##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043285_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1363233
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19108618996166	33.0	31.0	34.0	31.0	34.0
2	32.495171405034945	34.0	31.0	34.0	31.0	34.0
3	32.56899957674147	34.0	31.0	34.0	31.0	34.0
4	36.13889261776967	37.0	35.0	37.0	35.0	37.0
5	36.0982700682862	37.0	35.0	37.0	35.0	37.0
6	36.329066271136334	37.0	37.0	37.0	35.0	37.0
7	36.273301775998675	37.0	37.0	37.0	35.0	37.0
8	36.160922600905344	37.0	37.0	37.0	35.0	37.0
9	37.69478071613583	39.0	38.0	39.0	35.0	39.0
10	37.50676370070267	39.0	37.0	39.0	35.0	39.0
11	37.21800968726549	39.0	37.0	39.0	34.0	39.0
12	37.255401681150616	39.0	37.0	39.0	34.0	39.0
13	37.248088918035286	39.0	37.0	39.0	34.0	39.0
14	38.638840168921966	40.0	38.0	41.0	34.0	41.0
15	38.765947567290404	40.0	38.0	41.0	35.0	41.0
16	38.82489567080609	40.0	38.0	41.0	35.0	41.0
17	38.76784746261277	40.0	38.0	41.0	35.0	41.0
18	38.541876553751266	40.0	38.0	41.0	35.0	41.0
19	38.29266970503208	40.0	37.0	41.0	35.0	41.0
20	38.02406851946806	40.0	35.0	41.0	34.0	41.0
21	37.95804092183801	40.0	35.0	41.0	34.0	41.0
22	37.929251272526415	40.0	35.0	41.0	34.0	41.0
23	37.88593806047829	40.0	35.0	41.0	34.0	41.0
24	37.81011169770685	40.0	35.0	41.0	34.0	41.0
25	37.80932826596774	40.0	35.0	41.0	34.0	41.0
26	37.80270210594961	40.0	35.0	41.0	34.0	41.0
27	37.81656914115195	40.0	35.0	41.0	34.0	41.0
28	37.7463074910892	40.0	35.0	41.0	34.0	41.0
29	37.75675544826159	40.0	35.0	41.0	34.0	41.0
30	37.636550024830676	40.0	35.0	41.0	34.0	41.0
31	37.47273796922463	40.0	35.0	41.0	33.0	41.0
32	37.211287432155764	40.0	35.0	41.0	33.0	41.0
33	36.89757656981602	40.0	35.0	41.0	32.0	41.0
34	36.60411756464229	40.0	35.0	41.0	31.0	41.0
35	36.36714780231993	40.0	35.0	41.0	30.0	41.0
36	36.27836107253859	40.0	35.0	41.0	30.0	41.0
37	36.174004003717634	39.0	35.0	41.0	30.0	41.0
38	36.078722419425	39.0	35.0	41.0	30.0	41.0
39	36.017698368510736	39.0	35.0	41.0	29.0	41.0
40	35.9743763538588	39.0	35.0	41.0	29.0	41.0
41	35.960761659965684	39.0	35.0	41.0	29.0	41.0
42	35.90144384708997	39.0	35.0	41.0	29.0	41.0
43	35.787761153082414	39.0	35.0	41.0	28.0	41.0
44	35.7064206925742	39.0	35.0	41.0	28.0	41.0
45	35.64493303785927	39.0	35.0	41.0	27.0	41.0
46	35.55816650565237	39.0	35.0	41.0	27.0	41.0
47	35.41496427976729	38.0	35.0	40.0	26.0	41.0
48	35.40649544135155	38.0	35.0	40.0	26.0	41.0
49	35.343115226817424	38.0	35.0	40.0	26.0	41.0
50	35.22269120539189	38.0	35.0	40.0	26.0	41.0
51	34.81633367150003	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	9.0
15	14.0
16	41.0
17	73.0
18	198.0
19	376.0
20	713.0
21	1234.0
22	2035.0
23	3595.0
24	6431.0
25	13174.0
26	20588.0
27	23885.0
28	22769.0
29	20859.0
30	21947.0
31	25145.0
32	30370.0
33	41243.0
34	75229.0
35	119850.0
36	115796.0
37	106262.0
38	188310.0
39	522922.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.76705596182017	20.303939238560098	25.54955755912599	25.379447240493736
2	33.678101982566446	19.295380907005626	26.90141743927854	20.125099671149393
3	20.845005952760825	21.59667496312076	37.8338112413652	19.72450784275322
4	22.222760159121734	24.570341240272207	35.48153543818262	17.725363162423445
5	20.139183837245724	30.019446418917383	31.10429398349365	18.737075760343245
