504569185626
>5k	6.557968890727307E-4	1.8352768624204356
>10k+	2.185989630242436E-4	4.804275931557213
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66111	4.7926282648425325	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	9271	0.6720887090401767	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	9171	0.6648393431784555	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	6813	0.4938992961590685	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	4280	0.31027285888166933	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCG	3200	0.23197970757507985	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	2933	0.21262390072428416	No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2705	0.19609534655955968	No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2507	0.18174160215335164	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	2149	0.15578887236838956	No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1885	0.1366505464934455	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	1842	0.13353331917290537	No Hit
TCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1698	0.12309423233202675	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	1499	0.10866799426720147	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	1474	0.10685565280177116	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	1467	0.10634819719145068	No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTTG	1461	0.1059132352397474	Illumina Single End Adapter 1 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1415	0.10257852694335563	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	1414	0.10250603328473841	No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1389	0.1006936918193081	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6792655812432807	0.0
2	0.0	0.0	0.0	2.2228005605209686	0.0
3	0.0	0.0	0.0	3.7379180256207087	0.0
4	0.0	0.0	0.0	4.679683144716916	0.0
5	0.0	0.0	0.0	9.548792219400609	0.0
6	0.0	0.0	0.0	10.712967883134423	0.0
7	0.0	0.0	0.0	14.383394312582507	0.0
8	0.0	0.0	0.0	19.116867751993393	0.0
9	0.0	0.0	0.0	22.847898880045467	0.0
10	0.0	0.0	0.0	25.239537171485924	0.0
11	0.0	0.0	0.0	27.34642037187797	0.0
12	0.0	0.0	0.0	28.770413308095875	0.0
13	0.0	0.0	0.0	29.43264287956411	0.0
14	0.0	0.0	0.0	29.907258862531002	0.0
15	0.0	0.0	0.0	31.3885217890565	0.0
16	0.0	0.0	0.0	33.04043478796692	0.0
17	0.0	0.0	0.0	34.826461055319186	0.0
18	0.0	0.0	0.0	35.90770397359491	0.0
19	0.0	0.0	0.0	37.024323797275834	0.0
20	0.0	0.0	0.0	38.30195203674558	0.0
21	7.249365861721246E-5	0.0	0.0	39.634240494812715	0.0
22	2.1748097585163738E-4	0.0	0.0	40.83495296248961	0.0
23	2.899746344688498E-4	0.0	0.0	41.83674283092086	0.0
24	7.97430244789337E-4	0.0	0.0	42.686441003573215	0.0
25	7.97430244789337E-4	0.0	0.0	43.50366201716505	0.0
26	7.97430244789337E-4	0.0	0.0	44.33153959857361	0.0
27	7.97430244789337E-4	0.0	0.0	45.11650093408079	0.0
28	8.699239034065495E-4	0.0	0.0	45.931184669621025	0.0
29	8.699239034065495E-4	0.0	0.0	46.82626387256775	0.0
30	8.699239034065495E-4	0.0	0.0	47.65979595934846	0.0
31	8.699239034065495E-4	0.0	0.0	48.378208116245034	0.0
32	9.424175620237619E-4	0.0	0.0	49.112278903402924	0.0
33	9.424175620237619E-4	0.0	0.0	49.8323584144477	0.0
34	9.424175620237619E-4	0.0	0.0	50.60042872749706	0.0
35	9.424175620237619E-4	0.0	0.0	51.250769338952075	0.0
36	9.424175620237619E-4	0.0	0.0	51.86152841280209	0.0
37	9.424175620237619E-4	0.0	0.0	52.51128907498816	0.0
38	9.424175620237619E-4	0.0	0.0	53.14365125910611	0.0
39	9.424175620237619E-4	0.0	0.0	53.79580421202655	0.0
40	9.424175620237619E-4	0.0	0.0	54.41692987905883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACTA	20	6.311895E-4	46.000004	7
TCGCACT	20	6.311895E-4	46.000004	9
GATCGCG	20	6.311895E-4	46.000004	8
ACCGCAG	20	6.311895E-4	46.000004	31
CCTACGG	20	6.311895E-4	46.000004	1
TAGCCGT	20	6.311895E-4	46.000004	21
CTCGCGG	35	1.01976184E-7	46.000004	1
TCATCGA	35	1.01976184E-7	46.000004	15
ATAACGG	70	0.0	46.000004	1
CGTCAAC	20	6.311895E-4	46.000004	23
CCCATCG	20	6.311895E-4	46.000004	13
CCCGCAC	70	0.0	46.000004	32
CTGTCGG	50	1.6370905E-11	46.0	1
CAACGGC	50	1.6370905E-11	46.0	2
CGCGGAC	30	1.8616756E-6	46.0	3
GGTCCGC	25	3.417515E-5	46.0	29
CACGAAA	25	3.417515E-5	46.0	34
CGGCTGG	25	3.417515E-5	46.0	5
CGCACTT	65	0.0	46.0	34
TTACCGG	65	0.0	46.0	1
>>END_MODULE