6	73.92932829530974	1.7859749580592605	20.620979685791056	3.66371706083993
7	74.9853473324076	1.4990100738465104	16.922272274805554	6.593370318940342
8	62.08087685670755	3.643544427108205	24.967485382176047	9.3080933340082
9	35.69206437931007	16.60273775649504	29.058128727811024	18.647069136383877
10	27.93366944608882	20.194566886218276	40.23809576205975	11.633667905633153
11	24.30105491871162	16.74651361872842	40.310863953557465	18.641567509002495
12	20.900682421860388	18.680078900672154	42.11026288242729	18.308975795040173
13	15.281320214519456	21.785564169881454	41.767328108987975	21.16578750661112
14	14.255009965281062	22.67440708961711	41.493640485522285	21.57694245957954
15	15.175248838606459	18.821213981762472	45.329448450851764	20.674088728779306
16	16.47260593016748	24.070646763979454	37.92748561691215	21.529261688940924
17	17.300710883612705	21.65242478725207	39.87960972188907	21.167254607246157
18	19.190849986759417	27.550756180344816	38.05006187496928	15.208331957926488
19	18.476592042592866	23.300859060776844	35.708055776231944	22.514493120398345
20	15.976652560494061	29.133611055483545	34.71820297777416	20.171533406248233
21	15.653743710722964	23.84603365675567	36.86347088135337	23.636751751167996
22	14.46238464004319	25.86036282865805	32.620982619992326	27.05626991130643
23	13.77182037113245	23.412285354007714	41.69257933163296	21.123314943226873
24	18.23562076328845	21.76766554213403	38.03861849001601	21.95809520456151
25	14.295355232744514	24.824589780323684	38.62993340096667	22.25012158596513
26	15.610024111798937	21.306335747447427	38.84420344871346	24.239436692040172
27	20.204323105441258	21.319759718257995	40.75216782457584	17.72374935172491
28	15.444975290357554	22.094462208587967	37.35252887804212	25.108033623012354
29	16.898505244517995	21.194836099184805	38.91616473486191	22.99049392143529
30	19.467471811495173	20.797178472058704	35.97074014493487	23.76460957151125
31	18.55332140580517	20.160823571612482	38.58907464828096	22.696780374301383
32	15.448863107040395	21.361938861515238	37.61220569044323	25.576992341001137
33	13.262589740711968	20.75580623415073	41.09833021941224	24.883273805725068
34	17.519602298359853	19.60075790418806	40.06930583399903	22.810333963453054
35	15.678244291328042	23.443974727724463	36.28939440286437	24.588386578083128
36	17.188771105159574	24.61692168543455	33.00616989172064	25.18813731768524
37	18.475931847307102	24.198798004449717	35.45798847299031	21.867281675252872
38	17.16273006888771	27.981570281822698	30.002281341487482	24.853418307802112
39	20.789549548756522	26.590905589873486	32.625530631960935	19.99401422940906
40	15.762749287906031	23.312742575920627	38.52158801906938	22.40292011710397
41	17.912785268549104	25.271762053882206	33.85518102921511	22.960271648353583
42	18.244716787225663	20.095464238321696	38.42387911677608	23.23593985767657
43	20.994650217534346	21.524640321940563	35.293819911929944	22.18688954859514
44	16.121308683108463	22.499455338889245	36.02766364957421	25.351572328428084
45	18.243763171812887	21.198577205804142	36.0273702294472	24.530289392935764
46	22.175739583768877	20.535887848959057	34.6731629882786	22.61520957899347
47	16.168035838334312	19.537599221849824	38.85821425977804	25.436150680037823
48	17.194639507699712	17.911831653136332	33.67582797658214	31.21770086258182
49	19.520727564546927	17.412430596970584	38.25450234846134	24.81233949002115
50	18.080254806038294	17.080865853452785	38.99678191475705	25.84209742575187
51	16.21769719483023	17.172046157920178	37.04869233652648	29.56156431072311
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	423.0
1	1756.5
2	3090.0
3	38777.0
4	74464.0
5	43228.5
6	11993.0
7	11375.0
8	10757.0
9	10925.5
10	11094.0
11	11039.0
12	10984.0
13	10559.0
14	10134.0
15	9232.0
16	8330.0
17	7597.0
18	6864.0
19	6178.5
20	5493.0
21	5088.0
22	4683.0
23	4588.5
24	4494.0
25	4338.0
26	4524.5
27	4867.0
28	5607.5
29	6348.0
30	7585.0
31	8822.0
32	9869.0
33	10916.0
34	13800.5
35	16685.0
36	19619.5
37	22554.0
38	27256.0
39	31958.0
40	40220.5
41	48483.0
42	63870.0
43	79257.0
44	113654.0
45	148051.0
46	185760.0
47	223469.0
48	219829.5
49	216190.0
50	176704.0
51	137218.0
52	110887.5
53	84557.0
54	68843.5
55	53130.0
56	44337.5
57	35545.0
58	30445.0
59	25345.0
60	20705.0
61	16065.0
62	12882.0
63	9699.0
64	8044.5
65	6390.0
66	5091.5
67	3793.0
68	3046.0
69	2299.0
70	1920.0
71	1541.0
72	1405.0
73	1269.0
74	1008.5
75	621.5
76	495.0
77	361.0
78	227.0
79	166.0
80	105.0
81	95.5
82	86.0
83	60.5
84	35.0
85	38.5
86	42.0
87	31.5
88	21.0
89	26.0
90	31.0
91	17.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1363233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.41496891070268
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46104847147782	23.16407664529171
2	12.711257387938483	8.240700260919315
3	5.2834284879711975	5.137865105385217
4	2.6596896516997828	3.448550294878644
5	1.539603031544056	2.4953092201162086
6	1.020487943529774	1.9847450977958723
7	0.693153436714543	1.572798297101404
8	0.5241973804332698	1.359347343185298
9	0.3861274154853544	1.1264677351674968
>10	3.1824611551868083	21.484384280489444
>50	0.3467911994460753	7.569892776875616
>100	0.17417694864039737	10.563754154750292
>500	0.012098791771650462	2.6145957252981433
>1k	0.004565581800622816	2.7880437464703527
>5k	6.848372700934224E-4	1.83681750407754
>10k+	2.282790900311408E-4	4.61265181219746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	62336	4.572659259275561	No Hit
CTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCT	8902	0.6530064926538603	No Hit
CCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	8887	0.6519061671775844	No Hit
GCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	7034	0.5159792933416371	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	4066	0.29826155910251584	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCC	3051	0.22380620187451447	No Hit
TCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2948	0.21625063360408678	No Hit
ACCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	2611	0.19152998790375528	No Hit
GCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	2587	0.18976946714171386	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	2144	0.15727318807569945	No Hit
AGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	2013	0.14766367891622342	No Hit
ACTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	1800	0.13203905715310588	No Hit
TGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGCTT	1779	0.13049860148631964	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	1720	0.12617065461296784	No Hit
GGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	1481	0.10863880202430547	No Hit
TGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTG	1459	0.10702499132576748	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	1435	0.10526447056372608	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTC	1398	0.10255033438891223	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCT	1370	0.10049639349986393	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	1366	0.10020297337285702	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6671640137819433	0.0
2	0.0	0.0	0.0	2.219283130616703	0.0
3	0.0	0.0	0.0	3.7447743709255863	0.0
4	0.0	0.0	0.0	4.708806198206763	0.0
5	0.0	0.0	0.0	9.605181212602687	0.0
6	0.0	0.0	0.0	10.793386016917138	0.0
7	0.0	0.0	0.0	14.525763387476681	0.0
8	0.0	0.0	0.0	19.386267791346015	0.0
9	0.0	0.0	0.0	23.21730767961163	0.0
10	0.0	0.0	0.0	25.709104753185994	0.0
11	0.0	0.0	0.0	27.854519366828708	0.0
12	0.0	0.0	0.0	29.30306117883003	0.0
13	0.0	0.0	0.0	29.981228447374733	0.0
14	0.0	0.0	0.0	30.45979667452299	0.0
15	0.0	0.0	0.0	31.982280358530055	0.0
16	0.0	0.0	0.0	33.685070710582856	0.0
17	0.0	0.0	0.0	35.5277491081862	0.0
18	0.0	0.0	0.0	36.64509295182848	0.0
19	0.0	0.0	0.0	37.81151131171267	0.0
20	0.0	0.0	0.0	39.15104754653093	0.0
21	0.0	0.0	0.0	40.47063121271272	0.0
22	2.93420127006902E-4	0.0	0.0	41.72118779401614	0.0
23	4.4013019051035294E-4	0.0	0.0	42.745590812428986	0.0
24	0.001173680508027608	0.0	0.0	43.63392024694238	0.0
25	0.001173680508027608	0.0	0.0	44.46561959694344	0.0
26	0.001173680508027608	0.0	0.0	45.30370083470691	0.0
27	0.001173680508027608	0.0	0.0	46.097769053419334	0.0
28	0.001173680508027608	0.0	0.0	46.917364823181366	0.0
29	0.001173680508027608	0.0	0.0	47.80642780801227	0.0
30	0.001173680508027608	0.0	0.0	48.64854357252209	0.0
31	0.001173680508027608	0.0	0.0	49.36786301387951	0.0
32	0.001173680508027608	0.0	0.0	50.111389615715	0.0
33	0.001173680508027608	0.0	0.0	50.83239622280271	0.0
34	0.001173680508027608	0.0	0.0	51.6014503756878	0.0
35	0.001173680508027608	0.0	0.0	52.26105882119931	0.0
36	0.001173680508027608	0.0	0.0	52.88164238981891	0.0
37	0.001173680508027608	0.0	0.0	53.537069598520574	0.0
38	0.001173680508027608	0.0	0.0	54.17540508482409	0.0
39	0.001173680508027608	0.0	0.0	54.82980532308123	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	7.0337486E-4	45.000004	12
CTTCGAC	25	3.891188E-5	45.000004	21
GTATTCG	20	7.0337486E-4	45.000004	8
CTATCGG	50	2.1827873E-11	45.000004	1
CGCATGA	20	7.0337486E-4	45.000004	25
CGACATC	20	7.0337486E-4	45.000004	10
CCGTACT	20	7.0337486E-4	45.000004	24
TCGTGGA	20	7.0337486E-4	45.000004	20
TGATTCG	20	7.0337486E-4	45.000004	45
GATATCG	25	3.891188E-5	45.000004	8
TACTCCG	20	7.0337486E-4	45.000004	21
TTACGAG	20	7.0337486E-4	45.000004	20
CGACTCT	20	7.0337486E-4	45.000004	21
CGATCCG	25	3.891188E-5	45.000004	9
TCGACAT	20	7.0337486E-4	45.000004	18
TACCGGC	20	7.0337486E-4	45.000004	2
GCGATCC	45	3.8562575E-10	45.0	8
ACGTAGG	140	0.0	45.0	1
TTACCGG	70	0.0	45.0	1
CGACGGT	110	0.0	44.999996	27
>>END_MODULE